Shining light on the function of NPH3/RPT2-like proteins in phototropin signalling

No abstract available

Investigation of varying gray scale levels for remote manipulation

A study was conducted to investigate the effects of variant monitor gray scale levels and workplace illumination levels on operators' ability to discriminate between different colors on a monochrome monitor. It was determined that 8-gray scale viewing resulted in significantly worse discrimination performance compared to 16- and 32-gray scale viewing and that there was only a negligible difference found between 16 and 32 shades of gray. Therefore, it is recommended that monitors used while performing remote manipulation tasks have 16 or above shades of gray since this evaluation has found levels lower than this to be unacceptable for color discrimination task. There was no significant performance difference found between a high and a low workplace illumination condition. Further analysis was conducted to determine which specific combinations of colors can be used in conjunction with each other to ensure errorfree color coding/brightness discrimination performance while viewing a monochrome monitor. It was found that 92 three-color combination and 9 four-color combinations could be used with 100 percent accuracy. The results can help to determine which gray scale levels should be provided on monochrome monitors as well as which colors to use to ensure the maximal performance of remotely-viewed color discrimination/coding tasks

Single-trial multiwavelet coherence in application to neurophysiological time series

A method of single-trial coherence analysis is presented, through the application of continuous muldwavelets. Multiwavelets allow the construction of spectra and bivariate statistics such as coherence within single trials. Spectral estimates are made consistent through optimal time-frequency localization and smoothing. The use of multiwavelets is considered along with an alternative single-trial method prevalent in the literature, with the focus being on statistical, interpretive and computational aspects. The multiwavelet approach is shown to possess many desirable properties, including optimal conditioning, statistical descriptions and computational efficiency. The methods. are then applied to bivariate surrogate and neurophysiological data for calibration and comparative study. Neurophysiological data were recorded intracellularly from two spinal motoneurones innervating the posterior,biceps muscle during fictive locomotion in the decerebrated cat

Functional characterization of a constitutively active kinase variant of Arabidopsis phototropin 1

Phototropins (phots) are plasma membrane-associated serine/threonine kinases that coordinate a range of processes linked to optimizing photosynthetic efficiency in plants. These photoreceptors contain two light-, oxygen- or voltage-sensing (LOV) domains within their N-terminus, with each binding one molecule of flavin mononucleotide (FMN) as a UV/blue light absorbing chromophore. Although phots contain two LOV domains, light-induced activation of the C-terminal kinase domain and subsequent receptor autophosphorylation is controlled primarily by the A′α-LOV2-Jα photosensory module. Mutations that disrupt interactions between the LOV2-core and its flanking helical segments can uncouple this mode of light regulation. Yet, the impact of these mutations on phot function in Arabidopsis has not been explored. Here, we report that histidine substitution of Arg-472 located within the A′α-helix of Arabidopsis phot1 to histidine results in constitutively activates kinas activity in vitro without affecting LOV2 photochemistry. Expression analysis of phot1 R472H in the phot-deficient mutant confirmed that it is autophosphorylated in darkness in vivo, but was unable to initiate phot1 signaling in the absence of light. Instead, we found that the phot1 R472H mutant is poorly functional under low-light conditions, but can restore phototropism, chloroplast accumulation, stomatal opening, and leaf positioning and expansion at higher light intensities. Our findings suggest that Arabidopsis can adapt to the elevated phosphorylation status of the phot1 R472H mutant by in part reducing its stability, whereas the activity the mutant under high-light conditions can be attributed to additional increases in LOV2-mediated photoreceptor autophosphorylation

Final Report: The Markets and Marketing Issues of the Kona Coffee Industry

This publication looks some of the problems with markets and marketing of Kona coffee and provides some recommendations for improvement

Delimiting the Culture Defense

This essay builds upon the arguments of Alison Dundes Renteln in her influential book, The Cultural Defense (2004), in which she argues persuasively for a uniformly recognized culture defense in certain litigations. Critiquing some of her details, we recast her three-prong culture defense test to more effectively balance the competing interests of minority culture members to have their ways of life taken seriously by the courts, and of members of the dominant tradition who wish to preserve the rule of law with its necessary perception as treating all parties equally. The offered formulation now includes the following five elements: 1. Is the litigant an enculturated member of the referenced group? 2. Does the group have an acknowledged tradition as that claimed by the litigant? 3. Is that tradition expected to contribute to the fostering of positive social bonds within the culture group? 4. Was the litigant influenced by that tradition when he or she acted? 5. Were the circumstances of the litigant such that he or she could be reasonably presumed to be unaware of the contrary normative standards of the dominant society

Space Station Freedom coupling tasks: An evaluation of their space operational compatibility

The development of the Space Station Freedom tasks that are compatible with both telerobotic as well as extravehicular activity is a necessary redundancy in order to insure successful day to day operation. One task to be routinely performed aboard Freedom will be the changeout of various quick disconnect fluid connectors. In an attempt to resolve these potentially contradictory issues of compatibility, mock-ups of couplings suitable to both extravehicular as well as telerobotic activity were designed and built. An evaluation performed at the Remote Operator Interaction Laboratory at NASA's Johnson Space Center is discussed, which assessed the prototype couplings as well as three standard coupling designs. Data collected during manual and telerobotic manipulation of the couplings indicated that the custom coupling was in fact shown to be faster to operate and generally preferred over the standard coupling designs

Modelling heterogeneity in scale directly: implications for estimates of influence in freight decision-making groups

The state of practice in the modelling of heterogeneous preferences does not separate the effects of scale from estimated mean and standard deviation preference measures. This restriction could lead to divergent behavioural implications relative to a flexible modelling structure that accounts for scale effects independently of estimated distributions of preference measures. The generalised multinomial logit (GMNL) model is such an econometric tool, enabling the analyst to identify the role that scale plays in impacting estimated sample mean and standard deviation preference measures, including confirming whether the appropriate model form approaches standard cases such as mixed logit. The GMNL model is applied in this paper to compare the behavioural implications of the minimum information group inference (MIGI) model within a study of interdependent road freight stakeholders in Sydney, Australia. MIGI estimates within GMNL models are compared with extant mixed logit measures (see Hensher and Puckett, 2008) to confirm whether the implications of the restrictive (with respect to scale) mixed logit model are consistent to those from the more flexible GMNL model. The results confirm the overall implication that transporters appear to hold relative power over supply chain responses to variable road-user charges. However, the GMNL model identifies a broader range of potential group decision-making outcomes and a restricted set of attributes over which heterogeneity in group influence is found than the mixed logit model. Hence, this analysis offers evidence that failing to account for scale heterogeneity may result in inaccurate representations of the bargaining set, and the nature of preference heterogeneity, in general