7 research outputs found
Applications de la spectroscopie ultrarapide Ă lâĂ©tude de sondes locales dâenvironnement et dâinteractions biomolĂ©culaires
In the context of the present thesis, UV-Vis time-resolved spectroscopy was applied targeting the photophysics investigation of different environmentally sensitive molecular systems, but also as a biosensing approach to reveal molecular interactions or structural dynamics on much slower time scales (sec to min), using droplet microfluidics triggering structural relaxation through out-of-equilibrium initial conditions. I thus investigated on a three-axis-target spanning from 1) the in-depth investigation of the emissive properties of biosensors in order to allow their quantitative use in biomolecular interaction studies, to 2) the development of an original experimental approach to enable resolving the structural relaxation kinetics of an out-of-equilibrium distribution of biomolecular structures, and finally 3) the technological application of time resolved fluorescence for precise, rapid, cost effective, biomolecular interaction assays, appealing both for academic and industrial arenas.La spectroscopie UV-Vis rĂ©solue en temps a Ă©tĂ© appliquĂ©e Ă l'Ă©tude de diffĂ©rents systĂšmes molĂ©culaires dont la photophysique est contrĂŽlĂ©e par leur interaction avec lâenvironnement, mais aussi comme outil pour rĂ©vĂ©ler des interactions molĂ©culaires ou des dynamiques structurelles Ă des Ă©chelles de temps comparativement lentes (sec Ă min.), en utilisant la microfluidique de gouttes pour dĂ©clencher une relaxation structurale par des conditions initiales hors Ă©quilibre. J'ai appliquĂ© cette approche selon trois axes allant de 1) l'Ă©tude approfondie des propriĂ©tĂ©s Ă©missives des biosenseurs afin de permettre leur utilisation quantitative dans les Ă©tudes d'interactions biomolĂ©culaires, Ă 2) le dĂ©veloppement d'une approche expĂ©rimentale originale pour permettre la rĂ©solution la cinĂ©tique de relaxation structurelle d'une rĂ©partition hors Ă©quilibre des structures biomolĂ©culaires, et enfin 3) l'application aux biotechnologies Ă haut dĂ©bit de la fluorescence rĂ©solue en temps pour des analyses d'interactions biomolĂ©culaires prĂ©cises et rapides, pertinente Ă la fois pour les domaines acadĂ©miques et industriels
Embedded Fluorescence Lifetime Determination for High-Throughput, Low-Photon-Number Applications
International audienc
Towards sensitive, high-throughput, biomolecular assays based on fluorescence lifetime
Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing Time Correlated Single Photon Counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by Time Correlated Single Photon Counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second
Design, Implementation and Characterization of Time-to-Digital Converter on Low-Cost FPGA
The fourth volume titled âSensors and Applications in Measuring and Automation Control Systemsâ contains twenty four chapters with sensor related state-of-the-art reviews and descriptions of latest advances in sensor related area written by 81 authors from academia and industry from 5 continents and 20 countries: Australia, Austria, Brazil, Finland, France, Japan, India, Iraq, Italia, MĂ©xico, Morocco, Portugal, Senegal, Serbia, South Africa, South Korea, Spain, UK, Ukraine and USA. Coverage includes current developments in physical sensors and transducers, chemical sensors, biosensors, sensing materials, signal conditioning, energy harvesters and sensor networks
Towards Sensitive, High-Throughput, Biomolecular Assays Based on Fluorescence Lifetime
Time-resolved fluorescence detection for robust sensing of biomolecular interactions is developed by implementing Time Correlated Single Photon Counting in high-throughput conditions. Droplet microfluidics is used as a promising platform for the very fast handling of low-volume samples. We illustrate the potential of this very sensitive and cost-effective technology in the context of an enzymatic activity assay based on fluorescently-labeled biomolecules. Fluorescence lifetime detection by Time Correlated Single Photon Counting is shown to enable reliable discrimination between positive and negative control samples at a throughput as high as several hundred samples per second
Ultrafast photophysics of the environment-sensitive 4âČ-methoxy-3-hydroxyflavone fluorescent dye
The ESIPT reaction speed of 4âČ-methoxy-3-hydroxyflavone varies by 3 orders of magnitude depending on the H-bonding capabilities of its environment.</p