A Generational Approach to Modelling Youth’s Engagement in Politics

Youth in contemporary Anglo-Saxon democracies are less engaged in politics than their adult counterparts. To explain why, this study adopts a generational approach to modelling youth’s engagement in politics. Contemporary youth are characterised by progressive individualist values of democratisation and individualism that respond to the instability and uncertainty of late modernity. Linear regressions are used on datasets extracted from the World Values Survey for four study countries – Australia, the USA, Britain and New Zealand. The results show that the conventional civic engagement model no longer explains why contemporary youth are statistically less engaged in politics than adults. The index of progressive individualism developed in this study, however, does diminish the impact of age in determining variation in political engagement. Although further research is needed to confirm progressive individualism is a generational characteristic of contemporary youth, this study advocates for increased flexibility in the practice of politics to reflect the lived experience of young citizens in late modernity

Profiling of the secretome of human cancer cells: Preparation of supernatant for proteomic analysis

Secretomic analysis requires removal of serum proteins from cell-culture media. We evaluate the proteins washed from cells prepared in bovine serum-supplemented medium. PBS and serum-free-medium (SFM) were the washing solutions. A Bradford assay was used for total protein concentration and a 1D gel and LC-MS/MS, to assign the protein to human or bovine origin. For both wash solutions, all bovine protein had been removed by the third wash, without compromising the number of living cells. Further washes reduced the number of living cells, especially when using PBS. Proteomic analysis of wash supernatant showed that SFM induced greater lysis of dead cells. Three washes were sufficient to minimize the effects on cell viability, while still removing serum proteins. Washing in SFM resulted in contamination of the wash supernatant with lysed dead cell proteins. Washed cells were incubated in SFM and exposed to ionizing radiation. Analysis of the supernatant showed an increase in human cytoplasmic, plasma membrane, and nuclear protein following irradiation. Secreted proteins were also detected, but in smaller quantities. The significance of these findings extend to in vitro studies of bystander phenomena, since the proteins of lysed dead cells may participate in driving bystander responses

Analysis of variation in calibration curves for Kodak XV radiographic film using modelâ based parameters

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135597/1/acm20222.pd

Limonite – a weathered residual soil heterogeneous at all scales

Limonite is a residual soil produced by the decomposition of magnesium silicate (olivine) rocks in tropical environments. During weathering most of the original rock is leached away leaving only its iron content, which is precipitated out in the form of iron sesqui-oxides to create a soft and highly porous soil. The predominant mineral present in limonite is goethite, which forms acicular nanoparticles that agglomerate to produce a silty sand with porous particles. The void ratio varies from 2 to 6, with higher values being a consequence of structure-supported voids. An extensive set of laboratory tests have been performed on a limonite soil profile which extends 50 m to rock. These data show that there is no pattern to shear strength with depth, with the shear strength equally likely to be 50 or 200 kPa through much of the profile. It is argued that the shear strength parameters for failure mechanisms, having any significant length, should be based on average values. The letter presents scanning electron microscopy photographs showing the fundamental particles, the results of triaxial tests comparing natural and reconstituted behaviour which show the effects of microstructure on the meso-scale response, and field data to show site variability

Organisational structures and processes for health and well-being: insights from work integration social enterprise

Background: Previous research on employee well-being for those who have experienced social and economic disadvantage and those with previous or existing mental health conditions has focused mainly on programmatic interventions. The purpose of this research was to examine how organisational structures and processes (such as policies and culture) influence well-being of employees from these types of backgrounds. Methods: A case study ethnographic approach which included in-depth qualitative analysis of 93 semi-structured interviews of employees, staff, and managers, together with participant observation of four social enterprises employing young people. Results: The data revealed that young people were provided a combination of training, varied work tasks, psychosocial support, and encouragement to cultivate relationships among peers and management staff. This was enabled through the following elements: structure and space; funding, finance and industry orientation; organisational culture; policy and process; and fostering local service networks. The findings further illustrate how organisational structures at these workplaces promoted an inclusive workplace environment in which participants self-reported a decrease in anxiety and depression, increased self-esteem, increased self-confidence and increased physical activity. Conclusions: Replicating these types of organisational structures, processes, and culture requires consideration of complex systems perspectives on implementation fidelity which has implications for policy, practice and future research

Lessons from Tau-Deficient Mice

Both Alzheimer's disease (AD) and frontotemporal dementia (FTD) are characterized by the deposition of hyperphosphorylated forms of the microtubule-associated protein tau in neurons and/or glia. This unifying pathology led to the umbrella term “tauopathies” for these conditions, also emphasizing the central role of tau in AD and FTD. Generation of transgenic mouse models expressing human tau in the brain has contributed to the understanding of the pathomechanistic role of tau in disease. To reveal the physiological functions of tau in vivo, several knockout mouse strains with deletion of the tau-encoding MAPT gene have been established over the past decade, using different gene targeting constructs. Surprisingly, when initially introduced tau knockout mice presented with no overt phenotype or malformations. The number of publications using tau knockout mice has recently markedly increased, and both behavioural changes and motor deficits have been identified in aged mice of certain strains. Moreover, tau knockout mice have been instrumental in identifying novel functions of tau, both in cultured neurons and in vivo. Importantly, tau knockout mice have significantly contributed to the understanding of the pathophysiological interplay between Aβ and tau in AD. Here, we review the literature that involves tau knockout mice to summarize what we have learned so far from depleting tau in vivo

Exploring value creation from an ecosystem perspective: A critical examination of social procurement policy

From qualitative research undertaken in Scotland, the authors analyse procurement policy reforms. Utilizing a public service ecosystem framework, barriers to value creation were found, including overly bureaucratic practices, centrally devised rules, and conflicting organizational aims. The authors develop insights into ways policy design and implementation creates (or does not create) value across the many levels of an ecosystem where procurement policy reforms have impact, demonstrating the benefits of using a service ecosystem framework for understanding nuanced impacts and perceptions of policy implementation

Exploring value creation from an ecosystem perspective: A critical examination of social procurement policy

From qualitative research undertaken in Scotland, the authors analyse procurement policy reforms. Utilizing a public service ecosystem framework, barriers to value creation were found, including overly bureaucratic practices, centrally devised rules, and conflicting organizational aims. The authors develop insights into ways policy design and implementation creates (or does not create) value across the many levels of an ecosystem where procurement policy reforms have impact, demonstrating the benefits of using a service ecosystem framework for understanding nuanced impacts and perceptions of policy implementation

Single Step Plasma Process for Covalent Binding of Antimicrobial Peptides on Catheters to Suppress Bacterial Adhesion

Catheter-associated biofilms are responsible for a large fraction of hospital acquired infections. Antimicrobial surface coating on catheters providing prevention at source is extensively studied to reduce bacterial adhesion. Antimicrobial peptides such as melimine and Mel4, covalently linked to surfaces have shown excellent potential in animal and human studies to suppress infection without toxicity. Covalent binding of the peptides on catheter surfaces improves efficacy but so far has been implemented using multi-step wet chemical coupling that will impede widespread adoption. Here we demonstrate plasma immersion ion implantation (PIII) as a single step treatment that covalently couples antimicrobial peptides to polyvinyl chloride (PVC). Strong antimicrobial activity was demonstrated by higher than 3 log kill of S. aureus. A variant of the process was demonstrated as an antimicrobial treatment for chemically inert glass surfaces. Covalent coupling was rigorously tested by stringent SDS washing. We further demonstrated that the plasma treatment can effectively functionalize both internal and external surfaces of catheter tubing, reducing 99% of bacterial adhesion. The process is feasible as a patient-safe treatment for treating various types of catheters and is suitable for commercial mass production. In a logical extension of the work, the process could be adapted to bone replacement scaffolds of all types including metallic, polymeric and ceramic