Hepatoprotective effect of Phytosome Curcumin against paracetamol-induced liver toxicity in mice

Curcuma longa, which contains curcumin as a major constituent, has been shown many pharmacological effects, but it is limited using in clinical due to low bioavailability. In this study, we developed a phytosome curcumin formulation and evaluated the hepatoprotective effect of phytosome curcumin on paracetamol induced liver damage in mice. Phytosome curcumin (equivalent to curcumin 100 and 200 mg/kg body weight) and curcumin (200 mg/kg body weight) were given by gastrically and toxicity was induced by paracetamol (500 mg/kg) during 7 days. On the final day animals were sacrificed and liver function markers (ALT, AST), hepatic antioxidants (SOD, CAT and GPx) and lipid peroxidation in liver homogenate were estimated. Our data showed that phytosome has stronger hepatoprotective effect compared to curcumin-free. Administration of phytosome curcumin effectively suppressed paracetamol-induced liver injury evidenced by a reduction of lipid peroxidation level, and elevated enzymatic antioxidant activities of superoxide dismutase, catalase, glutathione peroxidase in mice liver tissue. Our study suggests that phytosome curcumin has strong antioxidant activity and potential hepatoprotective effects

On the Interference Alignment Designs for Secure Multiuser MIMO Systems

In this paper, we propose two secure multiuser multiple-input multiple-output transmission approaches based on interference alignment (IA) in the presence of an eavesdropper. To deal with the information leakage to the eavesdropper as well as the interference signals from undesired transmitters (Txs) at desired receivers (Rxs), our approaches aim to design the transmit precoding and receive subspace matrices to minimize both the total inter-main-link interference and the wiretapped signals (WSs). The first proposed IA scheme focuses on aligning the WSs into proper subspaces while the second one imposes a new structure on the precoding matrices to force the WSs to zero. When the channel state information is perfectly known at all Txs, in each proposed IA scheme, the precoding matrices at Txs and the receive subspaces at Rxs or the eavesdropper are alternatively selected to minimize the cost function of an convex optimization problem for every iteration. We provide the feasible conditions and the proofs of convergence for both IA approaches. The simulation results indicate that our two IA approaches outperform the conventional IA algorithm in terms of average secrecy sum rate.Comment: Updated version, updated author list, accepted to be appear in IEICE Transaction

Optimal control of switched capacitor banks in Vietnam distribution network using integer genetic algorithm

In distribution network, power and energy losses can be reduced by using switched capacitor banks. The capacitor banks can be switched on or off based on voltage profile or power factor or using timers. Due to variation of load, it is necessary to control the capacitor banks switching in function of load curve. This paper presents the application of an integer genetic algorithm to determine the optimal number of banks corresponding with hourly load to minimize total active power losses of distribution feeders. The problem constraints include voltage profile and heat conditions which are taken into account to the objective function by a penalty function. In this application, the structure of chromosomes is a set of numbers of the capacitor banks hourly connected to the grid. The proposed formulation is validated by a feeder. The result shows that in some cases, the active power losses at maximum compensation are greater than the ones of optimal control compensation, and the voltage reaches a higher level than the maximum voltage limit. The optimal control of switched capacitor banks can reduce power and energy losses as well as ensure maximum voltage profile within the limit

Detection of genetically modified organisms (GMOs) using molecular techniques in food and feed samples from Malaysia and Vietnam

Food labeling in accordance with Novel Food Regulation has been enforced in the European Community since 1997 with a series of updated legislations namely, EC/258/97, EC/1139/98, EC/49/2000, EC/50/2000 and EC/1829/2003. Guidelines and labeling regulations for the use of GMOs materials in food and feed products has also been introduced in Malaysia and Vietnam. Therefore, the demand for the establishment and development of a robust and rapid operation procedure for GMO detection has increased recently in both countries. The procedure of GMO detection emphasizes not only on detection tests but also on confirmation assays. This study employed PCR technology for detection and direct DNA sequencing for confirmation procedures respectively. The results demonstrated for the first time the presence of GM plants with glyphosate-resistant trait led by the control of P35S promoter and NOS terminator in either Malaysian or Vietnamese feed with high frequency (20 positive samples out of 24 analyzed samples). The P35S promoter, EPSPS gene and NOS terminator sequences obtained showed some mutations on single-stranded and double-stranded targeted sequences caused by single nucleotide insertion or single nucleotide changes. These results reinforce the need for development of detection procedures to comply with food/feed labeling system

Comparison of DNA extraction efficiencies using various methods for the detection of genetically modified organisms (GMOs)

The ability to detect the presence of transgenes in crop-derived foods depends on the quantity and quality of DNA obtained from a product to be analyzed. The efficiency of DNA extraction protocols differs due to the nature of each food product. In this paper, we described two main DNA extraction protocols and their modifications that have been applied and evaluated for DNA extraction from raw and processed food as well as animal feed. The yield and quality for five categories of food and feed samples namely, raw soybean, raw maize, animal feed, smooth tofu and soymilk are discussed. The statistical interaction analyses showed that the cetyltrimethyl ammonium bromide (CTAB) method was proven to be the best method to extract DNA from raw soybean, maize and animal feed samples which not only obtained high DNA yield of 32.7, 28.4 and 33.4 ng DNA/mg sample respectively, but also produced high quality DNA with the absorbance A260/A280 ratio of 1.9, 1.9 and 2.0, respectively. These DNA were suitable for PCR amplification which produced a 164 bp DNA fragment of the lectin gene from soybean, and a 277 bp DNA fragment of the zein gene from maize. In the processed food category, the Wizard isolation method was found to be the best for the extraction of DNA from smooth tofu and soymilk with the yield of 13.2 and 3.4 ng DNA/mg sample, and the quality of the DNA at the absorbance A260/A280 ratio ranged from 1.9 to 1.7. These DNA were successfully amplified using primers specific to the lectin gene of soybean

Effect of Different Types of Liquid Natural Rubbers on the Modification of DGEBA-Based Epoxy Resin

Diglycidyl ether of bisphenol A (DGEBA)-based epoxy and varying content of hydroxyl terminated liquid natural rubber (HTNR) or carboxyl-terminated liquid natural rubber (CTNR) were cured using an aromatic amine hardener. The ultimate aim of the study was to modify the brittle epoxy matrix by liquid rubber to improve the toughness characteristics. Tensile, flexural, and fracture toughness behaviors of neat as well as modified networks have been studied to observe the effect of different types of  liquid natural rubber modification. The morphological evolution of the toughened networks was examined by scanning electron microscope (SEM), and the observations were used effectively to explain the impact properties of the network having varying content of liquid natural rubbers. The results showed that the impact resistance of both HTNR-modified DGEBA and CTNR-modified DGEBA was superior to that of the pure epoxy resin, among which the impact resistance of CTNR-modified DGEBA was better than that of HTNR-modified DGEBA. For all the composites with HTNR or CTNR, the impact resistance increased with elastomer concentration up to 15.0 parts per hundred parts of resin (phr). Higher concentration of the elastomers resulted in larger particles and gave lower impact values. These results allow the conclusion that 15 phr is the maximum content of HTPB or CTNR that might be added in DGEBA composites for a positive effect upon the impact strength

HORIZONTALLY PLASMON HYBRIDIZATION ON SYMMETRIC-BREAKING METAMATERIALS

In this report, we present a study on the fundamental negative-permeability metamaterials, named as the cut-wire-pair structure. The physics of the cut-wire-pair metamaterial is interpreted using the electromagnetic analog of molecular-orbital theory. It is shown that a symmetric-breaking cut-wire-pair metamaterial is horizontally plasmon-hybridized, leading to an additional magnetic resonance beyond the conventional one. The transmission spectra and the induced energy distributions are performed to demonstrate our prediction

STUDY ON INSPECTION OF THE PUTATIVE HYBRIDS BETWEEN TRACK-LACKING WILD MALE GAUR (BOS GAURUS) AND DOMESTIC FEMALE COW (BOS TAURUS) IN PHUOC BINH NATIONAL PARK, VIETNAM

Hybridization between wild and domestic bovine occurs widely due to the overlapping of the natural habitat of the wild and human farm animals. Due to the loss of habitat, the number of wild gaur (Bos gaurus) in Vietnam was in serious decline. Since 2009, a male wild gaur (Bos gaurus) has appeared and incorporated with female domestic cows (Bos taurus) in buffer zone Phuoc Binh National Park, on the border with Lam Dong and Ninh Thuan provinces. Then, several calves were born that carried some traits of wild gaur including physical characteristics and behaviors. These calves were supposed to be offspring between wild gaur and domestic cows. In previous study, the karyotypes of putative calves were identified with 2n = 58 and non-homologous chromosome 28 and 29 in these hybrids. In this study, we characterized the putative hybrids between track-lacking Bos gaurus and Bos taurus for breeding and preservation using Cytochrome b analysis, microsatellites and a novel marker growth hormone factor 1 (POU1F1). Cytochrome b analysis indicated the maternal lineage of the putative hybrids, with 100% nucleotide sequence identity. Microsatellite BM861 and sequence of ZFY gene region reveal Bos taurus chromosome Y origin was among the male putative hybrids. Importantly, the analysis of POU1F1 gene sequence on 1 chromosome showed efficacy in determining both Bos gaurus and Bos taurus lineage in the putative hybrids. The karyotyping results were confirmed by molecular analysis and our results provide a feasible way for detecting the putative hybrids between wild and domestic cattle in case of lacking the wild trace

Spatiotemporal evolution of SARS-CoV-2 Alpha and Delta variants during large nationwide outbreak of COVID-19, Vietnam, 2021

We analyzed 1,303 SARS-CoV-2 whole-genome sequences from Vietnam, and found the Alpha and Delta variants were responsible for a large nationwide outbreak of COVID-19 in 2021. The Delta variant was confined to the AY.57 lineage and caused >1.7 million infections and >32,000 deaths. Viral transmission was strongly affected by nonpharmaceutical interventions

Short Tandem Repeats Used in Preimplantation Genetic Testing of Î’-Thalassemia: Genetic Polymorphisms For 15 Linked Loci in the Vietnamese Population

BACKGROUND: β-thalassemia is one of the most common monogenic diseases worldwide. Preimplantation genetic testing (PGT) of β-thalassemia is performed to avoid affected pregnancies has become increasingly popular worldwide. In which, the indirect analysis using short tandem repeat (STRs) linking with HBB gene to detect different β-globin (HBB) gene mutation is a simple, accurate, economical and also provides additional control of contamination and allele-drop-out ADO. AIM: This study established microsatellite markers for PGT of Vietnamese β-thalassemia patient. METHODS: Fifteen (15) STRs gathered from 5 populations were identified by in silico tools within 1 Mb flanking the HBB gene. The multiplex PCR reaction was optimized and performed on 106 DNA samples from at-risk families. RESULTS: After estimating, PIC values were ≥ 0.7 for all markers, with expected heterozygosity and observed heterozygosity values ranged from 0.81 to 0.92 and 0.53 to 0.86, respectively. One hundred percent of individuals had at least seven heterozygous markers and were found to be heterozygous for at least two markers on either side of the HBB gene. The STRs panel was successfully performed on one at-risk family. CONCLUSION: In general, a pentadecaplex marker (all < 1 Mb from the HBB gene) assay was constituted for β-thalassemia PGT on Vietnamese population