Carbon Storage Potential and Carbon Dioxide Emissions from Mineral-Fertilized and Manured Soil

Two important goals of sustainable agriculture are food production and preserving and improving soil health. The soil organic carbon content is considered an indicator of soil health. The evaluation of the methods to increase the soil organic carbon content in long-term experiments is usually carried out without considering its environmental effects, (e.g., CO2–C soil emission). This study hypothesized that sandy soils have a low carbon storage potential, and that the carbon accumulation in the soil is accompanied by increased CO2–C emissions into the atmosphere. The study was carried out as a long-term fertilization experiment in Central Poland using a rye monoculture. The changes in the soil organic carbon content (SOC), CO2–C emissions from soil, and plant yields were examined for two soil treatments: one treated only with mineral fertilizers (CaNPK) and one annually fertilized with manure (Ca + M). Over the 91 years of the experiment, the SOC content of the manure-fertilized treatment increased almost two-fold, reaching 10.625 g C kg−1 in the topsoil, while the content of the SOC in the soil fertilized with CaNPK did not change (5.685 g C kg−1 in the topsoil). Unlike mineral fertilization, soil manuring reduced the plant yields by approximately 15.5–28.3% and increased the CO2–C emissions from arable land. The CO2–C emissions of the manured soil (5365.0 and 5159.2 kg CO2–C ha−1 in the first and second year of the study, respectively) were significantly higher (by 1431.9–2174.2 kg CO2–C ha−1) than those in the soils that only received mineral fertilizers (3933.1 and 2975.0 kg CO2–C ha−1 in the first and second year of the study, respectively). The results from this experiment suggest that only long-term fertilization with manure might increase the carbon storage in the sandy soil, but it is also associated with higher CO2–C emissions into the atmosphere. The replacement of mineral fertilizers with manure, predicted as a result of rising mineral fertilizer prices, will make it challenging to achieve the ambitious European goal of carbon neutrality in agriculture. The increase in CO2–C emissions due to manure fertilization of loamy sand soil in Central Poland also suggests the need to research the emissivity of organic farming

Genetic and Functional Diversity of Bacterial Microbiome in Soils With Long Term Impacts of Petroleum Hydrocarbons

Soil contamination with petroleum, especially in the area of oil wells, is a serious environmental problem. Restoring soil subjected to long-term pollution to its original state is very difficult. Under such conditions, unique bacterial communities develop in the soil that are adapted to the contaminated conditions. Analysis of the structure and function of these microorganisms can be a source of valuable information with regard to bioremediation. The aim of this study was to evaluate structural and functional diversity of the bacterial communities in soils with long-term impacts from petroleum. Samples were taken from the three oldest oil wells at the Crude Oil Mine site in Węglówka, Poland; the oldest was established in 1888. They were collected at 2 distances: (1) within a radius of 0.5 m from the oil wells, representing soil strongly contaminated with petroleum; and (2) 3 m from the oil wells as the controls. The samples were analyzed by 16S rRNA sequencing and the community level physiological profiling (CLPP) method in order to better understand both the genetic and functional structure of soil collected from under oil wells. Significant differences were found in the soil samples with regard to bacterial communities. The soils taken within 0.5 m of the oil wells were characterized by the highest biodiversity indexes. Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria were strongly correlated with biological activity in these soils. Families of Alphaproteobacteria were also dominant, including: Bradyrhizobiaceae, Rhizobiaceae, Rhodobacteraceae, Acetobacteraceae, Hyphomicrobiaceae, and Sphingomonadaceae. The study showed that the long term contamination of soil changes bacterial communities and their metabolic activity. Even so, natural bioremediation leads to the formation of specific groups of bacteria that actively grow at the site of contamination in the soil

Cryptic SARS-CoV-2 lineage identified on two mink farms as a possible result of long-term undetected circulation in an unknown animal reservoir, Poland, November 2022 to January 2023

In late 2022 and early 2023, SARS-CoV-2 infections were detected on three mink farms in Poland situated within a few km from each other. Whole-genome sequencing of the viruses on two of the farms showed that they were related to a virus identified in humans in the same region 2 years before (B.1.1.307 lineage). Many mutations were found, including in the S protein typical of adaptations to the mink host. The origin of the virus remains to be determined.</p

Inhibition of caspase-1 prolongs survival of mice infected with rabies virus

Rabies virus infects almost all mammals resulting in lethal disease. To date there is no treatment available for symptomatic rabies and there is an urgent need to develop treatment strategies that would prolong survival, thereby providing a window of opportunity for the host to mount a protective immune response. We hypothesized that both virus and excessive immune response contribute to disease and that interfering with both is necessary to prevent lethal disease. Here, we have inhibited the pro-inflammatory response associated with pyroptosis and showed that inhibition of CASP-1 had a beneficial effect on survival time. Our results confirm that some inflammatory responses may be involved in the pathogenesis of severe disease and the results suggest that effective intervention includes inhibition of virus and host response

Hantavirus Brno loanvirus is highly specific to the common noctule bat (Nyctalus noctula) and widespread in Central Europe.

Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations

Polish Soil Classification, 6th edition – principles, classification scheme and correlations

The sixth edition of the Polish Soil Classification (SGP6) aims to maintain soil classification in Poland as a modern scientific system that reflects current scientific knowledge, understanding of soil functions and the practical requirements of society. SGP6 continues the tradition of previous editions elaborated upon by the Soil Science Society of Poland in consistent application of quantitatively characterized diagnostic horizons, properties and materials; however, clearly referring to soil genesis. The present need to involve and name the soils created or naturally developed under increasing human impact has led to modernization of the soil definition. Thus, in SGP6, soil is defined as the surface part of the lithosphere or the accumulation of mineral and organic materials permanently connected to the lithosphere (through buildings or permanent constructions), coming from weathering or accumulation processes, originated naturally or anthropogenically, subject to transformation under the influence of soilforming factors, and able to supply living organisms with water and nutrients. SGP6 distinguishes three hierarchical categories: soil order (nine in total), soil type (basic classification unit; 30 in total) and soil subtype (183 units derived from 62 unique definitions; listed hierarchically, separately in each soil type), supplemented by three non-hierarchical categories: soil variety (additional pedogenic or lithogenic features), soil genus (lithology/parent material) and soil species (soil texture). Non-hierarchical units have universal definitions that allow their application in various orders/types, if all defined requirements are met. The paper explains the principles, classification scheme and rules of SGP6, including the key to soil orders and types, explaining the relationships between diagnostic horizons, materials and properties distinguished in SGP6 and in the recent edition of WRB system as well as discussing the correlation of classification units between SGP6, WRB and Soil Taxonomy