Feto-maternal outcome in previous one cesarean section: a retrospective observational study at a district hospital of Jharkhand

Background: Cesarean section is one of the most common surgery done in department of obstetrics and gynecology. It becomes more complicated in cases of high risk pregnancy like PIH, APH etc. Its rate is increasing day by day. Once a CS, always a CS, is questionable but being followed up at various set up due to various reasons. Today, one of the important indications of repeat CS is previous CS which increases the rate of CS in a particular set up and morbidities associated with multiple CS. According to ACOG guidelines, VBAC should be attempted to decrease the incidence of repeat CS and morbidity among them. This study was conducted to analyze the incidence of subsequent CS in previous one CS cases, demographic variables and feto-maternal outcome in previous CS patients.Methods: This was a retrospective observational study on previous CS patients, carried out in District hospital, Dumka, Jharkhand. The rates, demography and feto-maternal outcome among previous one CS patients were studied during 1 year duration and data was obtained from labor room and medical record department.Results: Out of 2947 deliveries, 63 patients had previous one cesarean section, the incidence being 2.13%. 39.7% deliver vaginally while 60.3% needed repeat cesarean section. Majority (95.2%) were in 21-30yrs age group, 76.2% were unbooked and 25.4% were tribal population. Maximum (79.4%) were gravid 2 and 93.7% were term. 93.7% had birth space >18 months. Out of 38 repeat cesarean section, 78.9% underwent emergency cesarean section. The most common indication being scar tenderness (39.5%) followed by CPD (15.8%). Intraoperatively, severe adhesions found in 15.8% cases, bladder adherence in 18.4% cases and 2.6% had adherent placenta. PPH occurred in 4.8% cases and blood transfusion or injectable iron therapy required in 25.4% cases. 4.7% had wound infection, 7.9% had prolong hospital stay and 22.2% cases required prolong foley’s catheterization. There was no maternal mortality. 95% babies born alive, 3.2% was stillbirth, 1.5% was IUD and the neonatal death rate was 3.2%.Conclusions: Previous cesarean section is one of the most important causes of CS in subsequent pregnancies, hence increase in the rates of CS and the morbidities associated with multiple CS among mothers. Thus the decision of CS in primigravida should be taken wisely and practice of CS on demand should be discouraged.

Prevalence of hepatitis B in pregnant women and management of babies born to Hepatitis B positive mother: A criterion based clinical audit

Introductions: This study aims at identifying the prevalence of Hepatitis B virus infection in pregnant women and adherence to the management of the newborns of these women as per the international guidelines. Methods: This is a cross sectional study conducted at Patan Hospital from January 1, 2008 to December 30, 2014. Prevalence of HBsAg in pregnant women and management of babies born to these women, immunization status, breast feeding status and follow up advise for the babies were studied.  These data were analyzed to see whether we were following international guidelines for preventing mother to child transmission of hepatitis B infection. Results: There were total of 58,917 deliveries and 59,438 births during the seven year study period. Total 148 cases of pregnant ladies with HBsAg positive status were recorded. Prevalence of HBsAg positive case was 1 in every 398 (0.25%) deliveries. Fifty four babies (87%) received hepatitis B vaccine (HBV) and hepatitis B immunoglobulin (HBIG) within 12 hours of birth. Eight babies (22%) failed to receive HBV and HBIG within 12hours of birth. Mother’s HBsAg positive status was not the reason for formula feeding in any of the babies. Follow up HBsAg status was not advised in any of the babies. Conclusions: At Patan hospital, we seem to be following most of the guidelines in the management of HBV infection, except for checking the HBsAg status of the babies at 9-18 months of age.Keywords: chronic liver disease, HBsAg positive, hepatitis B vaccine, Hepatitis B immunoglobulin, mother to child transferÂ

Premenstrual syndrome and its association with menstrual profile among female students of colleges in Ujjain city, Madhya Pradesh, India

Background: Premenstrual Syndrome (PMS) is a common psychosomatic disorder which affects 30%-50% of women in child bearing age. Symptoms’ have devastating effect which starts early in life. So, the study was conducted to estimate the prevalence of premenstrual syndrome (PMS) and its association with menstrual profile among female students of colleges situated in Ujjain city.Methods: Observational follow-up study was conducted at 4 colleges selected by convenience sampling located in Ujjain city. PMS and menstrual profile related data from 250 girls (18-25years) who consented to participate were collected using a modified version of daily record of severity of problems for atleast 2 consecutive menstrual cycles. Diagnosis of PMS was made using ACOG criteria. Data analysis was done using SPSS version 16.0.Results: The prevalence of PMS was 39.6% and was found to be negatively associated with any history of mothers having menstrual problems.Conclusions: Frequency of PMS is relatively common in young girls and has negative association with any history of mothers having menstrual problems. Measures should be adopted so that mothers can condition their daughters about premenstrual symptoms which will influence the quality of life of young females

Study to assess the association of PMS with sociodemographic factors and various coping behaviors adopted for premenstrual symptoms

Background: Some women get through their monthly periods easily with few or no concerns. However, other women experience a host of physical and/or emotional symptoms just before and during menstruation (heavy bleeding and missed periods to unmanageable mood swings). These premenstrual syndrome (PMS) symptoms usually start to be problematic in the adolescent years and decline in the climacteric. Symptoms have a devastating effect which starts early in life. Aims and Objectives: The study was conducted to estimate the prevalence of PMS and its association with sociodemographic factors among female students of colleges situated in Ujjain city and coping behavior adopted to alleviate these symptoms. Materials and Methods: An observational follow-up study was conducted at 4 colleges selected by convenience sampling located in Ujjain city. PMS and menstrual profile-related data from 250 girls (18–25 years) who consented to participate were collected using a modified version of the Daily Record of Severity of Problems for at least 2 consecutive menstrual cycles. The diagnosis of PMS was made using the American College of Obstetrics and Gynecology criteria. Data analysis was done using SPSS version 16.0. Results: The prevalence of PMS was 39.6% and was not associated with any of the sociodemographic factors. It was found that majority of participants turned to healthy way of dealing with symptoms, i.e., taking hot or cold beverages and talking to family members. Conclusion: The frequency of PMS is relatively common in young girls, and most of the girls adopt home remedies to alleviate symptom

Altered expression and editing of miRNA-100 regulates iTreg differentiation

RNA editing ofmiRNAs, especially in the seed region, adds another layer to miRNA mediated gene regulation which can modify its targets, altering cellular signaling involved in important processes such as differentiation. In this study, we have explored the role of miRNA editing in CD4+ T cell differentiation. CD4+ T cells are an integral component of the adaptive immune system. Na¨ıve CD4+ T cells, on encountering an antigen, get differentiated either into inflammatory subtypes like Th1, Th2 or Th17, or into immunosuppressive subtype Treg, depending on the cytokine milieu. We found C-to-U editing at fifth position of mature miR-100, specifically in Treg. The C-to-U editing of miR-100 is functionally associated with at least one biologically relevant target change, from MTOR to SMAD2. Treg cell polarization by TGFβ1 was reduced by both edited and unedited miR-100 mimics, but percentage of Treg in PBMCs was only reduced by edited miR-100 mimics, suggesting a model in which de-repression of MTOR due to loss of unedited mir-100, promotes tolerogenic signaling, while gain of edited miR-100 represses SMAD2, thereby limiting the Treg. Such delicately counterbalanced systems are a hallmark of immune plasticity and we propose that miR-100 editing is a novel mechanism toward this end

Clinico-laboratory profile of dengue patients in a tertiary hospital of Eastern India

Background: Dengue is the most rapidly spreading mosquito-borne viral disease of mankind. According to WHO, about 50-100 million new dengue infections are estimated to occur annually in more than 100 endemic countries.So, the present study has been done to analyzed varied clinical and laboratory profile of confirm dengue cases.Methods: Cross-sectional observational study was undertaken among 106 adult Ig M Ab positive cases admitted during October 2015 to September 2017. All patients were evaluated clinically and subjected for relevant laboratory investigations.Results: In our study male and female ratio was 20.2:1. Most common symptom was fever (95.3%) and least common was jaundice. Second most symptom was headache (70.8%). Myalgia was present in 49.1% of cases. 54.7% had nausea/vomiting. Hypotension was noticed in 33% of cases whereas encephalopathy and melaena was found in 6.6% and 2.83% respectively. Leucopenia was present in 57.5% and thrombocytopenia in 50.9% of cases.Conclusions: Younger male were commonly affected with dengue fever and promptly responded to conservative therapy due to early confirmation of diagnosis. Mortality was only 1.8% cases, may be due to delay in seeking medical attention

Ablation of RNA interference and retrotransposons accompany acquisition and evolution of transposases to heterochromatin protein CENPB

Inactivation of retrotransposons is accompanied by the emergence of centromere-binding protein-B (CENPB) in Schizosaccharomyces, as well as in metazoans. The RNA interference (RNAi)-induced transcriptional silencing (RITS) complex, comprising chromodomain protein-1 (Chp1), Tas3 (protein with unknown function), and Argonaute (Ago1), plays an important role in RNAi-mediated heterochromatinization. We find that whereas the Ago1 subunit of the RITS complex is highly conserved, Tas3 is lost and Chp1 is truncated in Schizosaccharomyces cryophilus and Schizosaccharomyces octosporus. We show that truncated Chp1 loses the property of heterochromatin localization and silencing when transformed in Schizosaccharomyces pombe. Furthermore, multiple copies of CENPB, related to Tc1/mariner and Tc5 transposons, occur in all Schizosaccharomyces species, as well as in humans, but with loss of transposase function (except Schizosaccharomyces japonicus). We propose that acquisition of Tc1/mariner and Tc5 elements by horizontal transfer in S. pombe (and humans) is accompanied by alteration of their function from a transposase/endonuclease to a heterochromatin protein, designed to suppress transposon expression and recombination. The resulting redundancy of RITS may have eased the selection pressure, resulting in progressive loss or truncation of tas3 and chp1 genes in S. octosporus and S. cryophilus and triggered similar evolutionary dynamics in the metazoan orthologues

Antibacterial, Antifungal and Antiviral Nanocomposites: Recent Advances and Mechanisms of Action

Over the past ten years, there has been a significant increase in research into the study of nanocomposites. Nanocomposites vary in their physical and chemical properties. In today’s era, eco-friendly, nontoxic, biocompatible, biobased fillers and composites should be synthesized to increase their societal value in various aspects. These materials have seen extensive use across several industries, from biosensors to biomedicine. Great strides have been made in the field of Microbiology, particularly as Antibacterial agents, among these applications. The objective of this review is to present a thorough analysis of several Nanocomposites that reveal promising antibacterial activity. Such Nanocomposites are reviewed in detail, as well as their antibacterial efficacy is discussed

Reinterpretation of LHC Results for New Physics: Status and recommendations after Run 2

We report on the status of efforts to improve the reinterpretation of searches and measurements at the LHC in terms of models for new physics, in the context of the LHC Reinterpretation Forum. We detail current experimental offerings in direct searches for new particles, measurements, technical implementations and Open Data, and provide a set of recommendations for further improving the presentation of LHC results in order to better enable reinterpretation in the future. We also provide a brief description of existing software reinterpretation frameworks and recent global analyses of new physics that make use of the current data

Report from Working Group 3: Beyond the standard model physics at the HL-LHC and HE-LHC

This is the third out of five chapters of the final report [1] of the Workshop on Physics at HL-LHC, and perspectives on HE-LHC [2]. It is devoted to the study of the potential, in the search for Beyond the Standard Model (BSM) physics, of the High Luminosity (HL) phase of the LHC, defined as $3$ ab$^{-1}$ of data taken at a centre-of-mass energy of 14 TeV, and of a possible future upgrade, the High Energy (HE) LHC, defined as $15$ ab$^{-1}$ of data at a centre-of-mass energy of 27 TeV. We consider a large variety of new physics models, both in a simplified model fashion and in a more model-dependent one. A long list of contributions from the theory and experimental (ATLAS, CMS, LHCb) communities have been collected and merged together to give a complete, wide, and consistent view of future prospects for BSM physics at the considered colliders. On top of the usual standard candles, such as supersymmetric simplified models and resonances, considered for the evaluation of future collider potentials, this report contains results on dark matter and dark sectors, long lived particles, leptoquarks, sterile neutrinos, axion-like particles, heavy scalars, vector-like quarks, and more. Particular attention is placed, especially in the study of the HL-LHC prospects, to the detector upgrades, the assessment of the future systematic uncertainties, and new experimental techniques. The general conclusion is that the HL-LHC, on top of allowing to extend the present LHC mass and coupling reach by $20-50\%$ on most new physics scenarios, will also be able to constrain, and potentially discover, new physics that is presently unconstrained. Moreover, compared to the HL-LHC, the reach in most observables will, generally more than double at the HE-LHC, which may represent a good candidate future facility for a final test of TeV-scale new physics