Current Status of Poultry Meat Abnormalities

Over the past decade, the poultry industry has faced an increasing occurrence of growth-related muscular abnormalities that mainly affect fast-growing genotypes selected for their production performances (high growth rate and breast yield). These abnormalities, termed white striping (WS), wooden breast (WB) and spaghetti meat (SM), primarily affect the superficial portion of pectoralis major muscles. Despite their distinctive phenotypes, WS, WB, and SM conditions entail common histological features, i.e., they might share common causative mechanisms underpinning their occurrence. Meat affected by growth-related abnormalities is harmless for human nutrition since no specific biological or chemical hazards have been found to be related to its consumption. However, WS, WB, and SM abnormalities negatively affect both quality traits and technological properties of raw and processed meat, causing relevant economic damages in the poultry industry. This paper aims to provide an update about the current status of poultry meat abnormalities, giving useful insights about their impact on meat quality, the possible causative mechanisms, methods for mitigation, and future perspectives

Meat Quality for Further Processing

In the past few decades, the genetic selection programs carried out to increase the production traits of broilers and obtain high-growth rate and breast yield hybrids led to profound changes in muscle structure and metabolism resulting in an increased incidence of abnormalities mainly affecting the Pectoralis major muscle. White-striping (WS), wooden breast (WB) and poor cohesion (PC) defects are of relevant importance. Within this scenario, this PhD thesis aimed at deepening the current knowledge concerning histological features, quality traits and technological properties of broiler pectoral muscles affected by abnormalities, clarifying their peculiarities and similarities as well as investigating the underlying mechanisms involved in their occurrence. Irrespective of the type of abnormality, similar histological alterations including profound degenerative myopathic changes, variable fibers cross-sectional area as well as proliferation of loose connective tissue and fat deposition were observed within the endomysial and perimysial spaces of the Pectoralis major muscles affected by abnormalities. As for quality traits, the abnormal muscles exhibited severely impaired technological properties (reduced water-holding/-binding capacity and altered texture) with the alterations being more pronounced within the superficial layer of the cranial portion of the muscle when more than one abnormality coexist. With regard to chemical composition, an overall higher moisture, fat and collagen content to the detriment of protein and total heme pigments levels were found in abnormal muscles which exhibited altered sodium and calcium homeostasis as well as 204 differentially expressed genes related to several functional categories. In addition, the hardened consistency of the WB cases is only partially counteracted by the endogenous proteolytic processes taking place during the post-mortem period. In conclusion, it is reasonable to hypothesise the existence of a complex network of biological changes responsible for the phenotypic features and the consistent impairment of muscular metabolism of the abnormal muscles

Sarcomere lengths in wooden breast broiler chickens

The present study aims to measure the sarcomere lengths in normal broiler muscles and in non-lesion sites of breast muscles focally affected by Wooden Breast (WB). For this purpose, twenty Pectoralis major muscles (10 unaffected and 10 WB-focally affected cases) were sampled and used to measure sarcomere length by laser diffraction method. When compared with their unaffected counterpart, WB cases exhibited 13% longer sarcomeres (1.91 vs. 1.69 mu m; p <.001) measured within the non-lesioned site of the muscle. Although it is not simple to draw conclusions about the lesion properties based on the non-lesion area, but as the fibres are bound to each other, it may be reasonable to anticipate that the hardened consistency observed in WB is not ascribable to a more intense contraction of the sarcomeres. In addition, considering the current knowledge concerning this condition, it might be assumed that the longer sarcomeres observed in WB are not triggering the development of this condition but are rather a consequence of the profound alteration in the muscular structure resulting from it. Indeed, despite the outstanding improvements in the live and slaughtering traits, the selection programmes carried out in the past years have resulted in a reduced capillarization and impaired oxygen supply to the Pectoralis major of fast-growing hybrids thus affecting the physiology of its constituting fibres as well as maybe impairing their ability to synthetise new sarcomeres. This may result in a skeletal muscle injury, which would ultimately lead to necrosis and fibrosis.Peer reviewe

Spaghetti Meat Abnormality in Broilers: Current Understanding and Future Research Directions

open3noSpaghetti meat (SM) is a recent muscular abnormality that affects the Pectoralis major muscle of fast-growing broilers. As the appellative suggests, this condition phenotypically manifests as a loss of integrity of the breast muscle, which appears soft, mushy, and sparsely tight, resembling spaghetti pasta. The incidence of SM can reach up to 20% and its occurrence exerts detrimental effects on meat composition, nutritional value, and technological properties, accounting for an overall decreased meat value and important economic losses related to the necessity to downgrade affected meats. However, due to its recentness, the causative mechanisms are still partially unknown and less investigated compared to other muscular abnormalities (i.e., White Striping and Wooden Breast), for which cellular stress and hypoxia caused by muscle hypertrophy are believed to be the main triggering factors. Within this scenario, the present review aims at providing a clear and concise summary of the available knowledge concerning SM abnormality and concurrently presenting the existing research gaps, as well as the potential future developments in the field.openBaldi, Giulia; Soglia, Francesca; Petracci, MassimilianoBaldi, Giulia; Soglia, Francesca; Petracci, Massimilian

The role of histidine dipeptides on postmortem acidification of broiler muscles with different energy metabolism

It is generally held that the content of several free amino acids and dipeptides is closely related to the energy-supplying metabolism of skeletal muscles. Metabolic characteristics of muscles are involved in the variability of meat quality due to their ability to influence the patterns of energy metabolism not only in living animal but also during post-mortem time. Within this context, this study aimed at establishing whether the concentration of histidine dipeptides can affect muscle post-mortem metabolism, examining the glycolytic pathway of three chicken muscles (Pectoralis major, extensor iliotibialis lateralis and gastrocnemius internus as glycolytic, intermediate and oxidative-type, respectively) selected based on their histidine dipeptides content and ultimate pH. Thus, a total of 8 carcasses were obtained from the same flock of broiler chickens (Ross 308 strain, females, 49 days of age, 2.8 kg body weight at slaughter) and selected immediately after evisceration from the line of a commercial processing plant. Meat samples of about 1 cm3 were excised from bone-in muscles at 15, 60, 120 and 1,440 min post-mortem, instantly frozen in liquid nitrogen and used for the determination of pH, glycolytic metabolites, buffering capacity as well as histidine dipeptides content through 1H-NMR. Overall results suggest that glycolysis in leg muscles ceased already after 2 h post-mortem, while in breast muscle continued until 24 h, when it exhibited significantly lower pH values (P&lt;0.05). However, considering its remarkable glycolytic potential, Pectoralis major muscle should have exhibited a greater and faster acidification, suggesting that its higher (P&lt;0.05) histidine dipeptides\u2019 content might have prevented a potentially stronger acidification process. Accordingly, breast muscle also showed greater (P&lt;0.05) buffering ability in the pH range 6.0-7.0. Therefore, anserine and carnosine, being highly positively correlated with muscle\u2019s buffering capacity (P&lt;0.001), might play a role in regulating post-mortem pH decline, thus exerting an effect on muscle metabolism during pre-rigor phase and the quality of the forthcoming meat. Overall results also suggest that total histidine dipeptides content along with muscular ultimate pH represent good indicators for the energy-supplying metabolism of chicken muscles

Fiber Metabolism, Procollagen and Collagen Type III Immunoreactivity in Broiler Pectoralis Major Affected by Muscle Abnormalities

The present study aimed to evaluate the muscle fiber metabolism and assess the presence and distribution of both procollagen and collagen type III in pectoralis major muscles affected by white striping (WS), wooden breast (WB), and spaghetti meat (SM), as well as in those with macroscopically normal appearance (NORM). For this purpose, 20 pectoralis major muscles (five per group) were selected from the same flock of fast-growing broilers (Ross 308, males, 45-days-old, 3.0 kg live weight) and were used for histochemical (nicotinamide adenine dinucleotide tetrazolium reductase (NADH-TR) and alpha-glycerophosphate dehydrogenase (\u3b1-GPD)) and immunohistochemical (procollagen and collagen type III) analyses. When compared to NORM, we found an increased proportion (p &lt; 0.001) of fibers positively stained to NADH-TR in myopathic muscles along with a relevant decrease (p &lt; 0.001) in the percentage of those exhibiting a positive reaction to \u3b1-GPD. In addition, an increased proportion of fibers exhibiting a positive reaction to both stainings was observed in SM, in comparison with NORM (14.3 vs. 7.2%; p &lt; 0.001). After reacting to NADH-TR, SM exhibited the lowest (p &lt; 0.001) cross-sectional area (CSA) of the fibers ( 1212% with respect to NORM). On the other hand, after reacting to \u3b1-GPD, the CSA of WS was found to be significantly larger (+10%) in comparison with NORM (7480 vs. 6776 \ub5m2; p &lt; 0.05). A profound modification of the connective tissue architecture involving a different presence and distribution of procollagen and collagen type III was observed. Intriguingly, an altered metabolism and differences in the presence and distribution of procollagen and collagen type III were even observed in pectoralis major muscle classified as NORM

Molecular Pathways and Key Genes Associated With Breast Width and Protein Content in White Striping and Wooden Breast Chicken Pectoral Muscle

Growth-related abnormalities affecting modern chickens, known as White Striping (WS) and Wooden Breast (WB), have been deeply investigated in the last decade. Nevertheless, their precise etiology remains unclear. The present study aimed at providing new insights into the molecular mechanisms involved in their onset by identifying clusters of co-expressed genes (i.e., modules) and key loci associated with phenotypes highly related to the occurrence of these muscular disorders. The data obtained by a Weighted Gene Co-expression Network Analysis (WGCNA) were investigated to identify hub genes associated with the parameters breast width (W) and total crude protein content (PC) of Pectoralis major muscles (PM) previously harvested from 12 fast-growing broilers (6 normal vs. 6 affected by WS/WB). W and PC can be considered markers of the high breast yield of modern broilers and the impaired composition of abnormal fillets, respectively. Among the identified modules, the turquoise (r = -0.90, p &lt; 0.0001) and yellow2 (r = 0.91, p &lt; 0.0001) were those most significantly related to PC and W, and therefore respectively named “protein content” and “width” modules. Functional analysis of the width module evidenced genes involved in the ubiquitin-mediated proteolysis and inflammatory response. GTPase activator activity, PI3K-Akt signaling pathway, collagen catabolic process, and blood vessel development have been detected among the most significant functional categories of the protein content module. The most interconnected hub genes detected for the width module encode for proteins implicated in the adaptive responses to oxidative stress (i.e., THRAP3 and PRPF40A), and a member of the inhibitor of apoptosis family (i.e., BIRC2) involved in contrasting apoptotic events related to the endoplasmic reticulum (ER)-stress. The protein content module showed hub genes coding for different types of collagens (such as COL6A3 and COL5A2), along with MMP2 and SPARC, which are implicated inCollagen type IV catabolism and biosynthesis. Taken together, the present findings suggested that an ER stress condition may underly the inflammatory responses and apoptotic events taking place within affected PM muscles. Moreover, these results support the hypothesis of a role of the Collagen type IV in the cascade of events leading to the occurrence of WS/WB and identify novel actors probably involved in their onset

Technical note: Estimation of real rabbit meat consumption in Italy

[EN] As in other livestock species, the annual per capita consumption of rabbit meat is currently estimated as the ratio of the total weight of carcasses available for consumption to the number of inhabitants of a certain region. The aim of this work was to establish conversion coefficients from carcass to dible lean meat and estimate real rabbit meat consumption in Italy. Accordingly, a total of 24 rabbits were slaughtered at 2 different ages to obtain carcasses representative of the main market categories in Northern Italy: medium-size (carcass weight of about 1.4 kg) and heavy-size (carcass weight of about 1.8 kg). Chilled carcasses were used to determine offal, dissectible fat, bone and meat weights and yields. Experimentally obtained conversion factors from carcass to edible lean meat and estimated meat waste percentage at retail and consumption levels were subsequently used to estimate the real per capita amount of rabbit meat consumed in Italy. The finding of this study revealed that, if compared to the medium-size group, heavy-size carcasses had higher lean meat yield for both intermediate (92.9 vs. 92.4%; P<0.05) and hind parts (84.3 vs. 79.1%; P<0.001). On the contrary, the meat yield of fore part was higher in the medium-size group (66.2 vs. 65.5%; P<0.001) compared to heavy-size carcasses. Eventually, overall meat yield was higher in heavy-size carcasses compared to medium-size ones (64.4 vs. 63.2%; P<0.001). By using these conversion factors and estimated overall losses at retailing and home-consumption (15%), we estimated that real per capita annual rabbit meat consumption is 0.50 kg in Italy, which is only 54% compared to the estimated apparent consumption (0.90 kg).Petracci, M.; Soglia, F.; Baldi, G.; Balzani, L.; Mudalal, S.; Cavani, C. (2018). Technical note: Estimation of real rabbit meat consumption in Italy. World Rabbit Science. 26(1):91-96. doi:10.4995/wrs.2018.7802SWORD919626

Muscle Abnormalities and Meat Quality Consequences in Modern Turkey Hybrids

Turkey meat is the second most consumed poultry meat worldwide and represents an economic source of high-quality protein for human consumption. To fulfill the increasing demand for turkey meat, breeding companies have been selecting genetic lines with increased growth potential and breast muscle proportion. Moreover, the progressive shift toward further processed products has emphasized the need for higher standards in poultry meat to improve its technological characteristics and functional properties (i.e., water-holding capacity). However, as observed for broiler chickens, a growing body of scientific evidence suggests that the intense selection for the aforementioned traits could be associated with a greater occurrence of growth-related myopathies and abnormalities and, consequently, to increased downgrading rates and overall reduction of meat quality characteristics. In the past, muscle abnormalities such as deep pectoral myopathy, pale-soft-and-exudative-like meat, and focal myopathy have been reported in turkey lines selected for increased growth rate. In addition, the presence of white striations in the superficial layer of pectoralis major muscle, as well as the tendency of muscle fiber bundles to separate resulting in an altered breast muscle structure, has been detected in commercial turkey abattoirs. Furthermore, past investigations revealed the presence of another quality issue depicted by an overall toughening of the breast muscle. These meat abnormalities seem to macroscopically overlap the white striping, spaghetti meat, and wooden breast conditions observed in pectoral muscle of fast-growing, high-breast-yield chicken hybrids, respectively. Considering the high economic impact of these growth-related abnormalities in broilers, there is an increasing interest of the turkey industry in estimating the occurrence and the impact of these meat quality issues also in the modern turkey lines. Studies have been recently conducted to assess the effect of the genotype on the occurrence of these emerging growth-related defects and to evaluate how meat quality properties are affected by white-striping condition in turkeys, respectively. Therefore, this review aims to provide a critical overview of the current understanding regarding the growth-related abnormalities and their impact on meat quality in modern turkey hybrids with the hope that this information may improve the knowledge concerning their overall effect on poultry meat