Design of a simulator module for analysis of friction and wear of teeth during cleaning

Tato práce se zabývá návrhem modulu pro tribologický simulátor Bruker UMT TriboLab, který bude využit při výzkumu v oblasti analýz opotřebení tvrdých zubních tkání a výplňových materiálů v důsledku čištění zubů. Cílem této práce byl návrh podmínek experimentu, konstrukce modulu, tvorba výkresové dokumentace, výroba a následná realizace validačního experimentu. Podmínky experimentu byly stanoveny na základě rešerše a k vytvoření optimálního konstrukčního řešení byly průběžně vyráběny prototypy pomocí metody 3D tisku. Nakonec byly na základě výkresové dokumentace vyrobeny dva upínače zubního kartáčku a dva upínače vzorku zubní výplně. Následnou montáží upínačů a realizací experimentu na tribometru byla ověřena jejich funkčnost, čímž je možné prohlásit všechny cíle za úspěšně splněné. Nyní tyto upínače budou součástí několika výzkumných úkolů v oblasti orální biotribologie.This thesis focuses on the design of a module for the tribological simulator Bruker UMT TriboLab, which will be used in research in the field of analysis of wear of hard dental tissues and filling materials due to tooth cleaning. The aim of this work was the design of experimental conditions, module construction, creation of drawing documentation, production and subsequent implementation of a validation experiment. The conditions of the experiment were determined on the basis of research and to create an optimal design solution, prototypes were continuously produced using the 3D printing method. Finally, two toothbrush fixtures and two dental filling sample fixtures were made based on the drawing documentation. Subsequent assembly of the fixtures and the implementation of the experiment on the tribometer verified their functionality, which makes it possible to declare all the goals successfully met. Now these fixtures will be part of several research tasks in the field of oral biotribology.

Bioactive compounds evaluation in different types of Czech and Slovak honeys

Honey contains important bioactive compounds (enzymes, phenolic compounds, vitamins, and minerals) with several positive health effects for humans. In the study six types of honey (acacia, rape, floral, multi flower, forest, and honeydew honeys), of Czech and Slovak origin, were evaluated for bioactive compounds by means of color, polyphenols and antioxidant capacity analyses. The brightest color of honeys, the lowest values measured spectometrically, had acacia and rape honeys, followed by floral, and darker multi flower and forest honeys, and honeydew honeys. Polyphenols (PP) amount, determined by spectrophotometric method with Folin-Ciocalteu reagent, was highest for the darkest honeydew honeys, followed by multi flower and forest honey, brighter floral honeys, and rape and acacia honey. Honeys polyphenols were in the range from 54.0 to 254.2 mg GAE.100g -1 . The total antioxidant capacity (TAC) was analyzed by spectrometric methods with ABTS and DPPH reagents. Antioxidant capacity values are in agreement with the PP contents order. They were highest also for honeydew honeys (59.2 - 89.6 and 73.1 - 118.7 mg TE.100g -1 ), followed by multi flower (66.0 and 56.7 mg TE.100g -1 ) and forest honey (56.0 and 49.1 mg TE.100g -1 ), then floral honeys (33.0 - 49.2 and 27.8 - 38.7 mg TE.100g -1 ) and the lowest values for rape (19.0 and 28.1 mg TE.100g -1 ) and acacia (15.5 and 11.3 mg TE.100g -1 ) honey. A positive correlation between color, PP amount and TAC was evaluated for analyzed honeys. Darker honey samples showed higher values of phenolic compounds and antioxidant potential, therefore they belong to the honey types with higher amount of bioactive compounds such as antioxidants. © 2019 Potravinarstvo Slovak Journal of Food Sciences

Bioactive compounds, antioxidant activity, and biological effects of european cranberry (vaccinium oxycoccos)

Lesser known fruits or underutilized fruit species are recently of great research interest due to the presence of phytochemicals that manifest many biological effects. European cranberry, Vaccinium oxycoccos fruit, as an important representative of this group, is a valuable source of antioxidants and other biologically active substances, similar to American cranberry (V. macrocarpon) which is well known and studied. European cranberry fruit is rich especially in polyphenolic compounds anthocyanins (12.4⁻207.3 mg/100 g fw), proanthocyanins (1.5⁻5.3 mg/100 g fw), and flavonols, especially quercetin (0.52⁻15.4 mg/100 g fw), which mostly contribute to the antioxidant activity of the fruit. Small cranberry is also important due to its various biological effects such as urinary tract protection (proanthocyanidins), antibacterial and antifungal properties (quercetin, proanthocyanidins, anthocyanins), cardioprotective (proanthocyanidins) and anticancer activities (proanthocyanidins), and utilization in food (juice drinks, jams, jellies, sauces, additive to meat products) and pharmacological industries, and in folk medicine.internal grant agency of Tomas Bata University in Zlin [IGA/FT/2018/006, VEGA 1/0083/16

Natural fruit beverages fortified by biologically active substances of grape vines

Based on the study of general knowledge of biochemical and all subsequent developmental studies of organic matter, especially products of grapevine and selected fruit products, a comprehensive study of processing technologies is prepared. Use of a combination of vine products and fruit products in the form of natural grapes. Beverages are researched and developed to be purely natural on the basis of grape musts, blue and white, either individually and again separately in targeted combinations, both biochemically, organoleptically and colorfully, with fruit sources. The core of grape value of biologically active substances is an integral and essential new part and condition of designing these beverages. Their increased biological values, which create the preconditions for containment and if properly managed on the basis of scientific knowledge, may in some cases almost result in the elimination of synthetic additives. It should be noted that 20 - 25% of the adult population suffers from many unexpected allergies, for example, to the sulphite content, although its content in the final product does not exceed the health-approved normatives. And there are many other, interrelated relationships. Beverages are technologically dealt with both without alcohol fermentation and with this fermentation, but only based on their compositional natural resources. They are therefore suitable for the entire population profile. The whole set contains 7 variants and a combination of natural beverages from different fruits. Including natural beverages with or without alcoholic fermentation from the must of white wine grapes, the juice of apple puree with those of biologically active substances from the products grapevine. Three months of monitoring and determination of basic (oenological) values and biologically active substances were performed on these products. The high-performance liquid chromatography method with a refractometric detector determined amount of sugar and alcohol, whilst titrating determined total and volatile acids and free sulfur dioxide. Yeast assimilable nitrogen, total anthocyanins and polyphenols were determined by spectrophotometry, antioxidant activity by DPPH and ABTS methods. © 2019 Potravinarstvo Slovak Journal of Food Sciences

Polyphenols and antioxidant capacity in different types of garlic

Garlic contains important biologically active compounds such as phytoncides, antioxidants and others. There belong organosulfur compounds, allyl thiosulfinates and phenolic compounds (phenolic acids, flavonoids), vitamins (E and C) and some minerals, that all have several positive effects on human health. In the work five types of raw garlic (Allium sativum) of Czech, Chinese and Spanish origin, and bear's garlic (Allium ursinum), and two dried garlic products, were evaluated for polyphenols content and antioxidant capacity. The highest values of total polyphenols (TP), analyzed by spectrometric method with Folin-Ciocalteu reagent, had fresh samples of bear's and Spanish garlic; the lowest ones were evaluated for Czech garlic bulbs (92.2-119.6 mg GAE.100g-1 fw) and dry garlic products (70.1-84.5 mg GAE.100g-1). The total antioxidant capacity (TAC) was determined by spectrometric methods with DPPH and ABTS reagents. To types of fresh garlic with the best values of antioxidant capacity, evaluated by both methods, belong bear's and Spanish garlic, followed by Chinese and Czech garlic samples. These values are in agreement with polyphenols content in garlic bulbs. Dry garlic products had the highest values of TAC. Content of polyphenols and antioxidant capacity values were positively correlated, higher correlation value was detected for TP and TAC-ABTS (0.973) than for TP and TAC-DPPH (0.873). Bear's garlic and garlic belong to the vegetable types with high amount of biologically active compounds such as antioxidants. © 2018 Potravinarstvo Slovak Journal of Food Sciences

Comparative study on natural plant antibiotics - Vegetable and their consumption among college students

The research study is aimed at evaluation of natural plant antibiotics utilization among college students (554) with different subject study (Pre-school and elementary education, Biology, Regional Tourism, Horticulture, Physical education) from 3 countries - Slovak Republic, Czech Republic and Hungary. The attention has been focused on natural antibiotics in plants - vegetables (garlic, onion and horse radish) and the frequency of their consumption among college students. From the research results there is evident that majority of students had basic knowledge about natural plant antibiotics (85% of respondents) and they utilize them in everyday life (60.3%). The prevailing number of students utilizes synthetic antibiotics only rarely - once a year (33.4%) or never (37.5%). From achieved results about exact plants (garlic, onion and horse radish) consumption, the majority of respondents consume garlic once a week (42.2%); on the daily base the highest usage was noticed in the group of Slovak students with the subject of Physical education (32.1%) that could be considered as statistically different in comparison with the rest of groups. On the contrary, the lowest garlic consumption was noticed for students of biology (23.5%) and only small amount of students (3.6%) claimed that they have never included garlic into their diet. As for the onion, the majority of respondents (42.10%) also consume this commodity once a week; everyday consumption was noticed again especially between Slovak students with the subject of Physical education (32.1%) and Horticulture (31.1%). The results of these groups significantly differed from results of other groups. Third studied vegetable, horse-radish, it has never been consumed by Slovak students of Pre-school and elementary education in Slovak language (47.9%) that has been significantly distinguishable from another groups. Also Hungarian students of Physical education consume this commodity rarely (30.6%) - only once a year. Major part of students (46%) consumes horse-radish once a month. Czech students of Horticulture prefer consummation of horse-radish only once a month (76.8%). The lowest utilization was noticed in groups MU/ H (76.8%) consumed this commodity only monthly. Similarly, students of UP/ PE (30.6%) consumed horse radish only once a year that was significantly lower value in comparison with the rest of evaluated groups. © 2017 Potravinarstvo Slovak Journal of Food Sciences

Monitoring of bioactive compounds of tomato cultivars as affected by mulching film

The influence of varieties and the effect of mulching film on antioxidant capacity, polyphenol content, ascorbic acid content, and yield of tomato fruits were investigated. Results of two years (2012 and 2013) investigations were compared. The results proved a statistically significant effect of year, mulching film, and variety on the content of total polyphenols (0.92-1.49 g gallic acid equivalents per kg of fresh weight (FW), ascorbic acid (26.66-38.62 mg per 100 g FW), and antioxidant capacity (1.12-1.94 g ascorbic acid equivalents per kg FW), while the values were the highest in 2013 and in uncovered soil. Conversely, a higher yield was found in mulching film compared with uncovered soil, also in 2013 (48.65-120.38 t ha-1). There was a negative correlation between the yield and the content of bioactive compounds (BC) and antioxidant capacity. The content of bioactive substances, antioxidant capacity, and yield of tomato fruits is dependent on the vintage, agronomical interventions, and genotype. © 2018 M. Valšíková et al., published by Sciendo 2018

Fruits of black chokeberry aronia melanocarpa in the prevention of chronic diseases

In recent years, growing attention has been focused on the utilization of natural sources of antioxidants in the prevention of chronic diseases. Black chokeberry (Aronia melanocarpa) represents a lesser known fruit species utilized mainly as juices, purees, jams, jellies and wine, as important food colorants or nutritional supplements. The fruit is valued as a great source of antioxidants, especially polyphenols, such as phenolic acids (neochlorogenic and chlorogenic acids) and flavonoids (anthocyanins, proanthocyanidins, flavanols and flavonols), particularly cyanidin-3-galactoside and cyanidin-3-arabinoside, as well as (-)-epicatechin units. The berries of A. melanocarpa, due to the presence and the high content of these bioactive components, exhibit a wide range of positive effects, such as strong antioxidant activity and potential medicinal and therapeutic benefits (gastroprotective, hepatoprotective, antiproliferative or anti-inflammatory activities). They could be also contributory toward the prevention of chronic diseases including metabolic disorders, diabetes and cardiovascular diseases, because of supportive impacts on lipid profiles, fasting plasma glucose and blood pressure levels. © 2017 by the authors.internal grant agency of Tomas Bata University in Zlin [IGA /FT/2017/006

Solving power outages in healthcare facilities: Algorithmisation and assessment of preparedness

Power outages present a significant threat to all of humanity. However, elements of critical infrastructure are most affected by this event. Among other things, healthcare facilities can be considered critical infrastructure. They are the backbone for saving lives. However, even these medical facilities can be endangered. The primary aim of this paper is to design an algorithm to assess healthcare facilities to solve power outages. Several inputs are considered for the evaluation of healthcare facilities. Based on these inputs, we could gain several outputs, such as object priority, preparedness of the healthcare facility, missing aggregate capacity and fuel stocks. Calculations to obtain this information are available in this article. This algorithm follows up the methodology for the categorisation and prioritisation of objects necessary for the resumption of electricity supply after a blackout. We conclude that only 15% of the surveyed healthcare facilities are prepared for a power outage.Univerzita Tomáše Bati ve Zlíněproject Tomas Bata University in Zlin, DKRVO/FLK