Diminished production of TWEAK by the peripheral blood mononuclear cells is associated with vascular involvement in patients with systemic sclerosis.

Widespread vasculopathy and profound fibrosis are key features of the pathogenesis of systemic sclerosis (SSc). We hypothesized that the TNF-like weak inducer of apoptosis (TWEAK), a recently recognized multifunctional cytokine which regulates angiogenesis and tissue remodeling, may play a role in the development of SSc. The production of TWEAK by the peripheral blood mononuclear cells (PBMC) was investigated, by means of ELISA, in 24 SSc patients and 14 healthy subjects. Moreover, production of TWEAK was correlated with clinical features of SSc. PBMC were isolated using density gradient centrifugation on Histopaque and were cultured in FCS supplemented RPMI medium at 37 degrees C under 5% CO2. Production of TWEAK by PBMC was significantly diminished in patients with more severe microvascular damage, as indicated by the presence of "active" capillaroscopic pattern, compared with SSc patients with less pronounced microangiopathy ("slow" pattern), and healthy subjects. Moreover production of TWEAK correlated inversely with duration of Raynaud's phenomenon. PBMC from patients with scleroderma-related interstitial lung disease tended to produce lower amounts of TWEAK compared with SSc patients without lung involvement but the difference was not significant. The results of our study suggest that diminished production of TWEAK might play a role in the pathogenesis of vascular injury in SSc patients. Whether TWEAK may represent a new therapeutic target in SSc requires further studies

Investigation of Firebrand Generation from an Experimental Fire : Development of a Reliable Data Collection Methodology

An experimental approach has been developed to quantify the characteristics and flux of firebrands during a management-scale wildfire in a pine-dominated ecosystem. By characterizing the local fire behavior and measuring the temporal and spatial variation in firebrand collection, the flux of firebrands has been related to the fire behavior for the first time. This linkage is seen as the first step in risk mitigation at the wildland urban interface (WUI). Data analyses allowed the evaluation of firebrand flux with respect to observed fire intensities for this ecosystem. Typical firebrand fluxes of 0.82–1.36 pcs m−2 s−1 were observed for fire intensities ranging between 7.35±3.48 MW m−1 to 12.59±5.87 MW m−1. The experimental approach is shown to provide consistent experimental data, with small variations within the firebrand collection area. Particle size distributions show that small particles of area 0.75–5×10−5 m2 are the most abundant (0.6–1 pcs m−2 s−1), with the total flux of particles >5×10−5 m2 equal to 0.2–0.3 pcs m−2 s−1. The experimental method and the data gathered show substantial promise for future investigation and quantification of firebrand generation and consequently a better description of the firebrand risk at the WUI

Utilization of remote sensing techniques for the quantification of fire behavior in two pine stands

Quantification of field-scale fire behavior is necessary to improve the current scientific understanding of wildland fires and to develop and test relevant, physics-based models. In particular, detailed descriptions of individual fires are required, for which the available literature is limited. In this work, two such field-scale experiments, carried out in pine stands under mild conditions, are presented. A particular focus was placed on non-intrusive measurement, as the capabilities of advanced remote sensing techniques, along with more traditional approaches, are explored. A description of the fires is presented, with spread occurring predominantly in the surface fuels with intensities in the range of 200–4400 kW m-1, and punctuated by isolated regions of crown fire. The occurrence of crown fire is investigated and linked to regions of greater canopy density, and it is found that the total fire intensity may increase locally to as much as 21,000 kW m-1. The light winds do not appear to play a direct role in the changes in fire behavior, while fuel structure may be important. The measurements described herein provided a reasonable overall description of the fires, however, the current resolution (both spatial and temporal) falls short of definitively explaining some transitional aspects of the fire behavior, and future improvements are suggested

Investigation of firebrand production during prescribed fires conducted in a pine forest

This paper represents a study on the characterization of firebrand production which was carried out, using experimental fires conducted as prescribed fires in the New Jersey Pine Barrens, USA in March of 2013–2015. Several preliminary techniques were tested to characterize the firebrand production. Firebrands were collected from three plots for each year and analyzed for mass and size distribution. Thermal imagery was used to measure the velocity, size and number of firebrands in 2014 and 2015. The distribution of firebrands was evaluated in a monitored volume during the experiment. It was found that not less than 70% of collected particles were bark fragments and the rest were pine and shrub branches. The number of firebrands decreases with increasing the cross section area of firebrands. The mass of the particles varied from 5 to 50 mg, and the maximum number of the particles was observed for the mass range of 10–20 mg. About 80% of firebrands were particles with the cross section area of (5–20) × 10−5 m2. These results are consistent with the available observations of real fires [1], [2]. Processing of infrared video showed that starting from a distance of 13 m from fire front, an increasing number of firebrands were observed in a controlled volume, increasing in number up to 180 per second. Relationships describing the time-variation of the number of particles that dropped on a 1.4 m2 surface and the number of particles that flew through a 1 m3 volume were obtained. Comparing the experimental and calculated data, we can conclude that these relationships allow us to describe the two numbers with an acceptable accuracy (adj. R2 0.74 and 0.86, respectively). In addition, the velocity of the particles, which depended on the wind velocity, was in the 0.1–10.5 m/s range, with an average value of 2.5 m/s

Pandemic Influenza A H1N1 2009 Infection versus Vaccination: A Cohort Study Comparing Immune Responses in Pregnancy

Background: With the emergence of H1N1 pandemic (pH1N1) influenza, the CDC recommended that pregnant women be one of five initial target groups to receive the 2009 monovalent H1N1 vaccine, regardless of prior infection with this influenza strain. We sought to compare the immune response of pregnant women to H1N1 infection versus vaccination and to determine the extent of passive immunity conferred to the newborn. Methods/Findings: During the 2009-2010 influenza season, we enrolled a cohort of women who either had confirmed pH1N1 infection during pregnancy, did not have pH1N1 during pregnancy but were vaccinated against pH1N1, or did not have illness or vaccination. Maternal and umbilical cord venous blood samples were collected at delivery. Hemagglutination inhibition assays (HAI) for pH1N1 were performed. Data were analyzed using linear regression analyses. HAIs were performed for matched maternal/cord blood pairs for 16 women with confirmed pH1N1 infection, 14 women vaccinated against pH1N1, and 10 women without infection or vaccination. We found that pH1N1 vaccination and wild-type infection during pregnancy did not differ with respect to (1) HAI titers at delivery, (2) HAI antibody decay slopes over time, and (3) HAI titers in the cord blood. Conclusions: Vaccination against pH1N1 confers a similar HAI antibody response as compared to pH1N1 infection durin

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Compression d'images au moyen d'une modélisation par sinusoïdes exponentielles

Une méthode de modélisation d'images par sinusoïdes exponentielles est présentée. La séparabilité du problème est soulignée par la décomposition en valeurs singulières de l'image ce qui entraîne que les paramètres peuvent être déterminés à partir de deux signaux multicanal. Un schéma de compression d'images est élaboré comme une application possible de la modélisation