Faster HIV-1 Disease Progression among Brazilian Individuals Recently Infected with CXCR4-Utilizing Strains

Introduction: Primary HIV infection is usually caused by R5 viruses, and there is an association between the emergence of CCXR4-utilizing strains and faster disease progression. We characterized HIV-1 from a cohort of recently infected individuals in Brazil, predicted the virus's co-receptor use based on the env genotype and attempted to correlate virus profiles with disease progression. Methods: A total of 72 recently infected HIV patients were recruited based on the Serologic Testing Algorithm for Recent HIV Seroconversion and were followed every three to four months for up to 78 weeks. The HIV-1 V3 region was characterized by sequencing nine to twelve weeks after enrollment. Disease progression was characterized by CD4+ T-cell count decline to levels consistently below 350 cells/mu L. Results: Twelve out of 72 individuals (17%) were predicted to harbor CXCR4-utilizing strains; a baseline CD4,350 was more frequent among these individuals (p = 0.03). Fifty-seven individuals that were predicted to have CCR5-utilizing viruses and 10 individuals having CXCR4-utilizing strains presented with baseline CD4.350; after 78 weeks, 33 individuals with CCR5 strains and one individual with CXCR4 strains had CD4.350 (p = 0.001). There was no association between CD4 decline and demographic characteristics or HIV-1 subtype. Conclusions: Our findings confirm the presence of strains with higher in vitro pathogenicity during early HIV infection, suggesting that even among recently infected individuals, rapid progression may be a consequence of the early emergence of CXCR4-utilizing strains. Characterizing the HIV-1 V3 region by sequencing may be useful in predicting disease progression and guiding treatment initiation decisions.Brazilian Program for STD and AIDSBrazilian Program for STD and AIDSMinistry of Health [914/BRA/3014-UNESCO/Kallas]Ministry of HealthSao Paulo City Health DepartmentSao Paulo City Health Department [2004-0.168.922-7/Kallas]Fundacao de Amparo a Pesquisa do Estado de Sao PauloFundacao de Amparo a Pesquisa do Estado de Sao Paulo [04/15856-9/Diaz]Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)Brazilian Ministry of EducationBrazilian Ministry of Educatio

Elevated Risk for HIV-1 Infection in Adolescents and Young Adults in São Paulo, Brazil

Background. Recent studies have sought to describe HIV infection and transmission characteristics around the world. Identification of early HIV-1 infection is essential to proper surveillance and description of regional transmission trends. In this study we compare people recently infected (RI) with HIV-1, as defined by Serologic Testing Algorithm for Recent HIV Seroconversion (STARHS), to those with chronic infection. Methodology/Principal Findings Subjects were identified from 2002-2004 at four testing sites in São Paulo. Of 485 HIV-1-positive subjects, 57 (12%) were defined as RI. Of the participants, 165 (34.0%) were aware of their serostatus at the time of HIV-1 testing. This proportion was statistically larger (p<0.001) among the individuals without recent infection (n = 158, 95.8%) compared to 7 individuals (4.2%) with recently acquired HIV-1 infection. In the univariate analysis, RI was more frequent in <25 and >59 years-old age strata (p<0.001). The majority of study participants were male (78.4%), 25 to 45 years-old (65.8%), white (63.2%), single (61.7%), with family income of four or more times the minimum wage (41.0%), but with an equally distributed educational level. Of those individuals infected with HIV-1, the predominant route of infection was sexual contact (89.4%), with both hetero (47.5%) and homosexual (34.5%) exposure. Regarding sexual activity in these individuals, 43.9% reported possible HIV-1 exposure through a seropositive partner, and 49.4% reported multiple partners, with 47% having 2 to 10 partners and 37.4% 11 or more; 53.4% of infected individuals reported condom use sometimes; 34.2% reported non-injecting, recreational drug use and 23.6% were reactive for syphilis by VDRL. Subjects younger than 25 years of age were most vulnerable according to the multivariate analysis. ) Conclusions/Significance In this study, we evaluated RI individuals and discovered that HIV-1 has been spreading among younger individuals in São Paulo and preventive approaches should, therefore, target this age stratu

Antiretroviral resistance in individuals presenting therapeutic failure and subtypes of the human immunodeficiency virus type 1 in the Northeast Region of Brazil

This study aimed to analyze human immunodeficiency virus (HIV) mutation profiles related to antiretroviral resistance following therapeutic failure, and the distribution of hiv subtypes in the Northeast Region of Brazil. A total of 576 blood samples from AIDS patients presenting therapeutic failure between 2002 and 2004 were analyzed. The genotyping kit viroSeq® was used to perform viral amplification in order to identify mutations related to hiv pol gene resistance. An index of 91.1% of the patients presented mutations for nucleoside reverse transcriptase inhibitors (nrti), 58.7% for non-nucleoside reverse transcriptase inhibitors (nnrti), and 94.8% for protease inhibitors (pi). The most prevalent mutations were 184V and 215E for nrti, 103N and 190A for nnrti. Most mutations associated with PIs were secondary, but significant frequencies were observed in codons 90 (25.2%), 82 (21.1%), and 30 (16.2%). The resistance index to one class of antiretrovirals was 14%, to two classes of antiretrovirals 61%, and to three classes 18.9%. Subtype B was the most prevalent (82.4%) followed by subtype F (11.8%). The prevalence of mutations related to nrti and nnrti was the same in the two subtypes, but codon analysis related to PI showed a higher frequency of mutations in codon 63 in subtype B and in codon 36 in subtype F. The present study showed that there was a high frequency of primary mutations, which offered resistance to nrti and nnrti. Monitoring patients with treatment failure is an important tool for aiding physicians in rescue therapy

Morbidade referida e seus condicionantes em crianças de 5 a 9 anos em Sobral, CE, Brasil Reported morbidity and its conditionings in children 5 to 9 years old in Sobral, CE, Brazil

OBJETIVO: Descrever a morbidade referida em crianças de 5 a 9 anos e analisar seus possíveis condicionantes. METODOLOGIA: Corte de base populacional com amostra aleatória e representativa em crianças de 5 a 9 anos da zona urbana de Sobral - CE, Brasil. Entrevistas domiciliares com 3.276 crianças e exame clínico em 2.594. A morbidade referida foi classificada segundo a CID-10. O desfecho analisado foi morbidade referida nos últimos 15 dias, utilizando Stata 7.0. RESULTADOS: 43,9% das crianças apresentaram morbidade referida: Doenças do Aparelho Respiratório (DAR), 28,7%; doenças da pele, 3,4%; doenças infecciosas, 2,2%. Daquelas que adoeceram, 41,5% procuraram atendimento. Dessas, 77,4% em Unidades de Saúde Familiar. Apresentaram maiores chances de DAR as crianças com as seguintes características: residir no núcleo urbano principal do município, ter antecedente de desnutrição e frequentar escola, com um valor de ODDS Ratio, respectivamente de 1,48 (IC95%1,10-1,99), 1,30 (IC95%1,05-1,60) e 1,54 (IC95%1,02-2,32). DISCUSSÃO: Chamou a atenção a elevada prevalência de DAR em crianças do núcleo urbano principal, cerca de duas vezes maior que a observada em outros estudos de metodologia similar, levantando hipóteses de sobrerelato ou poluição ambiental. A maioria das crianças apresentou problemas de saúde de menor gravidade e teve acesso facilitado aos serviços de saúde, principalmente ao PSF. CONCLUSÕES: Serão necessários novos estudos para identificar possíveis causas da elevada prevalência de DAR em crianças do núcleo urbano principal do município. As ocasiões em que as crianças demandam os serviços de saúde da família por doenças de baixa gravidade podem ser aproveitadas para medidas de prevenção e promoção da saúde.<br>INTRODUCTION: This study was based on a random sample of 3,276 children aged between 5 and 9 years from the urban region of Sobral - CE, Brazil. OBJECTIVE: The main goal was to describe reported morbidity in children aged between 5 to 9 years and to analyze their conditionings. METHODOLOGY: Home interviews were carried out with 3,276 children and clinical exams were conducted in a sub-sample of 2,594. Health problems reported by mothers were classified according to the tenth revision of the International Disease Classification. Analyses were performed using Stata 7.0. Outcomes analyzed were reported morbidity in the past 15 days. The independent variables were grouped into socioeconomic, environmental, health service access, and nutritional status. RESULTS: The most prevalent morbidities were diseases of the respiratory system (DRS), 28.7%; diseases of the skin and subcutaneous tissue, 3.4%; infectious and parasitic diseases, 2.2%. The independent variables that had statistically significant correlations with the prevalence of DRS were related to the children who lived in the main urban center, had a history of malnutrition, and low school attendance, with odds ratio of respectively, 1.48 (1.10-1.9995%CI), 1.30 (1.05-1.6095%CI), 1.54 (1.02-2.3295%CI). DISCUSSION: A high prevalence of DRS was observed in children in the main urban center. DRS prevalence was about twice higher than that observed in other studies with similar methodology. This observation has raised the hypotheses of over reporting or environmental pollution. Most children had minor health problems. They also had facilitated access to health services, especially to the FHP

Neurocognitive impairment in HIV-1 clade C- versus B-infected individuals in Southern Brazil

HIV-1 clade C isolates show reduced Tat protein chemoattractant activity compared with clade B. This might influence neuropathogenesis by altering trafficking of monocytes into the CNS. A previous study suggested low rates of HIV-associated dementia in clade C infected individuals. The present study evaluated neurocognitive impairment rates in clade B- and C-infected individuals from the same local population. HIV+ and HIV- participants were recruited from the same geographic region in southern Brazil. We evaluated neuropsychological (NP) impairment using a screening instrument (the International HIV Dementia Scale; IHDS), as well as a Brazilian Portuguese adaptation of a comprehensive battery that has demonstrated sensitivity to HIV associated neurocognitive disorders (HAND) internationally. NP performance in controls was used to generate T-scores and impairment ratings by the global deficit score (GDS) method. Clade assignments were ascertained by sequencing pol and env. Blood and cerebrospinal fluid (CSF) were collected from all HIV+ participants. HIV+ and HIV- participants were comparable on demographic characteristics. HIV+ participants overall were more likely to be impaired than HIV- by the IHDS and the GDS. Clade B and C infected individuals were demographically similar and did not differ significantly in rates of impairment. The prevalence of pleocytosis, a marker of intrathecal cellular chemotaxis, also did not differ between clade B and C infections. Clade B and C HIV-infected individuals from the same geographic region, when ascertained using comparable methods, did not differ in their rates of neurocognitive impairment, and there was no evidence of differences in CNS chemotaxis