27 research outputs found
Study of X-ray photoionized Fe plasma and comparisons with astrophysical modeling codes
Radiative properties of stellar plasmas and open challenges
The lifetime of solar-like stars, the envelope structure of more massive
stars, and stellar acoustic frequencies largely depend on the radiative
properties of the stellar plasma. Up to now, these complex quantities have been
estimated only theoretically. The development of the powerful tools of helio-
and astero- seismology has made it possible to gain insights on the interiors
of stars. Consequently, increased emphasis is now placed on knowledge of the
monochromatic opacity coefficients. Here we review how these radiative
properties play a role, and where they are most important. We then concentrate
specifically on the envelopes of Cephei variable stars. We discuss the
dispersion of eight different theoretical estimates of the monochromatic
opacity spectrum and the challenges we need to face to check these calculations
experimentally.Comment: 6 pages, 5 figures, in press (conference HEDLA 2010
hsp65 PCR-restriction enzyme analysis (PRA) for identification of mycobacteria in the clinical laboratory
Non-local-thermodynamical-equilibrium effects in the x-ray emission of radiatively heated materials of different atomic numbers
Reforestation and Topography Affect Montane Soil Properties, Nitrogen Pools, and Nitrogen Transformations in Hawaii
Histone deacetylase 5 regulates interleukin 6 secretion and insulin action in skeletal muscle.
Objective: Physical exercise training is associated with increased glucose uptake in skeletal muscle and improved glycemic control. HDAC5, a class IIa histone deacetylase, has been shown to regulate transcription of the insulin-responsive glucose transporter GLUT4 in cultured muscle cells. In this study, we analyzed the contribution of HDAC5 to the transcriptional network in muscle and the beneficial effect of muscle contraction and regular exercise on glucose metabolism. Methods: HDAC5 knockout mice (KO) and wild-type (WT) littermates were trained for 8 weeks on treadmills, metabolically phenotyped, and compared to sedentary controls. Hdac5-deficient skeletal muscle and cultured Hdac5-knockdown (KD) C2C12 myotubes were utilized for studies of gene expression and glucose metabolism. Chromatin immunoprecipitation (ChIP) studies were conducted to analyze Il6 promoter activity using H3K9ac and HDAC5 antibodies. Results: Global transcriptome analysis of Hdac5 KO gastrocnemius muscle demonstrated activation of the IL-6 signaling pathway. Accordingly, knockdown of Hdac5 in C2C12 myotubes led to higher expression and secretion of IL-6 with enhanced insulin-stimulated activation of AKT that was reversed by Il6 knockdown. Moreover, Hdac5-deficient myotubes exhibited enhanced glucose uptake, glycogen synthesis, and elevated expression levels of the glucose transporter GLUT4. Transcription of Il6 was further enhanced by electrical pulse stimulation in Hdac5-deficient C2C12 myotubes. ChIP identified a ∼1 kb fragment of the Il6 promoter that interacts with HDAC5 and demonstrated increased activation-associated histone marker AcH3K9 in Hdac5-deficient muscle cells. Exercise intervention of HDAC5 KO mice resulted in improved systemic glucose tolerance as compared to WT controls. Conclusions: We identified HDAC5 as a negative epigenetic regulator of IL-6 synthesis and release in skeletal muscle. HDAC5 may exert beneficial effects through two different mechanisms, transcriptional control of genes required for glucose disposal and utilization, and HDAC5-dependent IL-6 signaling cross-talk to improve glucose uptake in muscle in response to exercise