Classes of exact wavefunctions for general time-dependent Dirac Hamiltonians in 1+1 dimensions

In this work we construct two classes of exact solutions for the most general time-dependent Dirac Hamiltonian in 1+1 dimensions. Some problems regarding to some formal solutions in the literature are discussed. Finally the existence of a generalized Lewis-Riesenfeld invariant connected with such solutions is discussed

USE OF COMBINATION OF FLUORESCENT PROBES TO IDENTIFY SPERM SUBPOPULATIONS FOR QUALITY ASSESSMENT OF FRESH AND CRYOPRESERVED CANINE SEMEN. PRELIMINARY RESULTS

The use of fluorescent markers in the evaluation of sperm morphophysiology allows a better accuracy, compared to the subjective nature of some routine tests in semen qualification. In this study was used the combination of fluorescence probes: propionate iodide, Hoechst 33342 and FITC-PSA in fresh and thawed dog semen, to the identification of the following morphological subpopulations: II (intact plasma and acrosomal membranes), IL (intact plasma membrane and lesioned acrosomal membrane), LI (lesioned plasma membrane and intact acrosomal membrane) and LL (both membranes lesioned). When comparing the results obtained with the results of the tests used conventionally in semen evaluation (sperm motility and vigor, hypoosmotic test and morphological alterations), little correlation was observed. The II population declined from fresh semen to thawed, while LL population increased (p <0.05). The IL population was composed of extremely small numbers of cells but increased (p <0.05) from fresh semen to thawed semen. In the thawed semen the major defects had a positive correlation with the LL population (p <0.01). For the thawed semen, the results of the hypoosmotic test (number of cells that reacted to the medium) correlated positively with population II (p <0.025), that is, different from that observed in fresh semen. Although all tests were able to detect decrease in sperm quality post-thawing (p <0.05). The use of this fluorescent probe association allowed qualification and more accurately quantification of plasma membrane and acrosomal insults mediated by cryopreservation. El uso de marcadores fluorescentes en la evaluación de la morfofisiología espermática permite una mayor precisión, comparada con la naturaleza subjetiva de algunas pruebas de rutina en la valoración del semen. En este estudio se usó la combinación de pruebas fluorescentes: yoduro de propidio; Hoechst 33342 y FITC-PSA en semen fresco y descongelado de perro, para la identificación de las siguientes subpoblaciones morfológicas: II (membranas plasmática y acrosomal intactas), IL (membrana plasmática intacta y membrana acrosomal dañada), LI (membrana plasmática dañada y membrana acrosomal intacta) y LL (ambas membranas dañadas). Cuando se comparan los resultados obtenidos con los resultados de las pruebas usadas convencionalmente en la evaluación seminal (motilidad y vigor espermáticos, prueba hipoosmótica y alteraciones morfológicas), se observó poca correlación. La población II disminuyó desde el semen fresco al descongelado, mientras que la población LL se incrementó (p<0.05). la población IL estuvo compuesta por un número extremadamente pequeño de células, pero incremento (p<0.05) desde el semen fresco al descongelado. En el semen descongelado los defectos mayores tuvieron una correlación positiva con la población LL (p<0.01). En el semen descongelado, los resultados de la prueba hipoosmótica (número de células que reaccionan al medio) se correlacionaron positivamente con la población II (p<0.05). El uso de esta asociación de pruebas fluorescentes permitió la valoración y la cuantificación más precisa de los daños a la membrana plasmática y acrosomal mediados por la criopreservación.

Cytotoxic Effects Of Zoledronic Acid On Human Epithelial Cells And Gingival Fibroblasts

Bisphosphonate-induced osteonecrosis has been related to the cytotoxicity of these drugs on oral mucosa cells. A previous study showed that 5 μM of zoledronic acid (ZA), a nitrogen-containing bisphosphonate, is the highest concentration of this drug found in the oral cavity of patients under treatment. Therefore, in order to simulate an osteonecrosis clinical condition, the aim of this study was to evaluate the highest concentration of ZA applied on human epithelial cells (HaCaT) and gingival fibroblasts. For this purpose, cells (3x104 cells/cm2) were seeded in wells for 48 h using complete culture medium (cDMEM). After 48 h incubation, the cDMEM was replaced by fresh serum-free culture medium (DMEM-FBS) in which the cells were maintained for additional 24 h. Then, 5 μM ZA were added to the DMEM-FBS and the cells incubated in contact with the drug for 48 h. After this period, the number of viable cells (trypan blue), cell viability (MTT assay), total protein (TP) production and cell morphology (SEM analysis) were assessed. Data were analyzed statistically by Mann-Whitney, ANOVA and Tukey's test (α=0.05). ZA caused a significant reduction in the number of viable cells and decreased the metabolic activity of both cell lines. However, decrease of TP production occurred only in the epithelial cell cultures. Morphological alterations were observed in both cell types treated with ZA. In conclusion, ZA (5 μM) was cytotoxic to human epithelial cells and gingival fibroblast cultures, which could be associated, clinically, with the development of bisphosphonateinduced osteonecrosis.246551558Civitelli, R., Napoli, N., Armamento-Villareal, R., Use of intravenous bisphosphonates in osteoporosis (2007) Curr Osteoporos Rep, 5, pp. 8-13Cohen, S.B., An update on bisphosphonates (2004) Curr Rheumatol Rep, 6, pp. 59-65Rogers, M.J., Watts, D.J., Russel, R.G., Overview of bisphosphonates (1997) Cancer, 80, pp. 1652-1660Rogers, M.J., Gordon, S., Benford, H.L., Coxon, F.P., Luckman, S.P., Monkkonen, J., Cellular and molecular mechanisms of action of bisphosphonates (2000) Cancer Supl, 88, pp. 2961-2978Lawson, M.A., Xia, Z., Barnett, B.L., Triffitt, J.T., Phipps, R.J., Dunford, J.E., Differences between bisphosphonates in binding affinities for hydroxyapatite (2010) J Biomed Mater Res Part B: Appl Biomater, 92, pp. 149-155Allen, M.R., Burr, D.B., The pathogenesis of bisphosphonate-related osteonecrosis of the jaw: So many hypotheses, so few data (2009) J Oral Maxillofac Surg, 67, pp. 61-70Otto, S., Pautke, C., Opelz, C., Wesphal, I., Drosse, I., Swager, J., Osteonecrosis of the jaw: Effects of bisphosphonate type, local concentration, and acidic milieu on the pathomechanism (2010) J Oral Maxillofac Surg, 68, pp. 2837-2845Reid, I.R., Booland, M.J., Is bisphosphonate-associated osteonecrosis of the jaw caused by soft tissue toxicity? (2007) Bone, 41, pp. 318-320Scheper, M.A., Badros, A., Chausparat, R., Cullen, K.J., Meiller, T.F., Effect of zoledronic acid on oral fibroblasts and epithelial cells: A potential mechanism of bisphosphonate-associated osteonecrosis (2009) Br J Haematol, 144, pp. 667-676Scheper, M.A., Badros, A., Salama, A.R., Wartburton, G., Cullen, K.J., Weikel, D.S., A novel bioassay model to determine clinically significant bisphosphonate levels (2009) Support Care Cancer, 17, pp. 1553-1557Ruggiero, S.L., Mehrotra, B., Rosenberg, T.J., Engroff, S.L., Osteonecrosis of the jaws associated with the use of bisphosphonates: A review of 63 cases (2004) J Oral Maxillofac Surg, 62, pp. 527-534Walter, C., Klein, M.O., Pabst, A., Al-Nawas, B., Duscher, H., Ziebart, T., Influence of bisphosphonates on endothelial cells, fibroblasts, and osteogenic cells (2010) Clin Oral Investig, 14, pp. 35-41Kumar, S.K.S., Gorur, A., Schaauddin, C., Shuler, C.F., Costerton, J.W., Sedghizadeh, P.P., The role of microbial biofilms in osteonecrosis of the jaw associated with bisphosphonate therapy (2010) Curr Osteoporos Rep, 8, pp. 40-48Aas, J.A., Paster, B.J., Stokes, L.N., Olsen, I., Dewhirst, F.E., Defining the normal bacterial flora of the oral cavity (2005) J Clin Microbiol, 43, pp. 5721-5732Basso, F.G., Pansani, T.N., Turrioni, A.P.S., Bagnato, V.S., Hebling, J., de Souza Costa, C.A., In vitro wound healing improvement by low-level laser therapy application in cultured gingival fibroblasts (2012) Int J Dent, , [Epub ahead of print. DOI: 10.1155/2012/719452]Wiegand, C., Hipler, U., Methods for the measurement of cell and tissue compatibility including tissue regeneration process (2008) GMS Krankenhhyg Interdiszip, 3, pp. 1863-5245Basso, F.G., Oliveira, C.F., Kurachi, C., Hebling, J., de Souza Costa, C.A., Biostimulatory effect of low-level laser therapy on keratinocytes in vitro (2013) Lasers Med Sci, 28, pp. 367-374De Souza Costa, C.A., Duarte, P.T., de Souza, P.P., Giro, E.M., Hebling, J., Cytotoxic effects and pulpal response caused by a mineral trioxide aggregate formulation and calcium hydroxide (2008) Am J Dent, 21, pp. 255-261Oliveira, C.F., Basso, F.G., Lins, E.C.C., Kurachi, C., Hebling, J., Bagnato, V.S., Increased viability of odontoblast-like cells subjected to low-level laser irradiation (2010) Laser Phys, 20, pp. 1659-1666Read, S.M., Northcote, D.H., Minimization of variation in the response to different proteins of the Coomassie blue G dye-binding assay for protein (1981) Anal Biochem, 116, pp. 53-64Oliveira, C.F., Basso, F.G., Lins, E.C., Kurachi, C., Hebling, J., Bagnato, V.S., In vitro effect of low-level laser on odontoblast-like cells (2011) Laser Phys Lett, 8, pp. 155-163Simon, M.J.K., Niehoff, P., Kimming, B., Wiltfang, J., Açil, Y., Expression profile and synthesis of different collagen types I, II III and V of human gingival fibroblasts, osteoblasts, ans SaOs-2 cells after bisphosphonate treatment (2010) Clin Oral Investig, 14, pp. 51-58Migliorati, C.A., Siegel, M.A., Elting, L.S., Bisphosphonate-associated osteonecrosis: A long-term complication of bisphosphonate treatment (2006) Lancet Oncol, 7, pp. 508-514Werner, S., Krieg, T., Smola, H., Keratinocyte-fibroblast interactions in wound healing (2007) J Investigative Dermatol, 127, pp. 998-1008Ravosa, M.J., Ning, J., Liu, Y., Stack, M.S., Bisphosphonate effects on the behavior of oral epithelial cells and oral fibroblasts (2011) Arch Oral Biol, 56, pp. 491-49

In Vitro Effect Of Low-level Laser Therapy On Typical Oral Microbial Biofilms

The aim of this study was to evaluate the effect of specific parameters of low-level laser therapy (LLLT) on biofilms formed by Streptococcus mutans, Candida albicans or an association of both species. Single and dual-species biofilms - SSB and DSB - were exposed to laser doses of 5, 10 or 20 J/cm 2 from a near infrared InGaAsP diode laser prototype (LASERTable; 780 ± 3 nm, 0.04 W). After irradiation, the analysis of biobilm viability (MTT assay), biofilm growth (cfu/mL) and cell morphology (SEM) showed that LLLT reduced cell viability as well as the growth of biofilms. The response of S. mutans (SSB) to irradiation was similar for all laser doses and the biofilm growth was dose dependent. However, when associated with C. albicans (DSB), S. mutans was resistant to LLLT. For C. albicans, the association with S. mutans (DSB) caused a significant decrease in biofilm growth in a dose-dependent fashion. The morphology of the microorganisms in the SSB was not altered by LLLT, while the association of microbial species (DSB) promoted a reduction in the formation of C. albicans hyphae. LLLT had an inhibitory effect on the microorganisms, and this capacity can be altered according to the interactions between different microbial species.226502510Marques, M.M., Pereira, A.N., Fujihara, N.A., Nogueira, F.N., Eduardo, C.P., Effect of low-power laser irradiation on protein synthesis and ultrastructure of human gingival fibroblasts (2004) Lasers Surg Med, 34, pp. 260-265Damante, C.A., de Micheli, G., Miyagi, S.P.H., Feist, I.S., Marques, M.M., Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts (2009) Lasers Med Sci, 24, pp. 885-891Moritz, A., Schoop, U., Goharkhay, K., Schauer, P., Doertbudak, O., Wernisch, J., Treatment of periodontal pockets with a diode laser (1998) Lasers Surg Med, 22, pp. 302-311Nussbaum, E.L., Lilge, L., Mazzulli, T., Effects of 630-, 660-, 810-, and 905-nm laser irradiation delivering radiant exposure of 1-50 J/cm 2 on three species of bacteria in vitro (2002) J Clin Laser Med Surg, 20, pp. 325-333Nussbaum, E.L., Lilge, L., Mazzulli, T., Effects of low-level laser therapy (LLLT) of 810 nm upon in vitro growth of bacteria: Relevance of irradiance and radiant exposure (2003) J Clin Laser Med Surg, 21, pp. 283-290Lino, M.D.M.C., Carvalho, F.B., Oliveira, L.R., Magalhães, E.B., Pinheiro, A.L.B., Ramalho, L.M.P., Laser phototherapy as a treatment for radiotherapy-induced oral mucositis (2011) Braz Dent J, 22, pp. 162-165Maver-Biscanin, M., Mravak-Stipetic, M., Jerolimov, V., Biscanin, A., Fungicidal effect of diode laser irradiation in patients with denture stomatitis (2004) Lasers Surg Med, 35, pp. 259-262Dworkin, M., Endogenous photosensitization in a carotinoidless mutant of Rhodopseudomonas speroides (1958) J Gen Physiol, 43, pp. 1099-1112Rosenberg, B., Kemeny, G., Switzer, R.C., Hamilton, T.C., Quantitative evidence for protein denaturation as the cause of thermal death (1971) Nature, 232, pp. 471-473Krespi, Y.P., Kizhner, V., Nistico, L., Hall-Stoodley, L., Stoodley, P., Laser disruption and killing of methicillin-resistant Staphylococcus aureus biofilms (2011) Am J Otolaryngol, 32, pp. 198-202Shirtliff, M.E., Peters, B.M., Jabra-Rizk, M.A., Cross-kingdom interactions: Candida albicans and bacteria (2009) FEMS Microbiol Lett, 299, pp. 1-8Pereira-Cenci, T., Deng, D.M., Kraneveld, E.A., Manders, E.M.M., del Bel, C.A.A., ten Cate, J.M., The effect of Streptococcus mutans and Candida glabrata on Candida albicans biofilms formed on different surfaces (2008) Arch Oral Biol, 53, pp. 755-764Marsh, P.D., Microbial ecology of dental plaque and its significance in health and disease (1994) Adv Dent Res, 8, pp. 263-271Karkowska-Kuleta, J., Rapala-Kozik, M., Kozik, A., Fungi pathogenic to humans: Molecular bases of virulence of Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus (2009) Acta Biochim Pol, 56, pp. 211-224Thein, Z.M., Samaranayake, Y.H., Samaranayake, L.P., Dietary sugars, serum and the biocide chlorhexidine digluconate modify the population and structural dynamics of mixed Candida albicans and Escherichia coli biofilms (2007) APMIS, 115, pp. 1241-1251Kwieciński, J., Eick, S., Wójcik, K., Effects of tea tre (Melaleuca alternifolia) oil on Staphylococcus aureus in biofilms and stationary phase (2009) Int J Antimicrob Agents, 33, pp. 343-347Wang, Z.C., Fan, L.Y., Jiang, J.Q., Cai, W., Ding, Y., Study on the counting of Streptococcus mutans, Streptococcus sanguis, Haemophilus actinomycetemcomitans by methyl thiazolyl tetrazolium colorimetric method (2010) Hua Xi Kou Qiang Yi Xue Za Zhi, 28, pp. 306-310Nguyen, P.T.M., Abranches, J., Phan, T., Marquis, R.E., Repressed respiration of oral Streptococci grow in biofilms (2002) Curr Microbiol, 44, pp. 262-266Singleton, S., Treloar, R., Warren, P., Watson, G.K., Hodgson, R., Allison, C., Methods for microscopic characterization of oral biofilms: Analysis of colonization, microstructure, and molecular transport phenomena (1997) Adv Dent Res, 11, pp. 133-149Jarosz, L.M., Deng, D.M., van der Mei, H.C., Crielaard, W., Krom, B.P., Streptococcus mutans competence-stimulating peptide inhibits Candida albicans hypha formation (2009) Eukaryot Cell, 8, pp. 1658-1664Dortbudak, O., Haas, R., Bernhart, T., Mailath-Pokorny, G., Lethal photosensitization for decontamination of implant surface in the treatment of peri-implantitis (2001) Clin Oral Implant Res, 12, pp. 104-10

d-alpha Correlation functions and collective motion in Xe+Au collisions at E/A=50 MeV

The interplay of the effects of geometry and collective motion on d-$\alpha$ correlation functions is investigated for central Xe+Au collisions at E/A=50 MeV. The data cannot be explained without collective motion, which could be partly along the beam axis. A semi-quantitative description of the data can be obtained using a Monte-Carlo model, where thermal emission is superimposed on collective motion. Both the emission volume and the competition between the thermal and collective motion influence significantly the shape of the correlation function, motivating new strategies for extending intensity interferometry studies to massive particles.Comment: Accepted for publication on Physics Letters

Balanço do nitrogênio e fósforo em solo com cultivo orgânico de hortaliças após a incorporação de biomassa de guandu.

Os objetivos deste trabalho foram avaliar os efeitos de faixas de guandu (Cajanus cajan) e da incorporação da biomassa proveniente de sua poda na fertilidade do solo e na produtividade de três hortaliças sob cultivo orgânico. O delineamento usado foi de blocos casualizados completos em esquema de parcelas subsubdivididas com três repetições. As produtividades de beterraba, cenoura e feijão-de-vagem não foram afetadas pelos tratamentos. Nas parcelas onde não houve incorporação da biomassa de guandu, o balanço de nitrogênio no sistema foi negativo, ao passo que com a incorporação, esse balanço foi positivo. Embora tenha ocorrido balanço positivo para o fósforo nas parcelas sem a incorporação de biomassa de guandu, houve um aumento significativo na absorção desse elemento pelas hortaliças quando o material foi incorporado. O sistema de cultivo em aléias de guandu pode representar uma prática vantajosa para os produtores orgânicos, por contribuir na manutenção da fertilidade do solo

Vascular Flora Of The Legado Das Águas, Reserva Votorantim, Municipalities Of Tapiraí, Miracatú And Juquiá, São Paulo, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)We present a list of vascular plants found in Legado das Águas, Reserva Votorantim, a private reserve having an area of approximately 35,000 ha that spans the municipalities of Tapiraí, Miracatú, and Juquiá, in the state of São Paulo, Brazil. The reserve is part of a complex of state-protected areas including Alto Ribeira Touristic State Park, Intervales State Park, Carlos Botelho State Park, Jurupará State Park, and Serra do Mar State Park. Together, these form an important and large area of continuous and well-preserved forest. Our study, which involved two main floristic surveys in March/April 2013 and April 2014, recorded 768 species, representing 131 families and 432 genera. The majority of species were angiosperms (619) representing 106 families and 370 genera. There were also two species of gymnosperms belonging to two genera in separate families; 147 species of ferns and lycophytes belonging to 23 families and 60 genera. In demonstrating the presence of almost 800 species of plants, of which 16 are threatened, our study highlights the importance of floristic surveys and the critical role of private protected areas in managing and preserving native flora. © 2016 Check List and Authors.1262011/22923-8, FAPESP, Fundação de Amparo à Pesquisa do Estado de São Paulo2015/9444-4, FAPESP, Fundação de Amparo à Pesquisa do Estado de São PauloFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP