Effect of frozen period on the chemical, microbiological and sensory quality of frozen tilapia fish (Sarotherodun galiaenus)

The study was designed to investigate the effect of duration of frozen storage on the chemical, microbiological and sensory profile of Tilapia fish (Sarotherodun galiaenus) collected from a research pond of the Agricultural Development Project, Akure, Nigeria and were subjected to sixty days of frozen storage and analyzed at intervals of ten days. Protein content (%) ranges from 43.70 &#177 1.17-60.65 &#177 2.40. Protein decreases with increasing duration of frozen storage with the fresh samples (not frozen) having the highest protein content (60.65 ± 2.40) while the least (43.70 &#1771.17) was recorded for fish samples that were frozen for sixty days. Similar results were obtained for the fat content (%) where the highest fat content (9.72 &#177 0.25) was recorded for the fresh samples and the least value was recorded for those stored for sixty days. Ash content (%) and moisture content (%) do not show any significant change during storage. Mineral composition (Fe, Ca, Mg, P and Zn in mg/100g) and iodine content (µg/100g) of the samples showed a slight change with respect to duration of storage. pH values ranges between 5.20-6.90 while the total coliform count range was between 3.0 x103-7.5 x 106 with increasing values, as the duration of torage increases. Sensory evaluation of the fish samples on storage revealed that quality of the fish samples with respect to taste decreases with increasing duration of storage with the best quality (texture, odor and color) when freshly prepared; and that better quality during storage is obtainable during the first ten days of storage. These, by implication simply mean that fish should be stored for a short period of time to retain the taste, and provide both the protein and fat at optimal level. Key words: Frozen period, chemical, microbiological, sensory quality, tilapia fish.African Journal of Biotechnology Vol. 4 (8), pp. 852-85

Farmers' perception of the epidemic of African Swine Fever in Nigeria

Livestock diseases constitute a great threat to protein availability in Nigeria. It is thus necessary to examine how much farmers know about some deadly diseases prevalent in their stock as it would afford the farmer a timely re-adjustment to prevent foreseeable losses. The focus of this study was to determine farmers' level of awareness of African Swine Fever (ASF) in Agege Area of Lagos State, Nigeria. Primary data were collected with the use of a questionnaire administered to one hundred and twenty (120) respondents selected using the purposive sampling technique. Twenty-seven (27) pig farms were also visited to obtain on-farm data on pig mortality during the ASF epidemic. It was revealed that there was inadequate awareness of the early symptoms and characteristic signs of ASF among the respondents. As such mortality of about 95 percent was recorded The Chi square analysis showed no significant relationship between farmers' level of awareness of ASF and the location of their pig farms. There was also no significant relationship between pig stock population and farmers ' contact with Extension agents. The study then concluded that extension services to pig farmers are currently inadequate. It therefore recommends that it be developed to ensure institutional support in cases of epidemic disease outbreaks. More so, possible ways should be sought to ensure a steady flow of agricultural information from the research institutes and universities to the ultimate users. Preventive measures should however be taught to farmers to avoid the incidence of future disease outbreaks. Lastly, pig farmers are also advised to form associations that could serve as a pressure group in such cases of sector specific emergencies.Keywords: Perception, epidemic disease, African swine feve

Genetic, Reproductive and Hematological Toxicity Induced in Mice Exposed to Leachates from Petrol, Diesel and Kerosene Dispensing Sites

Background. With a population of over 165,000,000, growing at an average rate of 2.7% per annum and an economic growth rate of about 5.7% in the past five years, the market for refined petroleum products in Nigeria is growing. As a result, the number of filling stations is increasing. Objectives. The present study evaluated the reproductive and genetic toxicity of simulated leachate of soil from petrol, diesel and kerosene dispensing sites in a filling station using the murine sperm abnormality test, sperm count and bone marrow micronucleus assay. Methods. Simulated leachate of soil collected from petrol, diesel and kerosene dispensing sites in a filling station was intraperitoneally administered to mice at different concentrations. Bone marrow micronucleus assay was carried out after 5-days exposure, while sperm morphology assay was carried out 35 days from the first day of exposure. Alterations to hematological parameters were evaluated and physico-chemical analysis of the leachate samples was also carried out. Results. The results showed a significant (p<0.05) concentration-dependent increase in abnormal sperm cells and decrease in mean sperm count in all the samples tested. Increased induction of micronucleated polychromatic erythrocytes was observed in the exposed mice. Hematological analysis showed a significant (p<0.05) increase in the values of white blood cell count (WBC), lymphocytes, neutrophils, monocytes, eosinophils and mean corpuscular volume (MCV), while a significant (p<0.05) reduction in basophils, hemoglobin, mean corpuscular hemoglobin (MCH), packed cell volume and mean corpuscular hemoglobin concentration (MCHC) values were observed. Discussion. In the present study, simulated leachates from soil obtained from petrol, diesel and kerosene dispensing sites were shown to cause genomic disruptions in germ and somatic cells, and hematotoxicity in an animal model. These observed reproductive, genetic and hemato-toxicities are believed to be caused by the presence of lead, copper, mercury, polycyclic aromatic hydrocarbons, and benzene in the samples. Conclusions. This study showed the negative impact of petroleum products in the contamination of soil, with a capability of inducing genetic damage in somatic and germ cells of exposed plants and animals. Competing Interests. The authors declare no financial competing interests. Ethics Approval. The study was approved by the ethical committee of the Federal University of Technology, Akure, Ondo State, Nigeria

Effect of different levels of honey on physiological, growth and carcass traits of broiler chickens during dry season

The objective of this trial was to investigate the effect of different levels of honey in drinking water on the responses of broiler chickens during dry season in hot humid tropics. Three hundred Oba Marshall broiler chicks were used for this study. The day-old chicks were randomly assigned to five treatment groups with each treatment having four replicates of 15 birds. The first 4 treatment groups were daily supplied drinking water without honey (0H), with 20 ml (20H), 40 ml (40H) and 60 ml (60H) of honey per liter of water. The fifth group received drinking water supplemented with 500 mg vitamin C per liter of water (C). Records on daily weight gain (WG) and feed intake (FI) were taken. Feed conversion ratio (FCR) was calculated as the ratio of gain to feed consumed. The supplementation of honey in the drinking water for broiler chickens in 60H during hot dry season in the hot humid tropics improved some stress indices, body weight gain and relative spleen weight.</p

Implementation of data triangulation and dashboard development for COVID-19 vaccine adverse event following immunisation (AEFI) data in Nigeria

Nigeria began administering COVID-19 vaccines on 5 March 2021 and is working towards the WHO’s African regional goal to fully vaccinate 70% of their eligible population by December 2022. Nigeria’s COVID-19 vaccination information system includes a surveillance system for COVID-19 adverse events following immunisation (AEFI), but as of April 2021, AEFI data were being collected and managed by multiple groups and lacked routine analysis and use for action. To fill this gap in COVID-19 vaccine safety monitoring, between April 2021 and June 2022, the US Centers for Disease Control and Prevention, in collaboration with other implementing partners led by the Institute of Human Virology Nigeria, supported the Government of Nigeria to triangulate existing COVID-19 AEFI data. This paper describes the process of implementing published draft guidelines for data triangulation for COVID-19 AEFI data in Nigeria. Here, we focus on the process of implementing data triangulation rather than analysing the results and impacts of triangulation. Work began by mapping the flow of COVID-19 AEFI data, engaging stakeholders and building a data management system to intake and store all shared data. These datasets were used to create an online dashboard with key indicators selected based on existing WHO guidelines and national guidance. The dashboard went through an iterative review before dissemination to stakeholders. This case study highlights a successful example of implementing data triangulation for rapid use of AEFI data for decision-making and emphasises the importance of stakeholder engagement and strong data governance structures to make data triangulation successful