Reproductive traits in primiparous sows in relation to feeding level.

To estimate the effect of plane of nutrition and induction of oestrus with 400 IU of pregnant mare serum and 200 IU of human chorion gonadotropin, 113 primiparous Dutch Landrace sows were given 4 or 2.5 kg feed daily from weaning to oestrus. The feed included maize 12, barley 18, soya bean oilmeal 15, wheat bran 15, cassava pellets 13, maize gluten feed 5, lucerne meal 8, citrus pulp 5.5, molasses 5, animal protein 1.1%, minerals and vitamins. The number of sows in oestrus within 21 days of weaning was 69 (61%). Within 7 days of induction of oestrus on day 21, 41 sows were in oestrus (36%). On day 21, 37 (65%) of the better-fed sows were in oestrus and 32 (53%) of the poorer-fed group. The interval from weaning to spontaneous oestrus was 9.1 and 8.2 days, respectively, and ovulation rate 15.2 and 14.8. Size of the sexual organs was not affected by feeding level. Rate of gain of sows during the interval from weaning to oestrus was influenced by feeding level: +4.1 kg and -2.3 kg, respectively, for the sows in oestrus within 21 days of weaning. Sows with induced oestrus shed significantly more ova than did sows in which oestrus was not induced (21.7 and 15.0, respectively). Loss of weight, loss of heart girth, and loss of backfat thickness during previous lactation (absolute and relative) did not differ for sows with or without induction, but was higher for sows with a spontaneous oestrus between 10 and 21 days after weaning than for sows with an oestrus within 10 days after weaning. Ovulation rate was not affected by weight at weaning or at oestrus, weight loss during lactation, gain during the interval from weaning to oestrus or size of the preceeding litter at birth. (Abstract retrieved from CAB Abstracts by CABI’s permission

(Never) Mind your p's and q's: Von Neumann versus Jordan on the Foundations of Quantum Theory

In two papers entitled "On a new foundation [Neue Begr\"undung] of quantum mechanics," Pascual Jordan (1927b,g) presented his version of what came to be known as the Dirac-Jordan statistical transformation theory. As an alternative that avoids the mathematical difficulties facing the approach of Jordan and Paul A. M. Dirac (1927), John von Neumann (1927a) developed the modern Hilbert space formalism of quantum mechanics. In this paper, we focus on Jordan and von Neumann. Central to the formalisms of both are expressions for conditional probabilities of finding some value for one quantity given the value of another. Beyond that Jordan and von Neumann had very different views about the appropriate formulation of problems in quantum mechanics. For Jordan, unable to let go of the analogy to classical mechanics, the solution of such problems required the identication of sets of canonically conjugate variables, i.e., p's and q's. For von Neumann, not constrained by the analogy to classical mechanics, it required only the identication of a maximal set of commuting operators with simultaneous eigenstates. He had no need for p's and q's. Jordan and von Neumann also stated the characteristic new rules for probabilities in quantum mechanics somewhat differently. Jordan (1927b) was the first to state those rules in full generality. Von Neumann (1927a) rephrased them and, in a subsequent paper (von Neumann, 1927b), sought to derive them from more basic considerations. In this paper we reconstruct the central arguments of these 1927 papers by Jordan and von Neumann and of a paper on Jordan's approach by Hilbert, von Neumann, and Nordheim (1928). We highlight those elements in these papers that bring out the gradual loosening of the ties between the new quantum formalism and classical mechanics.Comment: New version. The main difference with the old version is that the introduction has been rewritten. Sec. 1 (pp. 2-12) in the old version has been replaced by Secs. 1.1-1.4 (pp. 2-31) in the new version. The paper has been accepted for publication in European Physical Journal

Online Interactive Teaching Modules Enhance Quantitative Proficiency of Introductory Biology Students

There is widespread agreement within the scientific and education communities that undergraduate biology curricula fall short in providing students with the quantitative and interdisciplinary problem-solving skills they need to obtain a deep understanding of biological phenomena and be prepared fully to contribute to future scientific inquiry. MathBench Biology Modules were designed to address these needs through a series of interactive, Web-based modules that can be used to supplement existing course content across the biological sciences curriculum. The effect of the modules was assessed in an introductory biology course at the University of Maryland. Over the course of the semester, students showed significant increases in quantitative skills that were independent of previous math course work. Students also showed increased comfort with solving quantitative problems, whether or not they ultimately arrived at the correct answer. A survey of spring 2009 graduates indicated that those who had experienced MathBench in their course work had a greater appreciation for the role of mathematics in modern biology than those who had not used MathBench. MathBench modules allow students from diverse educational backgrounds to hone their quantitative skills, preparing them for more complex mathematical approaches in upper-division courses

Genome-wide association and Mendelian randomisation analysis provide insights into the pathogenesis of heart failure

Heart failure (HF) is a leading cause of morbidity and mortality worldwide. A small proportion of HF cases are attributable to monogenic cardiomyopathies and existing genome-wide association studies (GWAS) have yielded only limited insights, leaving the observed heritability of HF largely unexplained. We report results from a GWAS meta-analysis of HF comprising 47,309 cases and 930,014 controls. Twelve independent variants at 11 genomic loci are associated with HF, all of which demonstrate one or more associations with coronary artery disease (CAD), atrial fibrillation, or reduced left ventricular function, suggesting shared genetic aetiology. Functional analysis of non-CAD-associated loci implicate genes involved in cardiac development (MYOZ1, SYNPO2L), protein homoeostasis (BAG3), and cellular senescence (CDKN1A). Mendelian randomisation analysis supports causal roles for several HF risk factors, and demonstrates CAD-independent effects for atrial fibrillation, body mass index, and hypertension. These findings extend our knowledge of the pathways underlying HF and may inform new therapeutic strategies

Hyperglycaemic conditions perturb mouse oocyte in vitro developmental competence via beta-O-linked glycosylation of Heat shock protein 90

STUDY QUESTION What is the effect of beta-O-linked glycosylation (O-GlcNAcylation) on specific proteins in the cumulus-oocyte complex (COC) under hyperglycaemic conditions? SUMMARY ANSWER Heat shock protein 90 (HSP90) was identified and confirmed as being O-GlcNAcylated in mouse COCs under hyperglycaemic conditions (modelled using glucosamine), causing detrimental outcomes for embryo development. WHAT IS KNOWN ALREADY O-GlcNAcylation of proteins occurs as a result of increased activity of the hexosamine biosynthesis pathway, which provides substrates for cumulus matrix production during COC maturation, and also for O-GlcNAcylation. COCs matured under hyperglycaemic conditions have decreased developmental competence, mediated at least in part through the mechanism of increased O-GlcNAcylation. STUDY DESIGN, SIZE, DURATION This study was designed to examine the effect of hyperglycaemic conditions (using the hyperglycaemic mimetic, glucosamine) on O-GlcNAc levels in the mouse COC, and furthermore to identify potential candidate proteins which are targets of this modification, and their roles in oocyte maturation. PARTICIPANTS/MATERIALS, SETTING, METHODS COCs from 21-day-old superovulated CBA × C57BL6 F1 hybrid female mice were matured in vitro (IVM). Levels of O-GlcNAcylated proteins, HSP90 and O-GlcNAc transferase (OGT, the enzyme responsible for O-GlcNAcylation) in COCs were measured using western blot, and localization observed using immunocytochemistry. For glycosylated HSP90 levels, and to test OGT-HSP90 interaction, immunoprecipitation was performed prior to western blotting. Embryo development was assessed using in vitro fertilization and embryo culture post-maturation. MAIN RESULTS AND THE ROLE OF CHANCE Addition of the hyperglycaemic mimetic glucosamine to IVM medium for mouse COCs increased detectable O-GlcNAcylated protein levels (by western blot and immunocytochemistry), and this effect was reversed using an OGT inhibitor (P < 0.05). HSP90 was identified as a target of O-GlcNAcylation in the COC, and inhibition of HSP90 during IVM reversed glucosamine-induced decreases in oocyte developmental competence (P < 0.05). We also demonstrated the novel finding of an association between HSP90 and OGT in COCs, suggesting a possible client–chaperone relationship. LIMITATIONS, REASONS FOR CAUTION In vitro maturation of COCs was used so that treatment time could be limited to the 17 h of maturation prior to ovulation. Additionally, glucosamine, a hyperglycaemic mimetic, was used because it specifically activates the hexosamine pathway which provides the O-GlcNAc moieties. The results in this study should be confirmed using in vivo models of hyperglycaemia and different HSP90 inhibitors. WIDER IMPLICATIONS OF THE FINDINGS This study leads to a new understanding of how diabetes influences oocyte competence and provides insight into possible therapeutic interventions based on inhibiting HSP90 to improve oocyte quality.L.A. Frank, M.L. Sutton-McDowall, H.M. Brown, D.L. Russell, R.B. Gilchrist, and J.G. Thompso

Analysis of shared heritability in common disorders of the brain

Paroxysmal Cerebral Disorder