Serum vitamin D in patients with mild cognitive impairment and Alzheimer's disease

Objectives: To determine the relevance of Mini-Mental State Examination (MMSE), serum 25-hydroxyvitamin D (25(OH)D3), and 1,25(OH)2D3 concentrations to mild cognitive impairment (MCI) and various stages of Alzheimer's disease (AD). Materials and Methods: The study included 230 participants (>74 years) allocated to three main groups: 1-healthy subjects (HS, n = 61), 2-patients with MCI (n = 61), and 3- patients with Alzheimer's disease (AD) subdivided into three stages: mild (n = 41), moderate (n = 35), and severe AD (n = 32). The cognitive status was evaluated using MMSE. Serum 25 (OH)D3 (ng/ml) and 1,25(OH)2D3 concentrations (pg/ml) were determined by competitive radioimmunoassay. Results: MMSE scores and 25(OH)D3 were decreased in MCI and all stages of the AD in both genders. MMSE variability was due to gender in HS (11%) and to 25(OH)D3 in MCI (15%) and AD (26%). ROC analysis revealed an outstanding property of MMSE in diagnosis of MCI (AUC, 0.906; CI 95%, 0.847–0.965; sensitivity 82%; specificity, 98%) and AD (AUC, 0.997; CI 95%, 0.992–1; sensitivity, 100%; specificity, 98%). 25(OH)D3 exhibited good property in MCI (AUC, 0.765; CI 95%, 0.681–0.849; sensitivity, 90%; specificity, 54%) and an excellent property in diagnosis of AD (AUC, 0.843; CI 95%, 0.782–0.904; sensitivity, 97%; specificity, 79%). Logistic analyses revealed that, in MCI, MMSE could predict (or classify correctly) with 97.6% accuracy (Wald, 15.22, β, −0.162; SE, 0.554; OR = 0.115:0.039–0.341; p =.0001), whereas 25(OH)D3 with 80% accuracy (Wald, 41,013; β, −0.213; SE, 0.033; OR = 0.808: 0.757–863; p =.0001). 25(OH)D3 was the only significant predictor for the severe AD and contributed to MMSE variability. Age and gender were significant predictors only in the moderate AD. In patients with MCI, 25(OH)D3 and 1,25(OH)2D3 were correlated men, but in case of the AD, they were correlated in women. Conclusions: MMSE and serum 25(OH)D3 concentrations could be useful biomarkers for prediction and diagnosis of MCI and various stages of the AD. The results support the utility of vitamin D supplementation in AD therapy regimen. © 2018 The Authors. Brain and Behavior published by Wiley Periodicals, Inc

Exchange biasing of single-domain Ni nanoparticles spontaneously grown in an antiferromagnetic MnO matrix

Exchange biased composites of ferromagnetic single-domain Ni nanoparticles embedded within large grains of MnO have been prepared by reduction of Ni$_x$Mn$_{1-x}$O$_4$ phases in flowing hydrogen. The Ni precipitates are 15-30 nm in extent, and the majority are completely encased within the MnO matrix. The manner in which the Ni nanoparticles are spontaneously formed imparts a high ferromagnetic- antiferromagnetic interface/volume ratio, which results in substantial exchange bias effects. Exchange bias fields of up to 100 Oe are observed, in cases where the starting Ni content $x$ in the precursor Ni$_x$Mn$_{1-x}$O$_4$ phase is small. For particles of approximately the same size, the exchange bias leads to significant hardening of the magnetization, with the coercive field scaling nearly linearly with the exchange bias field.Comment: 6 pages PDFLaTeX with 9 figure

Structural Insights into Differences in Drug-binding Selectivity between Two Forms of Human α1-Acid Glycoprotein Genetic Variants, the A and F1*S Forms

Human α1-acid glycoprotein (hAGP) in serum functions as a carrier of basic drugs. In most individuals, hAGP exists as a mixture of two genetic variants, the F1*S and A variants, which bind drugs with different selectivities. We prepared a mutant of the A variant, C149R, and showed that its drug-binding properties were indistinguishable from those of the wild type. In this study, we determined the crystal structures of this mutant hAGP alone and complexed with disopyramide (DSP), amitriptyline (AMT), and the nonspecific drug chlorpromazine (CPZ). The crystal structures revealed that the drug-binding pocket on the A variant is located within an eight-stranded β-barrel, similar to that found in the F1*S variant and other lipocalin family proteins. However, the binding region of the A variant is narrower than that of the F1*S variant. In the crystal structures of complexes with DSP and AMT, the two aromatic rings of each drug interact with Phe-49 and Phe-112 at the bottom of the binding pocket. Although the structure of CPZ is similar to those of DSP and AMT, its fused aromatic ring system, which is extended in length by the addition of a chlorine atom, appears to dictate an alternative mode of binding, which explains its nonselective binding to the F1*S and A variant hAGPs. Modeling experiments based on the co-crystal structures suggest that, in complexes of DSP, AMT, or CPZ with the F1*S variant, Phe-114 sterically hinders interactions with DSP and AMT, but not CPZ. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc

Pathogenic variation of Phakopsora pachyrhizi populations in Brazil.

The obligate basidiomycete Phakopsora pachyrhizi is the causal agent of soybean rust that has potential to reduce the yield of soybean drastically. Soybean production in Brazil has been threatened by the rust since the pathogen was first discovered in 2001. To understand pathogenic variation of the rust populations in South America, an evaluation system for soybean rust resistance has been constructed using a set of 16 differential varieties. In this study, the evaluation system was used to investigate pathogenic variation among the P. pachyrhizi populations in Brazil. Samples of P. pachyrhizi were collected from the diseased soybeans in Brazil in the 2007-2008 and 2008-2009 soybean cultivation seasons. In the first season, two rust samples showed similar pattern of the infection types on the differential set, suggesting that the same or similar pathogen population was present in the two locations. The other samples were likely different pathogenic populations. In the second season, different patterns of the infection types were found among the samples. Comparison of the evaluation data from the two seasons demonstrated that pathogenic variation between the seasons was detected in the populations from Rio Grande do Sul and Paraná but was not remarkable in those from Rondônia. This study provides useful knowledge about P. pachyrhizi populations in Brazil to identify the resistant soybean genotypes and target effective cultivars against certain pathogen populations.Edição do Proceedings of the National Soybean Rust Symposium, New Orleans, 2009

1 um Excess Sources in the UKIDSS - I. Three T Dwarfs in the SDSS Southern Equatorial Stripe

We report the discovery of two field brown dwarfs, ULAS J0128-0041 and ULAS J0321+0051, and the rediscovery of ULAS J0226+0051 (IfA 0230-Z1), in the Sloan Digital Sky Survey (SDSS) southern equatorial stripe. They are found in the course of our follow-up observation program of 1 um excess sources in the United Kingdom Infrared Telescope Infrared Deep Sky Survey. The Gemini Multi-Object Spectrographs spectra at red optical wavelengths (6500-10500 A) are presented, which reveal that they are early-T dwarfs. The classification is also supported by their optical to near-infrared colors. It is noted that ULAS J0321+0051 is one of the faintest currently known T dwarfs. The estimated distances to the three objects are 50-110 pc, thus they are among the most distant field T dwarfs known. Dense temporal coverage of the target fields achieved by the SDSS-II Supernova Survey allows us to perform a simple time-series analysis, which leads to the finding of significant proper motions of 150-290 mas/yr or the transverse velocities of 40-100 km/s for ULAS J0128-0041 and ULAS J0226+0051. We also find that there are no detectable, long-term (a-few-year) brightness variations above a few times 0.1 mag for the two brown dwarfs.Comment: Accepted for publication in the Astronomical Journal; Typos correcte

Microstructure and Structural Defects in MgB2 Superconductor

We report a detailed study of the microstructure and defects in sintered polycrystalline MgB2. Both TEM and x-ray data reveal that MgO is the major second-phase in our bulk samples. Although MgB2 and MgO have different crystal symmetries, being P6/mmm and Fm-3m, respectively, their stacking sequence of Mg and B (or O) and lattice spacings in certain crystallographic orientations are very similar. The size of MgO varies from 10~500nm, and its mismatch with the MgB2 matrix can be a source for dislocations. Dislocations in MgB2 often have a Burgers vector of . 1/3 and 1/3 partial dislocations and their associated stacking faults were also observed. Since both dislocations and stacking faults are located in the (001) basal plane, flux pinning anisotropy is expected. Diffuse scattering analysis suggests that the correlation length along the c-axis for defect-free basal planes is about 50nm. (001) twist grain-boundaries, formed by rotations along the c-axis, are major grain boundaries in MgB2 as a result of the out-of-plane weak bonding between Mg and B atoms. An excess of Mg was observed in some grain boundaries. High-resolution nano-probe EELS reveals that there is a difference in near edge structure of the boron K-edge acquired from grain boundaries and grain interiors. The change at the edge threshold may be suggestive of variation of the hole concentration that would significantly alter boundary superconductivity.Comment: 20 pages and 12 figure

Towards atomically precise manipulation of 2D nanostructures in the electron microscope

Despite decades of research, the ultimate goal of nanotechnology—top-down manipulation of individual atoms—has been directly achieved with only one technique: scanning probe microscopy. In this review, we demonstrate that scanning transmission electron microscopy (STEM) is emerging as an alternative method for the direct assembly of nanostructures, with possible applications in plasmonics, quantum technologies, and materials science. Atomically precise manipulation with STEM relies on recent advances in instrumentation that have enabled non-destructive atomicresolution imaging at lower electron energies. While momentum transfer from highly energetic electrons often leads to atom ejection, interesting dynamics can be induced when the transferable kinetic energies are comparable to bond strengths in the material. Operating in this regime, very recent experiments have revealed the potential for single-atom manipulation using the Ångströmsized electron beam. To truly enable control, however, it is vital to understand the relevant atomicscale phenomena through accurate dynamical simulations. Although excellent agreement between experiment and theory for the specific case of atomic displacements from graphene has been recently achieved using density functional theory molecular dynamics, in many other cases quantitative accuracy remains a challenge. We provide a comprehensive reanalysis of available experimental data on beam-driven dynamics in light of the state-of-the-art in simulations, and identify important targets for improvement. Overall, the modern electron microscope has great potential to become an atom-scale fabrication platform, especially for covalently bonded 2D nanostructures. We review the developments that have made this possible, argue that graphene is an ideal starting material, and assess the main challenges moving forward

Atomic Configuration of Nitrogen Doped Single-Walled Carbon Nanotubes

Having access to the chemical environment at the atomic level of a dopant in a nanostructure is crucial for the understanding of its properties. We have performed atomically-resolved electron energy-loss spectroscopy to detect individual nitrogen dopants in single-walled carbon nanotubes and compared with first principles calculations. We demonstrate that nitrogen doping occurs as single atoms in different bonding configurations: graphitic-like and pyrrolic-like substitutional nitrogen neighbouring local lattice distortion such as Stone-Thrower-Wales defects. The stability under the electron beam of these nanotubes has been studied in two extreme cases of nitrogen incorporation content and configuration. These findings provide key information for the applications of these nanostructures.Comment: 25 pages, 13 figure