Fluctuating Nonlinear Spring Model of Mechanical Deformation of Biological Particles

We present a new theory for modeling forced indentation spectral lineshapes of biological particles, which considers non-linear Hertzian deformation due to an indenter-particle physical contact and bending deformations of curved beams modeling the particle structure. The bending of beams beyond the critical point triggers the particle dynamic transition to the collapsed state, an extreme event leading to the catastrophic force drop as observed in the force (F)-deformation (X) spectra. The theory interprets fine features of the spectra: the slope of the FX curves and the position of force-peak signal, in terms of mechanical characteristics --- the Young's moduli for Hertzian and bending deformations E_H and E_b, and the probability distribution of the maximum strength with the strength of the strongest beam F_b^* and the beams' failure rate m. The theory is applied to successfully characterize the $FX$ curves for spherical virus particles --- CCMV, TrV, and AdV

З листування Л. Биковського з Є. Бачинським

У статті надається листування видатного бібліографа, історика Л. Биковського з визначним українським громадським і церковним діячем Є. Бачинським. листи написані в 1947–1952 рр.В статье представлена переписка выдающегося библиографа, историка Л. Быковского с выдающимся украинским общественным и церковным деятелем Е. Бачинским. Письма написаны в 1947-1952 гг.The article consists of correspondence of the outstanding bibliographer, historian L.Bykovskyi with the outstanding Ukrainian public and church figure Ye. Bachynskyi. Letters are written in 1947–1952

Equine Arteritis Virus Subgenomic RNA Transcription: UV Inactivation and Translation Inhibition Studies

AbstractThe expression of the genetic information of equine arteritis virus (EAV), an arterivirus, involves the synthesis of six subgenomic (sg) mRNAs. These are 5′ and 3′ coterminal since they are composed of a leader and a body sequence, which are identical to the 5′ and 3′ ends of the genome, respectively. Previously, it has been suggested thatcis-splicing of a genome-length precursor RNA is involved in their synthesis. This was reevaluated in a comparative analysis of the sg RNA synthesis of EAV, the coronavirus mouse hepatitis virus (MHV), and the alphavirus Sindbis virus. UV transcription mapping showed that the majority of the EAV sg RNAs made at later stages of infection is not derived from a genome-length precursor. However, complete independence of sg RNA synthesis from that of genomic RNA was never observed during the course of infection. The possibility that this resulted from UV irradiation-induced effects on the synthesis of the viral replicase was investigated by inhibiting translation using cycloheximide. For EAV, ongoing protein synthesis was found to be more important for the synthesis of sg RNA than for that of genomic RNA. In general, MHV transcription was extremely sensitive to translation inhibition, whereas EAV genomic RNA synthesis became independent ofde novoprotein synthesis late in infection

Partner choice and cooperation in social dilemmas can increase resource inequality

Cooperation is more likely when individuals can choose their interaction partner. However, partner choice may be detrimental in unequal societies, in which individuals differ in available resources and productivity, and thus in their attractiveness as interaction partners. Here we experimentally examine this conjecture in a repeated public goods game. Individuals (n = 336), participating in groups of eight participants, are assigned a high or low endowment and a high or low productivity factor (the value that their cooperation generates), creating four unique participant types. On each round, individuals are either assigned a partner (assigned partner condition) or paired based on their self-indicated preference for a partner type (partner choice condition). Results show that under partner choice, individuals who were assigned a high endowment and high productivity almost exclusively interact with each other, forcing other individuals into less valuable pairs. Consequently, pre-existing resource differences between individuals increase. These findings show how partner choice in social dilemmas can amplify resource inequality

Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production

The arterivirus family (order Nidovirales) of single-stranded, positive-sense RNA viruses includes porcine respiratory and reproductive syndrome virus and equine arteritis virus (EAV). Their replicative enzymes are translated from their genomic RNA, while their seven structural proteins are encoded by a set of small, partially overlapping genes in the genomic 3′-proximal region. The latter are expressed via synthesis of a set of subgenomic mRNAs that, in general, are functionally monocistronic (except for a bicistronic mRNA encoding the E and GP2 proteins). ORF5, which encodes the major glycoprotein GP5, has been used extensively for phylogenetic analyses. However, an in-depth computational analysis now reveals the arterivirus-wide conservation of an additional AUG-initiated ORF, here termed ORF5a, that overlaps the 5′ end of ORF5. The pattern of substitutions across sequence alignments indicated that ORF5a is subject to functional constraints at the amino acid level, while an analysis of substitutions at synonymous sites in ORF5 revealed a greatly reduced frequency of substitution in the portion of ORF5 that is overlapped by ORF5a. The 43–64 aa ORF5a protein and GP5 are probably expressed from the same subgenomic mRNA, via a translation initiation mechanism involving leaky ribosomal scanning. Inactivation of ORF5a expression by reverse genetics yielded a severely crippled EAV mutant, which displayed lower titres and a tiny plaque phenotype. These defects, which could be partially complemented in ORF5a-expressing cells, indicate that the novel protein, which may be the eighth structural protein of arteriviruses, is expressed and important for arterivirus infection

Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production

The arterivirus family (order Nidovirales) of single-stranded, positive-sense RNA viruses includes porcine respiratory and reproductive syndrome virus and equine arteritis virus (EAV). Their replicative enzymes are translated from their genomic RNA, while their seven structural proteins are encoded by a set of small, partially overlapping genes in the genomic 3′-proximal region. The latter are expressed via synthesis of a set of subgenomic mRNAs that, in general, are functionally monocistronic (except for a bicistronic mRNA encoding the E and GP2 proteins). ORF5, which encodes the major glycoprotein GP5, has been used extensively for phylogenetic analyses. However, an in-depth computational analysis now reveals the arterivirus-wide conservation of an additional AUG-initiated ORF, here termed ORF5a, that overlaps the 5′ end of ORF5. The pattern of substitutions across sequence alignments indicated that ORF5a is subject to functional constraints at the amino acid level, while an analysis of substitutions at synonymous sites in ORF5 revealed a greatly reduced frequency of substitution in the portion of ORF5 that is overlapped by ORF5a. The 43–64 aa ORF5a protein and GP5 are probably expressed from the same subgenomic mRNA, via a translation initiation mechanism involving leaky ribosomal scanning. Inactivation of ORF5a expression by reverse genetics yielded a severely crippled EAV mutant, which displayed lower titres and a tiny plaque phenotype. These defects, which could be partially complemented in ORF5a-expressing cells, indicate that the novel protein, which may be the eighth structural protein of arteriviruses, is expressed and important for arterivirus infection

Experimental Research on the Behaviour of Combined Web Crippling and Bending of Steel Deck Sections

At an interior support, sections of cold-formed steel are subjected to a concentrated load and a bending moment Existing design rules describing the section failure at an interior support are subject to improvement and are not based on the section\u27s physical failure behaviour. In the last decade, several analytical models have been developed that predict the section ultimate concentrated load and directly include the influence of the bending moment, so that an empirical interaction method is not needed. However, the authors believe that these models are correct only for a concentrated load and a small bending moment In practice large bending moments occur. Therefore, the aim of the current research project is to develop an analytical model for trapezoidal hat sections subjected to a concentrated load and a bending moment as occurring in practice. The development of this model will be based on both experimental and numerical research will be carried out. In this article, a part of the experimental research will be presented

Experimental Research on the Behaviour of Combined Web Crippling and Bending of Steel Deck Sections

At an interior support, sections of cold-formed steel are subjected to a concentrated load and a bending moment Existing design rules describing the section failure at an interior support are subject to improvement and are not based on the section\u27s physical failure behaviour. In the last decade, several analytical models have been developed that predict the section ultimate concentrated load and directly include the influence of the bending moment, so that an empirical interaction method is not needed. However, the authors believe that these models are correct only for a concentrated load and a small bending moment In practice large bending moments occur. Therefore, the aim of the current research project is to develop an analytical model for trapezoidal hat sections subjected to a concentrated load and a bending moment as occurring in practice. The development of this model will be based on both experimental and numerical research will be carried out. In this article, a part of the experimental research will be presented

TRAINING YOUNG HASS AVOCADO TREES INTO A CENTRAL LEADER FOR ACCOMMODATION IN HIGHER DENSITY ORCHARDS

Abstract Avocado trees should be shaped in the nursery and trained immediately after planting to a central leader. It is necessary to balance the side shoot growth at specific times and remove strong upright water shoots. The resultant conical or pyramidal tree shape will allow better light interception by the leaf canopy and improved light penetration into the canopy for maintaining bearing wood and avoiding unproductive bare areas. Small timeous manipulations will make later major pruning, which could stimulate deleterious vegetative growth, unnecessary. Early attention to tree shape will make higher density plantings manageable and highly efficient trees a reality