Mixed layer temperature response to the southern annular mode: Mechanisms and model representation

Previous studies have shown that simulated sea surface temperature (SST) responses to the southern annular mode (SAM) in phase 3 of the Coupled Model Intercomparison Project (CMIP3) climate models compare poorly to the observed response. The reasons behind these model inaccuracies are explored. The ocean mixed layer heat budget is examined in four of the CMIP3 models and by using observations- reanalyses. The SST response to the SAM is predominantly driven by sensible and latent heat flux and Ekman heat transport anomalies. The radiative heat fluxes play a lesser but nonnegligible role. Errors in the simulated SST responses are traced back to deficiencies in the atmospheric response to the SAM. The models exaggerate the surface wind response to the SAM leading to large unrealistic Ekman transport anomalies. During the positive phase of the SAM, this results in excessive simulated cooling in the 40°-65°S latitudes. Problems with the simulated wind stress responses, which relate partly to errors in the simulated winds themselves and partly to the transfer coefficients used in the models, are a key cause of the errors in the SST response. In the central Pacific sector (90°-150°W), errors arise because the simulated SAM is too zonally symmetric. Substantial errors in the net shortwave radiation are also found, resulting from a poor repre- sentation of the changes in cloud cover associated with the SAM. The problems in the simulated SST re- sponses shown by this study are comparable to deficiencies previously identified in the CMIP3 multimodel mean. Therefore, it is likely that the deficiencies identified here are common to other climate models

Forensic NAPL Determination And Plume Differentiation – A Case Study

Recent developments in laboratory analytical techniques, when combined with application of multivariate statistical analyses allow differentiation and delineation of comingled NAPLs and resultant contaminant plumes. The increase in laboratory expertise and a growing body of relevant studies in case literature have made possible evaluation of petroleum impacted media through relatively standard techniques a reality, and well within the realm of economical site assessments. The application of several methods to determine the number, type, and timing of multiple NAPL releases at a commercial facility in an urban setting will be summarized. The combination of chemical, historical, and statistical techniques has allowed for a more complete evaluation of NAPL sources and release histories than was initially thought possible. In the examined case, overlapping NAPL plumes are defined, delineated, and identified; and source identifications for individual releases are completed. These evaluations resulted in identification of previously unconfirmed sources, improved characterization of the nature of NAPL present, and refined fate, transport, and feasibility evaluations for the conceptual site model

The role of eddies in the Southern Ocean temperature response to the southern annular mode

© Copyright 2009 American Meteorological Society (AMS). Permission to use figures, tables, and brief excerpts from this work in scientific and educational works is hereby granted provided that the source is acknowledged. Any use of material in this work that is determined to be “fair use” under Section 107 of the U.S. Copyright Act September 2010 Page 2 or that satisfies the conditions specified in Section 108 of the U.S. Copyright Act (17 USC §108, as revised by P.L. 94-553) does not require the AMS’s permission. Republication, systematic reproduction, posting in electronic form, such as on a web site or in a searchable database, or other uses of this material, except as exempted by the above statement, requires written permission or a license from the AMS. Additional details are provided in the AMS Copyright Policy, available on the AMS Web site located at (http://www.ametsoc.org/) or from the AMS at 617-227-2425 or [email protected] role of eddies in modulating the Southern Ocean response to the southern annular mode (SAM) is examined, using an ocean model run at multiple resolutions from coarse to eddy resolving. The high-resolution versions of the model show an increase in eddy kinetic energy that peaks 2–3 yr after a positive anomaly in the SAM index. Previous work has shown that the instantaneous temperature response to the SAM is characterized by predominant cooling south of 45°S and warming to the north. At all resolutions the model captures this temperature response. This response is also evident in the coarse-resolution implementation of the model with no eddy mixing parameterization, showing that eddies do not play an important role in the instantaneous response. On the longer time scales, an intensification of the mesoscale eddy field occurs, which causes enhanced poleward heat flux and drives warming south of the oceanic Polar Front. This warming is of greater magnitude and occurs for a longer period than the initial cooling response. The results demonstrate that this warming is surface intensified and strongest in the mixed layer. Non-eddy-resolving models are unable to capture the delayed eddy-driven temperature response to the SAM. The authors therefore question the ability of coarse-resolution models, such as those commonly used in climate simulations, to accurately represent the full impacts of the SAM on the Southern Ocean

Inorganic−Organic Hybrid Luminescent Binary Probe for DNA Detection Based on Spin-Forbidden Resonance Energy Transfer

We describe the design of new fluorescent binary probe sensors for DNA detection based on spin-forbidden resonance energy transfer (SF-RET). Binary probes consist of a donor and acceptor fluorophores that are attached to two different oligonucleotides and serve as a resonance energy transfer (RET) donor−acceptor pair when hybridized to adjacent sites of a target sequence. In the absence of target, excitation of the donor results in fluorescence only from the donor, but when the probes hybridize to the target, the fluorophores are brought into close proximity favoring RET, yielding fluorescence mainly from the acceptor fluorophore. These new binary probes use the metal complex Ru(bpy‘)(DIP)_2^(2+) as the energy donor and an organic fluorophore (Cy5) as the energy acceptor. Energy transfer from the MLCT state of the Ru complex to singlet Cy5 is spin forbidden and produces a delayed fluorescence of Cy5. This paper demonstrates that fluorescence delay of Cy5 can be used to time resolve the emission of the probe from the intense fluorescence background of a model system for cellular background; this provides the reported system to overcome intense autofluorescence, an important and general advantage over “classical” spin-allowed steady-state probes

Combinatorial fluorescence energy transfer molecular beacons for probing nucleic acid sequences

We report the design, synthesis, and characterization of molecular beacons (MB) consisting of three distinct fluorophores, 6-carboxyfluorescein (Fam), N,N,N ,N -tetramethyl-6-carboxyrhodamine (Tam), and Cyanine-5 (Cy5). The primary light absorber/energy donor (Fam) is located on one terminus of the MB, whereas the primary energy acceptor/secondary donor (Tam) and secondary acceptor (Cy5) are located at the other terminus of the MB. In the absence of target DNA or RNA, the MB exists in the stem-closed form. Excitation of Fam initiates an energy transfer cascade from Fam to Tam and further to Cy5 generating unique fluorescence signatures defined as the ratio of the emission from each of the three fluorophores. This energy transfer cascade was investigated in detail by steady-state and time-resolved fluorescence spectroscopy, as well as fluorescence depolarization studies. In the presence of the complementary target DNA, the MB opened efficiently and hybridized with the target separating Fam and Tam by a large distance, so that energy transfer from Fam to Tam was blocked in the stem-open form. This opening of the MB generates a "bar code" fluorescence signature, which is different from the signature of the stem-closed MB. The fluorescence signature of this combinatorial fluorescence energy transfer MB can be tuned by variation of the spacer length between the individual fluorophores

A covalently linked phenanthridine-ruthenium(II) complex as a RNA probe

A phenanthridine derivative covalently linked to a ruthenium complex yields an imaging probe whose fluorescence intensity and lifetime change substantially in the presence of RNA

Human error mitigation initiative (HEMI) : summary report.

Despite continuing efforts to apply existing hazard analysis methods and comply with requirements, human errors persist across the nuclear weapons complex. Due to a number of factors, current retroactive and proactive methods to understand and minimize human error are highly subjective, inconsistent in numerous dimensions, and are cumbersome to characterize as thorough. An alternative and proposed method begins with leveraging historical data to understand what the systemic issues are and where resources need to be brought to bear proactively to minimize the risk of future occurrences. An illustrative analysis was performed using existing incident databases specific to Pantex weapons operations indicating systemic issues associated with operating procedures that undergo notably less development rigor relative to other task elements such as tooling and process flow. Future recommended steps to improve the objectivity, consistency, and thoroughness of hazard analysis and mitigation were delineated

Synthesis of 3-D coronal-solar wind energetic particle acceleration modules

1. Introduction Acute space radiation hazards pose one of the most serious risks to future human and robotic exploration. Large solar energetic particle (SEP) events are dangerous to astronauts and equipment. The ability to predict when and where large SEPs will occur is necessary in order to mitigate their hazards. The Coronal-Solar Wind Energetic Particle Acceleration (C-SWEPA) modeling effort in the NASA/NSF Space Weather Modeling Collaborative [Schunk, 2014] combines two successful Living With a Star (LWS) (http://lws. gsfc.nasa.gov/) strategic capabilities: the Earth-Moon-Mars Radiation Environment Modules (EMMREM) [Schwadron et al., 2010] that describe energetic particles and their effects, with the Next Generation Model for the Corona and Solar Wind developed by the Predictive Science, Inc. (PSI) group. The goal of the C-SWEPA effort is to develop a coupled model that describes the conditions of the corona, solar wind, coronal mass ejections (CMEs) and associated shocks, particle acceleration, and propagation via physics-based modules. Assessing the threat of SEPs is a difficult problem. The largest SEPs typically arise in conjunction with X class flares and very fast (\u3e1000 km/s) CMEs. These events are usually associated with complex sunspot groups (also known as active regions) that harbor strong, stressed magnetic fields. Highly energetic protons generated in these events travel near the speed of light and can arrive at Earth minutes after the eruptive event. The generation of these particles is, in turn, believed to be primarily associated with the shock wave formed very low in the corona by the passage of the CME (injection of particles from the flare site may also play a role). Whether these particles actually reach Earth (or any other point) depends on their transport in the interplanetary magnetic field and their magnetic connection to the shock

Trends in kidney function testing in UK primary care since the introduction of the Quality and Outcomes Framework:a retrospective cohort study using CPRD

Objectives: To characterise serum creatinine and urinary protein testing in UK general practices from 2005 to 2013 and to examine how the frequency of testing varies across demographic factors, with the presence of chronic conditions and with the prescribing of drugs for which kidney function monitoring is recommended. Design: Retrospective open cohort study. Setting: Routinely collected data from 630 UK general practices contributing to the Clinical Practice Research Datalink. Participants: 4 573 275 patients aged over 18 years registered at up-to-standard practices between 1 April 2005 and 31 March 2013. At study entry, no patients were kidney transplant donors or recipients, pregnant or on dialysis. Primary outcome measures: The rate of serum creatinine and urinary protein testing per year and the percentage of patients with isolated and repeated testing per year. Results: The rate of serum creatinine testing increased linearly across all age groups. The rate of proteinuria testing increased sharply in the 2009–2010 financial year but only for patients aged 60 years or over. For patients with established chronic kidney disease (CKD), creatinine testing increased rapidly in 2006–2007 and 2007–2008, and proteinuria testing in 2009–2010, reflecting the introduction of Quality and Outcomes Framework indicators. In adjusted analyses, CKD Read codes were associated with up to a twofold increase in the rate of serum creatinine testing, while other chronic conditions and potentially nephrotoxic drugs were associated with up to a sixfold increase. Regional variation in serum creatinine testing reflected country boundaries. Conclusions: Over a nine-year period, there have been increases in the numbers of patients having kidney function tests annually and in the frequency of testing. Changes in the recommended management of CKD in primary care were the primary determinant, and increases persist even after controlling for demographic and patient-level factors. Future studies should address whether increased testing has led to better outcomes.</p