Numerical simulation of free shear flows: Towards a predictive computational aeroacoustics capability

Implicit and explicit spatial differencing techniques with fourth order accuracy have been developed. The implicit technique is based on the Pade compact scheme. A Dispersion Relation Preserving concept has been incorporated into both of the numerical schemes. Two dimensional Euler computation of a spatially-developing free shear flow, with and without external excitation, has been performed to demonstrate the capability of numerical schemes developed. Results are in good agreement with theory and experimental observation regarding the growth rate of fluctuating velocity, the convective velocity, and the vortex-pairing process

Computational analysis of the flowfield of a two-dimensional ejector nozzle

A time-iterative full Navier-Stokes code, PARC, is used to analyze the flowfield of a two-dimensional ejector nozzle system. A parametric study was performed for two controlling parameters, duct to nozzle area ratio and nozzle pressure ratio. Results show that there is an optimum area ratio for the efficient pumping of secondary flow. At high area ratios, a freestream flow passes directly through the mixing duct without giving adequate pumping. At low area ratios, the jet boundary blocks the incoming flow. The nozzle pressure ratio variation shows that the pumping rate increases as the pressure ratio increases, provided there is no interaction between the shroud wall and the shock cell structure

Selective modulators of α5-containing GABAA receptors and their therapeutic significance

GABA receptors containing the α subunit (αGABARs) are found mainly in the hippocampus where they mediate a tonic chloride leak current and contribute a slow component to GABAergic inhibitory synaptic currents. Their inhibitory effect on the excitability of hippocampal neurons at least partly explains why changes in the level of activity of αGABARs affect cognition, learning and memory. These receptors have been implicated as potential therapeutic targets for a range of clinical conditions including age-related dementia, stroke, schizophrenia, Down syndrome and anesthetic- induced amnesia. Accordingly, a range of pharmacological modulators that selectively target αGABARs, as either inhibitors or allosteric enhancers, have been developed. Although many of these compounds show therapeutic effects in animal models of the above clinical disorders, none has been marketed yet due to unsuccessful clinical trials and toxicity in humans. These experiments have also revealed paradoxical effects of αGABAR modulation (e.g., cognitive impairments can be reversed by both positive and negative modulation), suggesting that our knowledge of the physiological roles of αGABARs is incomplete. This review highlights the various positive and negative modulators for αGABARs that have been developed, key findings concerning their effects in behavioral studies, and their importance across a number of therapeutic fields. It also highlights some of the gaps in our knowledge of the physiological and pathological roles of αGABARs

Numerical analyses for improved terminal velocity of deep water torpedo anchor

Purpose: This research aims to investigate the effects of manipulation of a torpedo’s geometries to attain higher terminal velocity. The parameters of interest include geometric changes of the original design, as well as sea water properties that reflect water depth in South China Sea. Design/methodology/approach: The research make use of computational fluid dynamics (CFD) software, FLUENT, to solve viscous incompressible Navier–Stokes equations with two equations k-epsilon turbulent model. The calculated drag coefficient is subsequently used to calculate the maximum attainable terminal velocity of the torpedo. Findings: It was found that the terminal velocity can be improved by sharper tip angle, greater aspect ratio, greater diameter ratio and optimum rear angle at 30°. Sensitivity of drag coefficient toward each of the parameters is established in this paper. Originality/value: The paper, in addition to verifying the importance of aspect ratio, has also established the tip angle, diameter ratio and rear angle of the torpedo as important geometric aspects that could be tuned to improve its terminal velocity

iBench: A ground truth approach for advanced validation of mass spectrometry identification method

The discovery of many noncanonical peptides detectable with sensitive mass spectrometry inside, outside, and on cells shepherded the development of novel methods for their identification, often not supported by a systematic benchmarking with other methods. We here propose iBench, a bioinformatic tool that can construct ground truth proteomics datasets and cognate databases, thereby generating a training court wherein methods, search engines, and proteomics strategies can be tested, and their performances estimated by the same tool. iBench can be coupled to the main database search engines, allows the selection of customized features of mass spectrometry spectra and peptides, provides standard benchmarking outputs, and is open source. The proof-of-concept application to tryptic proteome digestions, immunopeptidomes, and synthetic peptide libraries dissected the impact that noncanonical peptides could have on the identification of canonical peptides by Mascot search with rescoring via Percolator (Mascot+Percolator)

Knots modify the coil–stretch transition in linear DNA polymers

We perform single-molecule DNA experiments to investigate the relaxation dynamics of knotted polymers and examine the steady-state behavior of knotted polymers in elongational fields. The occurrence of a knot reduces the relaxation time of a molecule and leads to a shift in the molecule's coil-stretch transition to larger strain rates. We measure chain extension and extension fluctuations as a function of strain rate for unknotted and knotted molecules. The curves for knotted molecules can be collapsed onto the unknotted curves by defining an effective Weissenberg number based on the measured knotted relaxation time in the low extension regime, or a relaxation time based on Rouse/Zimm scaling theories in the high extension regime. Because a knot reduces a molecule's relaxation time, we observe that knot untying near the coil-stretch transition can result in dramatic changes in the molecule's conformation. For example, a knotted molecule at a given strain rate can experience a stretch-coil transition, followed by a coil-stretch transition, after the knot partially or fully unties.National Science Foundation (U.S.) (Grant CBET-1602406