Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates

Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted fluorescence microscope frame using the method of oblique plane microscopy (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging of 3-D biological cultures in commercially available glass-bottomed 96-well plates using a stage-scanning OPM approach (ssOPM). Time-lapse 3-D imaging of multicellular spheroids expressing a glucose Förster resonance energy transfer (FRET) biosensor is demonstrated in 16 fields of view with image acquisition at 10 minute intervals. As a proof-of-principle, the ssOPM system is also used to acquire a dose response curve with the concentration of glucose in the culture medium being varied across 42 wells of a 96-well plate with the whole acquisition taking 9 min. The 3-D image data enable the FRET ratio to be measured as a function of distance from the surface of the spheroid. Overall, the results demonstrate the capability of the OPM system to measure spatio-temporal changes in FRET ratio in 3-D in multicellular spheroids over time in a multi-well plate format

The automated array assembly task of the low-cost silicon solar array project, phase 2

Several specific processing steps as part of a total process sequence for manufacturing silicon solar cells were studied. Ion implantation was identified as the preferred process step for impurity doping. Unanalyzed beam ion implantation was shown to have major cost advantages over analyzed beam implantation. Further, high quality cells were fabricated using a high current unanalyzed beam. Mechanically masked plasma patterning of silicon nitride was shown to be capable of forming fine lines on silicon surfaces with spacings between mask and substrate as great as 250 micrometers. Extensive work was performed on advances in plated metallization. The need for the thick electroless palladium layer was eliminated. Further, copper was successfully utilized as a conductor layer utilizing nickel as a barrier to copper diffusion into the silicon. Plasma etching of silicon for texturing and saw damage removal was shown technically feasible but not cost effective compared to wet chemical etching techniques

Electronic structure of NiS1−x_{1-x}Sex_x across the phase transition

We report very highly resolved photoemission spectra of NiS(1-x)Se(x) across the so-called metal-insulator transition as a function of temperature as well as composition. The present results convincingly demonstrate that the low temperature, antiferromagnetic phase is metallic, with a reduced density of states at E$_F$. This decrease is possibly due to the opening of gaps along specific directions in the Brillouin zone caused by the antiferromagnetic ordering.Comment: Revtex, 4 pages, 3 postscript figure

VOLCANIC HAZARD ASSESSMENT AT SANTORINI VOLCANO: A REVIEW AND A SYNTHESIS IN THE LIGHT OF THE 2011-2012 SANTORINI UNREST

Το 2011 και το πρώτο εξάμηνο του 2012 η Σαντορίνη γνώρισε την πρώτη σεισμο-ηφαιστειακή κρίση από το 1950, όπως αυτή ανιχνεύθηκε από τα μόνιμα εγκατεστημένα δίκτυα παρακολούθησης και ένα μεγάλο αριθμό παροδικών μετρήσεων. Η διέγερση αυτή χαρακτηρίστηκε από μικρού μεγέθους, αλλά έντονη σεισμική δραστηριότητα, σημαντική ανύψωση και διόγκωση του νησιού, αλλαγές της θερμοκρασίας του νερού και των γεωχημικών αερίων. Ενώ η διέγερση έληξε την άνοιξη του 2012, το παγκόσμιο ενδιαφέρον οδήγησε στην εκπόνηση αρκετών μελετών, σε μια προσπάθεια να αξιολογηθούν τα πιθανά σενάρια για την εξέλιξη της διέγερσης. Στο πλαίσιο αυτό, συνοψίζουμε τα σημαντικότερα ευρήματα σχετικά με την επικινδυνότητα του ευρύτερου ηφαιστειακού συγκροτήματος της Σαντορίνης, καθώς και τις πληροφορίες που πρέπει να ληφθούν υπόψη για τη διαχείριση μιας πιθανής μελλοντικής ηφαιστειακής κρίσης.In 2011 and the first half of 2012 Santorini experienced its first seismo-volcanic unrest since 1950, as detected by the permanently installed monitoring networks and a large number of campaign measurements. The unrest was characterized by small magnitude but intense seismic activity, significant uplift and inflation deformation rates, and changes of water temperature as well as of fluid and soil gases. While the unrest ended in the spring of 2012, the world-wide interest led to the performance of several studies, in an attempt to assess the possible scenarios for the unrest evolution. Within this framework, we summarize the most important findings regarding the volcanic hazard assessment of the broader Santorini volcanic complex, as well as the constraints that need to be taken into account for a possible future volcanic crisis management

Comments on F-maximization and R-symmetry in 3D SCFTs

We report preliminary results on the recently proposed F-maximization principle in 3D SCFTs. We compute numerically in the large-N limit the free energy on the three-sphere of an N=2 Chern-Simons-Matter theory with a single adjoint chiral superfield which is known to exhibit a pattern of accidental symmetries associated to chiral superfields that hit the unitarity bound and become free. We observe that the F-maximization principle produces a U(1) R-symmetry consistent with previously obtained bounds but inconsistent with a postulated Seiberg-like duality. Potential modifications of the principle associated to the decoupling fields do not appear to be sufficient to account for the observed violations.Comment: 17 pages, 3 figures; v2 a reference has been added, a missing factor of 2 has been corrected in eq (3.3) and the numerical results have been accordingly updated. The new results do not show any obvious signs of violation of previously obtained bounds. A potential disagreement with a postulated Seiberg-like duality is note

Stability of Bose Einstein condensates of hot magnons in YIG

We investigate the stability of the recently discovered room temperature Bose-Einstein condensate (BEC) of magnons in Ytrrium Iron Garnet (YIG) films. We show that magnon-magnon interactions depend strongly on the external field orientation, and that the BEC in current experiments is actually metastable - it only survives because of finite size effects, and because the BEC density is very low. On the other hand a strong field applied perpendicular to the sample plane leads to a repulsive magnon-magnon interaction; we predict that a high-density magnon BEC can then be formed in this perpendicular field geometry.Comment: Submitted to Physical Review Letter

Cutaneous squamous cell carcinoma staging may influence management in users: A survey study.

PURPOSE: This study aims to determine whether there is consensus regarding staging and management of cutaneous squamous cell carcinoma (CSCC) across the various specialties that manage this disease. MATERIALS AND METHODS: A survey regarding CSCC high-risk features, staging, and management was created and emailed to cutaneous oncology experts including dermatology, head and neck surgery/surgical oncology, radiation oncology, and medical oncology. RESULTS: One hundred fifty-six (46%) of 357 invited physicians completed the survey. Depth of invasion (92%), perineural invasion (99%), histologic differentiation (85%), and patient immunosuppression (90%) achieved consensus (\u3e80%) as high-risk features of CSCC. Dermatologists were more likely to also choose clinical tumor diameter (79% vs. 54%) and histology (99% vs. 66%) as a high-risk feature. Dermatologists were also more likely to utilize the Brigham and Women\u27s Hospital (BWH) staging system alone or in conjunction with American Joint Committee on Cancer (AJCC) (71%), whereas other cancer specialists (OCS) tend to use only AJCC (71%). Respondents considered AJCC T3 and higher (90%) and BWH T2b and higher (100%) to be high risk and when they consider radiologic imaging, sentinel lymph node biopsy, post-operative radiation therapy, and increased follow-up. Notably, a large number of respondents do not use staging systems or tumor stage to determine treatment options beyond surgery in high-risk CSCC. CONCLUSION: This survey study highlights areas of consensus and differences regarding the definition of high-risk features of CSCC, staging approaches, and management patterns between dermatologists and OCS. High-risk CSCC is defined as, but not limited to, BWH T2b and higher and AJCC T3 and higher, and these thresholds can be used to identify cases for which treatment beyond surgery may be considered. Dermatologists are more likely to utilize BWH staging, likely because BWH validation studies showing advantages over AJCC were published in dermatology journals and discussed at dermatology meetings. Additional data are necessary to develop a comprehensive risk-based management approach for CSCC

A Deformation of Sasakian Structure in the Presence of Torsion and Supergravity Solutions

We discuss a deformation of Sasakian structure in the presence of totally skew-symmetric torsion by introducing odd dimensional manifolds whose metric cones are K\"ahler with torsion. It is shown that such a geometry inherits similar properties to those of Sasakian geometry. As an example of them, we present an explicit expression of local metrics and see how Sasakian structure is deformed by the presence of torsion. We also demonstrate that our example of the metrics admits the existence of hidden symmetries described by non-trivial odd-rank generalized closed conformal Killing-Yano tensors. Furthermore, using these metrics as an {\it ansatz}, we construct exact solutions in five dimensional minimal (un-)gauged supergravity and eleven dimensional supergravity. Finally, we discuss the global structures of the solutions and obtain regular metrics on compact manifolds in five dimensions, which give natural generalizations of Sasaki--Einstein manifolds $Y^{p,q}$ and $L^{a,b,c}$. We also discuss regular metrics on non-compact manifolds in eleven dimensions.Comment: 38 pages, 1 table, v2: version to appear in Class. Quant. Gra

Some factors influencing populations of the European corn borer, Ostrinia nubilalis (Hubner) in the north central states: Resistance of corn, time of planting and weather conditions Part II, 1958-1962

A cooperative project was conducted by the agricultural experiment stations of Iowa, Minnesota and Ohio and the U. S. Department of Agriculture to study the effects of weather, planting date and resistant hybrids as factors influencing populations of the European com borer, Ostrinia nubilalis (Hübner). Identical studies were carried out at Ankeny, Iowa; Waseca, Minnesota; and Wooster, Ohio, during a 10-year period, 1953-1962. The first 4 years of the study (1953-56) were reported by Everett et al. (1958). The work reported herein is a companion bulletin to the Everett et al. (1958) publication and deals with the results of experiments conducted during 1958-1962. The experimental design was a randomized block, split plot with five replications. The whole plot treatments were four hybrid-planting date combinations consisting of early- or late-planting dates and susceptible or resistant hybrids. The subplot treatments consisted of a factorial arrangement of all possible combinations of three levels of infestation (zero, natural and natural + 3 egg masses) by first brood and the same three levels of infestation by second-brood borers. Temperature and rainfall records were kept at each of the three stations. Borer population and injury to the plant were recorded at the end of the first brood and in the fall. Yield data were collected