287 research outputs found
Thinking about growth : a cognitive mapping approach to understanding small business development
School of Managemen
Purification and properties of glyceraldehyde-3-phosphate dehydrogenase fromthe skeletalmuscle of the hibernating ground squirrel, ictidomys tridecemlineatus
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from the skeletal muscle of euthermic and torpid Ictidomys tridecemlineatus was purified to electrophoretic homogeneity using a novel method involving Blue-agarose and Phenyl-agarose chromatography. Kinetic analysis of the enzymes isolated from the two conditions suggested the existence of two structurally distinct proteins, with GAPDH Vmax being 40-60% less for the enzyme from the torpid condition (in both glycolytic and gluconeogenic directions) as compared to the euthermic enzyme form. Thermal denaturation, in part determined by differential scanning fluorimetry, revealed that purified GAPDH from the torpid animals was significantly more stable that the enzyme from the euthermic condition. Mass spectrometry combined withWestern blot analyses of purified GAPDH indicate that the cellular GAPDH population is extensively modified, with posttranslational phosphorylation, acetylation and methylation being detected. Global reduction in GAPDH tyrosine phosphorylation during torpor as well as site specific alterations in methylation sites suggests that that the stable changes observed in kinetic and structural GAPDH properties may be due to posttranslational modification of this enzyme during torpor. Taken together, these results suggest a stable suppression of GAPDH (possibly by some reversible posttranslational modification) during ground squirrel torpor, which likely contributes to the overall reduction in carbohydrate metabolism when these animals switch to lipid fuels during dormancy
Fitting the integrated Spectral Energy Distributions of Galaxies
Fitting the spectral energy distributions (SEDs) of galaxies is an almost
universally used technique that has matured significantly in the last decade.
Model predictions and fitting procedures have improved significantly over this
time, attempting to keep up with the vastly increased volume and quality of
available data. We review here the field of SED fitting, describing the
modelling of ultraviolet to infrared galaxy SEDs, the creation of
multiwavelength data sets, and the methods used to fit model SEDs to observed
galaxy data sets. We touch upon the achievements and challenges in the major
ingredients of SED fitting, with a special emphasis on describing the interplay
between the quality of the available data, the quality of the available models,
and the best fitting technique to use in order to obtain a realistic
measurement as well as realistic uncertainties. We conclude that SED fitting
can be used effectively to derive a range of physical properties of galaxies,
such as redshift, stellar masses, star formation rates, dust masses, and
metallicities, with care taken not to over-interpret the available data. Yet
there still exist many issues such as estimating the age of the oldest stars in
a galaxy, finer details ofdust properties and dust-star geometry, and the
influences of poorly understood, luminous stellar types and phases. The
challenge for the coming years will be to improve both the models and the
observational data sets to resolve these uncertainties. The present review will
be made available on an interactive, moderated web page (sedfitting.org), where
the community can access and change the text. The intention is to expand the
text and keep it up to date over the coming years.Comment: 54 pages, 26 figures, Accepted for publication in Astrophysics &
Space Scienc
Cichlid biogeography: comment and review
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72313/1/j.1467-2979.2004.00148.x.pd
Reduction in Subtypes and Sizes of Myocardial Infarction With Ticagrelor in PEGASUS-TIMI 54
Background: Ticagrelor reduced cardiovascular death, myocardial infarction (MI), or stroke in patients with prior MI in PEGASUSTIMI 54 (Prevention of Cardiovascular Events [eg, Death From Heart or Vascular Disease, Heart Attack, or Stroke] in Patients With Prior Heart Attack Using Ticagrelor Compared to Placebo on a Background of Aspirin). MI can occur in diverse settings and with varying severity; therefore, understanding the types and sizes of MI events prevented is of clinical importance.
Methods and Results: MIs were adjudicated by a blinded clinical events committee and categorized by subtype and fold elevation of peak cardiac troponin over the upper limit of normal. A total of 1042 MIs occurred in 898 of the 21 162 randomized patients over a median follow-up of 33 months. The majority of the MIs (76%) were spontaneous (Type 1), with demand MI (Type 2) and stent thrombosis (Type 4b) accounting for 13% and 9%, respectively; sudden death (Type 3), percutaneous coronary interventionârelated (Type 4a) and coronary artery bypass graftârelated (Type 5) each accounted for <1%. Half of MIs (520, 50%) had a peak troponin â„10x upper limit of normal and 21% of MIs (220) had a peak troponin â„1009 upper limit of normal. A total of 21% (224) were ST-segmentâelevation MI STEMI. Overall ticagrelor reduced MI (4.47% versus 5.25%, hazard ratio 0.83, 95% confidence interval 0.72â0.95, P=0.0055). The benefit was consistent among the subtypes, including a 31% reduction in MIs with a peak troponin â„1009 upper limit of normal (hazard ratio 0.69, 95% confidence interval 0.53â0.92, P=0.0096) and a 40% reduction in ST-segment elevation MI (hazard ratio 0.60, 95% confidence interval 0.46â0.78, P=0.0002). Conclusions: In stable outpatients with prior MI, the majority of recurrent MIs are spontaneous and associated with a high biomarker elevation. Ticagrelor reduces the MI consistently among subtypes and sizes including large MIs and ST-segment elevation MI
Supernova Interaction with a Circumstellar Medium
The explosion of a core collapse supernova drives a powerful shock front into
the wind from the progenitor star. A layer of shocked circumstellar gas and
ejecta develops that is subject to hydrodynamic instabilities. The hot gas can
be observed directly by its X-ray emission, some of which is absorbed and
re-radiated at lower frequencies by the ejecta and the circumstellar gas.
Synchrotron radiation from relativistic electrons accelerated at the shock
fronts provides information on the mass loss density if free-free absorption
dominates at early times or the size of the emitting region if synchrotron
self-absorption dominates. Analysis of the interaction leads to information on
the density and structure of the ejecta and the circumstellar medium, and the
abundances in these media. The emphasis here is on the physical processes
related to the interaction.Comment: 22 pages, 7 figures, to appear as a Chapter in "Supernovae and
Gamma-Ray Bursts," edited by K. W. Weiler (Springer-Verlag
A roadmap for Antarctic and Southern Ocean science for the next two decades and beyond
Antarctic and Southern Ocean science is vital to understanding natural variability, the processes
that govern global change and the role of humans in the Earth and climate system. The potential for new
knowledge to be gained from future Antarctic science is substantial. Therefore, the international Antarctic
community came together to âscan the horizonâ to identify the highest priority scientific questions that
researchers should aspire to answer in the next two decades and beyond. Wide consultation was a
fundamental principle for the development of a collective, international view of the most important future
directions in Antarctic science. From the many possibilities, the horizon scan identified 80 key scientific
questions through structured debate, discussion, revision and voting. Questions were clustered into seven
topics: i)Antarctic atmosphere and global connections, ii) Southern Ocean and sea ice in a warming world,
iii) ice sheet and sea level, iv) the dynamic Earth, v) life on the precipice, vi) near-Earth space and beyond,
and vii) human presence in Antarctica. Answering the questions identified by the horizon scan will require
innovative experimental designs, novel applications of technology, invention of next-generation field and
laboratory approaches, and expanded observing systems and networks. Unbiased, non-contaminating
procedures will be required to retrieve the requisite air, biota, sediment, rock, ice and water samples.
Sustained year-round access toAntarctica and the Southern Ocean will be essential to increase winter-time
measurements. Improved models are needed that represent Antarctica and the Southern Ocean in the
Earth System, and provide predictions at spatial and temporal resolutions useful for decision making.
A co-ordinated portfolio of cross-disciplinary science, based on new models of international collaboration,
will be essential as no scientist, programme or nation can realize these aspirations alone.Tinker Foundation, Antarctica New Zealand, The New Zealand
Antarctic Research Institute, the Scientific Committee on
Antarctic Research (SCAR), the Council of Managers of
National Antarctic Programs (COMNAP), the Alfred
Wegner Institut, Helmholtz Zentrum fĂŒr Polar und
Meeresforschung (Germany), and the British Antarctic
Survey (UK).http://journals.cambridge.org/action/displayJournal?jid=ANShb201
The Sudbury Neutrino Observatory
The Sudbury Neutrino Observatory is a second generation water Cherenkov
detector designed to determine whether the currently observed solar neutrino
deficit is a result of neutrino oscillations. The detector is unique in its use
of D2O as a detection medium, permitting it to make a solar model-independent
test of the neutrino oscillation hypothesis by comparison of the charged- and
neutral-current interaction rates. In this paper the physical properties,
construction, and preliminary operation of the Sudbury Neutrino Observatory are
described. Data and predicted operating parameters are provided whenever
possible.Comment: 58 pages, 12 figures, submitted to Nucl. Inst. Meth. Uses elsart and
epsf style files. For additional information about SNO see
http://www.sno.phy.queensu.ca . This version has some new reference
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