292 research outputs found

    Manual for the Fish Population Surveys (DOC9 Package) for the District Fisheries Analysis System (FAS)

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    Update of Aquatic Biology Technical Report 87/11; final report of project F-69-R (1-3), Data Base Management and Analysis of Fisheries in ImpoundmentsReport issued on: issued October 1990INHS Technical Report prepared for Illinois Department of Conservatio

    The Fisheries Analysis System (FAS): Creel Survey and Lake Analyses

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    F-69-R(1-3)Report issued on: October 1990Final report of Project F-69-R(1-3), Data Base Management and Analysis of Fisheries in Impoundments, conducted under of memorandum of understanding between Illinois Department of Conservation and the University of Illinois, supported through Fed(TRUNCATED

    What is a Robot?

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    A robot is a mechanical hand and arm, controlled by a computer. It is nothing more than another type of machine. Its ancestry combines two different, but related, technologies: mechanisation and control. The history of the computer has been essential to both. The history of mechanisation began with Oliver Evans' automated mill (1784), continued with Joseph Jacquard's loom (1801), and reached a high state of perfection at the end of the nineteenth century with Steward Babbitt's designs for a motorised crane which had a mechanical gripper to remove ingots from furnaces (1892). In the 1820s the technology of mechanisation cross-fertilised with the emerging science of information and control technology when the English mathematician, Charles Babbage, sometimes known as 'the father of the computer', developed an automatic calculator which he called his 'Difference Engine' (1823). Joseph Jacquard's loom proved to be the plateau from which all subsequent innovations in mechanisation and control took off. His invention was software, the novel idea that you could program a weaver's loom with punched cards that carried a coded 'model' of the patterns being woven. The Jacquard loom appeared in 1801, the last and most significant of a series of innovations in silk weaving which came out of Lyons from the early nineteenth century. It was so successful that by 1812 there were more than 11,000 in France alone. The punched card was a breakthrough in information technology: a Jacquard loom could carry as much as three megabytes of information on perforated paper. This technique of information storage became one of the fundamental components of the automatic memory calculators which gave birth to computers

    Stochastic Sensing of Nanomolar Inositol 1,4,5-Trisphosphate with an Engineered Pore

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    AbstractThe introduction of a ring of arginine residues near the constriction in the transmembrane β barrel of the staphylococcal α-hemolysin heptamer yielded a pore that could be almost completely blocked by phosphate anions at pH 7.5. Block did not occur with other oxyanions, including nitrate, sulfate, perchlorate, and citrate. Based on this finding, additional pores were engineered with high affinities for important cell signaling molecules, such as the Ca2+-mobilizing second messenger inositol 1,4,5-trisphosphate (IP3), that contain phosphate groups. One of these engineered pores, PRR-2, provides a ring of fourteen arginines that project into the lumen of the transmembrane barrel. Remarkably, PRR-2 bound IP3 with low nanomolar affinity while failing to bind another second messenger, adenosine 3′, 5′-cyclic monophosphate (cAMP). The engineered α-hemolysin pores may be useful as components of stochastic sensors for cell signaling molecules

    High temperature ion channels and pores

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    The present invention includes an apparatus, system and method for stochastic sensing of an analyte to a protein pore. The protein pore may be an engineer protein pore, such as an ion channel at temperatures above 55.degree. C. and even as high as near 100.degree. C. The analyte may be any reactive analyte, including chemical weapons, environmental toxins and pharmaceuticals. The analyte covalently bonds to the sensor element to produce a detectable electrical current signal. Possible signals include change in electrical current. Detection of the signal allows identification of the analyte and determination of its concentration in a sample solution. Multiple analytes present in the same solution may also be detected

    Metabolomics in the Analysis of Inflammatory Diseases

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    Most infections and traumatic injuries are cleared or repaired relatively rapidly and metabolic homoeostasis is soon restored. However, there is a broad range of inflammatory diseases which involve chronic activation of the immune system and, as a result, chronic persistent inflammation. We have been studying the metabolic consequences of chronic inflammatory diseases with the aim of identifying metabolic fingerprints which may provide clues about why the localised tissue disease persists

    Prolonged Residence Time of a Noncovalent Molecular Adapter, β-Cyclodextrin, within the Lumen of Mutant ι-Hemolysin Pores

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    Noncovalent molecular adapters, such as cyclodextrins, act as binding sites for channel blockers when lodged in the lumen of the ι-hemolysin (ιHL) pore, thereby offering a basis for the detection of a variety of organic molecules with ιHL as a sensor element. β-Cyclodextrin (βCD) resides in the wild-type ιHL pore for several hundred microseconds. The residence time can be extended to several milliseconds by the manipulation of pH and transmembrane potential. Here, we describe mutant homoheptameric ιHL pores that are capable of accommodating βCD for tens of seconds. The mutants were obtained by site-directed mutagenesis at position 113, which is a residue that lies near a constriction in the lumen of the transmembrane β barrel, and fall into two classes. Members of the tight-binding class, M113D, M113N, M113V, M113H, M113F and M113Y, bind βCD ∟10(4)-fold more avidly than the remaining ιHL pores, including WT-ιHL. The lower K (d) values of these mutants are dominated by reduced values of k(off). The major effect of the mutations is most likely a remodeling of the binding site for βCD in the vicinity of position 113. In addition, there is a smaller voltage-sensitive component of the binding, which is also affected by the residue at 113 and may result from transport of the neutral βCD molecule by electroosmotic flow. The mutant pores for which the dwell time of βCD is prolonged can serve as improved components for stochastic sensors
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