Costs of State Vehicle and Driver Licensing in Indiana: A Comparison with Other States

Spencer and Main\u27s contribution to Indiana Academy of the Social Sciences Proceedings, 1985

Genome sequence of Acetomicrobium hydrogeniformans OS1

Acetomicrobium hydrogeniformans, an obligate anaerobe of the phylum Synergistetes, was isolated from oil production water. It has the unusual ability to produce almost 4 molecules H2/molecule glucose. The draft genome of A. hydrogeniformans OS1 (DSM 22491T) is 2,123,925 bp, with 2,068 coding sequences and 60 RNA genes

A Fluorogenic Red Fluorescent Protein Heterodimer

SummaryThe expanding repertoire of genetically encoded biosensors constructed from variants of Aequorea victoria green fluorescent protein (GFP) enable the imaging of a variety of intracellular biochemical processes. To facilitate the imaging of multiple biosensors in a single cell, we undertook the development of a dimerization-dependent red fluorescent protein (ddRFP) that provides an alternative strategy for biosensor construction. An extensive process of rational engineering and directed protein evolution led to the discovery of a ddRFP with a Kd of 33 μM and a 10-fold increase in fluorescence upon heterodimer formation. We demonstrate that the dimerization-dependent fluorescence of ddRFP can be used for detection of a protein-protein interaction in vitro, imaging of the reversible Ca2+-dependent association of calmodulin and M13 in live cells, and imaging of caspase-3 activity during apoptosis

An Analysis of Spatio-Temporal Landscape Patterns for Protected Areas in Northern New England: 1099-2010

Context: Landscape ecology theory provides insight about how large assemblages of protected areas (PAs) should be configured to protect biodiversity. We adapted these theories to evaluate whether the emergence of decentralized land protection in a largely private landscape followed the principles of reserve design. Objectives: Our objectives were to determine: (1) Are there distinct clusters of PAs in time and space? (2) Are PAs becoming more spatially clustered through time? and (3) Does the resulting PA portfolio have traits characteristic of ideal reserve design? Methods: We developed an historical dataset of the PAs enacted since 1900 in the northern New England region of the US. We conducted spatio-temporal clustering, landscape pattern, and aggregation analyses at both the landscape scale and for specific classes of land ownership, conservation method, and degree of protection. Results: We found the frequency of PAs increased through time, and that area-weighted clusters of PAs were heavily influenced by a few recent large PAs. PA clustering around preexisting PAs was driven primarily by establishment of large PAs focused on natural resource management, rather than strict reserves. Since 1990, the complete portfolio has increased in aggregation, but reserve patches have become less aggregated and smaller, while patches that allow extractive uses have become more aggregated and larger. Conclusions: Our extension of landscape ecology theory to a diverse portfolio of PAs underscores the importance of prioritizing conservation choices in the context of existing PAs, and elucidates the landscape scale effects of individual actions within a portfolio of protected areas

An Analysis of Spatio-Temporal Landscape Patterns for Protected Areas in Northern New England: 1099-2010

Context: Landscape ecology theory provides insight about how large assemblages of protected areas (PAs) should be configured to protect biodiversity. We adapted these theories to evaluate whether the emergence of decentralized land protection in a largely private landscape followed the principles of reserve design. Objectives: Our objectives were to determine: (1) Are there distinct clusters of PAs in time and space? (2) Are PAs becoming more spatially clustered through time? and (3) Does the resulting PA portfolio have traits characteristic of ideal reserve design? Methods: We developed an historical dataset of the PAs enacted since 1900 in the northern New England region of the US. We conducted spatio-temporal clustering, landscape pattern, and aggregation analyses at both the landscape scale and for specific classes of land ownership, conservation method, and degree of protection. Results: We found the frequency of PAs increased through time, and that area-weighted clusters of PAs were heavily influenced by a few recent large PAs. PA clustering around preexisting PAs was driven primarily by establishment of large PAs focused on natural resource management, rather than strict reserves. Since 1990, the complete portfolio has increased in aggregation, but reserve patches have become less aggregated and smaller, while patches that allow extractive uses have become more aggregated and larger. Conclusions: Our extension of landscape ecology theory to a diverse portfolio of PAs underscores the importance of prioritizing conservation choices in the context of existing PAs, and elucidates the landscape scale effects of individual actions within a portfolio of protected areas

Exploring Aerosols near Clouds with High-Spatial-Resolution Aircraft Remote Sensing During SEAC4RS

Since aerosols are important to our climate system, we seek to observe the variability of aerosol properties within cloud systems. When applied to the satelliteborne Moderateresolution Imaging Spectroradiometer (MODIS), the Dark Target retrieval algorithm provides global aerosol optical depth (AOD; at 0.55 m) in cloudfree scenes. Since MODIS' resolution (500m pixels, 3 or 10km product) is too coarse for studying nearcloud aerosol, we ported the Dark Target algorithm to the highresolution (~50m pixels) enhancedMODIS Airborne Simulator (eMAS), which flew on the highaltitude ER2 during the Studies of Emissions, Atmospheric Composition, Clouds, and Climate Coupling by Regional Surveys Airborne Science Campaign over the United States in 2013. We find that even with aggressive cloud screening, the ~0.5km eMAS retrievals show enhanced AOD, especially within 6 km of a detected cloud. To determine the cause of the enhanced AOD, we analyze additional eMAS products (cloud retrievals and degradedresolution AOD), coregistered Cloud Physics Lidar profiles, MODIS aerosol retrievals, and groundbased Aerosol Robotic Network observations. We also define spatial metrics to indicate local cloud distributions near each retrieval and then separate into nearcloud and farfromcloud environments. The comparisons show that low cloud masking is robust, and unscreened thin cirrus would have only a small impact on retrieved AOD. Some of the enhancement is consistent with clearcloud transition zone microphysics such as aerosol swelling. However, 3D radiation interaction between clouds and the surrounding clear air appears to be the primary cause of the high AOD near clouds

Land Conservation in the Northeastern United States: An Assessment of Historic Trends and Current Conditions

This article discusses the evolution of land conservation efforts and outcomes in the Northeast, examine major drivers of landscape change, and review key conservation tools that have been used to protect public values at the local and landscape levels. We then assess the current status of land conservation, and draw lessons for other regions facing conservation challenges across mixed ownership landscapes under varying development and land-use pressures. Finally, we explore new and emerging trends in the factors driving land development and conservation activities in an effort to assess the challenges that lie ahead

Design of the ARES Mars Airplane and Mission Architecture

Significant technology advances have enabled planetary aircraft to be considered as viable science platforms. Such systems fill a unique planetary science measurement gap, that of regional-scale, near-surface observation, while providing a fresh perspective for potential discovery. Recent efforts have produced mature mission and flight system concepts, ready for flight project implementation. This paper summarizes the development of a Mars airplane mission architecture that balances science, implementation risk and cost. Airplane mission performance, flight system design and technology maturation are described. The design, analysis and testing completed demonstrates the readiness of this science platform for use in a Mars flight project

Glacier meltwater and monsoon precipitation drive Upper Ganges Basin dissolved organic matter composition

Author Posting. © The Author(s), 2019. This is the author's version of the work. It is posted here by permission of Elsevier Ltd. for personal use, not for redistribution. The definitive version was published in Geochimica et Cosmochimica Acta 244 (2019): 216-228, doi:10.1016/j.gca.2018.10.012.Mountain glaciers store dissolved organic carbon (DOC) that can be exported to river networks and subsequently respired to CO2. Despite this potential importance within the global carbon cycle, the seasonal variability and downstream transport of glacier-derived DOC in mountainous river basins remains largely unknown. To provide novel insight, here we present DOC concentrations and molecular-level dissolved organic matter (DOM) compositions from 22 nested, glaciated catchments (1.4 – 81.8 % glacier cover by area) in the Upper Ganges Basin, Western Himalaya over the course of the Indian summer monsoon (ISM) in 2014. Aliphatic and peptide-like compounds were abundant in glaciated headwaters but were overprinted by soil-derived phenolic, polyphenolic and condensed aromatic material as DOC concentrations increase moving downstream. Across the basin, DOC concentrations and soil-derived compound class contributions decreased sharply from pre- to post-ISM, implying increased relative contribution of glaciated headwater signals as the monsoon progresses. Incubation experiments further revealed a strong compositional control on the fraction of bioavailable DOC (BDOC), with glacier-derived DOC exhibiting the highest bioavailability. We hypothesize that short-term (i.e. in the coming decades) increases in glacier melt flux driven by climate change will further bias exported DOM toward an aliphatic-rich, bioavailable signal, especially during the ISM and post-ISM seasons. In contrast, eventual decreases in glacier melt flux due to mass loss will likely lead to more a soil-like DOM composition and lower bioavailability of exported DOC in the long term.We thank Britta Voss (WHOI) for assisting with sample collection; Travis Drake (FSU), and Ekaterina Bulygina (Woods Hole Research Center) for laboratory assistance; and the NHMFL ICR user program (NSF-DMR-1157490) for aiding in data acquisition and analysis. This study was partly supported by NSF-DEB-1145932 to R.G.M.S. J.D.H. was partially supported by the NSF Graduate Research Fellowship Program under grant number 2012126152, with additional support in the form of travel grants awarded by the MIT Houghten Fund and NHMFL. All data used in this study are available in the Supporting Information Tables S1 and S2