Constraints on the injection energy of positrons in the Galactic centre region

Recent observations of the 511 keV positron-electron annihilation line in the Galactic centre region by the INTEGRAL/SPI spectrometer have stirred up new speculations about the origin of the large corresponding positron injection rate. Beyond astrophysical candidates, new mechanisms have been put forward. We focus on the annihilation of light dark matter particles and review the various gamma-ray radiation components related to such a source of mono-energetic positrons in addition to the 511 keV line itself. We study the influence of the degree of ionisation of the bulge on this radiation, and its possible effects on the observational constraints on the mass of the hypothetical light dark matter particle or the injection energy of a mono-energetic source of positrons in general.Comment: 4 pages, 7 figures, 1 table. Accepted for publication in the proceedings of the 6th INTEGRAL Workshop on the Obscured Universe (ESA SP-622). 2-8 July 2006, Moscow, Russi

Soft gamma-ray galactic ridge emission as unveiled by SPI aboard INTEGRAL

The origin of the soft gamma-ray (200 keV - 1 MeV) galactic ridge emission is one of the long-standing mysteries in the field of high-energy astrophysics. Population studies at lower energies have shown that emission from accreting compact objects gradually recedes in this domain, leaving place to another source of gamma-ray emission that is characterised by a hard power-law spectrum extending from 100 keV up to 100 MeV The nature of this hard component has remained so far elusive, partly due to the lack of sufficiently sensitive imaging telescopes that would be able to unveil the spatial distribution of the emission. The SPI telescope aboard INTEGRAL allows now for the first time the simultaneous imaging of diffuse and point-like emission in the soft gamma-ray regime. We present here all-sky images of the soft gamma-ray continuum emission that clearly reveal the morphology of the different emission components. We discuss the implications of our results on the nature of underlying emission processes and we put our results in perspective of GLAST studies of diffuse galactic continuum emission

Imaging the Gamma-Ray Sky with SPI aboard INTEGRAL

The spectrometer SPI on INTEGRAL allows for the first time simultaneous imaging of diffuse and point-like emission in the hard X-ray and soft gamma-ray regime. To fully exploit the capabilities of the instrument, we implemented the MREM image deconvolution algorithm, initially developed for COMPTEL data analysis, to SPI data analysis. We present the performances of the algorithm by means of simulations and apply it to data accumulated during the first 2 mission years of INTEGRAL. Skymaps are presented for the 1809 keV gamma-ray line, attributed to the radioactive decay of 26Al, and for continuum energy bands, covering the range 20 keV - 3 MeV. The 1809 keV map indicates that emission is clearly detected by SPI from the inner Galactic radian and from the Cygnus region. The continuum maps reveal the transition between a point-source dominated hard X-ray sky to a diffuse emission dominated soft gamma-ray sky. From the skymaps, we extract a Galactic ridge emission spectrum that matches well SPI results obtained by model fitting. By comparing our spectrum with the cumulative flux measured by IBIS from point sources, we find indications for the existence of an unresolved or diffuse emission component above ~100 keV.Comment: 12 pages, 7 figure

In situ three-dimensional monitoring of collagen fibrillogenesis using SHG microscopy.

International audienceWe implemented in situ time-lapse Second Harmonic Generation (SHG) microscopy to monitor the three-dimensional (3D) self-assembly of collagen in solution. As a proof of concept, we tuned the kinetics of fibril formation by varying the pH and measured the subsequent exponential increase of fibril volume density in SHG images. We obtained significantly different time constants at pH = 6.5 ± 0.3 and at pH = 7.5 ± 0.3. Moreover, we showed that we could focus on the growth of a single isolated collagen fibril because SHG microscopy is sensitive to well-organized fibrils with diameter below the optical resolution. This work illustrates the potential of SHG microscopy for the rational design and characterization of collagen-based biomaterials

Fibrillogenesis from nanosurfaces: multiphoton imaging and stereological analysis of collagen 3D self-assembly dynamics

International audienceThe assembly of proteins into fibrillar structures is an important process that concerns different biological contexts, including molecular medicine and functional biomaterials. Engineering of hybrid biomaterials can advantageously provide synergetic interactions of the biopolymers with an inorganic component to ensure specific supramolecular organization and dynamics. To this aim, we designed hybrid systems associating collagen and surface-functionalized silica particles and we built a new strategy to investigate fibrillogenesis processes in such multicomponents systems, working at the crossroads of chemistry, physics and mathematics. The self-assembly process was investigated by bimodal multiphoton imaging coupling second harmonic generation (SHG) and 2 photon excited fluorescence (2PEF). The in-depth spatial characterization of the system was further achieved using the three-dimensional analysis of the SHG/2PEF data via mathematical morphology processing. Quantitation of collagen distribution around particles offers strong evidence that the chemically induced confinement of the protein on the silica nanosurfaces has a key influence on the spatial extension of fibrillogenesis. This new approach is unique in the information it can provide on 3D dynamic hybrid systems and may be extended to other associations of fibrillar molecules with optically responsive nano-objects

Ex vivo multiscale quantitation of skin biomechanics in wild-type and genetically-modified mice using multiphoton microscopy

International audienceSoft connective tissues such as skin, tendon or cornea are made of about 90% of extracellular matrix proteins, fibrillar collagens being the major components. Decreased or aberrant collagen synthesis generally results in defective tissue mechanical properties as the classic form of Elhers-Danlos syndrome (cEDS). This connective tissue disorder is caused by mutations in collagen V genes and is mainly characterized by skin hyperextensibility. To investigate the relationship between the microstructure of normal and diseased skins and their macroscopic mechanical properties, we imaged and quantified the microstructure of dermis of ex vivo murine skin biopsies during uniaxial mechanical assay using multiphoton microscopy. We used two genetically-modified mouse lines for collagen V: a mouse model for cEDS harboring a Col5a2 deletion (a.k.a. pN allele) and the transgenic K14-COL5A1 mice which overexpress the human COL5A1 gene in skin. We showed that in normal skin, the collagen fibers continuously align with stretch, generating the observed increase in mechanical stress. Moreover, dermis from both transgenic lines exhibited altered collagen reorganization upon traction, which could be linked to microstructural modifications. These findings show that our multiscale approach provides new crucial information on the biomechanics of dermis that can be extended to all collagen-rich soft tissues

Determination of collagen fiber orientation in histological slides using Mueller microscopy and validation by second harmonic generation imaging.

International audienceWe studied the azimuthal orientations of collagen fibers in histological slides of uterine cervical tissue by two different microscopy techniques, namely Mueller polarimetry (MP) and Second Harmonic Generation (SHG). SHG provides direct visualization of the fibers with high specificity, which orientations is then obtained by suitable image processing. MP provides images of retardation (among other polarimetric parameters) due to the optical anisotropy of the fibers, which is enhanced by Picrosirius Red staining. The fiber orientations are then assumed to be those of the retardation slow axes. The two methods, though fully different from each other, provide quite similar maps of average fiber orientations. Overall, our results confirm that MP microscopy provides reliable images of dominant fiber orientations at a much lower cost that SHG, which remains the "gold standard" for specific imaging of collagen fibers using optical microscopy