3,549 research outputs found
Antigenic and genetic evolution of contemporary swine H1 influenza viruses in the United States
Several lineages of influenza A viruses (IAV) currently circulate in North American pigs. Genetic diversity is further increased by transmission of IAV between swine and humans and subsequent evolution. Here, we characterized the genetic and antigenic evolution of contemporary swine H1N1 and H1N2 viruses representing clusters H1-α (1A.1), H1-β (1A.2), H1pdm (1A.3.3.2), H1-γ (1A.3.3.3), H1-δ1 (1B.2.2), and H1-δ2 (1B.2.1) currently circulating in pigs in the United States. The δ1-viruses diversified into two new genetic clades, H1-δ1a (1B.2.2.1) and H1-δ1b (1B.2.2.2), which were also antigenically distinct from the earlier H1-δ1-viruses. Further characterization revealed that a few key amino acid changes were associated with antigenic divergence in these groups. The continued genetic and antigenic evolution of contemporary H1 viruses might lead to loss of vaccine cross-protection that could lead to significant economic impact to the swine industry, and represents a challenge to public health initiatives that attempt to minimize swine-to-human IAV transmission
Microscopic dynamics of thin hard rods
Based on the collision rules for hard needles we derive a hydrodynamic
equation that determines the coupled translational and rotational dynamics of a
tagged thin rod in an ensemble of identical rods. Specifically, based on a
Pseudo-Liouville operator for binary collisions between rods, the Mori-Zwanzig
projection formalism is used to derive a continued fraction representation for
the correlation function of the tagged particle's density, specifying its
position and orientation. Truncation of the continued fraction gives rise to a
generalised Enskog equation, which can be compared to the phenomenological
Perrin equation for anisotropic diffusion. Only for sufficiently large density
do we observe anisotropic diffusion, as indicated by an anisotropic mean square
displacement, growing linearly with time. For lower densities, the Perrin
equation is shown to be an insufficient hydrodynamic description for hard
needles interacting via binary collisions. We compare our results to
simulations and find excellent quantitative agreement for low densities and
qualtitative agreement for higher densities.Comment: 21 pages, 6 figures, v2: clarifications and improved readabilit
Quantum Optics with Surface Plasmons
We describe a technique that enables strong, coherent coupling between
individual optical emitters and guided plasmon excitations in conducting
nano-structures at optical frequencies. We show that under realistic
conditions, optical emission can be almost entirely directed into the plasmon
modes. As an example, we describe an application of this technique involving
efficient generation of single photons on demand, in which the plasmon is
efficiently out-coupled to a dielectric waveguide.Comment: 11 pages, 3 figure
Structure of the met protein and variation of met protein kinase activity among human tumour cell lines.
An in vitro autophosphorylation assay has been used to demonstrate that there is considerable variation in met associated protein kinase among human tumour cell lines. Of particular note was the very high level of autophosphorylation of the 140 kD met protein (p140met) in experiments with A431 human cervical carcinoma cells. In contrast in experiments with Daoy human medulloblastoma cells we failed to detect phosphorylation of p140met; instead a high level of phosphorylation of a 132 kD protein was observed. To help understand the basis for the variation in kinase activity and to learn more about the structure of the mature met protein we have analysed p140met in SDS-polyacrylamide gels under non-reducing conditions. Under these conditions the met protein had an apparent molecular weight of 165,000 indicating that the mature met protein may exist as an alpha beta complex in which p140met (designated the beta subunit) is joined by disulphide bonds to a smaller, 25 kD, alpha-chain. We have identified a potential proteolytic cleavage site with the sequence Lys-Arg-Lys-Lys-Arg-Ser at amino acids 303-308 in the human met protein that may account for cleavage of the met protein into alpha and beta subunits
Force on a neutral atom near conducting microstructures
We derive the non-retarded energy shift of a neutral atom for two different
geometries. For an atom close to a cylindrical wire we find an integral
representation for the energy shift, give asymptotic expressions, and
interpolate numerically. For an atom close to a semi-infinite halfplane we
determine the exact Green's function of the Laplace equation and use it derive
the exact energy shift for an arbitrary position of the atom. These results can
be used to estimate the energy shift of an atom close to etched microstructures
that protrude from substrates.Comment: 7 pages, 5 figure
Mode Bifurcation and Fold Points of Complex Dispersion Curves for the Metamaterial Goubau Line
In this paper the complex dispersion curves of the four lowest-order
transverse magnetic modes of a dielectric Goubau line () are
compared with those of a dispersive metamaterial Goubau line. The vastly
different dispersion curve structure for the metamaterial Goubau line is
characterized by unusual features such as mode bifurcation, complex fold
points, both proper and improper complex modes, and merging of complex and real
modes
Preanalytical, Analytical, and Computational Factors Affect Homeostasis Model Assessment Estimates
OBJECTIVE—We investigated how β-cell function and insulin sensitivity or resistance are affected by the type of blood sample collected or choice of insulin assay and homeostatis model assessment (HOMA) calculator (http://www.dtu.ox.ac.uk)
Pinning and transport of cyclotron/Landau orbits by electromagnetic vortices
Electromagnetic waves with phase defects in the form of vortex lines combined
with a constant magnetic field are shown to pin down cyclotron orbits (Landau
orbits in the quantum mechanical setting) of charged particles at the location
of the vortex. This effect manifests itself in classical theory as a trapping
of trajectories and in quantum theory as a Gaussian shape of the localized wave
functions. Analytic solutions of the Lorentz equation in the classical case and
of the Schr\"odinger or Dirac equations in the quantum case are exhibited that
give precise criteria for the localization of the orbits. There is a range of
parameters where the localization is destroyed by the parametric resonance.
Pinning of orbits allows for their controlled positioning -- they can be
transported by the motion of the vortex lines.Comment: This version differs from the printed paper in having the full titles
of all referenced pape
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