The Impact of Feral Honey Bees on Endangered Seabirds in the Hawaiian Islands

The ʻaʻo (Puffinus newellii; Newell’s shearwater) and ʻuaʻu (Pterodroma sandwichensis; Hawaiian petrel) are both endangered seabirds endemic to the Hawaiian Archipelago. For the last decade, multiple colonies on the islands of Kauaʻi and Lānaʻi have been the focus of intensive management (particularly predator control) and monitoring programs. Management has been effective at reducing the number of introduced mammalian predators recorded within colonies with a resultant increase in reproductive success rates for both seabird species. However, in recent years a new threat has emerged—feral honey bees (Apis mellifera; bees)—which were deliberately introduced to the Hawaiian Islands starting in 1857 for beekeeping. Between 2011 and 2021, 17 bee swarms were recorded at seabird burrows on both islands combined, consisting of 14 Hawaiian petrel burrows and 3 Newell’s shearwater burrows. Ten (58.8%) of these incidents resulted in the bees taking over the burrow and building a hive inside. Of the 10 takeovers, all resulted in a failed breeding season in that year, and 6 resulted in a seabird mortality (including 2 instances where both adults of a breeding pair were killed). Furthermore, for burrows where bee takeovers occurred, only 30.0% of pairs reinitiated breeding in the following year, despite the hive being removed as soon as it was discovered. An analysis of microhabitat data revealed that bee swarms were more likely at burrows located at the base of cliff walls. We discuss management strategies that can be implemented, including reactive methods such as bee vacuums and proactive methods such as bee swarm traps using pheromones, to mitigate species conservation threats. Addressing this issue is critical, as climate change may increase the likelihood of these events occurring in the future

Mitigating Bighorn Sheep–Vehicle Collisions and Habitat Fragmentation with Overpasses and Adaptive Mitigation

As transportation infrastructure expands to accommodate increasing human population growth, wildlife–vehicle conflicts (WVCs) are a growing concern for motorist safety and wildlife populations. In the case of large ungulates, minimal information exists on successful mitigation of WVCs involving bighorn sheep (Ovis canadensis) and habitat fragmentation. Too address this void, we evaluated the effectiveness of 3 new wildlife overpasses, 3 culverts, 2 bridges, and ungulate exclusionary fencing as potential desert bighorn sheep (O. c. nelsoni; sheep) crossing opportunities along US Highway 93 in Arizona, USA. We evaluated sheep movements using global positioning system (GPS) radio-transmitter collars and remote cameras for 4 years from March 2011 to March 2015 and sheep–vehicle collision data collected from 2011 to 2020. Although GPS determined pre- and post-mitigation passage rates of sheep that crossed US Highway 93 were initially similar, they increased every year and were on average 217% higher following inclusion of wildlife crossings and by year 4 had ultimately increased 633% from pre-construction rates. Cameras recorded 6,936 crossings by a dozen wildlife species with sheep accounting for 95% of all crossings. Sheep used the 3 overpasses (90% of all sheep crossings) disproportionately more than the 3 culverts and 2 underpasses (10% of all sheep crossings) in the same area, and use of the 3 overpasses increased 905% in the first year. Sheep initially used the 30-m-wide overpasses at 83% and 175% higher passage rates than 2 15-m-wide structures; however, by year 4 passage rates were similar across overpasses. From February 2011 through February 2020, we documented 0.8 sheep–vehicle collisions/year for an overall 93.3% reduction from the 12 collisions per year previously documented. Most of the collisions occurred immediately following completion of the project and gradually reduced as sheep access points were identified and addressed through an adaptive mitigation process to iteratively improve success. Overpasses appear to be the preferred wildlife crossing type for sheep and when properly located and linked with ungulate exclusion fencing successfully reduced collisions and habitat fragmentation. These findings add to our knowledge base of effective roadway mitigation for different species. Long-term monitoring informs species learning curves, preference of wildlife crossing structure type, and adaptive mitigation opportunities to increase effectiveness of mitigation measures on current and future projects

Temperature-dependent release of ATP from human erythrocytes: Mechanism for the control of local tissue perfusion

Copyright @ 2012 The AuthorsThis article has been made available through the Brunel Open Access Publishing Fund.Human limb muscle and skin blood flow increases significantly with elevations in temperature, possibly through physiological processes that involve temperature-sensitive regulatory mechanisms. Here we tested the hypothesis that the release of the vasodilator ATP from human erythrocytes is sensitive to physiological increases in temperature both in vitro and in vivo, and examined potential channel/transporters involved. To investigate the source of ATP release, whole blood, red blood cells (RBCs), plasma and serum were heated in vitro to 33, 36, 39 and 42°C. In vitro heating augmented plasma or ‘bathing solution’ ATP in whole blood and RBC samples, but not in either isolated plasma or serum samples. Heat-induced ATP release was blocked by niflumic acid and glibenclamide, but was not affected by inhibitors of nucleoside transport or anion exchange. Heating blood to 42°C enhanced (P < 0.05) membrane protein abundance of cystic fibrosis transmembrane conductance regulator (CFTR) in RBCs. In a parallel in vivo study in humans exposed to whole-body heating at rest and during exercise, increases in muscle temperature from 35 to 40°C correlated strongly with elevations in arterial plasma ATP (r2 = 0.91; P = 0.0001), but not with femoral venous plasma ATP (r2 = 0.61; P = 0.14). In vitro, however, the increase in ATP release from RBCs was similar in arterial and venous samples heated to 39°C. Our findings demonstrate that erythrocyte ATP release is sensitive to physiological increases in temperature, possibly via activation of CFTR-like channels, and suggest that temperature-dependent release of ATP from erythrocytes might be an important mechanism regulating human limb muscle and skin perfusion in conditions that alter blood and tissue temperature.This article is made available through the Brunel Open Access Publishing Fund

Immune Response to the West Nile Virus in Aged Non-Human Primates

Background: Risk of encephalitis from West Nile virus (WNV) infection increases dramatically with age. Understanding the basis of this susceptibility requires development of suitable animal models. Here, we investigated the immune response to WNV in old non-human primates. Methodology/Principal Findings: We investigated clinical, immunological and virological correlates of WNV infection in aging non-human primates. Aged (17-30yrs) and adult (6-9yrs) Rhesus macaques (RM) were challenged with WNV in the presence or the absence of the mosquito salivary gland extract (SGE) to approximate natural infection. None of the 26 animals exhibited clinical signs of the disease. Quantitative PCR suggested discrete and short-lived viremia, but infectious virus was never isolated. There was markedly increased, age-independent, proliferation of CD3- non-B cells, followed by Bcell proliferation, which correlated to the loss of detectable WNV genomes. Moreover, animals primed with mosquito salivary gland extract exhibited reduced circulating WNV RNA. While we found the expected age-associated reduction in T cell proliferation, adaptive immunity did not correlate with infection outcome. That was further confirmed in a cohort of thymectomized and/or CD8 T-cell depleted Cynomolgus macaques (CM; N = 15), who also failed to develop WNV disease. Conclusions/significance: Results are consistent with strong and age-independent innate resistance of macaques against WNV challenge. This animal model is therefore not suitable for vaccine and therapeutic testing against WNV. However, understanding the basis of their innate resistance against WNV in macaques could provide helpful clues to improve anti- WNV protection of older adults. © 2010 Wertheimer et al

Dark Spots on Mercury: A Distinctive Low-Reflectance Material and Its Relation to Hollows

Orbital images acquired by the Mercury, Surface, Space Environment, Geochemistry, and Ranging (MESSENGER) spacecraft reveal a distinctive low-reflectance material on the surface of Mercury. Such material occurs in small, isolated, and thin surficial units. We term these features “dark spots.” Dark spots have the lowest average reflectance yet documented on the planet. In every case observed at sufficiently high resolution, dark spots feature hollows at their centers. Not all hollows, however, are surrounded by a dark spot. Dark spots have been found on low-reflectance smooth plains, intercrater plains, heavily cratered terrain, and impact craters at almost all longitudes on Mercury, but they have not been documented on high-reflectance smooth plains material. Dark spots are one of the youngest endogenic features on Mercury, and some postdate craters with distinctive rays. Sulfides may be the phase responsible for the low albedo of dark spot material. We propose that dark spots form during the initial stages of hollow formation, perhaps in a manner similar to intense outgassing events that feature exit velocities in excess of 100 m/s. Such outgassing could contemporaneously produce a depression that constitutes an embryonic hollow. Under this scenario, dark spot material is subsequently removed or modified by regolith gardening or other surface processes on time scales shorter than the lifetime of the central hollow

The silver bullet that wasn’t: Rapid agronomic weed adaptations to glyphosate in North America

The rapid adoption of glyphosate-resistant crops at the end of the 20th century caused a simplification of weed management that relied heavily on glyphosate for weed control. However, the effectiveness of glyphosate has diminished. A greater understanding of trends related to glyphosate use will shed new light on weed adaptation to a product that transformed global agriculture. Objectives were to (1) quantify the change in weed control efficacy from postemergence (POST) glyphosate use on troublesome weeds in corn and soybean and (2) determine the extent to which glyphosate preceded by a preemergence (PRE) improved the efficacy and consistency of weed control compared to glyphosate alone. Herbicide evaluation trials from 24 institutions across the United States of America and Canada from 1996 to 2021 were compiled into a single database. Two subsets were created; one with glyphosate applied POST, and the other with a PRE herbicide followed by glyphosate applied POST. Within each subset, mean and variance of control ratings for seven problem weed species were regressed over time for nine US states and one Canadian province. Mean control with POST glyphosate alone decreased over time while variability in control increased. Glyphosate preceded by a labeled PRE herbicide showed little change in mean control or variability in control over time. These results illustrate the rapid adaptation of agronomically important weed species to the paradigm-shifting product glyphosate. Including more diversity in weed management systems is essential to slowing weed adaptation and prolonging the usefulness of existing and future technologies

Maize Cultivar Performance under Diverse Organic Production Systems

Maize (Zea mays L.) performance can vary widely between different production systems. The need for high-performing hybrids for organic systems with wide adaptation to various macroenvironments is becoming increasingly important. The goal of this study was to characterize inbred lines developed by distinct breeding programs for their combining ability and hybrid yield performance across diverse organic environments. Parent lines were selected from five different breeding programs to give a sample of publically available germplasm with potential for use in organic production systems with expired plant variety protection (Ex-PVP) and current commercial inbreds as benchmarks. A North Carolina Design II mating design was used to produce all possible cross combinations between seven lines designated as males and seven lines designated as females. A significantly positive general combining ability for the female inbred UHF134 suggests that it performs well in hybrid combination. Significant general combining ability was not observed for any male inbred line in this study. Several significantly positive specific combining abilities suggest that nonadditive genetic effects play an important role in determining yield in this germplasm. Further analysis revealed that hybrids containing either an Ex-PVP line or a commercial inbred line were on average superior to hybrids containing only inbreds developed by the cooperators of this study. This demonstrates the utility of testing inbreds from diverse sources when developing hybrids for organic production systems

Evaluation of Right-of-Way Fence Tags to Reduce Animal-Vehicle Collisions

Animal-vehicle collisions (AVC) pose a serious and growing threat to motorists traveling on ADOT roads. Solutions exist to effectively mitigate AVC, such as wildlife crossing structures (overpasses and underpasses), however these solutions can be costly and untimely. Fence tags, a small reflective tag that is attached to the right-of-way fence and deter animals from crossing the fence, provide a potential cost-effective solution to reduce AVC. The research team evaluated the effectiveness of fence tags designed by Swift Creek, LLC, in reducing AVC. Fence tags were installed along both sides of five 2-mile segments of roadway and maintained for three years by replacing missing or damaged fence tags on a quarterly basis. The research team queried ADOT Crash Data to determine changes in AVC prior to the study (July 1, 2015 \u2013 June 30, 2018) and following fence tag installation (July 1, 2018 \u2013 June 30, 2021). Collisions in the areas where fence tags were installed were reduced by an average of 25.4 percent across all sites. This reduction was significant; however, the control sites were also reduced by a similar average of 25.8 percent across all study sites. These findings indicate that (1) the effectiveness of fence tags at reducing AVC were not noticeably different than the control, or (2) external factors that were not considered in the study, such as the effect of nearby road construction, may have influenced the effectiveness of the fence tags. To better understand the results of this study and improve available data for future projects and management decisions, the research team recommends: (1) further examining fence tags as a viable mitigation technique by looking at other aspects that could be taken into account in the study, such as other external factors affecting their use; measuring the effective tag size; implementing a longer observation period before, during, and after the intervention, etc. and (2) implementing data collection forms that capture animal type/species