George Herbert's Lyrics

Originally published in 1968. The main purpose of this book is to demonstrate that George Herbert is one of the great masters of lyric poetry. Stein discusses Herbert's diction, imagery, syntax, and rhythm in light of his organization of the imaginative materials of time and self-consciousness and in light of his development of a rhetoric through which he could master the intimacies of personal failure and (what is far more difficult) express in language convincingly sincere states of positive religious achievement

The House of Death

In The House of Death, Arnold Stein studies the ways in which English poets of the sixteenth and seventeenth centuries imagined their own ends and wrote of the deaths of those they loved or wished to honor. Drawing on a wide range of texts in both poetry and prose, Stein examines the representations, images, and figurative meanings of death from antiquity to the Renaissance. A major premise of the book is that commonplaces, conventions, and the established rules for thinking about death did not prevent writers from discovering the distinctive in it. Eloquent readings of Raleigh, Donne, Herbert, and others capture the poets approaching their own death or confronting the death of others. Marvell's lines on the execution of Charles are paired with his treatment of the dead body of Cromwell; Henry King and John Donne both write of their late wives; Ben Jonson mourns the death of a first son and a first daughter. For purposes of comparison, the governing perspective of the final chapter is modern

Sequence Information Encoded in DNA that May Influence Long-Range Chromatin Structure Correlates with Human Chromosome Functions.

Little is known about the possible function of the bulk of the human genome. We have recently shown that long-range regular oscillation in the motif non-T, A/T, G (VWG) existing at ten-nucleotide multiples influences large-scale nucleosome array formation. In this work, we have determined the locations of all 100 kb regions that are predicted to form distinctive chromatin structures throughout each human chromosome (except Y). Using these data, we found that a significantly greater fraction of 300 kb sequences lacked annotated transcripts in genomic DNA regions ≥300 kb that contained nearly continuous chromatin organizing signals than in control regions. We also found a relationship between the meiotic recombination frequency and the presence of strong VWG chromatin organizing signals. Large (≥300 kb) genomic DNA regions having low average recombination frequency are enriched in chromatin organizing signals. As additional controls, we show using chromosome 1 that the VWG motif signals are not enriched in randomly selected DNA regions having the mean size of the recombination coldspots, and that non-VWG motif sets do not generate signals that are enriched in recombination coldspots. We also show that tandemly repeated alpha satellite DNA contains strong VWG signals for the formation of distinctive nucleosome arrays, consistent with the low recombination activity of centromeres. Our correlations cannot be explained simply by variations in the GC content. Our findings suggest that a specific set of periodic DNA motifs encoded in genomic DNA, which provide signals for chromatin organization, influence human chromosome function

Aspects of large-scale chromatin structures in mouse liver nuclei can be predicted from the DNA sequence

The large amount of non-coding DNA present in mammalian genomes suggests that some of it may play a structural or functional role. We provide evidence that it is possible to predict computationally, from the DNA sequence, loci in mouse liver nuclei that possess distinctive nucleosome arrays. We tested the hypothesis that a 100 kb region of DNA possessing a strong, in-phase, dinucleosome period oscillation in the motif period-10 non-T, A/T, G, should generate a nucleosome array with a nucleosome repeat that is one-half of the dinucleosome oscillation period value, as computed by Fourier analysis of the sequence. Ten loci with short repeats, that would be readily distinguishable from the pervasive bulk repeat, were predicted computationally and then tested experimentally. We estimated experimentally that less than 20% of the chromatin in mouse liver nuclei has a nucleosome repeat length that is 15 bp, or more, shorter than the bulk repeat value of 195 ± bp. All 10 computational predictions were confirmed experimentally with high statistical significance. Nucleosome repeats as short as 172 ± 5 bp were observed for the first time in mouse liver chromatin. These findings may be useful for identifying distinctive chromatin structures computationally from the DNA sequence

Effect of Application and Intensity of Bevacizumab-based Maintenance After Induction Chemotherapy With Bevacizumab for Metastatic Colorectal Cancer: A Meta-analysis

BACKGROUND: The administration and intensity of bevacizumab-based maintenance therapy after induction treatment with bevacizumab is still a matter of debate. Thus, the present meta-analysis and an indirect comparison were performed to clarify these issues. PATIENTS AND METHODS: Trials evaluating a separately defined "maintenance phase," with randomization after the induction phase, were selected. Three trials of maintenance with bevacizumab with or without a fluoropyrimidine (CAIRO3, SAKK 41/06, and AIO KRK 0207) were analyzed regarding the effect on progression-free survival (PFS) and overall survival (OS) of any maintenance therapy compared with observation alone and different maintenance intensities (bevacizumab with or without fluoropyrimidine) compared with observation alone and between each other. RESULTS: Maintenance with bevacizumab with or without fluoropyrimidine after bevacizumab-based induction treatment for 4 to 6 months significantly improved PFS (hazard ratio [HR], 0.57; 95% confidence interval [CI], 0.43-0.75; P = .0004) and showed a trend toward prolonged OS (HR, 0.89; 95% CI, 0.78-1.02; P = .09) compared with observation alone. The effect on PFS increased with the intensity of the maintenance regimen (HR, 0.72; 95% CI, 0.60-0.85 for single-agent bevacizumab vs. HR, 0.45; 95%, CI 0.39-0.51 for combination therapy, both compared to observation alone). In contrast, the HRs for OS remained in the same range. A similarly improved PFS (HR, 0.63; 95% CI, 0.50-0.79) was shown for the more intensive maintenance therapy (bevacizumab and fluoropyrimidine) compared with bevacizumab alone. CONCLUSION: Bevacizumab-based maintenance therapy after induction chemotherapy with bevacizumab significantly improves PFS and showed a trend toward prolonged OS and should thus be considered, in particular, in patients with a response to induction treatment

Long-range oscillation in a periodic DNA sequence motif may influence nucleosome array formation

We have experimentally examined the characteristics of nucleosome array formation in different regions of mouse liver chromatin, and have computationally analyzed the corresponding genomic DNA sequences. We have shown that the mouse adenosine deaminase (MADA) gene locus is packaged into an exceptionally regular nucleosome array with a shortened repeat, consistent with our computational prediction based on the DNA sequence. A survey of the mouse genome indicates that <10% of 70 kb windows possess a nucleosome-ordering signal, consisting of regular long-range oscillations in the period-10 triplet motif non-T, A/T, G (VWG), which is as strong as the signal in the MADA locus. A strong signal in the center of this locus, confirmed by in vitro chromatin assembly experiments, appears to cooperate with weaker, in-phase signals throughout the locus. In contrast, the mouse odorant receptor (MOR) locus, which lacks locus-wide signals, was representative of ∼40% of the mouse genomic DNA surveyed. Within this locus, nucleosome arrays were similar to those of bulk chromatin. Genomic DNA sequences which were computationally similar to MADA or MOR resulted in MADA- or MOR-like nucleosome ladders experimentally. Overall, we provide evidence that computationally predictable information in the DNA sequence may affect nucleosome array formation in animal tissue

Testing and Implementation Progress on the Advanced Photon Source (APS) Linear Accelerator (Linac) High-Power S-band Switching System

An S-band linear accelerator is the source of particles and the front end of the Advanced Photon Source injector. In addition, it supports a low-energy undulator test line (LEUTL) and drives a free-electron laser (FEL). A waveguide-switching and distribution system is now under construction. The system configuration was revised to be consistent with the recent change to electron-only operation. There are now six modulator-klystron subsystems, two of which are being configured to act as hot spares for two S-band transmitters each, so that no single failure will prevent injector operation. The two subsystems are also used to support additional LEUTL capabilities and off-line testing. Design considerations for the waveguide-switching subsystem, topology selection, control and protection provisions, high-power test results, and current status are describedComment: Linac 2000 paper No. THE07 3 pages with 3 figure

The Fairy-Tale Forest: Developing Pedagogical Content Knowledge for teaching Primary School Mathematics in The Scottish Storyline Approach

Chapter 8. This study focuses on the development of mathematical pedagogical content knowl-edge when implementing Storyline as a narrative approach to organising cross-curricu-lar learning for student teachers to become teachers in mathematics for grades 1–7. InStoryline, teachers according to Omand (2014) carefully plan “a ‘line’ of episodes, each of which has carefully designed key questions that encourage and support the learner to contextualise and create the ‘story’, promoting exciting learning” (p. 3). The study uses six semi-structured focus group interviews with a total of 24 first year student teachers. A qualitative analysis, based on the framework of Creswell and Creswell (2018), structures the process of coding. The results contribute to pedagogical content knowledge (cf. Ball, Thames & Phelps, 2008) for teaching primary school mathematics in the following three domains: i) Playing out the Fairy-tale story; ii) Preparing, exploring and performing the tasks; and iii) Learning through a Meta-Storyline. The results show that the students encountered a completely new way of learning mathematics when using Storyline. In summary, although the student teachers report awkwardness when joining the fictional in-role activities, they see the potential for facilitating playful and explorative learning experiences for pupils in primary school.publishedVersio