Strategic Group Analysis of U.S. Food Businesses Using the Two-step Clustering Method

strategic group, planning, strategy, performance, Agribusiness, Institutional and Behavioral Economics, Marketing, Productivity Analysis, M0, M14, M2, M30,

Effects of Lift Velocity on Muscle Activation During Leg Extension

It is not known if manipulating velocity within a prescribed resistance training mode will improve muscle activation. Muscle activations of the Rectus Femoris (RF), Vastus Lateralis (VL), Vastus Medialis (VM) and Bicep Femoris (BF) were examined during a leg extension exercise at 3 different velocities on 15 subjects (10men, 5 female, Age = 21.5 ± 1.8 yrs, Height = 171.2 ± 12.5 cm, Mass = 75.5 ± 16.3 kg). Trials of 1 set of 10 repetitions at 60% of 1RM, were performed at 15, 30 and 60º/s. Bipolar surface electrodes were placed over the BF, RF, VL, and VM. Micro-switches were utilized to identify the concentric (CON) and eccentric (ECC) phases of the lift. Data were sampled at 1024 Hz, filtered, rectified and the mean, integrated EMG calculated. One 2 x 4 x 3 (action x muscle x velocity) ANOVA with bonferonni adjustment was run and significance was followed by Tukey HSD post hoc analysis. Results indicated significantly greater activation of the VL, RF and VM for ECC extension at 60º/s compared to 15º/s. While 60º/s was also greater than 30º/s for the VL and VM during ECC. While comparing muscle action, CON VL, VM and RF were greater than ECC at 30º/sec, meanwhile VM CON was also greater at 15º/sec. No differences in muscle activation at any velocity or muscle action for BF were identified. We conclude that muscle recruitment while training with a 60% 1RM load is maximized at a velocity of 60º/s during ECC activity and 15 or 30º/sec during CON

DNA Sequence Determinants Controlling Affinity, Stability and Shape of DNA Complexes Bound by the Nucleoid Protein Fis.

The abundant Fis nucleoid protein selectively binds poorly related DNA sequences with high affinities to regulate diverse DNA reactions. Fis binds DNA primarily through DNA backbone contacts and selects target sites by reading conformational properties of DNA sequences, most prominently intrinsic minor groove widths. High-affinity binding requires Fis-stabilized DNA conformational changes that vary depending on DNA sequence. In order to better understand the molecular basis for high affinity site recognition, we analyzed the effects of DNA sequence within and flanking the core Fis binding site on binding affinity and DNA structure. X-ray crystal structures of Fis-DNA complexes containing variable sequences in the noncontacted center of the binding site or variations within the major groove interfaces show that the DNA can adapt to the Fis dimer surface asymmetrically. We show that the presence and position of pyrimidine-purine base steps within the major groove interfaces affect both local DNA bending and minor groove compression to modulate affinities and lifetimes of Fis-DNA complexes. Sequences flanking the core binding site also modulate complex affinities, lifetimes, and the degree of local and global Fis-induced DNA bending. In particular, a G immediately upstream of the 15 bp core sequence inhibits binding and bending, and A-tracts within the flanking base pairs increase both complex lifetimes and global DNA curvatures. Taken together, our observations support a revised DNA motif specifying high-affinity Fis binding and highlight the range of conformations that Fis-bound DNA can adopt. The affinities and DNA conformations of individual Fis-DNA complexes are likely to be tailored to their context-specific biological functions

Forecasting constraints from the cosmic microwave background on eternal inflation

We forecast the ability of cosmic microwave background (CMB) temperature and polarization datasets to constrain theories of eternal inflation using cosmic bubble collisions. Using the Fisher matrix formalism, we determine both the overall detectability of bubble collisions and the constraints achievable on the fundamental parameters describing the underlying theory. The CMB signatures considered are based on state-of-the-art numerical relativistic simulations of the bubble collision spacetime, evolved using the full temperature and polarization transfer functions. Comparing a theoretical cosmic-variance-limited experiment to the WMAP and Planck satellites, we find that there is no improvement to be gained from future temperature data, that adding polarization improves detectability by approximately 30%, and that cosmic-variance-limited polarization data offer only marginal improvements over Planck. The fundamental parameter constraints achievable depend on the precise values of the tensor-to-scalar ratio and energy density in (negative) spatial curvature. For a tensor-to-scalar ratio of $0.1$ and spatial curvature at the level of $10^{-4}$, using cosmic-variance-limited data it is possible to measure the width of the potential barrier separating the inflating false vacuum from the true vacuum down to $M_{\rm Pl}/500$, and the initial proper distance between colliding bubbles to a factor $\pi/2$ of the false vacuum horizon size (at three sigma). We conclude that very near-future data will have the final word on bubble collisions in the CMB.Comment: 14 pages, 6 figure

First Observational Tests of Eternal Inflation

The eternal inflation scenario predicts that our observable Universe resides inside a single bubble embedded in a vast inflating multiverse. We present the first observational tests of eternal inflation, performing a search for cosmological signatures of collisions with other bubble universes in cosmic microwave background data from the WMAP satellite. We conclude that the WMAP 7-year data do not warrant augmenting the cold dark matter model with a cosmological constant with bubble collisions, constraining the average number of detectable bubble collisions on the full sky N̅ _s<1.6 at 68% C.L. Data from the Planck satellite can be used to more definitively test the bubble-collision hypothesis

Blinded patient preference for morphine compared to placebo in the setting of chronic refractory breathlessness – an exploratory study

Context Patients’ preference for morphine therapy has received little attention in the setting of chronic refractory breathlessness. However, this is one important factor in considering longer term therapy. Objectives The aim of this secondary analysis is to explore blinded patient preference of morphine compared to placebo for this indication and to define any predictors of preference. Methods Data were pooled from three randomized, double-blind, crossover, placebo-controlled studies of morphine (four days each) in chronic refractory breathlessness. Blinded patient preferences were chosen at the end of each study. A multivariable regression model was used to establish patient predictors of preference. Results Sixty-five participants provided sufficient data (60 males; median age 74 years; heart failure 55%, chronic obstructive pulmonary disease 45%; median Eastern Cooperative Oncology Group performance status 2). Forty-three percent of participants preferred morphine (32% placebo and 25% no preference). Morphine preference and younger age were strongly associated: odds ratio = 0.85, 95% confidence interval 0.78, 0.93;

Clonal analysis of kit ligand a functional expression reveals lineage-specific competence to promote melanocyte rescue in the mutant regenerating caudal fin

The study of regeneration in an in vivo vertebrate system has the potential to reveal targetable genes and pathways that could improve our ability to heal and repair damaged tissue. We have developed a system for clonal labeling of discrete cell lineages and independently inducing gene expression under control of the heat shock promoter in the zebrafish caudal fin. Consequently we are able to test the affects of overexpressing a single gene in the context of regeneration within each of the nine different cell lineage classes that comprise the caudal fin. This can test which lineage is necessary or sufficient to provide gene function. As a first example to demonstrate this approach, we explored which lineages were competent to functionally express the kit ligand a protein as assessed by the local complementation of the mutation in the sparse-like (kitlgatc244b) background. We show that dermal fibroblast expression of kit ligand a robustly supports the rescue of melanocytes in the regenerating caudal fin. kit ligand a expression from skin and osteoblasts results in more modest and variable rescue of melanocytes, while lateral line expression was unable to complement the mutation

A robust constraint on cosmic textures from the cosmic microwave background

Fluctuations in the cosmic microwave background (CMB) contain information which has been pivotal in establishing the current cosmological model. These data can also be used to test well-motivated additions to this model, such as cosmic textures. Textures are a type of topological defect that can be produced during a cosmological phase transition in the early universe, and which leave characteristic hot and cold spots in the CMB. We apply Bayesian methods to carry out a rigorous test of the texture hypothesis, using full-sky data from the Wilkinson Microwave Anisotropy Probe. We conclude that current data do not warrant augmenting the standard cosmological model with textures. We rule out at 95% confidence models that predict more than 6 detectable cosmic textures on the full sky.Comment: 5 pages, 2 figures. v2: replaced with version accepted by PRL (minor amendments to reduce length and address referee comments

Malpractice Liability Related to Foreign Outsourcing of Legal Services

The outsourcing of client-related tasks to service providers in other countries is likely to generate malpractice claims against American law firms. This Article discusses the wide range of theories under which an outsourcing American law firm may be liable for its own negligence or for the actions of outsourcing providers. These theories include negligence by the outsourcing law firm, vicarious liability for the conduct of firm principals and employees, vicarious liability for the conduct of independent contractors, and vicarious liability for the conduct of business partners