Selection of landcover types by translocated female eastern wild turkeys in east Texas

Restoration of eastern wild turkeys (Meleagris gallopavo silvestris) is among the greatest conservation achievements in North America. However, restoration efforts in east Texas have had limited success, resulting in a fragmented distribution of turkeys across the landscape. Restoration success is largely dependent on the ability of translocated individuals to quickly select habitat patches on the landscape. Information on habitat selection of translocated wild turkeys is important to identify high quality release locations that should reduce the probability of translocation failure. Our objective was to describe selection of landcover types by translocated female wild turkeys in east Texas. During 2016–2017, we translocated 78 GPS‐tagged wild turkeys to Angelina National Forest in east Texas. We quantified third order selection for females during 3 temporal periods—the exploratory phase (days 1–20), the exploitation phase (days 21–80), and within the annual range. During the exploratory phase, females selected shrub‐scrub and open landcover types, as well as linear paths (roads, rights-of‐way). During the exploitation phase, females selected shrub-scrub, mixed, and open landcover types, but not linear paths. Overall, wild turkeys translocated into forested landscapes of east Texas selected for early successional and other open landcover types. Prior to translocation attempts in forest-dominated landscapes, managers should consider ways to increase availability of open landcover types with herbaceous cover for nesting and brood rearing

Catastrophic thinking about pain: A critical appraisal highlighting the importance of the social context and balance

Numerous research studies have shown that endorsing a catastrophic interpretation about pain is associated with deleterious outcomes, such as higher levels of distress, pain intensity and disability for the person in pain. The fear-avoidance model has been found to be useful in explaining these associations by stressing that heightened feelings of distress and behaviour aimed at reducing or avoiding pain might be adaptive in an acute pain context but can become maladaptive when the pain becomes chronic. Pain is rarely a private event and the communal coping model underscores that the heightened pain expression in people endorsing catastrophic thoughts about pain could have a social, communicative function of eliciting empathic responses in others. However, these models are not all-encompassing. In particular, neither of the models takes into account the growing evidence indicating that catastrophic thinking in observers can also impact their emotional experience and behaviour in response to the other’s pain. Moreover, the context of multiple goals in which pain and pain behaviour occurs is largely ignored in both models. In this article we present an integrative perspective on catastrophic thinking that takes into account the social system and interplay between different goals people in pain and observers might pursue (e.g., school/work performance, leisure, social engagement). Specifically, this integrative perspective stresses the importance of considering the bidirectional influence between catastrophic thoughts in the person experiencing pain and observers. Furthermore, the importance of balance between pain-relief and other important goals as well as in the level of catastrophic thoughts in understanding the maladaptive influence of catastrophic thinking will be underlined. Clinical implications and future research directions of this integrated perspective are discussed

Microscale Quantification of the Absorption by Dissolved and Particulate Material in Coastal Waters with an ac-9

Measuring coastal and oceanic absorption coefficients of dissolved and particulate matter in the visible domain usually requires a methodology for amplifying the natural signal because conventional spectrophotometers lack the necessary sensitivity. The WET Labs ac-9 is a recently developed in situ absorption and attenuation meter with a precision better than ±0.001 m−1 in the raw signal, which is sufficient to make these measurements in pristine samples. Whereas the superior sensitivity of the ac-9 has been well documented, the accuracy of in situ measurements for bio-optical applications has not been rigorously evaluated. Obtaining accurate results with an ac-9 requires careful attention to calibration procedures because baselines drift as a result of the changing optical properties of several ac-9 components. To correct in situ measurements for instrument drift, a pressurized flow procedure was developed for calibrating an ac-9 with optically clean water. In situ, micro- (cm) to fine- (m) scale vertical profiles of spectral total absorption, at(λ), and spectral absorption of dissolved materials, ag(λ), were then measured concurrently using multiple meters, corrected for drift, temperature, salinity, and scattering errors and subsequently compared. Particulate absorption, ap(λ), was obtained from at(λ) − ag(λ). CTD microstructure was simultaneously recorded. Vertical profiles of ag(λ), at(λ), and ap(λ) were replicated with different meters within ±0.005 m−1, and spectral relationships compared well with laboratory measurements and hydrographic structure

Elp3 and RlmN: A tale of two mitochondrial tail-anchored radical SAM enzymes in Toxoplasma gondii

Radical S-adenosylmethionine (rSAM) enzymes use a 5'-deoxyadensyl 5'-radical to methylate a wide array of diverse substrates including proteins, lipids and nucleic acids. One such enzyme, Elongator protein-3 (TgElp3), is an essential protein in Toxoplasma gondii, a protozoan parasite that can cause life-threatening opportunistic disease. Unlike Elp3 homologues which are present in all domains of life, TgElp3 localizes to the outer mitochondrial membrane (OMM) via a tail-anchored trafficking mechanism in Toxoplasma. Intriguingly, we identified a second tail-anchored rSAM domain containing protein (TgRlmN) that also localizes to the OMM. The transmembrane domain (TMD) on Toxoplasma Elp3 and RlmN homologues is required for OMM localization and has not been seen beyond the chromalveolates. Both TgElp3 and TgRlmN contain the canonical rSAM amino acid sequence motif (CxxxCxxC) necessary to form the 4Fe-4S cluster required for tRNA modifications. In E. coli, RlmN is responsible for the 2-methlyadenosine (m2A) synthesis at purine 37 in tRNA while in S. cerevisiae, Elp3 is necessary for the formation of 5-methoxycarbonylmethyl-2-thiouridine (mcm5s2U) at the wobble tRNA position. To investigate why these two rSAM enzymes localize to the mitochondrion in Toxoplasma, and whether or not TgRlmN and TgElp3 possess tRNA methyltransferase activity, a series of mutational and biochemical studies were performed. Overexpression of either TgElp3 or TgRlmN resulted in a significant parasite replication defect, but overexpression was tolerated if either the TMD or rSAM domain was mutated. Furthermore, we show the first evidence that Toxoplasma tRNAGlu contains the mcm5s2U modification, which is the putative downstream product generated by TgElp3 activity