Accelerometers can measure total and activity-specific energy expenditures in free-ranging marine mammals only if linked to time-activity budgets

Peer reviewedPostprin

Physical activity energy expenditure has not declined since the 1980s and matches energy expenditures of wild mammals.

Objective:Obesity results from protracted energy imbalance. Whether this comprises excessive energy intake, lowered physical activity or both, remains disputed.Design:Physical activity energy expenditure, evaluated in three different ways from daily energy expenditure (DEE) measured using doubly labelled water, was examined for trends over time. Data included subjects in Europe (Maastricht, the Netherlands) and North America extending back to the 1980s. These data were compared with measures from the third world, and measures made on wild terrestrial mammals.Results:Physical activity expenditure in Europe (residual of the regression of DEE on basal energy expenditure (BEE)) has slightly but significantly increased since the 1980s. There was no trend over time in physical activity level (PAL=DEE/BEE), or in the residual variance in DEE once mass, sex and age were accounted for. This latter index of physical activity expenditure also significantly increased over time in North America. DEE of individuals in Europe and North America was not significantly different from individuals measured in the third world. In wild terrestrial mammals, DEE mostly depended on body mass and ambient temperature. Predicted DEE for a 78 kg mammal living at 20 degrees C was 9.2 MJ per day (95% CI: 7.9-12.9 MJ per day), not significantly different from the measured DEE of modern humans (around 10.2-12.6 MJ per day).Conclusion:As physical activity expenditure has not declined over the same period that obesity rates have increased dramatically, and daily energy expenditure of modern man is in line with energy expenditure in wild mammals, it is unlikely that decreased expenditure has fuelled the obesity epidemic.International Journal of Obesity advance online publication, 27 May 2008; doi:10.1038/ijo.2008.74

Thermoregulatory and cardiovascular responses to creatine, glycerol and alpha lipoic acid in trained cyclists

<p>Abstract</p> <p>Background</p> <p>It has been shown that supplementation with creatine (Cr) and glycerol (Gly), when combined with glucose (Glu) necessary for the enhancement of Cr uptake by skeletal muscle, induces significant improvements in thermoregulatory and cardiovascular responses during exercise in the heat.</p> <p>Purpose</p> <p>To determine whether Cr/Gly-induced thermoregulatory and cardiovascular responses are maintained when the majority (~75%) of the Glu in the Cr/Gly supplement is replaced with the insulintropic agent alpha lipoic acid (Ala).</p> <p>Methods</p> <p>22 healthy endurance trained cyclists were randomly assigned to receive either 20 g/day (4 × 5 g/day) of Cr, 2 g ^.kg^-1 BM per day (4 × 0.5 g ^.kg^-1 BM per day) of Gly and 150 g/day (4 × 37.5 g/day) of Glu or 20 g/day (4 × 5 g/day) of Cr monohydrate, 2 g ^.kg^-1 BM per day (4 × 0.5 g ^.kg^-1 BM per day) of Gly (100 g/day (4 × 25 g/day) of Glu and 1000 mg/day (4 × 250 mg/day) of Ala for 7 days for 7 days. Exercise trials were conducted pre- and post-supplementation and involved 40 min of constant-load cycling exercise at 70% O₂ max by a self-paced 16.1 km time trial at 30°C and 70% relative humidity.</p> <p>Results</p> <p>Median and range values of TBW increased significantly by 2.1 (1.3-3.3) L and 1.8 (0.2-4.6) L in Cr/Gly/Glu and Cr/Gly/Glu/Ala groups respectively (<it>P</it> = 0.03) and of BM not significantly by 1.8 (0.2-3.0) kg and 1.2 (0.5-2.1) kg in Cr/Gly/Glu and in Cr/Gly/Glu/Ala, respectively (<it>P</it> = 0.75). During constant load exercise, heart rate (HR) and core temperature (Tcore) were significantly lower post-supplementation: HR was reduced on average by 3.3 ± 2.1 beats/min and by 4.8 ± 3.3 beats/min (mean ± SD) and Tcore by 0.2 ± 0.1 (mean ± SD) in the Cr/Gly/Glu and Cr/Gly/Glu/Ala, respectively The reduction in HR and Tcore was not significantly different between the supplementation groups.</p> <p>Conclusions</p> <p>In comparison to the established hyper hydrating Cr/Gly/Glu supplement, supplement containing Cr/Gly/Ala and decreased amount of Glu provides equal improvements in thermoregulatory and cardiovascular responses during exercise in the heat.</p

Effects of macronutrient composition on specific dynamic action in the laboratory mouse, Mus musculus

Open-flow respirometry was used to measure the magnitude of specific dynamic action (SDA), the maximum rate of oxygen consumption and the length of time that the rate of oxygen uptake remained elevated above pre-feeding level in MF1 female mice fed different macronutrient diets. Irrespective of diet, the metabolic rate increased immediately after feeding and reached a maximum within 1.7 hours. The composition of the diet significantly altered the magnitude of SDA. SDA was highest for high protein-fed mice (9.4%), followed by high carbohydrate-fed mice (6.1%) and lastly high and medium fat-fed mice (3.9% and 4.5%).KEY WORDS: Specific dynamic action, resting metabolic rate, macronutrient diets, open-flow respirometry, respiratory quotient, energy expenditur

Effects of dietary protein intake on lactation performance of the laboratory mouse, Mus musculus

Laboratory mice (strain MF1) were used to investigate the effects of dietary protein content on lactation performance to test the heat dissipation limit hypothesis. The specific dynamic action (SDA) for high protein (HP) and high carbohydrate (HC) diets was measured using open-flow respirometry at 9.4% and 6.1%, respectively. The same two diets were fed ad libitum to mice during lactation. Mice fed on HP and HC diets at 21 ºC reached a plateau in their daily food intake at 12.3±0.2 g day-1 and 16.6±0.2 g day-1, respectively between days 12-17 of lactation. HP-fed mice had a significantly higher daily energy expenditure (DEE) measured by doubly labelled water and higher water turnover than HC-fed mice but the energy they exported as milk was significantly lower than that of HC-fed mice and therefore resulted in poor growth rate of their offspring. The urea production of HP-fed mice from their daily protein intake of 7.1 g was estimated at 1994 mg which required 10.2 mls of water per day to be cleared. The mice increased their urine production by 14.4 mls probably to eliminate this urea. High protein diet had negative effects on lactation, indicating the growth of pups in previous studies was not protein limited. The negative effects of the HP diet were due to the high DEE that greatly reduced the energy available for milk production, rather than a toxicity effect of the urea production. The different DEE of the two diets suggests that other factors were involved in the delivery of energy to the offspring.KEY WORDS: Laboratory mouse, dietary protein, specific dynamic action, and daily energy expenditur

Effects of feeding fat on lactation performance of the laboratory mouse, Mus musculus

Laboratory mice (strain MF1) were used to investigate the effects of dietary fat intake on lactation performance. The specific dynamic action (SDA) for high fat (HF), medium fat (MF) and a low fat (LF) diets was measured using open-flow respirometry at 4.5%, 3.9% and 6.1%, respectively. The same three diets were fed ad libitum to mice between days 4 and 18 of lactation. Mice fed HF, MF and LF diets reached plateau in their daily food intake at 14.95±1.14 g day-1, 16.30±0.61 g day-1 and 16.57±0.26 g day-1, respectively between days 12-17 of lactation. At weaning, litters from HF and MF-fed mice were significantly heavier than pups on LF diet. This was because the HF and MF-fed mice not only consumed more energy at peak lactation but also delivered more milk energy to their pups than the LF-fed mice. Evidence suggested that the positive effects of feeding fat to mice were in part due to the low SDA and probably low heat production for milk synthesis. Probably, the ability of the HF and MF-fed mice to directly transfer absorbed fat into the milk might have reduced the heat production of lactogenesis. The HF and MF diets had beneficial effects on lactation because they increased the capacity of mice to generate milk more efficiently and wean heavier offspring than mice fed LF diet. The daily energy expenditure (DEE) of mice in the three dietary groups was fixed.KEY WORDS: Laboratory mouse, dietary fat, specific dynamic action, doubly labelled water, daily energy expenditure, milk energy output, reproductive performanc

Inter- and intra-beach thermal variation for Green Turtle nests on Ascension Island, South Atlantic

Nest temperatures for green turtles (Chelonia mydas) nesting on Ascension Island, South Atlantic (7&deg;57\u27S 14&deg;22\u27W), were examined. Temperature probes were placed into nests on two beaches, Long Beach (26 nests) and North East Bay (8 nests). Within these beaches there was relatively little thermal variation (SD of nest temperature was 0.32&deg;C for Long Beach and 0.30&deg;C for North East Bay). To examine inter-beach thermal variation temperature probes were buried at 55 cm on 12 beaches. Inter-beach thermal variation was large and was related to the beach albedo with the darkest beach (albedo, 016) being 4.2&deg;C warmer than the lightest coloured beach (albedo, 0.73)

Inter- and intra-beach thermal variation for Green Turtle nests on Ascension Island, South Atlantic

Nest temperatures for green turtles (Chelonia mydas) nesting on Ascension Island, South Atlantic (7&deg;57\u27S 14&deg;22\u27W), were examined. Temperature probes were placed into nests on two beaches, Long Beach (26 nests) and North East Bay (8 nests). Within these beaches there was relatively little thermal variation (SD of nest temperature was 0.32&deg;C for Long Beach and 0.30&deg;C for North East Bay). To examine inter-beach thermal variation temperature probes were buried at 55 cm on 12 beaches. Inter-beach thermal variation was large and was related to the beach albedo with the darkest beach (albedo, 016) being 4.2&deg;C warmer than the lightest coloured beach (albedo, 0.73)

The role of insulin receptor substrate 2 in hypothalamic and β cell function

Insulin receptor substrate 2 (Irs2) plays complex roles in energy homeostasis. We generated mice lacking Irs2 in β cells and a population of hypothalamic neurons (RIPCreIrs2KO), in all neurons (NesCreIrs2KO), and in proopiomelanocortin neurons (POMCCreIrs2KO) to determine the role of Irs2 in the CNS and β cell. RIPCreIrs2KO mice displayed impaired glucose tolerance and reduced β cell mass. Overt diabetes did not ensue, because β cells escaping Cre-mediated recombination progressively populated islets. RIPCreIrs2KO and NesCreIrs2KO mice displayed hyperphagia, obesity, and increased body length, which suggests altered melanocortin action. POMCCreIrs2KO mice did not display this phenotype. RIPCreIrs2KO and NesCreIrs2KO mice retained leptin sensitivity, which suggests that CNS Irs2 pathways are not required for leptin action. NesCreIrs2KO and POMCCreIrs2KO mice did not display reduced β cell mass, but NesCreIrs2KO mice displayed mild abnormalities of glucose homeostasis. RIPCre neurons did not express POMC or neuropeptide Y. Insulin and a melanocortin agonist depolarized RIPCre neurons, whereas leptin was ineffective. Insulin hyperpolarized and leptin depolarized POMC neurons. Our findings demonstrate a critical role for IRS2 in β cell and hypothalamic function and provide insights into the role of RIPCre neurons, a distinct hypothalamic neuronal population, in growth and energy homeostasis

Thermal conductivity of sand and its effect on the temperature of Loggerhead Sea Turtle (Caretta Caretta) nests

The conductivity of sand at a depth of 30&ndash;50 cm was measured at 15 sites on the beach at Captiva Island in south-west Florida which is used by nesting loggerhead turtles (Caretta caretta). The mean daily temperature of the sand was correlated with conductivity at the same depth measured the same day (r=0&middot;611). When day to day variation was removed the correlation between nest temperature and conductivity increased to 0&middot;694. The sand was highly variable in its grain structure. The dominant variability (80&middot;6%) was redescribed by the first two principal components of a Principal Components Analysis (PCA). These two components were influenced mostly by percentages of large (&gt; 1 mm) and small (&lt; 500 &mu;m) grains respectively. Conductivity was strongly correlated with the grain structure of the sand. The first three principal components describing sand grain structure, explained 84&middot;1% of the variation in conductivity. Moisture content of the sand (always &lt; 5%) was not an important factor. Sites dominated by larger grains generally had poorer conductivity and were cooler. Comparisons of eight nests to seven adjacent random sites revealed no strong evidence for directional selection in nest placement relative to sand conductivity. The variance in conductivities recorded at nests was also not significantly different from the variance at random sites