Study of physiochemical contents in different types of surface Water from different area of Ghaziabad

The surface water forms the lifeline of almost all the human activities. The water pollution, inflow of solid waste, dumping of garbage in the drains and eroded soil, silt deposited in the natural drainage are major threat to surface water. The studies focusing on the assessment of changes in the river hydrology, morphology and water quality. The methodology used in the study involved assessment of Ghaziabad district water quality from six different locations for a period of one year 2016-17 in monsoon, winter and summer seasons. The parameters observed are Temperature, pH, Turbidity, Total Hardness (T-H), Calcium Hardness

2D-Molecular homology modeling of selected Enolase enzyme for Leishmaniasis

In present study, BLAST search was performed and an identity with Trypanosoma brueei is 79% Based upon and Pfam results Enolase- 2PTZ (protein data bank) was considered as an ideal homologue and used as a template for homology modeling due to its higher X-ray resolution at 1.65. Sequence alignment between enolase and 2PTZ was done using align 123 followed by manual modification. The final alignment was carefully evaluated and evidenced to be matching the conserved residue data for chain A of enolase in Trypanosoma brucei well Superimposing of the model was done over the template 2PTZ. Further research on the comparison of the models for the inhibitors may eluciadate the mechanism of enolase ligands interactions. This study has shown that some formation of favorable hydrogen bonds, hence it is predicated from this study that these novel compounds may act as potent inhibitors for Leishmania Enolase. These compounds may be used further for synthesis, their wet lab activity against Enolase, animal model study and clinical trials and then can be implicated for the treatment of leishmaniasis. Those models are considered to be used in designing new leads for hopefully more active compounds. Keywords: Enolase, Leishmaniasis, Enzyme, 2D-moleculare Modelin

Detection of non-tuberculous mycobacteria from sputum samples

NTM infections are an emerging global public health problem, especially in developing world. NTM and MTBC were proven to be responsible for various lungs, soft and skin tissues, and disseminated infections. Microscopy of AFB is rapid; however it does not discriminate between MTBC and NTM. Biochemical tests are time taking and interpretation of results may be difficult. The  GenoType®  Mycobacterium  CM/AS  assay  is reliable and rapid detection method in species of mycobacteria, which can  give  patients  with the onset  of  early targeted therapy. In present study, 189 patients detect by sputum sampling from total 3320 patients. Keywords: NTM, Sputum, Diagnosis, Clinical, Lucknow

Comparative analysis of different identification techniques for detection of mycobacterial species in clinical samples

Out of 906 cultures isolates, 263 (29.0%) were confirmed as NTM and 724 (71.0%) were identified as Mycobacterium tuberculosis complex. The diversity of NTM species was high and predominated by Mycobacterium abscessus (31.3%) followed by M. fortuitum (22%), M. intracellulare (13.6%), M. chelonae (9.1%), however, M. abscessus and M. fortuitum were the predominant species in both types of clinical isolates. Men (60.4%) and older patients aged greater than 55 years were the predominated risk group for NTM infection. Additionally, three HIV/AIDS cases (1.1%), as well as thirty five cases were found to be type two diabetes mellitus infected with non-tuberculous bacteria. Keywords: NTM, Technique, BacT/Alert, Biochemical Test, Tuberculosis

In-Silico evaluation of Anti-Leishmanial compounds of selected pharmacophore

In this study, out of sixteen compounds, only thirteen compounds have shown hydrogen bonding with the modeled cathepsin B protein of L donovani, remaining compound (C1D13416238, Posaconazole. and CID 6082033) did not show any hydrogen bonding with ligands. So, these 13 compounds which show hydrogen bonding with modeled protein could be considered as most potent lead compounds having inhibitory activity at either promastigote stage or amastigote stage of Leishmania donovani. Few compounds demonstrated better docking score to either AutoDock4.0 or GOLD v2.l but some compound did not show any hydrogen bonding with modeled protein in either docking software tool. Thus, it could be concluded that generated experimental compounds could have potential as pharmacological tool against Visceral Leishmaniasis. Keywords: Cysteine Protease, Vinyl Hydrazide, Antileishmanial Drugs, Licochalcone, Visceral Leishmaniasis

Virtual screening and molecular docking of Anti-Antileishmanial for selected pharmacophore for visceral Leishmaniasis

Objective: DNA amplification of Cysteine protease of Leishmania donovani and study the interaction of cysteine protease inhibitors, antileishmanial compounds with cysteine protease receptor in various computational programs. Materials and methods: Cysteine protease DNA of Leishmania donovani was amplified by PCR. The sequence of cysteine protease has been modeled and docked with suitable inhibitors by using various servers and computational tools. The model was designed, compared and validated by DOPE and Verify 3D scores. The model and the compound interaction were studied by LibDock and other programs. Results: Cysteine protease DNA of Leishmania donovani was successfully amplified by PCR. The structural modeling was done to achieve effective enzyme inhibition, inhibitors block the binding sites of that protein. Homology modeling of cysteine protease has been done and docked with suitable inhibitors by using various servers and computational tools. The model was designed, compared and validated by DOPE and Verify 3D scores by using DSv3.5. Licochalcone-a alone showed 37 LibDock conformations with 6 different poses, were suitably docked at the site 1 with hydrogen bond formation. The study would help to design the novel drugs in respect of resistant one for the treatment of harmful visceral Leishmaniasis. Conclusion: The molecular interaction of vinyl sulfones, hydrazide derivatives, antileishmanial drugs molecules and carbohydrazide derivatives have exhibited ideal molecular interaction with cathepsin B, a cysteine protease of L. donovani, amino acids such as Cys29, Hisl88 and Asn208 has been found to be active residues. Licochalcone-a and hydrazide derivative may become future antileishmanial compounds, which needs to be tested in in vitro and in vivo. Keywords: Cysteine Protease, Vinyl Sulfone, Hydrazide, Antileishmanial Drugs, Licochalcone, Visceral Leishmaniasis

Green HRM Practices and the Factors Forcing it: A Study on Health Care Entities in Chennai

Purpose: The purpose of this study is to examine the impact of Global Human Resource Management (GHRM) practices on the efficiency of healthcare organizations in the Chennai area. It aims to understand how GHRM can contribute to the success of these organizations, despite potential conflicts with their core values.   Design/Methodology/Approach: The study employs a research methodology that focuses on healthcare organizations within the Chennai area. It employs a comprehensive approach to analyze the implementation of GHRM practices in this specific context. The research design allows for an in-depth exploration of how GHRM practices influence the efficiency of these organizations.   Findings: The findings of this study reveal that GHRM practices have a substantial impact on the efficiency of healthcare businesses in Chennai. Despite potential conflicts with their core values, these organizations benefit from the advanced HRM strategies associated with GHRM. The study uncovers valuable insights into the role of GHRM in the healthcare industry.   Research, Practical & Social implications: This research has implications for both research and practice. It provides valuable insights for academics and researchers studying the impact of HRM practices on different industries, especially in contexts where traditional values may be challenged. Additionally, it offers practical guidance for healthcare organizations in Chennai and beyond, demonstrating the potential benefits of adopting GHRM practices. On a broader social scale, the study underscores the adaptability and relevance of GHRM in diverse sectors.   Originality/Value: The originality and value of this study lie in its specific focus on healthcare organizations in Chennai and their adoption of GHRM practices. By examining a niche sector within a specific geographic region, this research contributes to a deeper understanding of the applicability and benefits of GHRM. It adds to the body of knowledge by highlighting the adaptability of GHRM practices, even in industries with established core values, and underscores the potential for organizational success through strategic HR management

Wound Healing Ethnomedicinal Plants Popular among the Malayali Tribes in Vattal Hills, Dharmapuri, TN, India

Healing of chronic lower extremity wounds is a global problem, especially in developing countries where traditional medicine is often used by the people in remote places. India has a rich tradition of plant based knowledge pertinent to healthcare. A survey of ethnomedicinal plant species used by Malayali’s to heal cut/ wounds in Vattal Hills of Dharmapuri was made. A large number of plants/ extracts/ decoctions/ pastes are used by tribals to heal wounds, cuts and burns. In the present study, an attempt has been made to document ethnobotanical knowledge base and methods employed by Malayali’s for treatment of cut/ wounds. A large number of ethnomedicinal plants used by the Malayali’s have not been validated for wound healing potential. The present investigation resulted in the identification of 82 medicinal plant species distributed across 39 families that are used by Malayali’s to heal cut/ wounds. This study is an attempt to gather the information on the existing ethnobotanical knowledge base and document the traditional claims toward the development of safe of effective herbal drugs for cut/ wounds. Results of the study is organized in table form depicting the botanical name, family, vernacular name and habit with a brief note on plant parts used and method of administration

Case studies on heat stress related perceptions in different industrial sectors in southern India

Linkages between thermal loads and its physiological consequences have been widely studied in non-tropical developed country settings. In many developing countries like India, despite the widespread recognition of the problem, limited attempts have been made to estimate health impacts related to occupational heat stress and fewer yet to link heat stress with potential productivity losses. This is reflected in the ubiquity of workplaces with limited or no controls to reduce exposures. As a prelude to understanding the feasibility of alternative interventions in different industrial sectors, we present case studies from 10 different industrial units in Tamil Nadu, Chennai, which describe perceptions of occupational heat stress among the workers and supervisors/management

Association of acute toxic encephalopathy with litchi consumption in an outbreak in Muzaffarpur, India, 2014: a case-control study

Background Outbreaks of unexplained illness frequently remain under-investigated. In India, outbreaks of an acute neurological illness with high mortality among children occur annually in Muzaffarpur, the country’s largest litchi cultivation region. In 2014, we aimed to investigate the cause and risk factors for this illness. Methods In this hospital-based surveillance and nested age-matched case-control study, we did laboratory investigations to assess potential infectious and non-infectious causes of this acute neurological illness. Cases were children aged 15 years or younger who were admitted to two hospitals in Muzaffarpur with new-onset seizures or altered sensorium. Age-matched controls were residents of Muzaffarpur who were admitted to the same two hospitals for a non-neurologic illness within seven days of the date of admission of the case. Clinical specimens (blood, cerebrospinal fluid, and urine) and environmental specimens (litchis) were tested for evidence of infectious pathogens, pesticides, toxic metals, and other non-infectious causes, including presence of hypoglycin A or methylenecyclopropylglycine (MCPG), naturally-occurring fruit-based toxins that cause hypoglycaemia and metabolic derangement. Matched and unmatched (controlling for age) bivariate analyses were done and risk factors for illness were expressed as matched odds ratios and odds ratios (unmatched analyses). Findings Between May 26, and July 17, 2014, 390 patients meeting the case definition were admitted to the two referral hospitals in Muzaffarpur, of whom 122 (31%) died. On admission, 204 (62%) of 327 had blood glucose concentration of 70 mg/dL or less. 104 cases were compared with 104 age-matched hospital controls. Litchi consumption (matched odds ratio [mOR] 9·6 [95% CI 3·6 – 24]) and absence of an evening meal (2·2 [1·2–4·3]) in the 24 h preceding illness onset were associated with illness. The absence of an evening meal significantly modified the effect of eating litchis on illness (odds ratio [OR] 7·8 [95% CI 3·3–18·8], without evening meal; OR 3·6 [1·1–11·1] with an evening meal). Tests for infectious agents and pesticides were negative. Metabolites of hypoglycin A, MCPG, or both were detected in 48 [66%] of 73 urine specimens from case-patients and none from 15 controls; 72 (90%) of 80 case-patient specimens had abnormal plasma acylcarnitine profiles, consistent with severe disruption of fatty acid metabolism. In 36 litchi arils tested from Muzaffarpur, hypoglycin A concentrations ranged from 12·4 μg/g to 152·0 μg/g and MCPG ranged from 44·9 μg/g to 220·0 μg/g. Interpretation Our investigation suggests an outbreak of acute encephalopathy in Muzaffarpur associated with both hypoglycin A and MCPG toxicity. To prevent illness and reduce mortality in the region, we recommended minimising litchi consumption, ensuring receipt of an evening meal and implementing rapid glucose correction for suspected illness. A comprehensive investigative approach in Muzaffarpur led to timely public health recommendations, underscoring the importance of using systematic methods in other unexplained illness outbreaks