5,179 research outputs found
Analytical computation of the off-axis Effective Area of grazing incidence X-ray mirrors
Focusing mirrors for X-ray telescopes in grazing incidence, introduced in the
70s, are characterized in terms of their performance by their imaging quality
and effective area, which in turn determines their sensitivity. Even though the
on-axis effective area is assumed in general to characterize the collecting
power of an X-ray optic, the telescope capability of imaging extended X-ray
sources is also determined by the variation in its effective area with the
off-axis angle. [...] The complex task of designing optics for future X-ray
telescopes entails detailed computations of both imaging quality and effective
area on- and off-axis. Because of their apparent complexity, both aspects have
been, so far, treated by using ray-tracing routines aimed at simulating the
interaction of X-ray photons with the reflecting surfaces of a given focusing
system. Although this approach has been widely exploited and proven to be
effective, it would also be attractive to regard the same problem from an
analytical viewpoint, to assess an optical design of an X-ray optical module
with a simpler calculation than a ray-tracing routine. [...] We have developed
useful analytical formulae for the off-axis effective area of a
double-reflection mirror in the double cone approximation, requiring only an
integration and the standard routines to calculate the X-ray coating
reflectivity for a given incidence angle. [...] Algebraic expressions are
provided for the mirror geometric area, as a function of the off-axis angle.
Finally, the results of the analytical computations presented here are
validated by comparison with the corresponding predictions of a ray-tracing
code.Comment: 12 pages, 11 figures, accepted for publication in "Astronomy &
Astrophysics", section "Instruments, observational techniques, and data
processing". Updated version after grammatical revision and typos correctio
Characterization of multilayer stack parameters from X-ray reflectivity data using the PPM program: measurements and comparison with TEM results
Future hard (10 -100 keV) X-ray telescopes (SIMBOL-X, Con-X, HEXIT-SAT, XEUS)
will implement focusing optics with multilayer coatings: in view of the
production of these optics we are exploring several deposition techniques for
the reflective coatings. In order to evaluate the achievable optical
performance X-Ray Reflectivity (XRR) measurements are performed, which are
powerful tools for the in-depth characterization of multilayer properties
(roughness, thickness and density distribution). An exact extraction of the
stack parameters is however difficult because the XRR scans depend on them in a
complex way. The PPM code, developed at ERSF in the past years, is able to
derive the layer-by-layer properties of multilayer structures from
semi-automatic XRR scan fittings by means of a global minimization procedure in
the parameters space. In this work we will present the PPM modeling of some
multilayer stacks (Pt/C and Ni/C) deposited by simple e-beam evaporation.
Moreover, in order to verify the predictions of PPM, the obtained results are
compared with TEM profiles taken on the same set of samples. As we will show,
PPM results are in good agreement with the TEM findings. In addition, we show
that the accurate fitting returns a physically correct evaluation of the
variation of layers thickness through the stack, whereas the thickness trend
derived from TEM profiles can be altered by the superposition of roughness
profiles in the sample image
The surface accessibility of α-bungarotoxin monitored by a novel paramagnetic probe
The surface accessibility of {alpha}-bungarotoxin has been investigated by using Gd2L7, a newly designed paramagnetic NMR probe. Signal attenuations induced by Gd2L7 on {alpha}-bungarotoxin C{alpha}H peaks of 1H-13C HSQC spectra have been analyzed and compared with the ones previously obtained in the presence of GdDTPA-BMA. In spite of the different molecular size and shape, for the two probes a common pathway of approach to the {alpha}-bungarotoxin surface can be observed with an equally enhanced access of both GdDTPA-BMA and Gd2L7 towards the protein surface side where the binding site is located. Molecular dynamics simulations suggest that protein backbone flexibility and surface hydration contribute to the observed preferential approach of both gadolinium complexes specifically to the part of the {alpha}-bungarotoxin surface which is involved in the interaction with its physiological target, the nicotinic acetylcholine receptor
Association between serum Mg2+ concentrations and cardiovascular organ damage in a cohort of adult subjects
Magnesium (Mg2+) levels are associated with insulin resistance, hypertension, atherosclerosis, and type 2 diabetes (T2DM). We evaluated the clinical utility of physiological Mg2+ in assessing subclinical cardiovascular organ damage including increased carotid artery intima-media thickness (c-IMT) and left ventricular mass index (LVMI) in a cohort of well-characterized adult non-diabetic individuals. Age-and gender-adjusted correlations between Mg2+ and metabolic parameters showed that Mg2+ circulating levels were correlated negatively with body mass index (BMI), fasting glucose, and 2h-oral glucose tolerance test (OGTT) glucose. Similarly, Mg2+ levels were significantly and negatively related to c-IMT and LVMI. A multivariate regression analysis revealed that age (β = 0.440; p < 0.0001), BMI (β = 0.225; p < 0.0001), and Mg2+ concentration (β = −0.122; p < 0.01) were independently associated with c-IMT. Age (β = 0.244; p = 0.012), Mg2+ (β = −0.177; p = 0.019), and diastolic blood pressure (β = 0.184; p = 0.038) were significantly associated with LVMI in women, while age (β = 0.211; p = 0.019), Mg2+ (β = −0.171; p = 0.038) and the homeostasis model assessment index of insulin resistance (HOMA-IR) (β = −0.211; p = 0.041) were the sole variables associated with LVMI in men. In conclusion, our data support the hypothesis that the assessment of Mg2+ as part of the initial work-up might help unravel the presence of subclinical organ damage in subjects at increased risk of cardiovascular complications
Structural investigation of Rett-inducing MeCP2 mutations
X-ray structure of methyl-CpG binding domain (MBD) of MeCP2, an intrinsically disordered protein (IDP) involved in Rett syndrome, offers a rational basis for defining the spatial distribution for most of the sites where mutations responsible of Rett syndrome, RTT, occur. We have ascribed pathogenicity for mutations of amino acids bearing positively charged side chains, all located at the protein-DNA interface, as positive charge removal cause reduction of the MeCP2-DNA adduct lifetime. Pathogenicity of the frequent proline replacements, outside the DNA contact moiety of MBD, can be attributed to the role of this amino acid for maintaining both unfolded states for unbound MeCP2 and, at the same time, to favor some higher conformational order for stabilizing structural determinants required by protein activity. These hypotheses can be extended to transcription repressor domain, TRD, the other MeCP2-DNA interaction site and, in general, to all the IDP that interact with nucleic acids
Simbol-X Hard X-ray Focusing Mirrors: Results Obtained During the Phase A Study
Simbol-X will push grazing incidence imaging up to 80 keV, providing a strong
improvement both in sensitivity and angular resolution compared to all
instruments that have operated so far above 10 keV. The superb hard X-ray
imaging capability will be guaranteed by a mirror module of 100 electroformed
Nickel shells with a multilayer reflecting coating. Here we will describe the
technogical development and solutions adopted for the fabrication of the mirror
module, that must guarantee an Half Energy Width (HEW) better than 20 arcsec
from 0.5 up to 30 keV and a goal of 40 arcsec at 60 keV. During the phase A,
terminated at the end of 2008, we have developed three engineering models with
two, two and three shells, respectively. The most critical aspects in the
development of the Simbol-X mirrors are i) the production of the 100 mandrels
with very good surface quality within the timeline of the mission; ii) the
replication of shells that must be very thin (a factor of 2 thinner than those
of XMM-Newton) and still have very good image quality up to 80 keV; iii) the
development of an integration process that allows us to integrate these very
thin mirrors maintaining their intrinsic good image quality. The Phase A study
has shown that we can fabricate the mandrels with the needed quality and that
we have developed a valid integration process. The shells that we have produced
so far have a quite good image quality, e.g. HEW <~30 arcsec at 30 keV, and
effective area. However, we still need to make some improvements to reach the
requirements. We will briefly present these results and discuss the possible
improvements that we will investigate during phase B.Comment: 6 pages, 3 figures, invited talk at the conference "2nd International
Simbol-X Symposium", Paris, 2-5 december, 200
Mechanistic basis for the activation of plant membrane receptor kinases by SERK-family coreceptors.
Plant-unique membrane receptor kinases with leucine-rich repeat ectodomains (LRR-RKs) can sense small molecule, peptide, and protein ligands. Many LRR-RKs require SERK-family coreceptor kinases for high-affinity ligand binding and receptor activation. How one coreceptor can contribute to the specific binding of distinct ligands and activation of different LRR-RKs is poorly understood. Here we quantitatively analyze the contribution of SERK3 to ligand binding and activation of the brassinosteroid receptor BRI1 and the peptide hormone receptor HAESA. We show that while the isolated receptors sense their respective ligands with drastically different binding affinities, the SERK3 ectodomain binds the ligand-associated receptors with very similar binding kinetics. We identify residues in the SERK3 N-terminal capping domain, which allow for selective steroid and peptide hormone recognition. In contrast, residues in the SERK3 LRR core form a second, constitutive receptor-coreceptor interface. Genetic analyses of protein chimera between BRI1 and SERK3 define that signaling-competent complexes are formed by receptor-coreceptor heteromerization in planta. A functional BRI1-HAESA chimera suggests that the receptor activation mechanism is conserved among different LRR-RKs, and that their signaling specificity is encoded in the kinase domain of the receptor. Our work pinpoints the relative contributions of receptor, ligand, and coreceptor to the formation and activation of SERK-dependent LRR-RK signaling complexes regulating plant growth and development
Nose-pharyngeal swabs as a screening test for SARS-CoV-2 infection in patients with scheduled elective surgery. The experience of the Hygiene Service of Local Health Authority Roma 1
Proactive case investigation and contact tracing, testing, home isolation, in parallel with systematic home care for patients with mild symptoms have served as cornerstones of a territorial response strategy, where an established network of public health and primary care services existed. The Italian National Health Service (NHS) entrusts many management competences to the Regions, with the result of a different management of the Covid-19 epidemic between the Regions and sometimes even among the Local Health Authorities (LHAs - in Italian: Aziende Sanitarie Locali). In Italy, the Department of Prevention (DP) plays a pivotal role in structuring the collaboration between territorial health services, hospitals and all the network of the NHS. DP is the technical structure of LHA, with the task of promoting health, preventing the onset of disease and improving the quality of life of the population. The Covid-19 pandemic has had a considerable impact on the DPs, which have necessarily had to reorganize their services in a very short time in order to provide an appropriate response to the renewed health concerns
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