202 research outputs found
Polycomb RING1A- and RING1B-dependent histone H2A monoubiquitylation at pericentromeric regions promotes S-phase progression
The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, particularly without induced replicative stress.We have used a cellularmodel of conditionally inactive polycomb E3 ligases (RING1A and RING1B), which monoubiquitylate lysine 119 of histone H2A (H2AK119Ub), to examine DNA replication in unperturbed cells. We identify slow elongation and fork stalling during DNA replication that is associated with the accumulation of mid and late S-phase cells. Signs of replicative stress and colocalisation of double-strand breaks with chromocenters, the sites of coalesced pericentromeric heterocromatic (PCH) domains, were enriched in cells at mid S-phase, the stage at which PCH is replicated. Altered replication was rescued by targeted monoubiquitylation of PCH through methyl- CpG binding domain protein 1. The acute senescence associated with the depletion of RING1 proteins, which is mediated by p21 (also known as CDKN1A) upregulation, could be uncoupled from a response to DNA damage. These findings link cell proliferation and the polycomb proteins RING1A and RING1B to S-phase progression through a specific function in PCH replication.Ministerio de Economı́a y Competitividad BFU2010-18146, SAF2013-47997, BFU2013-42918-
Cohesion and adhesion properties of modified water glass with colloidal solutions of ZnO
The article discusses the issue of the influence of colloidal solutions of nanoparticles of ZnO obtained using original method on physical and chemical properties (cohesions, adhesion, wettability) of water glass. Adhesion and cohesion strength has been determined on the basis of own methodology of measurement using a special apparatus. The results of the above measurements have been supplemented with selected measurements of quartz wettability by the modified binder. Also, exemplary results have been presented of tests involving basic properties of moulding sans containing an addition of the aforementioned binder. In conclusions, the influence of binder modification on the properties of moulding sands have been presented
Generalized Spectral Signatures of Electron Fractionalization in Quasi-One and -Two Dimensional Molybdenum Bronzes and Superconducting Cuprates
We establish the quasi-one-dimensional Li purple bronze as a photoemission
paradigm of Luttinger liquid behavior. We also show that generalized signatures
of electron fractionalization are present in the angle resolved photoemission
spectra for quasi-two-dimensional purple bronzes and certain cuprates. An
important component of our analysis for the quasi-two-dimensional systems is
the proposal of a ``melted holon'' scenario for the k-independent background
that accompanies but does not interact with the peaks that disperse to define
the Fermi surface.Comment: 7 pages, 8 figure
Fully Gapped Single-Particle Excitations in the Lightly Doped Cuprates
The low-energy excitations of the lightly doped cuprates were studied by
angle-resolved photoemission spectroscopy. A finite gap was measured over the
entire Brillouin zone, including along the d_{x^2 - y^2} nodal line. This
effect was observed to be generic to the normal states of numerous cuprates,
including hole-doped La_{2-x}Sr_{x}CuO_{4} and Ca_{2-x}Na_{x}CuO_{2}Cl_{2} and
electron-doped Nd_{2-x}Ce_{x}CuO_{4}. In all compounds, the gap appears to
close with increasing carrier doping. We consider various scenarios to explain
our results, including the possible effects of chemical disorder, electronic
inhomogeneity, and a competing phase.Comment: To appear in Phys. Rev.
Differential effects of TRPV1 receptor ligands against nicotine-induced depression-like behaviors
Increased susceptibility to cardiovascular effects of dihydrocapcaicin in resuscitated rats. Cardiovascular effects of dihydrocapsaicin
<p>Abstract</p> <p>Background</p> <p>Survivors of a cardiac arrest often have persistent cardiovascular derangements following cardiopulmonary resuscitation including decreased cardiac output, arrhythmias and morphological myocardial damage. These cardiovascular derangements may lead to an increased susceptibility towards the external and internal environment of the cardiovascular system as compared to the healthy situation.</p> <p>Methods</p> <p>Here we tested the hypothesis that the cardiovascular system in healthy rats and rats resuscitated from a cardiac arrest may be differentially affected by a transient receptor potential vanilloid type 1 agonist, by continuous intravenous infusion of dihydrocapsaicin (DHC).</p> <p>Results</p> <p>Compared to baseline, infusion of DHC caused an initial increase in mean arterial blood pressure in both healthy and resuscitated rats of 25% and 10%, respectively. Also, we observed an initial response of tachycardia in both healthy and resuscitated rats of 30% and 20%, respectively. Then, at high levels of DHC infusion (> 2.0 mg/kg/hr) we observed two single episodes of transient bradycardia and hypotension in 33% of the healthy rats, which was consistent with a TRPV1 agonist induced Bezold-Jarisch reflex. In contrast, in resuscitated rats we observed multiple episodes of bradycardia/hypotension in 100% of the rats and at a dose of DHC of 0.65 mg/kg/hr. Notably, this DHC effect could be completely blocked in the resuscitated rats by pre-treatment with atropine, a muscarinic acetylcholine antagonist.</p> <p>Conclusions</p> <p>Our results indicate that the susceptibility of the rats towards TRPV1 agonist induced Bezold-Jarisch reflex is increased in those resuscitated from cardiac arrest compared to the healthy situation.</p
Photoemission signature of momentum-dependent hybridization in CeCoIn5
Hybridization between f electrons and conduction bands (c-f hybridization) is a driving force for many unusual phenomena. To provide insight into it, systematic studies of CeCoIn 5 heavy fermion superconductor have been performed by angle-resolved photoemission spectroscopy (ARPES) in a large angular range at temperature of T = 6 K. The used photon energy of 122 eV corresponds to Ce 4d-4f resonance. Calculations carried out with the relativistic multiple scattering Korringa-Kohn-Rostoker method and one-step model of photoemission yielded realistic simulation of the ARPES spectra, indicating that Ce-In surface termination prevails. Surface states, which have been identified in the calculations, contribute significantly to the spectra. Effects of the hybridization strongly depend on wave vector. They include a dispersion of heavy electrons and bands gaining f-electron character when approaching Fermi energy. We have also observed a considerable variation of f-electron spectral weight at EF , which is normally determined by both matrix element effects and wave vector dependent c-f hybridization. Fermi surface scans covering a few Brillouin zones revealed large matrix element effects. A symmetrization of experimental Fermi surface, which reduces matrix element contribution, yielded a specific variation of 4f-electron enhanced spectral intensity at EF around Gamma barre and M barre points. Tight-binding approximation calculations for Ce-In plane provided the same universal distribution of 4f-electron density for a range of values of the parameters used in the model
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