PREVALÊNCIA DE ESPÉCIES DE Candida spp. ISOLADAS DA CAVIDADE ORAL E PRÓTESES DENTÁRIAS REMOVÍVEIS DE PACIENTES ATENDIDOS EM CLÍNICA ODONTOLÓGICA

O objetivo do presente trabalho foi determinar a prevalência de colonização e infecção por Candida spp. de mucosas e próteses de pacientes atendidos na clínica odontológica da Universidade do Oeste Paulista. Foram utilizados métodos morfológicos e bioquímicos para a identificação das espécies de Candida spp, tais como, CHROMagar Candida, o microcultivo em Cornmeal Agar, e o auxonograma. A prevalência de colonização por Candida spp. em usuários de próteses foi de 70,6%. Foram isoladas 101 amostras de Candida de 68 pacientes, das quais 69,31% foram identificadas como C. albicans e 30,69% como Candida não-albicans. A avaliação da prevalência de colonização da cavidade oral foi realizada em função do método de higienização da prótese e apresentou menor prevalência em pacientes que utilizavam a associação da escovação e molho no hipoclorito. A prevalência de Candida albicans foi maior que Candida não-albicans, e a higienização adequada da prótese reduziu a prevalência de colonização da cavidade oral

Metabolic, hormonal and immunological changes induced by acute intermittent exercise stages of training swimming

The effect of physical exercise in immune function has been extensively studied. The intensity and duration of physical exercise have considerable influence in immunologic parameters. However, few studies have compared different exercise intensities in different stages of a physical training program. To this point, we aimed to verify the metabolic, hormonal and immunologic changes before and after acute intermittent swimming exercise following different stages of training program. Seventeen male swimmers were analyzed in three stages of training, using the intensity of the three sessions was 90% (anaerobic potency – ANP), 70% (aerobic potency - AEP) and 98% (anaerobic ability – ANA) of the maximal speed from the best time of the distance, resulted from peak performance in competition. Blood samples were collected pre and immediately after exercise. Lactato increased significantly after the three different stages of training program, glucose post exercise in the ANP and AEP sessions, respectively. Glutamine increased significantly in AEP and ANA. Increased cortisol levels were also observed in AEP and in ANP. Leukocytes increased significantly after the three different sessions. There were no significant differences in lymphocytes, neutrophils e basophils. The eosinophils decreased in AEP and ANA. Monocytes no significant differences after the three different stages of training program, however decreased significantly on AEP. The immunoglobulins did not change of the three exercise patterns of pre and post-exercise. In summary, the protocoll intermittent swimming exercise following different stages of training the alterations capacity metabolic, hormones parameters and leukocytes any populations, favoring however health condition, so illnesses to not present.Vários estudos têm demonstrado a relação entre sistema imunológico e hormonal com o exercício físico e poucos estudos têm analisado esta relação em diferentes intensidades de esforço na natação. O objetivo desta pesquisa foi verificar as alterações metabólicas, hormonais e imunológicas antes e depois de um exercício de natação intermitente agudo com sessões diferentes durante o programa de treinamento. Dezessete nadadores do sexo masculino foram analisados em 3 sessões diferentes de treinamento, utilizando intensidades de 90% (potência anaeróbica – Pan), 70% (potência aeróbica – Pae) e 98% (capacidade anaeróbica – CAn) da velocidade máxima do melhor tempo de prova, resultado proveniente da melhor performance de competição. Amostras sanguíneas foram coletadas no pré e imediatamente após o exercício. Foi encontrado aumento significativo no lactato pós-exercício das três sessões de treinamento, na glicose pósexercício nas sessões de Pan e PAe, respectivamente. A glutamina aumentou significativamente no PAe e CAn. Foi observado um aumento nas concentrações de cortisol em PAe e em Pan. Os leucócitos aumentaram significativamente depois das três diferentes sessões. Não foram observadas diferenças significativas na concentração das imunoglobulinas e na contagem diferencial dos linfócitos, neutrófilos e basófilos. Os eosinófilos apresentaram diminuição significativa no pós-exercício de PAe e CAn em relação ao PAn e os monócitos não apresentaram alterações significativas no pósexercício nas três sessões, entretanto entre os tipos de treinamentos ocorreu uma diminuição significativa no PAe. Vários trabalhos revelam evidências das alterações nas concentrações e funções do sistema imune em decorrência do exercício físico. Esta apresentou protocolo de treinamento aplicado nos nadadores são adequados para alterar alguns componentes metabólicos, hormonais e leucocitários.TEDEBV UNIFESP: Teses e dissertaçõe

Evaluation of IgG and IgM anti-cytomegalovirus antibodies in parturient women and their newborns in the municipality of Presidente Prudente and region, State of São Paulo Pesquisa de anticorpos IgG e IgM para citomegalovírus em parturientes e recém-natos do município de Presidente Prudente e região, Estado de São Paulo

Primary infection by Cytomegalovirus (CMV) is usually followed by a persistent and/or recurrent infection. In most cases the infection is sub-clinical, but it can eventually be acute. This paper aims to evaluate by ELISA the frequency of IgG and IgM anti-CMV antibodies in 86 samples of parturient women and their newborns living in Presidente Prudente, SP, and vicinities. The samples were collected at Dr.Domingos Cerávolo University Hospital. Three parturient women (3.5%) were negative for anti-CMV antibodies, and 83(96.5%) were positive for IgG only. About the newborns, 83(96.5%) were positive for IgG only, 2(2.3%) where positive for both antibodies, and 1(1,2%) was negative for both antibodies. Results showed a high frequency of IgG anti-CMV antibodies in parturient women and their newborns. Moreover, we detected a relative high frequency of CMV congenitally infected newborns in this population, which might have an important impact on the amount of children with neurological and other diseases due to the virus infection<br>A infecção primária pelo citomegalovírus (CMV) é freqüentemente seguida por infecção persistente e/ou recorrente. Na grande maioria dos casos a infecção é subclínica, podendo ser aguda em certas condições. O objetivo deste trabalho foi determinar por ELISA a freqüência de anticorpos IgG e IgM para CMV, em 86 parturientes e seus recém-natos (RNs) provenientes do Hospital Universitário Dr. Domingos Cerávolo, Presidente Prudente, Estado de São Paulo. Os dados demonstraram que 83 parturientes (96,5%) apresentaram anticorpos IgG anti-CMV e 3(3,5%) não apresentaram anticorpos anti-CMV. Quanto aos RNs, 1(1,2%) não apresentou anticorpos anti-CMV, 83(96,5%) apresentaram anticorpos IgG e 2(2,3%) apresentaram anticorpos IgM, caracterizando infecção aguda. Verificamos, assim, uma alta freqüência de anticorpos IgG para CMV em gestantes e RNs, bem como uma relativa alta freqüência de RNs infectados congenitamente pelo CMV nessa população, o que pode ter um impacto importante no número de crianças com problemas neurológicos e outros decorrentes da infecção por esse víru

Differential arousal of state anxiety and problem solving performance

Master of EducationIn Part A of an experiment, tertiary students were assigned at random to either failure or success experiences with three types of task materials. The effects of manipulations on reported state anxiety levels were examined using pro-treatment levels as the covariate. Data from ten replications indicated that, although adjusted means of failure groups were significantly higher than those of success groups, the magnitude of anxiety arousal depended upon the material used in the experimental treatment. Results indicated that exposure to anagram materials produced more marked variation in anxiety levels than either number or vocabulary materials. It was clear that students experiencing stronger failure showed higher adjusted state anxiety scores than did students experiencing milder failure or success. Greater lability of scores was found for female subjects and state anxiety arousal was found to be correlated significantly with proneness to test anxiety. In Part B, which was quasi-experimental, all subjects worked through a set of letter series problems. In post-hoc analyses, subjects within the failure or success treatments were classified by degree of proneness to test anxiety and level of orientation towards achievement. Levels of test anxiety and aroused state anxiety were associated with differences in both number of problems attempted and number of items abandoned. A significant triple interaction on the log time spent correctly solving problems, suggested that high state anxiety could either facilitate or debilitate performance depending on the strength of the trait differences. These results were consistent with the hypothesised operation of either impulsive or reflective strategies activated to cope with high anxiety arousal. It was found that reversed items of the State Anxiety Scale tended to reflect increases in situational stress to a greater extent than did directly stated items. The practice of reversing the scoring weights on reversed items to derive state anxiety scores does not appear to produce an exact alignment with the meaning of the directly stated anxiety items

Taeniosis-cysticercosis complex in individuals of a peasants’ settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

In order to evaluate the taeniosis-cysticercosis complex in a population of a peasants' settlement, located at Teodoro Sampaio, state of São Paulo, Brazil (longitude 52°36'12", latitude 22°17'12") a series of laboratory markers were determined. After signing an informed consent, participants answered a standardized questionnaire. To determine anti-Taenia solium cysticercus antibodies, the samples were tested by enzyme linked immunoabsorbent assay using 18-and 14-kDa antigen proteins from vesicular fluid of Taenia crassiceps (VF-Tcra). The reactive and inconclusive ELISA samples were tested by immunoblotting. Total IgE levels were determined by chemmiluminescence's assay and hemogram by flow cytometer flux counter. A total of 84 individuals, 5.9% presented anti-T. solium cysticercus antibodies in ELISA and 3.6% were strongly reactive in the 18/14 kDa immunoblotting confirmatory test. All of the individuals with positive antibodies showed elevated Total IgE levels. We conclude that the frequency of anti-T. solium cysticercus antibodies in this population is higher than other regions considered endemic in São Paulo. Thus, it is important to carry out surveys in Peasants' settlement areas with the objective of establishing public health measures for prevention and control of infectious diseases such as taeniosis-cysticercosis