DISTRIBUTED INTELLIGENT SPECTRUM MANAGEMENT IN COGNITIVE RADIO AD HOC NETWORKS

The rapid growth of the number of wireless devices has brought an exponential increase in the demand of the radio spectrum. However, according to the Federal Communications Commission (FCC), almost all the radio spectrum for wireless com- munications has already been allocated. In addition, according to FCC, up to 85% of the allocated spectrum is underutilized due to the current fixed spectrum alloca- tion policy. To alleviate the spectrum scarcity problem, FCC has suggested a new paradigm for dynamically accessing the allocated spectrum. Cognitive radio (CR) technology has emerged as a promising solution to realize dynamic spectrum access (DSA). With the capability of sensing the frequency bands in a time and location- varying spectrum environment and adjusting the operating parameters based on the sensing outcome, CR technology allows an unlicensed user to exploit the licensed channels which are not used by licensed users in an opportunistic manner. In this dissertation, distributed intelligent spectrum management in CR ad hoc networks is explored. In particular, four spectrum management issues in CR ad hoc networks are investigated: 1) distributed broadcasting in CR ad hoc networks; 2) distributed optimal HELLO message exchange in CR ad hoc networks; 3) distributed protocol to defend a particular network security attack in CR ad hoc networks; and 4) distributed spectrum handoff protocol in CR ad hoc networks. The research in this dissertation has fundamental impact on CR ad hoc network establishment, net- work functionality, network security, and network performance. In addition, many of the unique challenges of distributed intelligent spectrum management in CR ad hoc networks are addressed for the first time in this dissertation. These challenges are extremely difficult to solve due to the dynamic spectrum environment and they have significant effects on network functionality and performance. This dissertation is essential for establishing a CR ad hoc network and realizing networking protocols for seamless communications in CR ad hoc networks. Furthermore, this dissertation provides critical theoretical insights for future designs in CR ad hoc networks

Modeling, simulation and analysis of the reaction field for electrostatic interactions in aqueous solution

How to deal with the long-range electrostatic interactions theoretically and computationally has been well studied due to their importance in biological processes and time consuming summations in computer simulations. The main focus of our research has been on the design and application of a new type of hybrid model that combines both the explicit and implicit solvent models using a reaction field (RF) approach, for accurate and efficient electrostatic calculations. This hybrid model, named as Image Charge Solvation Model (ICSM), replaces an infinite Coulomb summation by two finite sums over direct interactions plus image charges for RF. To characterize the ICSM, the electrostatic torques and forces using different model parameters are compared through various histogram distributions. The contributions of RF are 20% and 2% of the total electrostatic torques and forces, respectively, suggesting that the main effect of RF is to maintain the orientation of water dipoles in the solution. Considering systematic artifacts of the discontinuous dielectric constant at the edge of the cavity, we modified the image charge formula in an optimal way to better account for the continuously changing dielectric profile near the boundary, which provides a computational procedure to determine the most accurate RF possible for a specified water model. The Periodic Boundary Conditions (PBC) in ICSM reduces the size of the productive region and introduces unphysical correlations between ions in ionic solution. With combination of finite boundary conditions, mean field theory for short-range forces and multiple constraint forces applied to water molecules in a buffer layer, bulk water properties are maintained without problems from imaged ions in a much bigger usable region than before. To summarize, the results presented in this work provide a complete characterization, optimization and improvement of the ICSM for electrostatic calculations

A new approach to Mandarin Chinese lyric diction in choral music

Chinese choral repertoire is less performed in non-native Chinese-speaking countries, specifically in the United States. This repertoire is abundant. One reason for the underrepresentation of this repertoire in choral concerts is that Chinese composers are typically self-published and their information is usually in Chinese. Therefore, their reputations and achievements are mainly celebrated in China. However, the accurate and consistent pronunciation of the lyrics by non-native speakers remains the most significant barrier. The purpose of this study is to provide a new approach to Mandarin Chinese lyric diction in choral music by combining the Pin Yin linguistic system and the standard Western European International Phonetic Alphabet system most commonly used for diction studies of languages such as Italian, German, French, and English. In this document, Chapter I is a brief overview outlining the reasons for the absence of Chinese choral music in performances by non-native Chinese-speaking countries, especially in the United States. Chapter II introduces the general view of the Chinese language, including a brief history of the written characters, spoken language, and three linguistic systems for the characters, and finally, focuses on the most recent and commonly used Pin Yin linguistic system. An in-depth description of the sounds of Mandarin Chinese alphabetic letters and their IPA equivalents is the content of Chapters III, IV, and V: Consonants in Chapter III; Vowels, Diphthongs, and Nasal Finals in Chapter IV; and Glides in Chapter V. The focus of Chapter VI is a demonstration of the application, artistic, and stylistic decisions made in three choral pieces utilizing the Mandarin Chinese language. Lastly, Chapter VII summarizes the information in the preceding chapters, which is the document’s Conclusion

Structural insight into SUMO chain recognition and manipulation by the ubiquitin ligase RNF4

The small ubiquitin-like modifier (SUMO) can form polymeric chains that are important signals in cellular processes such as meiosis, genome maintenance and stress response. The SUMO-targeted ubiquitin ligase RNF4 engages with SUMO chains on linked substrates and catalyses their ubiquitination, which targets substrates for proteasomal degradation. Here we use a segmental labelling approach combined with solution nuclear magnetic resonance (NMR) spectroscopy and biochemical characterization to reveal how RNF4 manipulates the conformation of the SUMO chain, thereby facilitating optimal delivery of the distal SUMO domain for ubiquitin transfer

The structure of a resuscitation-promoting factor domain from Mycobacterium tuberculosis shows homology to lysozymes

Resuscitation-promoting factor (RPF) proteins reactivate stationary-phase cultures of (G+C)-rich Gram-positive bacteria including the causative agent of tuberculosis, Mycobacterium tuberculosis. We report the solution structure of the RPF domain from M. tuberculosis Rv1009 (RpfB) solved by heteronuclear multidimensional NMR. Structural homology with various glycoside hydrolases suggested that RpfB cleaved oligosaccharides. Biochemical studies indicate that a conserved active site glutamate is important for resuscitation activity. These data, as well as the presence of a clear binding pocket for a large molecule, indicate that oligosaccharide cleavage is probably the signal for revival from dormancy

Opening Doorways: Connecting Petersburg Students to the World of Health Science Professions

This project creates a strategy to build a pipeline that begins in elementary school and is reinforced through middle and high school. This early exposure and understanding of health career opportunities is needed. Too many communities in Virginia experience critical health care inequities in services and information. To address those inequities, we need to open doorways to investigate health careers. By inspiring students from these communities to enter medical and health sciences professions, there is better representation and services to these underrepresented communities. VCU not only can provide exposure to various health careers, experiential learning and mentoring on how to navigate through academia into a chosen field but also strength community engaged

Enterobius vermicularis in the male urinary tract: a case report

Enterobius vermicularis is an intestinal nematode of humans. Adults usually have low worm burdens and are asymptomatic. Ectopic infections in the pelvic area or urinary tract rarely occur in women. We report a case of the patient with mild voiding difficulties such as urgency, frequency, nocturia, dysuria, mild low back pain or perineal discomfort. The patient's prostatic secretions showed a large number of inflammatory cells and several eggs. The size and the shape of the eggs identified them as a group of E. vermicularis. On examination we found a soft palpable material which was 5 mm diameter in size and spherical shape. Palpation gave the impression of a tissue than a stone. An incision was performed and a 4 mm long living worm was found. The microscopic examination identified the worm as E- vermicularis. It is an extremely rare manifestation of enterobius vermicularis infection since an intestinal-breeding worm is rarely found in the male genital tract

A novel hybrid engineering methodology to enhance collaboration during the design and development of meta product services

Since the Product-Service System (PSS) is accompanied by a paradigm shift towards the sustainability of the production process, it demands an advanced level of collaboration throughout the design and development process. This poses new challenges to enhance collaboration among cross-sectional, multi-disciplinary design teams in order to incorporate a variety of interdisciplinary services in a complex service-oriented system. Therefore, a new collaboration methodology is needed to reduce barriers to collaboration in a cross- organizational structure domain. This research work aims at highlighting the collaboration challenges that Meta-Product design teams face and also proposes a novel methodology to enhance collaboration among multi-disciplinary teams. The proposed methodology is a hybrid engineering model designed by integrating an Information Delivery Manual approach with a traditional software development process along with three interlinked collaboration services

Long Noncoding RNA Expression Signatures of Metastatic Nasopharyngeal Carcinoma and Their Prognostic Value

Long noncoding RNAs (lncRNAs) have recently been found to play important roles in various cancer types. The elucidation of genome-wide lncRNA expression patterns in metastatic nasopharyngeal carcinoma (NPC) could reveal novel mechanisms underlying NPC carcinogenesis and progression. In this study, lncRNA expression profiling was performed on metastatic and primary NPC tumors, and the differentially expressed lncRNAs between these samples were identified. A total of 33,045 lncRNA probes were generated for our microarray based on authoritative data sources, including RefSeq, UCSC Knowngenes, Ensembl, and related literature. Using these probes, 8,088 lncRNAs were found to be significantly differentially expressed (2-fold). To identify the prognostic value of these differentially expressed lncRNAs, four lncRNAs (LOC84740, ENST00000498296, AL359062, and ENST00000438550) were selected; their expression levels were measured in an independent panel of 106 primary NPC samples via QPCR. Among these lncRNAs, ENST00000438550 expression was demonstrated to be significantly correlated with NPC disease progression. A survival analysis showed that a high expression level of ENST00000438550 was an independent indicator of disease progression in NPC patients (). In summary, this study may provide novel diagnostic and prognostic biomarkers for NPC, as well as a novel understanding of the mechanism underlying NPC metastasis and potential targets for future treatment

Protein interactions in Xenopus germ plasm RNP particles

Hermes is an RNA-binding protein that we have previously reported to be found in the ribonucleoprotein (RNP) particles of Xenopus germ plasm, where it is associated with various RNAs, including that encoding the germ line determinant Nanos1. To further define the composition of these RNPs, we performed a screen for Hermes-binding partners using the yeast two-hybrid system. We have identified and validated four proteins that interact with Hermes in germ plasm: two isoforms of Xvelo1 (a homologue of zebrafish Bucky ball) and Rbm24b and Rbm42b, both RNA-binding proteins containing the RRM motif. GFP-Xvelo fusion proteins and their endogenous counterparts, identified with antisera, were found to localize with Hermes in the germ plasm particles of large oocytes and eggs. Only the larger Xvelo isoform was naturally found in the Balbiani body of previtellogenic oocytes. Bimolecular fluorescence complementation (BiFC) experiments confirmed that Hermes and the Xvelo variants interact in germ plasm, as do Rbm24b and 42b. Depletion of the shorter Xvelo variant with antisense oligonucleotides caused a decrease in the size of germ plasm aggregates and loosening of associated mitochondria from these structures. This suggests that the short Xvelo variant, or less likely its RNA, has a role in organizing and maintaining the integrity of germ plasm in Xenopus oocytes. While GFP fusion proteins for Rbm24b and 42b did not localize into germ plasm as specifically as Hermes or Xvelo, BiFC analysis indicated that both interact with Hermes in germ plasm RNPs. They are very stable in the face of RNA depletion, but additive effects of combinations of antisense oligos suggest they may have a role in germ plasm structure and may influence the ability of Hermes protein to effectively enter RNP particles