The RESET project: constructing a European tephra lattice for refined synchronisation of environmental and archaeological events during the last c. 100 ka

This paper introduces the aims and scope of the RESET project (. RESponse of humans to abrupt Environmental Transitions), a programme of research funded by the Natural Environment Research Council (UK) between 2008 and 2013; it also provides the context and rationale for papers included in a special volume of Quaternary Science Reviews that report some of the project's findings. RESET examined the chronological and correlation methods employed to establish causal links between the timing of abrupt environmental transitions (AETs) on the one hand, and of human dispersal and development on the other, with a focus on the Middle and Upper Palaeolithic periods. The period of interest is the Last Glacial cycle and the early Holocene (c. 100-8 ka), during which time a number of pronounced AETs occurred. A long-running topic of debate is the degree to which human history in Europe and the Mediterranean region during the Palaeolithic was shaped by these AETs, but this has proved difficult to assess because of poor dating control. In an attempt to move the science forward, RESET examined the potential that tephra isochrons, and in particular non-visible ash layers (cryptotephras), might offer for synchronising palaeo-records with a greater degree of finesse. New tephrostratigraphical data generated by the project augment previously-established tephra frameworks for the region, and underpin a more evolved tephra 'lattice' that links palaeo-records between Greenland, the European mainland, sub-marine sequences in the Mediterranean and North Africa. The paper also outlines the significance of other contributions to this special volume: collectively, these illustrate how the lattice was constructed, how it links with cognate tephra research in Europe and elsewhere, and how the evidence of tephra isochrons is beginning to challenge long-held views about the impacts of environmental change on humans during the Palaeolithic. © 2015 Elsevier Ltd.RESET was funded through Consortium Grants awarded by the Natural Environment Research Council, UK, to a collaborating team drawn from four institutions: Royal Holloway University of London (grant reference NE/E015905/1), the Natural History Museum, London (NE/E015913/1), Oxford University (NE/E015670/1) and the University of Southampton, including the National Oceanography Centre (NE/01531X/1). The authors also wish to record their deep gratitude to four members of the scientific community who formed a consultative advisory panel during the lifetime of the RESET project: Professor Barbara Wohlfarth (Stockholm University), Professor Jørgen Peder Steffensen (Niels Bohr Institute, Copenhagen), Dr. Martin Street (Romisch-Germanisches Zentralmuseum, Neuwied) and Professor Clive Oppenheimer (Cambridge University). They provided excellent advice at key stages of the work, which we greatly valued. We also thank Jenny Kynaston (Geography Department, Royal Holloway) for construction of several of the figures in this paper, and Debbie Barrett (Elsevier) and Colin Murray Wallace (Editor-in-Chief, QSR) for their considerable assistance in the production of this special volume.Peer Reviewe

Human occupation of Central Europe during the Last Glacial Maximum: new evidence from Moravia, Czech Republic

This article presents a brief examination of a recently discovered Last Glacial Maximum (LGM) site in Moravia. LGM sites are relatively rare in this part of Europe because it was abandoned by humans at the height of the last Ice Age due to decreasing temperatures and increasing aridity (Verpoorte 2004). Almost all ice sheets were at their LGM positions from 26.5ka to 19–20ka (Clark et al. 2009).One site that does date to this period is Mohelno, located close to the Jihlava River in the Czech-Moravian Highlands (Figure 1). At the time of occupation, it was situated near the bottom of a deeply incised river valley on a plateau c. 15–20m above the original level of the river. Steep slopes shielded the site from the north-east, north and west, forming a natural amphitheatre open to the south. This favourable position, and the heat-accumulating characteristics of the local rocks (orthogneiss, serpentinite), probably provided a ‘micro-oasis’ during the harsh climatic conditions of the LGM. Today, the site is situated below the water level of the Mohelno reservoir (Figure 2), which forms part of the Dalešice pumped-storage hydroelectric power station. Unique lithic artefacts and stone structures, interpreted as the remains of dwellings, reveal the complex character of the LGM occupation of Central Europ

A method for finding stratified sites: Early Upper Palaeolithic sites in Southern Moravia

There are several hundred recorded Early Upper Palaeolithic sites in Moravia, most of which are surface sites. The majority were exposed by agricultural plowing and subsequently discovered by pedestrian surveys whereas most of the stratified sites were found accidentally. Numerous unsystematic attempts in the past to find stratified remnants of sites disturbed by plowing have been unsuccessful. Here we present a methodology for locating stratified Early Upper Palaeolithic cultural contexts based on distribution of surface scatters. This involves pedestrian surveys guided by background research. All Palaeolithic artifacts were recorded using a handheld GPS with particular attention to calcium carbonate crust on artifact surfaces, which can be indicators of nearby stratified deposits. Exploratory test pits were then excavated followed by systematic excavations if the potential for stratified cultural deposits was deemed high. Using this technique, we have discovered 11 new stratified Early Upper Palaeolithic sites

Molecular cloning and characterization of the gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A.

The gene encoding the DNA methyltransferase, M.CviBIII, from Chlorella virus NC-1A was cloned and expressed in E. coli plasmid pUC8. Plasmid (pNC-1A.14.8) encoded M.CviBIII methylates adenine in TCGA sequences both in vivo in E. coli and in vitro. Transposon Tn5 mutagenesis localized the M.CviBIII functional domain to a 1.5 kbp region of pNC-1A.14.8 and also indicated that a virus promoter directs transcription of the gene in E. coli. The 2.1 kbp insert containing the M.CviBIII gene was sequenced and a single open reading frame of 1131 bp was identified within the domain determined by Tn5 mutagenesis. When the M.CviBIII gene was fused in-frame with the 19 amino-terminal codons of lacZ a 45 kD polypeptide was identified in maxicells as predicted by the DNA sequence. The M.CviBIII gene was not essential for virus replication since a virus M.CviBIII deletion mutant also replicated in Chlorella