Vulnerability and Adaptability of Mangrove Forests on Misali Island, Zanzibar, Tanzania

As climate change threatens to cause heightened sea levels and decreased rainfall patterns in the Indian Ocean, mangrove forests are forced to adapt or suffer. This study aims to analyze the persistence of four prominent mangrove forests on Misali Island based on vulnerability and adaptability. The study focuses on three types of forests: overwash, coastal fringing, and scrub. Forest A, an overwash forest, was the main site of study and was surveyed for species type and seedling/sapling counts using ten transects which ranged from 40 to 91.7 meters in length. Data was analyzed in order to determine biodiversity, zonation, and reproductive success of the forest as a whole. Each factor was ranked on a scale of vulnerability to adaptability based on corresponding numerical values. Results showed the presence of seven species in Forest A with a high biodiversity. The forest was split into four zones which displayed strong zonation, as certain species prefer more inundated niches while others prefer drier niches. Overwash forests were found to be more adaptable than both coastal fringing and scrub forests and will likely persist the longest on Misali Island

The Effects of COVID-19-Related Stressors on Bruxism and Temporomandibular Disorders

Problem: COVID-19 has proven to be a major public health emergency, with an array of physical and emotional complications. While COVID-19 has its own effects on the human body, anxiety induced by the pandemic has shown to take a toll on the human oral cavity by means of causing individuals to grind and clench their teeth due to anxiety/stress, potentially leading to temporomandibular disorders and orofacial pain. Methods: A thorough review of literature was conducted by consulting credible studies published on online databases such as PubMed, Dentistry & Oral Sciences Source, Google Scholar, and Academic Search Complete. The studies examined were all conducted within the last five years (2016 and newer). Using key terms and phrases, a series of primary and secondary sources were analyzed in order to determine the effect of COVID-19 on bruxism and orofacial pain/temporomandibular disorders. Major findings: Researchers have found a direct correlation between the increase of anxiety, stress, and fear during the COVID-19 pandemic and the increase in bruxism and facial pain. Conclusions: In conclusion, the COVID-19 pandemic may result in the accumulation of emotional stress, which may have a profound impact on many aspects of health, including oral health. The studies have found that psychological factors such as stress and anxiety, associated with the Covid-19 pandemic, may lead to a greater risk of developing or worsening bruxism and temporomandibular joint disorders, which in turn would lead to increased orofacial pain. It has been encouraged that dental staff should pay more attention to the spread of such orofacial disorders during the pandemic. They should become more acquainted with the diagnostic methods of such disorders, their treatment options, and techniques for reducing stress to reduce the spread of such oral complications.https://scholarscompass.vcu.edu/denh_student/1029/thumbnail.jp

Students\u27 use of personal technology in the classroom: analyzing the perceptions of the digital generation

Faculty frequently express concerns about students’ personal use of information and communication technologies in today’s university classrooms. As a requirement of a graduate research methodology course in a university in Ontario, Canada, the authors conducted qualitative research to gain an in-depth understanding of students’ perceptions of this issue. Their findings reveal students’ complex considerations about the acceptability of technology use. Their analysis of the broader contexts of students’ use reveals that despite a technological revolution, university teaching practices have remained largely the same, resulting in ‘cultural lag’ within the classroom. While faculty are technically ‘in charge’, students wield power through course evaluations, surveillance technologies and Internet postings. Neoliberalism and the corporatisation of the university have engendered an ‘entrepreneurial student’ customer who sees education as a means to a career. Understanding students’ perceptions and their technological, social and political contexts offers insights into the tensions within today’s classrooms

Case Report on Leiomyosarcoma of the Vulva: A Rare Pathology

Leiomyosarcoma (LMS) of the vulva is rare. However it is the most common histologic subtype of vulvar sarcoma, accounting for approximately 1% of all vulvar malignancies. [1-8] Whether genetics and epigenetics play a role in pathogenesis is unclear. [1] The tumor is slow-growing with non-specific symptoms, has high metastatic potential, and follows a bimodal age distribution. [1-8] Diagnosis and prognosis are based upon immunohistochemical expression and criteria from early literature. [1,3,5-7] The most common therapeutic approach involves radical vulvectomy with lymph node resection. The value of adjuvant chemotherapy and radiation remains unknown. [1,3,5-8] Our case describes a 46-year-old Caucasian G2P2 female with LMS of the left labia

Inhibition of IκB Kinase by Vaccinia Virus Virulence Factor B14

The IκB kinase (IKK) complex is a key regulator of signal transduction pathways leading to the induction of NF-κB-dependent gene expression and production of pro-inflammatory cytokines. It therefore represents a major target for the development of anti-inflammatory therapeutic drugs and may be targeted by pathogens seeking to diminish the host response to infection. Previously, the vaccinia virus (VACV) strain Western Reserve B14 protein was characterised as an intracellular virulence factor that alters the inflammatory response to infection by an unknown mechanism. Here we demonstrate that ectopic expression of B14 inhibited NF-κB activation in response to TNFα, IL-1β, poly(I:C), and PMA. In cells infected with VACV lacking gene B14R (vΔB14) there was a higher level of phosphorylated IκBα but a similar level of IκBα compared to cells infected with control viruses expressing B14, suggesting B14 affects IKK activity. Direct evidence for this was obtained by showing that B14 co-purified and co-precipitated with the endogenous IKK complex from human and mouse cells and inhibited IKK complex enzymatic activity. Notably, the interaction between B14 and the IKK complex required IKKβ but not IKKα, suggesting the interaction occurs via IKKβ. B14 inhibited NF-κB activation induced by overexpression of IKKα, IKKβ, and a constitutively active mutant of IKKα, S176/180E, but did not inhibit a comparable mutant of IKKβ, S177/181E. This suggested that phosphorylation of these serine residues in the activation loop of IKKβ is targeted by B14, and this was confirmed using Ab specific for phospho-IKKβ

Intra-dance variation among waggle runs and the design of efficient protocols for honey bee dance decoding

Noise is universal in information transfer. In animal communication, this presents a challenge not only for intended signal receivers, but also to biologists studying the system. In honey bees, a forager communicates to nestmates the location of an important resource via the waggle dance. This vibrational signal is composed of repeating units (waggle runs) that are then averaged by nestmates to derive a single vector. Manual dance decoding is a powerful tool for studying bee foraging ecology, although the process is time-consuming: a forager may repeat the waggle run 1- >100 times within a dance. It is impractical to decode all of these to obtain the vector; however, intra-dance waggle runs vary, so it is important to decode enough to obtain a good average. Here we examine the variation among waggle runs made by foraging bees to devise a method of dance decoding. The first and last waggle runs within a dance are significantly more variable than the middle run. There was no trend in variation for the middle waggle runs. We recommend that any four consecutive waggle runs, not including the first and last runs, may be decoded, and we show that this methodology is suitable by demonstrating the goodness-of-fit between the decoded vectors from our subsamples with the vectors from the entire dances

“You are not alone”: A big data and qualitative analysis of men's unintended fatherhood

Background: Becoming a father is a profound change in a man's life that is not always planned or wanted. Little is known about the subjective experiences of men who become fathers unintentionally or reluctantly. The aim of this research was to explore how men who did not intend to have children discuss their feelings about becoming a father in an online, anonymous environment. We sought insights into emotional responses, appraisals of family functioning, and relationships with infants. Method: Data were collected from two Reddit forums for new and expectant fathers, r/Daddit and r/Predaddit. Approximately 2600 posts and 21,000 comments were extracted from the period between January 2019 and March 2020. We employed a two-stage methodology, blending big data techniques and qualitative analyses. Stage One included extraction and data preparation for topic modelling Stage Two was an adapted approach to thematic qualitative analysis. Results: Topic modelling revealed 49 topics of which 6 were relevant thematically to unintended fatherhood. Men's communication in these were then classified within three domains: 1) Men's Concerns included their mental health, problems bonding with baby, their relationships with family and partner, and finances; 2) Men's Affective Experiences existed on a spectrum of complex emotions including regret, resignation, ambivalence, acceptance, and excitement; and 3) the Purpose of Communication included asking for and offering advice, normalisation, and perspective. Conclusions: Online forums like Reddit provide a unique opportunity for fathers who did not intend to have children to normalize their experience by expressing concerns and emotions in a pseudonymous environment. This study highlights the supportive environment that online discussions offer to fathers, and particularly unexpected fathers who may face stigma or barriers in other settings

Lipidomic profiling of plasma free fatty acids in type-1 diabetes highlights specific changes in lipid metabolism

This research was funded by the British Heart Foundation [grant numbers PG/15/9/31270, FS/15/42/3155].Type-1 diabetes mellitus (T1DM) is associated with metabolic changes leading to alterations in glucose and lipid handling. While T1DM- associated effects on many major plasma lipids have been characterised, such effects on plasma free fatty acids (FFA) have not been fully examined. Using gas chromatography-mass spectrometry, we measured the plasma concentrations of FFA species in individuals with T1DM (n=44) and age/sex-matched healthy controls (n=44). Relationships between FFA species and various parameters were evaluated. Plasma concentrations of myristate (14:0), palmitoleate (16:1), palmitate (16:0), linoleate (18:2), oleate (18:1c9), cis-vaccenate (18:1c11), eicosapentaenoate (20:5), arachidonate (20:4) and docosahexanoate (22:6) were reduced in the T1DM group (p<0.0001 for all, except p=0.0020 for eicosapentaenoate and p=0.0068 for arachidonate); α-linolenate (18:3) and dihomo-γ- linolenate (20:3) concentrations were unchanged. Saturated/unsaturated FFA ratio, n-3/n-6 ratio, de novo lipogenesis index (palmitate (main lipogenesis product)/linoleate (only found in diet)) and elongase index (oleate/palmitoleate) were increased in the T1DM group (p=0.0166, p=0.0089, p<0.0001 and p=0.0008 respectively). The stearoyl-CoA desaturase 1 (SCD1) index 1 (palmitoleate/palmitate) and index 2 (oleate/stearate) were reduced in T1DM (p<0.0001 for all). The delta-(5)- desaturase (D5D) index (arachidonate/dihomo-γ-linolenate) was unchanged. Age and sex had no effect on plasma FFA concentrations in T1DM, while SCD1 index 1 was positively correlated (p=0.098) and elongase index negatively correlated with age (p=0.0363). HbA1c was negatively correlated with all plasma FFAs concentrations measured except α- linolenate and dihomo-γ-linolenate. Correlations were observed between plasma FFAs and cholesterol and HDL, but not LDL or diabetes duration. Collectively, these results aid our understanding of T1DM and its effects on lipid metabolism.Publisher PDFPeer reviewe

Adult Pulmonary Langerhans Cell Histiocytosis with Osseous Involvement: understanding this rare mimic of malignancy

Langerhans cells are dendritic cells that form the antigenic barrier of the human body. They occur in nearly any tissue but are most prevalent in the skin, submucosa of the bronchial tree, and other mucosae. Langerhans Cell Histiocytosis (LCH) develops when these cells damage the tissues in which they reside through a combination of inflammatory and monoclonal stimulation. The pulmonary variant of LCH involves the lung parenchyma and creates a wide variety of disturbances: pulmonary hypertension and both obstructive and restrictive lung disease. Osseous involvement, in addition to the pulmonary variant, presents with pulmonary masses and lytic bone lesions, which sparks suspicion for malignancy. Early recognition of this rare pathology is important as early treatment is clinically beneficial. The following explores a case of adult Pulmonary Langerhans Cell Histiocytosis with osseous involvement

Advancing qualitative rare disease research methodology: a comparison of virtual and in-person focus group formats

Background: Rare disease research is hampered in part by the fact that patients are geographically dispersed. Rare disease patient communities are recognized for their use of the internet to learn about their condition and find peer-to-peer support. As such, web-based technologies offer promise for overcoming geographic barriers in rare disease research for many. Qualitative focus groups (FGs) are a widely used methodology used to understand patients and parents/families ‘lived experience’ and unmet needs is important to improve care for rare diseases. It is unclear if web-enabled (virtual) FGs are comparable to traditional in-person approaches. We conducted in-person (n = 3) and virtual (n = 3) FGs with rare disease patients to determine if virtual FGs produce similar results in-person FGs. Results: Three in-person (n = 33 participants) and three virtual (n = 25 participants) FGs were conducted examining attitudes and beliefs regarding genetic testing and family communication of risk. Participants included 30 males, 18 females, and 10 parents/guardians. Two independent investigators identified excerpts (meaningful sections of text) and coded themes/sub-themes using a codebook. Inter-coder agreement across identified excerpts (n = 530) in both FG formats was 844/875 (96.5%). Two additional investigators reviewed coded excerpts and did not identify additional themes/sub-themes—supporting data saturation across FG formats. Virtual FGs accounted for 303/530 (57.2%) of total excerpts and 957/1721 (55.7%) of all identified themes/sub-themes. Formats were similar in terms of overall number of excerpts (101 ± 7.8 vs. 75.7 ± 18.8, p = 0.26) and themes/sub-themes (319 ± 6.1 vs. 254.7 ± 103.6, p = 0.34) between virtual and in-person FGs. However, virtual FGs had significantly more coded excerpts specifically relating to sensitive/intimate topics including ‘attitudes and beliefs’ (n = 320 vs. n = 235, p < 0.001), ‘information and support’ (n = 184 vs. n = 99, p < 0.001), and ‘family communication’ (n = 208 vs. n = 114, p < 0.001). Conclusions: Virtual FGs yielded similar numbers of coded excerpts compared to traditional in-person FGs. Virtual FGs appear to support the relative anonymity of participants, resulting in richer discussion of highly sensitive, intimate topics. Findings support the validity and methodologic rigor of using web-enabled technologies for conducting FGs in rare diseases