A [4Fe-4S]-Fe(CO)(CN)-L-cysteine intermediate is the first organometallic precursor in [FeFe] hydrogenase H-cluster bioassembly.

Biosynthesis of the [FeFe] hydrogenase active site (the 'H-cluster') requires the interplay of multiple proteins and small molecules. Among them, the radical S-adenosylmethionine enzyme HydG, a tyrosine lyase, has been proposed to generate a complex that contains an Fe(CO)2(CN) moiety that is eventually incorporated into the H-cluster. Here we describe the characterization of an intermediate in the HydG reaction: a [4Fe-4S][(Cys)Fe(CO)(CN)] species, 'Complex A', in which a CO, a CN- and a cysteine (Cys) molecule bind to the unique 'dangler' Fe site of the auxiliary [5Fe-4S] cluster of HydG. The identification of this intermediate-the first organometallic precursor to the H-cluster-validates the previously hypothesized HydG reaction cycle and provides a basis for elucidating the biosynthetic origin of other moieties of the H-cluster

Lack of trust in maternal support is associated with negative interpretations of ambiguous maternal behavior

Attachment theory assumes that children who lack trust in maternal availability for support are more inclined to interpret maternal behavior in congruence with their expectation that mother will remain unavailable for support. To provide the first test of this assumption, early adolescents (9-13 years old) were asked to assess whether ambiguous interactions with mother should be interpreted in a positive or a negative way. In our sample (n = 322), results showed that early adolescents' lack of trust in their mother's availability for support was related to more negative interpretations of maternal behavior. The associations remained significant after controlling for depressive mood. The importance of these findings for our understanding of attachment theory, attachment stability, and clinical practice are discussed

A clinical diagnostic model for predicting influenza among young adult military personnel with febrile respiratory illness in Singapore

10.1371/journal.pone.0017468PLoS ONE63

Attachment as an organizer of behavior: implications for substance abuse problems and willingness to seek treatment

BACKGROUND: Attachment theory allows specific predictions about the role of attachment representations in organizing behavior. Insecure attachment is hypothesized to predict maladaptive emotional regulation whereas secure attachment is hypothesized to predict adaptive emotional regulation. In this paper, we test specific hypotheses about the role of attachment representations in substance abuse/dependence and treatment participation. Based on theory, we expect divergence between levels of maladaptive functioning and adaptive methods of regulating negative emotions. METHODS: Participants for this study consist of a sample of adoptees participating in an ongoing longitudinal adoption study (n = 208). The Semi-Structured Assessment of the Genetics of Alcohol-II [41] was used to determine lifetime substance abuse/dependence and treatment participation. Attachment representations were derived by the Adult Attachment Interview [AAI; [16]]. We constructed a prior contrasts reflecting theoretical predictions for the association between attachment representations, substance abuse/dependence and treatment participation. RESULTS: Logistic regression was used to test our hypotheses. As predicted, individuals classified as dismissing, preoccupied or earned-secure reported the highest rates of substance abuse/dependence. Individuals classified as dismissing reported significantly lower rates of treatment participation despite their high rates of substance abuse/dependence. As expected, the continuous-secure group reported lowest rates of both substance abuse/dependence and treatment participation. CONCLUSION: The findings from this study identify attachment representations as an influential factor in understanding the divergence between problematic substance use and treatment utilization. The findings further imply that treatment may need to take attachment representations into account to promote successful recovery

JWST UNCOVER: Extremely Red and Compact Object at z phot ≃ 7.6 Triply Imaged by A2744

Recent JWST/NIRCam imaging taken for the ultra-deep UNCOVER program reveals a very red dropout object at z phot ≃ 7.6, triply imaged by the galaxy cluster A2744 (z d = 0.308). All three images are very compact, i.e., unresolved, with a delensed size upper limit of r e ≲ 35 pc. The images have apparent magnitudes of m F444W ∼ 25−26 AB, and the magnification-corrected absolute UV magnitude of the source is M UV,1450 = −16.81 ± 0.09. From the sum of observed fluxes and from a spectral energy distribution (SED) analysis, we obtain estimates of the bolometric luminosities of the source of L bol ≳ 1043 erg s−1 and L bol ∼ 1044–1046 erg s−1, respectively. Based on its compact, point-like appearance, its position in color–color space, and the SED analysis, we tentatively conclude that this object is a UV-faint dust-obscured quasar-like object, i.e., an active galactic nucleus at high redshift. We also discuss other alternative origins for the object’s emission features, including a massive star cluster, Population III, supermassive, or dark stars, or a direct-collapse black hole. Although populations of red galaxies at similar photometric redshifts have been detected with JWST, this object is unique in that its high-redshift nature is corroborated geometrically by lensing, that it is unresolved despite being magnified—and thus intrinsically even more compact—and that it occupies notably distinct regions in both size–luminosity and color–color space. Planned UNCOVER JWST/NIRSpec observations, scheduled in Cycle 1, will enable a more detailed analysis of this object

JEMS: A Deep Medium-band Imaging Survey in the Hubble Ultra Deep Field with JWST NIRCam and NIRISS

We present JWST Extragalactic Medium-band Survey, the first public medium-band imaging survey carried out using JWST/NIRCam and NIRISS. These observations use ∼2 and ∼4 μm medium-band filters (NIRCam F182M, F210M, F430M, F460M, F480M; and NIRISS F430M and F480M in parallel) over 15.6 arcmin2 in the Hubble Ultra Deep Field (UDF), thereby building on the deepest multiwavelength public data sets available anywhere on the sky. We describe our science goals, survey design, NIRCam and NIRISS image reduction methods, and describe our first data release of the science-ready mosaics, which reach 5σ point-source limits (AB mag) of ∼29.3-29.4 in 2 μm filters and ∼28.2-28.7 at 4 μm. Our chosen filters create a JWST imaging survey in the UDF that enables novel analysis of a range of spectral features potentially across the redshift range of 0.3 1 mag) across redshifts 1.5 < z < 9.3, most prominently Hα+[N ii] and [O iii]+Hβ. We present our first data release including science-ready mosaics of each medium-band image available to the community, adding to the legacy value of past and future surveys in the UDF. This survey demonstrates the power of medium-band imaging with JWST, informing future extragalactic survey strategies using JWST observations

A recently quenched galaxy 700 million years after the Big Bang

Local and low-redshift (z  1010 M⊙) and relatively old. Here we report a (mini-)quenched galaxy at z = 7.3, when the Universe was only 700 Myr old. The JWST/NIRSpec spectrum is very blue (U–V = 0.16 ± 0.03 mag) but exhibits a Balmer break and no nebular emission lines. The galaxy experienced a short starburst followed by rapid quenching; its stellar mass (4–6 × 108 M⊙) falls in a range that is sensitive to various feedback mechanisms, which can result in perhaps only temporary quenching

JADES Imaging of GN-z11: Revealing the Morphology and Environment of a Luminous Galaxy 430 Myr after the Big Bang

We present JWST NIRCam nine-band near-infrared imaging of the luminous z = 10.6 galaxy GN-z11 from the JWST Advanced Deep Extragalactic Survey of the GOODS-N field. We find a spectral energy distribution (SED) entirely consistent with the expected form of a high-redshift galaxy: a clear blue continuum from 1.5 to 4 μm with a complete dropout in F115W. The core of GN-z11 is extremely compact in JWST imaging. We analyze the image with a two-component model, using a point source and a Sérsic profile that fits to a half-light radius of 200 pc and an index n = 0.9. We find a low-surface-brightness haze about 0.″4 to the northeast of the galaxy, which is most likely a foreground object but might be a more extended component of GN-z11. At a spectroscopic redshift of 10.60 (Bunker et al. 2023), the comparison of the NIRCam F410M and F444W images spans the Balmer jump. From population-synthesis modeling, here assuming no light from an active galactic nucleus, we reproduce the SED of GN-z11, finding a stellar mass of ∼109 M ⊙, a star formation rate of ∼20 M ⊙ yr−1, and a young stellar age of ∼20 Myr. Since massive galaxies at high redshift are likely to be highly clustered, we search for faint neighbors of GN-z11, finding nine galaxies out to ∼5 comoving Mpc transverse with photometric redshifts consistent with z = 10.6, and a tenth more tentative dropout only 3″ away. This is consistent with GN-z11 being hosted by a massive dark-matter halo (≈8 × 1010 M ⊙), though lower halo masses cannot be ruled out