Ankieta na temat: Czy dekret prasowy obowiązuje?

Digitalizacja i deponowanie archiwalnych zeszytów RPEiS sfinansowane przez MNiSW w ramach realizacji umowy nr 541/P-DUN/201

Creating patient-specific Finite Element Models with a Simple Mesh Morpher

The paper presents a simple 3D finite element mesh morpher aimed at creation of patient-specific models of human body parts. These models are to be used in realistic simulation of magneto- and electrotherapeutic treatment. The presented morpher uses simple algorithm of guided stretching which needs only a few measurements of patients body, but it may deform some finite elements. A public domain code Stellar is used to fix these problems

Ankieta na temat: Czy dekret prasowy obowiązuje?

Digitalizacja i deponowanie archiwalnych zeszytów RPEiS sfinansowane przez MNiSW w ramach realizacji umowy nr 541/P-DUN/201

Influence of elevated temperature on bovine oviduct epithelial cells (BOECs)

<div><p>The aim of this study was to evaluate the influence of elevated temperature on bovine oviduct epithelial cells (BOECs), based on the expression and localization of both heat shock protein 70 (HSP70), responsible for the cellular defence mechanism, and oviduct specific glycoprotein 1 (OVGP1) which is the most important embryotrophic protein. BOECs were cultured alone and co-cultured with cattle embryos at control (38.5°C) and elevated temperature (41°C) for 168 h. The elevated temperature had no effect on the viability of BOECs but exerted a negative effect on embryo development. The elevated temperature increased the expression of HSP70 and decreased the expression of OVGP1 at both mRNA and protein levels in BOECs cultured alone and those co-cultured with embryos. However, the presence of embryos limited the decrease in OVGP1 expression in BOECs at elevated temperature but did not alter the expression of HSP70. These results demonstrate for the first time the influence of elevated temperature on BOECs, consequently providing insights into the interactions between the embryo and the oviduct at elevated temperature.</p></div

The expression of ovgp1 mRNA in BOECs cultured alone and co-cultured with cattle embryos at control (38.5°C) and elevated (41°C) temperatures for 168 h post fertilization.

<p>Data presented as Mean±SEM. Bars with different letters denote statistical differences at P<0.01.</p

Fluorescent analysis of HSP70 in BOECs cultured alone and co-cultured with cattle embryos at control (38.5°C) and elevated (41°C) temperatures for 168 h post fertilization.

<p>A) Representative images of immunofluorescent staining of HSP70 in BOECs cultured alone at control (38.5°C) (I) and elevated (41°C) (II) temperatures, or co-cultured with cattle embryos at control (38.5°C) (III) and elevated (41°C) (IV) temperatures. Nuclei were counterstained with Hoechst 33342 (blue); HSP70 localization was determined using antibody against HSP70 and Alexa Fluor 488-conjugated secondary antibody (green). B) The IOD values of HSP70-related green fluorescence were measured using the MicroImage Olympus Optical analysis system. The data are presented as Mean±SEM. Bars with different letters denote statistical differences at P<0.05.</p

Fluorescent analysis of OVGP1 in BOECs cultured alone and co-cultured with cattle embryos at control (38.5°C) and elevated (41°C) temperatures for 168 h post fertilization.

<p>A) Representative images of immunofluorescent staining of OVGP1 in BOECs cultured alone at control (38.5°C) (I) and elevated (41°C) (II) temperatures, or co-cultured with cattle embryos at control (38.5°C) (III) and elevated (41°C) (IV) temperatures. Nuclei were counterstained with Hoechst 33342 (blue); OVGP1 localization was determined using antibody against OVGP1 and Alexa Fluor 488-conjugated secondary antibody (green). B) The IOD values of OVGP1-related green fluorescence were measured using the MicroImage Olympus Optical analysis system. The data are presented as Mean±SEM. Bars with different letters denote statistical differences at P<0.05.</p

Oligonucleotide primers used in Real Time PCR assays.

<p>Oligonucleotide primers used in Real Time PCR assays.</p

Analysis of development of cattle embryos co-cultured with BOECs at control (38.5°C) and elevated (41°C) temperatures for 168 h post fertilization.

<p>Data presented as Mean±SEM. Bars with different letters denote statistical differences at P<0.01.</p

Analysis of HSP70 in BOECs cultured alone and co-cultured with cattle embryos at control (38.5°C) and elevated (41°C) temperatures for 168 h post fertilization.

<p>A) Western blots of HSP70 protein. B) Densitometric quantification of HSP70 protein expression. Data presented as Mean±SEM. Bars with different letters denote statistical differences at P<0.05.</p