Impacts of horizontal resolution on simulated climate statistics in ECHAM4

The sensitivity of a general circulation model to changes in resolution is studied using the Max Planck Institute for Meteorology (MPI) 19-level model, ECHAM4. Simulations extending over a period between 10 and 15 years, with observed sea surface temperatures as lower boundary conditions from 1979 onward, have been performed using four different horizontal resolutions, T21, T30, T42 and T106. The atmospheric time-mean state and the intraseasonal variability are compared to the European Centre for Medium Range Weather Forecasts (ECMWF) reanalyses and a few other observational datasets. The T30, T42 and T106 simulations are similar in many respects, whereas the T21 simula- tion is qualitatively different. Several effects related to model resolution could be identi- fied, such as increasing tropical upper tropospheric warming with increasing resolution. This is due to more vigorous tropical convection, larger ice water content and, hence, increasing cirrus cloud greenhouse effect. Associated with this increasing warming at higher resolution is a poleward expansion of the zonally averaged circulation regime. On the other hand, the zonally asymmetric component of the circulation, i.e., the stationary waves, improve with higher resolution. Also, higher resolution has a positive impact on regional precipitation patterns which are affected by orography such as the summer mon- soon precipitation over India. Intraseasonal variability has been analyzed only for the higher resolution models, T42 and T106. Compared to the ECMWF reanalyses, both models are able to simulate the intrasea- sonal geopotential height variability, eddy fluxes of heat and momentum, and eddy kinetic energy with reasonable accuracy. This applies to transient eddies in both the bandpass and lowpass regime and to the stationary eddies as well. Some biases can be identified which are more or less independent of resolution. These include the mislocation of the Azores high and the overestimation of its intensity, a cold bias in the polar upper troposphere and lower stratosphere and the poleward and upward displacement of the maxima of geopotential height variability, momentum fluxes and eddy kinetic energy. An important finding is that the operational ECMWF analyses, which have been widely used for model validation, considerably overestimate the lowpass variability, as compared to the reanalyses, due to frequent changes of the forecast model and data assimilation scheme. This implies that the results from our investigations are not directly comparable to previous investigations that used operational analyses for validation

Future permafrost conditions along environmental gradients in Zackenberg, Greenland

The future development of ground temperatures in permafrost areas is determined by a number of factors varying on different spatial and temporal scales. For sound projections of impacts of permafrost thaw, scaling procedures are of paramount importance. We present numerical simulations of present and future ground temperatures at 10 m resolution for a 4 km long transect across the lower Zackenberg valley in northeast Greenland. The results are based on stepwise downscaling of future projections derived from general circulation model using observational data, snow redistribution modeling, remote sensing data and a ground thermal model. A comparison to in situ measurements of thaw depths at two CALM sites and near-surface ground temperatures at 17 sites suggests agreement within 0.10 m for the maximum thaw depth and 1 °C for annual average ground temperature. Until 2100, modeled ground temperatures at 10 m depth warm by about 5 °C and the active layer thickness increases by about 30%, in conjunction with a warming of average near-surface summer soil temperatures by 2 °C. While ground temperatures at 10 m depth remain below 0 °C until 2100 in all model grid cells, positive annual average temperatures are modeled at 1 m depth for a few years and grid cells at the end of this century. The ensemble of all 10 m model grid cells highlights the significant spatial variability of the ground thermal regime which is not accessible in traditional coarse-scale modeling approaches

Permafrost degradation risk zone assessment using simulation models

In this proof-of-concept study we focus on linking large scale climate and permafrost simulations to small scale engineering projects by bridging the gap between climate and permafrost sciences on the one hand and on the other technical recommendation for adaptation of planned infrastructures to climate change in a region generally underlain by permafrost. We present the current and future state of permafrost in Greenland as modelled numerically with the GIPL model driven by HIRHAM climate projections up to 2080. We develop a concept called Permafrost Thaw Potential (PTP), defined as the potential active layer increase due to climate warming and surface alterations. PTP is then used in a simple risk assessment procedure useful for engineering applications. The modelling shows that climate warming will result in continuing wide-spread permafrost warming and degradation in Greenland, in agreement with present observations. We provide examples of application of the risk zone assessment approach for the two towns of Sisimiut and Ilulissat, both classified with high PTP

Gene expression analysis of cell death induction by Taurolidine in different malignant cell lines

<p>Abstract</p> <p>Background</p> <p>The anti-infective agent Taurolidine (TRD) has been shown to have cell death inducing properties, but the mechanism of its action is largely unknown. The aim of this study was to identify potential common target genes modulated at the transcriptional level following TRD treatment in tumour cell lines originating from different cancer types.</p> <p>Methods</p> <p>Five different malignant cell lines (HT29, Chang Liver, HT1080, AsPC-1 and BxPC-3) were incubated with TRD (100 μM, 250 μM and 1000 μM). Proliferation after 8 h and cell viability after 24 h were analyzed by BrdU assay and FACS analysis, respectively. Gene expression analyses were carried out using the <it>Agilent </it>-microarray platform to indentify genes which displayed conjoint regulation following the addition of TRD in all cell lines. Candidate genes were subjected to <it>Ingenuity Pathways Analysis </it>and selected genes were validated by qRT-PCR and Western Blot.</p> <p>Results</p> <p>TRD 250 μM caused a significant inhibition of proliferation as well as apoptotic cell death in all cell lines. Among cell death associated genes with the strongest regulation in gene expression, we identified pro-apoptotic transcription factors (EGR1, ATF3) as well as genes involved in the ER stress response (PPP1R15A), in ubiquitination (TRAF6) and mitochondrial apoptotic pathways (PMAIP1).</p> <p>Conclusions</p> <p>This is the first conjoint analysis of potential target genes of TRD which was performed simultaneously in different malignant cell lines. The results indicate that TRD might be involved in different signal transduction pathways leading to apoptosis.</p

Observation of Blue Corona Discharges and Cloud Microphysics in the Top of Thunderstorm Cells in Cyclone Fani

Blue corona discharges are often observed at the top of thunderclouds. They are bursts of streamers, but the cloud conditions that enable them are not well known. Here we present observations by the Atmosphere-Space Interactions Monitor (ASIM) of 92 discharges during its ∼1 min pass over tropical cyclone Fani in the Bay of Bengal from 20:10:55 to 20:12:05 UTC on 30 April 2019. The discharges were observed in convective cells forming in the rainbands of the cyclone where Convective Available Potential Energy reached ∼6,000 J kg. The Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observation satellite passed over one of the cells ∼12 min after ASIM from 20:23:58 to 20:24:14 UTC. It measured the cloud microphysics related to the discharges and indicated they occurred in a convection region with the cloud top overshooting for over 20 min. The updraft lifted ice particles to lower stratospheric altitudes and formed the gullwing-shaped cirrus. The discharges are found at an average altitude of ∼16 km where the cloud environment contained ∼2 × 10 m ice particles with ∼50 µm radius, resulting in a photon mean free path of ∼3 m. Around 20% of the blue corona discharges coincide with Narrow Bipolar Events indentified from the Earth Networks Total Lightning Network. Our observations suggest that the overshooting cloud top formed by deep convection and a surge in lightning activity facilitated conditions for the blue corona discharges. This work provides the first-ever estimate of important microphysical parameters related to blue corona discharges based on data measurements, establishing a reference for future empirical and theoretical studies. © 2023. The Authors.This work was supported by Independent Research Fund Denmark (Danmarks Frie Forskningsfond) under Grant agreement 1026-00420B. The project has received funding from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant agreement SAINT 722337. ASIM is a mission of the European Space Agency (ESA) and is funded by ESA and by national grants of Denmark, Norway and Spain. The ASIM Science Data Center is supported by ESA PRODEX contracts C 4000115884 (DTU) and 4000123438 (Bergen). With funding from the Spanish government through the "Severo Ochoa Centre of Excellence" accreditation (CEX2021-001131-S)

Potential climatic transitions with profound impact on Europe

We discuss potential transitions of six climatic subsystems with large-scale impact on Europe, sometimes denoted as tipping elements. These are the ice sheets on Greenland and West Antarctica, the Atlantic thermohaline circulation, Arctic sea ice, Alpine glaciers and northern hemisphere stratospheric ozone. Each system is represented by co-authors actively publishing in the corresponding field. For each subsystem we summarize the mechanism of a potential transition in a warmer climate along with its impact on Europe and assess the likelihood for such a transition based on published scientific literature. As a summary, the ‘tipping’ potential for each system is provided as a function of global mean temperature increase which required some subjective interpretation of scientific facts by the authors and should be considered as a snapshot of our current understanding. <br/

Study on the clinical application of pulsed DC magnetic technology for tracking of intraoperative head motion during frameless stereotaxy

BACKGROUND: Tracking of post-registration head motion is one of the major problems in frameless stereotaxy. Various attempts in detecting and compensating for this phenomenon rely on a fixed reference device rigidly attached to the patient's head. However, most of such reference tools are either based on an invasive fixation technique or have physical limitations which allow mobility of the head only in a restricted range of motion after completion of the registration procedure. METHODS: A new sensor-based reference tool, the so-called Dynamic Reference Frame (DRF) which is designed to allow an unrestricted, 360° range of motion for the intraoperative use in pulsed DC magnetic navigation was tested in 40 patients. Different methods of non-invasive attachment dependent on the clinical need and type of procedure, as well as the resulting accuracies in the clinical application have been analyzed. RESULTS: Apart from conventional, completely rigid immobilization of the head (type A), four additional modes of head fixation and attachment of the DRF were distinguished on clinical grounds: type B1 = pin fixation plus oral DRF attachment; type B2 = pin fixation plus retroauricular DRF attachment; type C1 = free head positioning with oral DRF; and type C2 = free head positioning with retroauricular DRF. Mean fiducial registration errors (FRE) were as follows: type A interventions = 1.51 mm, B1 = 1.56 mm, B2 = 1.54 mm, C1 = 1.73 mm, and C2 = 1.75 mm. The mean position errors determined at the end of the intervention as a measure of application accuracy were: 1.45 mm in type A interventions, 1.26 mm in type B1, 1.44 mm in type B2, 1.86 mm in type C1, and 1.68 mm in type C2. CONCLUSION: Rigid head immobilization guarantees most reliable accuracy in various types of frameless stereotaxy. The use of an additional DRF, however, increases the application scope of frameless stereotaxy to include e.g. procedures in which rigid pin fixation of the cranium is not required or desired. Thus, continuous tracking of head motion allows highly flexible variation of the surgical strategy including intraoperative repositioning of the patient without impairment of navigational accuracy as it ensures automatic correction of spatial distortion. With a dental cast for oral attachment and the alternative option of non-invasive retroauricular attachment, flexibility in the clinical use of the DRF is ensured

International Paediatric Mitochondrial Disease Scale

Objective: There is an urgent need for reliable and universally applicable outcome measures for children with mitochondrial diseases. In this study, we aimed to adapt the currently available Newcastle Paediatric Mitochondrial Disease Scale (NPMDS) to the International Paediatric Mitochondrial Disease Scale (IPMDS) during a Delphi-based process with input from international collaborators, patients and caretakers, as well as a pilot reliability study in eight patients. Subsequently, we aimed to test the feasibility, construct validity and reliability of the IPMDS in a multicentre study. Methods: A clinically, biochemically and genetically heterogeneous group of 17 patients (age 1.6–16 years) from five different expert centres from four different continents were evaluated in this study. Results: The feasibility of the IPMDS was good, as indicated by a low number of missing items (4 %) and the positive evaluation of patients, parents and users. Principal component analysis of our small sample identified three factors, which explained 57.9 % of the variance. Good construct validity was found using hypothesis testing. The overall interrater reliability was good [median intraclass correlation coefficient for agreement between raters (ICCagreement) 0.85; range 0.23–0.99). Conclusion: In conclusion, we suggest using the IPMDS for assessing natural history in children with mitochondrial diseases. These data should be used to further explore construct validity of the IPMDS and to set age limits. In parallel, responsiveness and the minimal clinically important difference should be studied to facilitate sample size calculations in future clinical trials