Water oxygenation and sea bass

The influence of water oxygen concentration on the acid–base balance of sea bass was evaluated. Fish weighing 200–250 g were cultured under different dissolved oxygen concentrations of 64%, 97%, 150% and 250% saturation (92.7, 140.5, 217.5 and 362.7 mmHg respectively) under mild hypoxia, normoxia, mild hyperoxia and high hyperoxia conditions. The results showed that high hyperoxia and mild hypoxia conditions modified some blood parameters significantly when compared with fish held under the normoxia condition, while no differences were shown with respect to the acid–base balance of fish cultured under normoxia and mild hyperoxia conditions. This testifies that the mild hyperoxia condition does not produce physiological disturbances in the acid–base status of sea bass and it could be considered a favourable condition in sea bass land-based farming, mainly in comparison with the mild hypoxia condition, responsible for other physiological problems

Innovative solution for reducing the run-down time of the chipper disc using a brake clamp device

Wood-chippers are widely used machines in the forestry, urban and agricultural sectors. The use of these machines implies various risks for workers, primarily the risk of contact with moving and cutting parts. These machine parts have a high moment of inertia that can lead to entrainment with the cutting components. This risk is particularly high in the case of manually fed chippers. Following cases of injury with wood-chippers and the improvement of the technical standard (Comit\ue9Europ\ue9en de Normalisation-European Norm) EN 13525: 2005 + A2: 2009, this technical note presents the prototype of an innovative system to reduce risks related to the involved moving parts, based on the \u201cbrake caliper\u201d system and electromagnetic clutch for the declutching of the power take-off (PTO). The prototype has demonstrated its potential for reducing the run-down time of the chipper disc (95%) and for reducing the worker\u2019s risk of entanglement and entrainment in the machine\u2019s feed mouth

Innovative solution for reducing the run-down time of the chipper disc using a brake clamp device

Wood-chippers are widely used machines in the forestry, urban and agricultural sectors. The use of these machines implies various risks for workers, primarily the risk of contact with moving and cutting parts. These machine parts have a high moment of inertia that can lead to entrainment with the cutting components. This risk is particularly high in the case of manually fed chippers. Following cases of injury with wood-chippers and the improvement of the technical standard (ComitéEuropéen de Normalisation-European Norm) EN 13525: 2005 + A2: 2009, this technical note presents the prototype of an innovative system to reduce risks related to the involved moving parts, based on the “brake caliper” system and electromagnetic clutch for the declutching of the power take-off (PTO). The prototype has demonstrated its potential for reducing the run-down time of the chipper disc (95%) and for reducing the worker’s risk of entanglement and entrainment in the machine’s feed mouth

Heat stress affects reproductive performance of high producing dairy cows bred in an area of southern Apennines

A 5-year retrospective (from 2008 to 2012) survey was carried out by analyzing data of high producing dairy cows reared in farms located in an area of southern Apennines. The indicators of fertility obtained were related to either season variations or the temperature-humidity index (THI). A significant lower number of conceptions evaluated per month on an annual basis (NCY), i.e., a parameter obtained by subtracting gestation length to the calving date, was found during the summer months; furthermore, this parameter decreased along with THI increase. The number of heats detected varied equally to NCY and represented the main cause of the lower fertility consequent to heat stress (HS). The age at first calving was not significantly affected by either the season or the THI. The mean number of AI/pregnancy in relation to the calving time was significantly affected by the season but it was not related to THI. The number of days open was significantly larger in the animals calved from January to July than from August to December (163±33 vs 123±36 days; P< 0.001); this causes an annual economic loss of several thousand euro in each farm analyzed. In conclusion, HS causes severe economic losses in dairy farms located in southern Apennines that are mainly due to a lower number of heats detected as well as a larger number of days open and semen doses used

METROPOLITAN AGRICULTURE, SOCIO-DEMOGRAPHIC DYNAMICS AND THE FOOD-CITY RELATIONSHIP IN SOUTHERN EUROPE

Peri-urban agriculture is a pivotal issue in the debate on sustainable management of land in metropolitan regions worldwide. Multiple socioeconomic and environmental solutions introduced by new models of peri-urban agriculture are playing an important role in planning and management of fringe land. The recent development of peri-urban agriculture in Southern European cities was supposed to reflect latent, crisis-driven processes of 'coming back to land': new land has been extensively cultivated, and new relations have been created between farmers, communities and territories within peri-urban areas. This study describes some relevant experiences of peri-urban farming in 6 metropolitan regions (Lisbon, Barcelona, Marseille, Rome, Athens, Istanbul) representative of different socioeconomic contexts in Southern Europe, outlining strengths and weaknesses in the use of fringe land for cropping, and evidencing relevant implications for urban sustainability

Analytical Validation of Two Assays for Equine Ceruloplasmin Ferroxidase Activity Assessment

: Ceruloplasmin (Cp) assessment in biological samples exploits the oxidase activity of this enzyme against several substrates, such as p-phenylenediamine (p-P), o-dianisidine (o-D) and, most recently, ammonium iron(II) sulfate (AIS). Once developed in humans, these assays are often used in veterinary medicine without appropriately optimizing in the animal species of interest. In this study, two assays using AIS and o-D as substrates have been compared and validated for Cp oxidase activity assessment in horse's plasma. The optimization of the assays was performed mainly by varying the buffer pH as well as the buffer and the substrate molar concentration. Under the best analytical conditions obtained, the horse blood serum samples were treated with sodium azide, a potent Cp inhibitor. In the o-D assay, 500 µM sodium azide treatment completely inhibits the enzymatic activity of Cp, whereas, using the AIS assay, a residual analytical signal was still present even at the highest (2000 µM) sodium azide concentration. Even though the analytical values obtained from these methods are well correlated, the enzymatic activity values significantly differ when expressed in Units L-1. A disagreement between these assays has also been detected with the Bland-Altman plot, showing a progressive discrepancy between methods with increasing analytical values

Soil conditions under a Fagus sylvatica CONECOFOR stand in Central Italy: an integrated assessment through combined solid phase and solution studies.

As soil solution represents the major phase of soil chemical reactions, its study is a powerful tool for ecological investigations. Soil solution chemical composition gives a realistic idea about the soil chemical components immediately available in the environment, mainly in relation to the soil ecosystem reaction to the disturbance due to acidifying loads. Within the CONECOFOR Program, the monitoring of forest soil conditions was performed in a level II plot (ABR I), under a Fagus sylvatica (European beech) stand, through the study of throughfall and soil solutions collected from depths ranging between the base of the litter layers and 90 cm. To be able to investigate solution contents of nutrients, acidifying agents and DOC throughout the profile, both zero tension and tension lysimeters were used. The first ones were inserted below the organic horizons, while tension lysimeters were placed within the mineral horizons at 15, 25, 55 and 90 cm depth. Sampled solutions were analyzed for Na, K, Ca, Mg, NH4, Cl, F, NO3, SO4, and DOC. The results evidence a clear seasonal pattern, mainly for macronutrients and inorganic N components. Acidic pulses were mostly evident below the organic horizons, in relation to strong nitric N releases from litter; these last were not always immediately neutralized by basic cations. Acid solutions leaving the organic horizons were invariably neutralized in the surface mineral horizons, within 15 cm depth. Temporal patterns of sulphate retention and release suggest that the soil has low retention capability for this anion. Such behaviour can be explained by the composition of the solid phase, where potential anion adsorbants appear strongly linked with organic matter in long residence time complexes. Sulphate and nitrate loading of this soil appear, anyway, to be mostly non-anthropogenic, but rather linked to natural mineralization pulses and, for sulphate, to aeolian solid transport from the south

Fluorescence Spectroscopy for the Diagnosis of Endometritis in the Mare

By exploiting the PMN property to produce high quantities of oxygen peroxide to neutralize pathogens, the oxygen peroxide content of uterine cells was measured to diagnose endometritis. After preliminary in vitro studies in which endometrial cells from slaughtered mares were mixed with leukocytes from peripheral blood, endometrial samples were collected by uterine flushing from mares before insemination. Staining endometrial cells with H2DCF‐DA was combined with hydroethidine to normalize the fluorescence intensity with the cellular content of the sample. Stained cell smears were assumed as the gold standard of endometritis, and based on this assay, the samples were considered positive (C+) and negative (C−) for endometritis. The amount and the turbidity of fluid recovered by uterine flushing were significantly (p < 0.01) higher in C+ than in C−. Moreover, the oxygen peroxide content of the endometrial cells was significantly higher in the C+ than in the C− group (6.31 ± 1.92 vs. 3.12 ± 1.26, p = 0.001). Using the value of 4.4 as the cutoff level of this fluorescence cytology assay, it was found that only one C− sample exceeded the cutoff level (false positives = 7.7%) while three C+ samples showed values below the cutoff level (false negative = 11.5%)

Soil solution chemistry at one mountain beech (Fagus sylvatica L.) CONECOFOR plot, 1999 to 2005

Soil solution monitoring aims to understand various temporal scales of soil processes. The first eight years of observation in ABR1 Level II site have brought significant elements of understanding about the shorter temporal scales. It is suggested that certain solutes, regularly produced by forest floor microbial processes, are transferred to the highly mobile portion of the soil solution by a non linear process, producing irregular pulses and creating a strong high frequency component. Seasonal processes remain nonetheless detectable after simple and rough filtering. A multi-year trend of diminished nitrate mineralization and increased pH of forest floor solutions is possible. It is estimated that much more accurate analysis will be possible in a relatively short time span of further monitoring