Should Histologic Grade Be Incorporated into the TNM Classification System for Small (T1, T2) Node-Negative Breast Adenocarcinomas?

Prognosis of invasive ductal carcinoma (IDC) strongly correlates with tumor grade as determined by Nottingham combined histologic grade. While reporting grade as low grade/favorable (G1), intermediate grade/moderately favorable (G2), and high grade/unfavorable (G3) is recommended by American Joint Committee on Cancer (AJCC) staging system, existing TNM (Primary Tumor/Regional Lymph Nodes/Distant Metastasis) classification does not directly incorporate these data. For large tumors (T3, T4), significance of histologic grade may be clinically moot as those are nearly always candidates for adjuvant therapy. However, for small (T1, T2) node-negative (N0) tumors, grade may be clinically relevant in influencing treatment decisions, but data on outcomes are sparse and controversial. This retrospective study analyzes clinical outcome in patients with small N0 IDC on the basis of tumor grade. Our results suggest that the grade does not impact clinical outcome in T1N0 tumors. In T2N0 tumors, however, it might be prognostically significant and relevant in influencing decisions regarding the need for additional adjuvant therapy and optimal management

Motion analysis of match-play in elite U12 to U16 age-group soccer players

The aim of this study was to quantify the motion demands of match-play in elite U12 to U16 age-group soccer players. Altogether, 112 players from two professional soccer clubs at five age-group levels (U12–U16) were monitored during competitive matches (n=14) using a 5 Hz non-differential global positioning system (NdGPS). Velocity thresholds were normalized for each age-group using the mean squad times for a flying 10 m sprint test as a reference point. Match performance was reported as total distance, high-intensity distance, very high-intensity distance, and sprint distance. Data were reported both in absolute (m) and relative (m min-1) terms due to a rolling substitute policy. The U15 (1.35±0.09 s) and U16 (1.31±0.06 s) players were significantly quicker than the U12 (1.58±0.10 s), U13 (1.52±0.07 s), and U14 (1.51±0.08 s) players in the flying 10 m sprint test (P U12, U13, U14), high-intensity distance (U16 > U12, U13, U14, U15), very high-intensity distance (U16 4 U12, U13), and sprint distance (U16 > U12, U13) than their younger counterparts (P<0.05). When the data are considered relative to match exposure, few differences are apparent. Training prescription for youth soccer players should consider the specific demands of competitive match-play in each age-group

Promiscuous actions of small molecule inhibitors of the protein kinase D-class IIa HDAC axis in striated muscle

AbstractPKD-mediated phosphorylation of class IIa HDACs frees the MEF2 transcription factor to activate genes that govern muscle differentiation and growth. Studies of the regulation and function of this signaling axis have involved MC1568 and Gö-6976, which are small molecule inhibitors of class IIa HDAC and PKD catalytic activity, respectively. We describe unanticipated effects of these compounds. MC1568 failed to inhibit class IIa HDAC catalytic activity in vitro, and exerted divergent effects on skeletal muscle differentiation compared to a bona fide inhibitor of these HDACs. In cardiomyocytes, Gö-6976 triggered calcium signaling and activated stress-inducible kinases. Based on these findings, caution is warranted when employing MC1568 and Gö-6976 as pharmacological tool compounds to assess functions of class IIa HDACs and PKD

Comparison of Laboratory-Grade and Consumer-Grade Hand-to-Foot Bioelectrical Impedance Analyzers for Body Composition Estimation

Bioelectrical impedance analysis (BIA) is a simple and effective technique to estimate body composition, including body fat percentage (BFP). While these analyzers are a popular method of describing a person’s body composition, laboratory-grade devices are expensive and inaccessible to most people. As a result, they may be an unrealistic method for consumers to use. However, consumer-grade devices are increasingly available. PURPOSE: The purpose of this study was to compare laboratory-grade and consumer-grade bioelectrical impedance analyzers. METHODS: Seventy-five adults (40 F, 35 M) were evaluated using a laboratory-grade, hand-to-foot, multifrequency bioelectrical impedance analyzer (BIALAB; Seca mBCA 515) and a consumer-grade, hand-to-foot, single frequency bioelectrical impedance analyzer (BIACON; Omron HBF-516). Both devices administer undetectable electrical pulses through one extremity that are measured at another extremity, where the voltage drop (impedance) is determined. This information is used to estimate body fluids and composition. RESULTS: A strong, statistically significant correlation between devices was observed for BFP (r: 0.93, R2: 0.87, pCON overestimated BFP by 3.5 ± 3.4% (mean ± SD) relative to BIALAB (BIACON: 28.3 ± 9.6%; BIALAB: 24.8 ± 9.3%; pCONCLUSION: These results collectively suggest that while the laboratory-grade and consumer-grade analyzers in our study exhibit strong correlations when assessing a group of individuals, the consumer-grade device overestimates BFP. Additionally, the SEE indicates that 3.4% error can be expected with the consumer-grade device. Overall, the Omron HBF-516 consumer-grade device may be an adequate and affordable option to estimate body composition in some contexts, but results should be interpreted cautiously when used in individuals

Analyzing the Between-Day Reliability of Three-Dimensional Body Scanners for Body Composition Assessment

In the growing world of health and well-being, three-dimensional (3D) scanning is emerging as a popular tool to assess body composition. While body composition cannot be truly “measured” in living humans, it can be approximated. This, however, leads to two types of errors (i.e., technical and biological) in the body composition assessment. PURPOSE: This study was used to determine what percent or range of percentages in body fat one must exceed before concluding a real change has occurred. By conducting assessments on separate days, with only a short period of time between them, true changes in body composition are unlikely to occur. Therefore, this design can help determine the inherent, between-day error in a body fat assessment to provide context for longer-term changes. METHODS: In the present investigation, thirteen participants were scanned using three distinct 3D body scanners (Fit3D Proscanner, Sizestream SS20, and Styku S100) on two separate mornings, separated by 24 to 48 hours. Each subject had to follow the pre-assessment restrictions and ensure they fit all the eligibility requirements for this study. Then, all body fat percentage (BF%) values from the 3D scanners were recorded and analyzed to determine the between-day reliability. RESULTS: Intraclass correlation coefficients ranged from 0.971 to 0.997 for the three scanners. The least significant change (LSC) values were 1.2%, 2.6%, and 3.0% for the Styku S100, Fit3D Proscanner, and Sizestream SS20, respectively. When examining differences in BF% for individual participants, the between-day differences ranged from -1.1% to 1.0% for Styku S100, -1.9% to 3.2% for Fit3D Proscanner, and -4.0% to 3.0% for SizeStream SS20. CONCLUSION: These results collectively suggest that the Styku S100 has the highest between-day reliability and lowest technical error of the three scanning systems. Overall, however, it is important for consumers to understand that each 3D scanner contains some level of error that should be considered when interpreting the results of an assessment. This study can not only be applied to future research determining the most reliable body composition assessments, but it can also aid individuals in understanding how large of a change in body fat is needed to exceed the error of a 3D scanner and therefore be considered a “real” change

Otto coupling to a transverse-electric-polarized mode on a metamaterial surface

publication-status: Publishedtypes: ArticleAlastair P. Hibbins, Matthew J. Lockyear and J. Roy Sambles, Physical Review B, Vol. 84, article 115130 (2011). Copyright © 2011 by the American Physical Society.Using the Otto geometry, prism coupling of microwave radiation to a metamaterial surface that supports a bound transverse-electric-polarized surface mode is demonstrated. The dispersion of this surface mode is characterised experimentally for incident radiation beyond the critical angle of the prism, and its resonant fields are explored using a numerical model

Recurrent mutation of IGF signalling genes and distinct patterns of genomic rearrangement in osteosarcoma

Osteosarcoma is a primary malignancy of bone that affects children and adults. Here, we present the largest sequencing study of osteosarcoma to date, comprising 112 childhood and adult tumours encompassing all major histological subtypes. A key finding of our study is the identification of mutations in insulin-like growth factor (IGF) signalling genes in 8/112 (7%) of cases. We validate this observation using fluorescence in situ hybridization (FISH) in an additional 87 osteosarcomas, with IGF1 receptor (IGF1R) amplification observed in 14% of tumours. These findings may inform patient selection in future trials of IGF1R inhibitors in osteosarcoma. Analysing patterns of mutation, we identify distinct rearrangement profiles including a process characterized by chromothripsis and amplification. This process operates recurrently at discrete genomic regions and generates driver mutations. It may represent an age-independent mutational mechanism that contributes to the development of osteosarcoma in children and adults alike

The Effects of Mothers' Depression on the Behavioral Assessment of Disruptive Child Behavior

This study uses a group design to compare depressed and non-depressed mothers and their disruptive children. It controls for broad environmental stress factors to examine whether specific differences between groups can be linked with mothers’ depression. It aims to build a more comprehensive picture of depressed mothers’ interactions with their disruptive children by comparing these interactions with those of similar, but non-maternally depressed mother-child dyads, and a non-clinic control group

Prevention of child behavior problems through universal implementation of a group behavioral family intervention.

The aim of this mental health promotion initiative was to evaluate the effectiveness of a universally delivered group behavioral family intervention (BFI) in preventing behavior problems in children. This study investigates the transferability of an efficacious clinical program to a universal prevention intervention delivered through child and community health services targeting parents of preschoolers within a metropolitan health region. A quasiexperimental two-group (BFI, n=804 vs. Comparison group, n=806) longitudinal design followed preschool aged children and their parents over a 2-year period. BFI was associated with significant reductions in parent-reported levels of dysfunctional parenting and parent-reported levels of child behavior problems. Effect sizes on child behavior problems ranged from large (.83) to moderate (.47). Positive and significant effects were also observed in parent mental health, marital adjustment, and levels of child rearing conflict. Findings are discussed with respect to their implication for significant population reductions in child behavior problems as well as the pragmatic challenges for prevention science in encouraging both the evaluation and uptake of preventive initiatives in real world settings