669 research outputs found

    Identification of putative methanol dehydrogenase (moxF) structural genes in methylotrophs and cloning of moxF genes from Methylococcus capsulatus bath and Methylomonas albus BG8

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    An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putative moxF genes from two obligate methanotrophic bacteria, Methylococcus capsulatus Bath and Methylomonas albus BG8. The identity of these genes was confirmed in two ways. A T7 expression vector was used to produce methanol dehydrogenase protein in Escherichia coli from the cloned genes, and in each case the protein was identified by immunoblotting with antiserum against the Methylomonas albus methanol dehydrogenase. In addition, a moxF mutant of Methylobacterium strain AM1 was complemented to a methanol-positive phenotype that partially restored methanol dehydrogenase activity, using broad-host-range plasmids containing the moxF genes from each methanotroph. The partial complementation of a moxF mutant in a facultative serine pathway methanol utilizer by moxF genes from type I and type X obligate methane utilizers suggests broad functional conservation of the methanol oxidation system among gram-negative methylotrophs

    Nothing More Delicious: Food as Temptation in Children\u27s Literature

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    Although many critics and theorists, including Roland Barthes, have discussed food in literature, little attention has been paid to the food-as-temptation story in childrenā€™s literature. In Lewis Carrollā€™s Aliceā€™s Adventures in Wonderland, C.S. Lewisā€™ The Lion, the Witch, and the Wardrobe, Roald Dahlā€™s Charlie and the Chocolate Factory, and Neil Gaimanā€™s Coraline food is used as temptation for child protagonists, a tool to lure them into doing evil deeds or being generally mischievous. Some characters, like Alice, act as the tempters as well as the tempted, while others, like Edmund, wait passively for rescue. Coraline breaks this model by featuring a protagonist that not only saves her kidnapped parents by using food as a weapon, even after she is tempted by food herself. Coraline represents a more contemporary version of the food-as-temptation because its protagonistā€™s agency is more complex. Coraline dictates her relationship with food, which makes her differ from other protagonists in other fantastical childrenā€™s works

    Do inhaled beta-agonists control cough in URIs or acute bronchitis?

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    Patients who receive inhaled beta-agonists for cough due to acute upper respiratory infections (URI) are just as likely to report a productive cough at 7 days compared with patients treated with placebo (strength of recommendation [SOR]: A, based on a systematic review). One trial, however, showed a reduction in overall cough at 7 days (number needed to treat [NNT]=3, SOR: B, a small randomized controlled trial), and another trial found a reduction in overall symptom score in smokers and those with wheezing on initial exam (SOR: B, based on a small randomized controlled trial)

    Black Women as Learners: Adult Education and Family Literacy Program Participation among Primary Caregiving Grandmothers

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    The purpose of this study was to examine how Black grandmothers who are the primary caregivers promote literacy for themselves and for the grandchild or grandchildren in their care. The findings were situated into a three category model (author developed). Implications for research and practice are also provided

    Social Capital and Caregiving among Grandmothers in Rural Communities

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    The purpose of this study was to explore the experiences of ten Black primary caregiving grandmothers in rural communities within the state of Texas to understand how they learn to develop social capital to navigate inequitable systems and acquire resources for themselves and the grandchildren in their care. The application of qualitative inquiry was essential as it best supported the probing necessary to explore the phenomenon of African American grandmothers raising grandchildren

    Letters between Mary Pettit and William Kerr\u27s secretary

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    Letters concerning a position in Domestic Science at Utah Agricultural College

    Glucosamine hydrochloride for the treatment of osteoarthritis symptoms

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    Osteoarthritis is the most common arthritis in the world. It affects millions of people with age being the greatest risk factor for developing the disease. The burden of disease will worsen with the aging of the worldā€™s population. The disease causes pain and functional disability. The direct costs of osteoarthritis include hospital and physician visits, medications, and assistive services. The indirect costs include work absences and lost wages. Many studies have sought to find a therapy to relieve pain and reduce disability. Glucosamine hydrochloride (HCl) is one of these therapies. There are limited studies of glucosamine HCl in humans. Although some subjects do report statistically significant improvement in pain and function from products combining glucosamine HCl and other agents, glucosamine HCl by itself appears to offer little benefit to those suffering from osteoarthritis

    Which tool is most useful in diagnosing bipolar disorder in children?

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    No single, well-validated screening instrument for clinical diagnosis of bipolar disorder in children exists. That said, the Kiddie Schedule for Affective Disorders and Schizophrenia (KSADS), a semi-structured interview, along with clinical evaluation by a childhood mental health specialist, is used most frequently in major research studies (strength of recommendation [SOR]: C)
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