ANTI-INFLAMMATORY ACTIVITY OF TRAUMEEL (A HOMEOPATHIC PREPARATION) IN EXPERIMENTAL ANIMALS-RATS

 Objective: To evaluate the anti-inflammatory effect of traumeel on acute and chronic inflammatory experimental animal models (rats).Methods: The traumeel, a homeo medicinal preparation was tested for its efficacy in acute and chronic inflammatory rats. In acute models: Rat pawedema method was used. In this model, rats were divided into four groups, six rats each. Group-I (NC), received 2% GA 2 ml, Group-II (PC) receivedaspirin (200 mg/kg), Group-III, intravenous received low dose (6CH) and high dose (300CH) of traumeel, preparation orally. Edema was developed ininjected paw. Immediately the foot edema volume was measured at 0 hr and at the end of 3rd hr with a plethysmograph. The percentage of inhibitionof edema by drugs was measured and compared between the test, control and standard groups. In chronic models: Granuloma method was used,and test drugs were given for 7 days. The animals were divided and treated same as in the acute model. After 7 days treatment, on 8th day animalswere sacrificed each implanted pellet was extracted with adherent granulation tissue. These pellets were dried and weighed. The known weight wasdeducted from granulation tissue weight. The mean weight of granulation tissue and difference in weight of granulation tissue for each group wascalculated, and the percentage of inhibition of inflammation was calculated.Results: The percentage of inhibition of edema in the acute model with 6CH, 300CH after 3 hrs is 51%, 76% respectively and with aspirin is 79%. Inchronic models, the percentage of inhibition of granulation tissue with 6CH, 300CH after 7 days is 19.5%, 32.85%, respectively and with aspirin is41.95%. The reduction of inflammation was statistically significant in each group is (p<0.001).Conclusion: Traumeel, a homeo preparation is a best alternative drug for the treatment of acute inflammatory conditions with no side effects.Keywords: Inflammation, Traumeel preparation, Carrageenan, Aspirin.Â

Predictive maintenance and fault diagnosis of hydraulic gear coupling of a boiler feed pump unit

Increasing demand for reliability and performance of today’s most complex machines, and maintaining high productivity without sacrificing product quality have made it imperative for maintenance engineers to devise newer strategies in maintenance of plant and machinery. One of such strategies is condition monitoring, which has emerged as one of the most powerful technology in maintenance engineering. Condition monitoring and diagnostic engineering is a novel concept which enables us to detect in advance any incipient failure with ease and confidence in any part of a dynamic system before such failure trigger – off various types of failure mechanisms, which in turn may render the whole system uneconomical, unreliable, unhealthy, unsafe and even lethal. The present work highlights an experimental investigation to monitor the vibration behavior of hydraulic gear coupling, which is a part of Boiler Feed Pump train of a large utility Thermal Power Plant.  The coupling is supported by 4 Bearings. Tri-axial measurements are made at the bearing supports for 12 months. Displacement and Velocity are measured along Horizontal, Vertical and Axial directions. The experimental data is plotted on Time domain for graphical analysis to ease viewing of vibration signals. Based on the experimental data, faults are diagnosed using ISO – 2372 standards and causes are predicted. It is observed that the front and rear bearings of Input and output shafts of coupling are experiencing excess vibration. The work is concluded by suggesting remedial measures to ensure vibration intensity, at the said points, within the safe limits.&nbsp

Redox-regulated chaperone function and conformational changes of Escherichia coli Hsp33

We have studied the chaperone activity and conformation of Escherichia coli heat shock protein (Hsp)33, whose activity is known to be switched on by oxidative conditions. While oxidized Hsp33 completely prevents the heat-induced aggregation of ζ-crystallin at 42°C at a ratio of 1:1 (w/w), the reduced form exhibits only a marginal effect on the aggregation. Far UV–circular dichroism (CD) spectra show that reduced Hsp33 contains a significant α-helical component. Oxidation results in significant changes in the far UV–CD spectrum. Near UV–CD spectra show changes in tertiary structural packing upon oxidation. Polarity-sensitive fluorescent probes report enhanced hydrophobic surfaces in the oxidized Hsp33. Our studies show that the oxidative activation of the chaperone function of Hsp33 involves observable conformational changes accompanying increased exposure of hydrophobic pockets

TOXICITY EVALUATION OF AMMONIUM SULFATE TO ALBINO RAT

Objective: The present study was designed to find out the acute median lethal dose (LD50) of ammonium sulfate (inorganic fertilizer) in Wister albino rats.Methods: A single dose of ammonium sulfate dissolved in distilled water (Milli-Q) and administered intraperitoneally at concentrations of 10, 30, 50,70, 90, 110, 130, and 170 mg/kg body weight, respectively, to experimental animals, and then, they were observed every 3 hrs from prior dose giventime, later 6 hrs, 12 hrs, 24 hrs, to 48 hrs of noticing any abnormal behaviors and toxic signs, symptoms. After 48 hrs, counted the number of rats departed in each group and mortality percentage was calculated.Results: The obtained results were evaluated by the Statistical Probit Analysis Method and 48 hrs LD value for albino rats was found to be 91.5 mg/kg. At a single dose of 10 mg/kg, there is no morality and toxic behaviors were observed. Therefore, this concentration is considered as no observed adverse effect level dose. Conclusion: From the earlier consequences, identification and evaluation of the LD50 50 against ammonium sulfate is crucial for understanding thehyperammonemia because ammonium sulfate has been highly utilized as inorganic fertilizer in agriculture and household gardens. Thus, theknowledge about toxic impacts of ammonia useful for clinical or toxicological approaches; however, the toxicity data are unclear. Hence, the in vitroLD 50 evaluations of target chemical in Wistar rats is highly associated toward in ammonia-related peculiar disorders perceptive and therapy.Keywords: Ammonium sulfate, Fertilizer, Median lethal dose, Mortality, No observed adverse effect level.50 Objective:Thepresentstudywasdesignedtofindouttheacutemedianlethaldose(LD )ofammoniumsulfate(inorganicfertilizer)inWisteralbinorats.  Methods:Asingledoseofammoniumsulfatedissolvedindistilledwater(Milli-Q)andadministeredintraperitoneallyatconcentrationsof10,30,50,70, 90, 110, 130, and 170 mg/kg body weight, respectively, to experimental animals, and then, they were observed every 3 hrs from prior dose given time, later 6 hrs, 12 hrs, 24 hrs, to 48 hrs of noticing any abnormal behaviors and toxic signs, symptoms. After 48 hrs, counted the number of rats departed in each group and mortality percentage was calculated.  50 Results:  The  obtained  results  were  evaluated  by  the  Statistical  Probit  Analysis  Method  and  48  hrs  LD  value  for  albino  rats  was  found  to be91.5mg/kg.Atasingledoseof10mg/kg,thereisnomoralityandtoxicbehaviorswereobserved.Therefore,thisconcentrationisconsideredas no observed adverse effect level dose.  50 Conclusion:Fromtheearlierconsequences,identificationandevaluationoftheLD  againstammoniumsulfateiscrucialforunderstandingthe hyperammonemiabecauseammoniumsulfatehasbeenhighlyutilizedasinorganicfertilizerinagricultureandhouseholdgardens.Thus,the knowledgeabouttoxicimpactsofammoniausefulforclinicalortoxicologicalapproaches;however,thetoxicitydataareunclear.Hence,theinvitroLD 50 evaluations of target chemical in Wistar rats is highly associated toward in ammonia-related peculiar disorders perceptive and therapy. Keywords: Ammonium sulfate, Fertilizer, Median lethal dose, Mortality, No observed adverse effect leve

Effect of series resistance and interface state density on electrical characteristics of Au/SiO2/n-GaN Schottky diodes

metal-insulator-semiconductor (MIS) Schottky diodes and compared with (Au/n-GaN)metal-semiconductor (MS) Schottky diode. The effect of SiO2 on the surface preparation of n-GaN (MIS) Schottky diode is analyzed. The extracted Schottky barrier height and ideality factor of the MS Schottky diode is found to be 0.79 eV and 1.45 respectively. It is observed that the Schottky barrier height increases to 0.86 eV and ideality factor decreases to 1.3 for MIS diode. The interface state density as determined by Terman’s method is found to be 3.79 × 1012 and 3.41 × 1010 cm - 2 eV - 1 for the MS and MIS Schottky diodes, respectively. In addition, the values of series resistance (Rs) are determined using Cheung’s method. The I - V characteristics confirmed that the distribution of Nss, Rs and interfacial layer are important parameters that influence the electrical characteristics of MIS Schottky diodes. When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/2222

STABILITY-INDICATING VALIDATED REVERSED PHASE-HIGH PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS DETERMINATION OF COBICISTAT AND ATAZANAVIR SULFATE IN BULK AND PHARMACEUTICAL DOSAGE FORM

ABSTRACTObjective: A simple, rapid, precise, accurate, and economical stability-indicating reversed phase-high performance liquid assay method was developedand validated for simultaneous estimation of cobicistat (COB) and atazanavir (ATV) sulfate in bulk drugs and their combined commercial tablets.Methods: The method has shown adequate separation of COB and ATV from their degradation products. Separation was achieved on a LunaCN (250 mm × 4.6 mm, 5 μm column at a detection wavelength of 239 nm) using a mobile phase consists of o-phthaldialdeyde (Ph2.5) IX buffer,acetonitrile, and methanol in the ratio of 40:40:20 in an isocratic elution mode at a flow rate of 1 ml/min. Results: The retention times for COB and ATV sulfate were found to be 3.606 and 6.113 min, respectively. COB and ATV sulfate, their combinationdrug product was subjected to acid, base, neutral hydrolysis, thermal, and photolytic stress conditions. Thus, stressed samples were analyzed by theproposed analytical method. Validation of the proposed analytical method was carried out as per ICH guidelines Q2R1. Quantitation was achievedwith UV detection at 239 nm based on peak area with linear calibration curves at concentration ranges 50-600 μg/ml for COB and 100-1200 μg/ml forATV sulfate (R2 = 0.999 for both drugs). The limits of detection were 0.25 μg/ml and 0.5 μg/ml for COB and ATV sulfate, respectively. Conclusion: The method was found to be specific and stability indicating as no interfering peaks of degradants and excipients were observed. Theproposed method is hence suitable for application in quality-control laboratories for quantitative analysis of both the drugs individually and incombination dosage forms since it is simple and rapid with good accuracy and precision.Keywords: Stability-indicating assay, Reversed phase-high performance liquid, Cobicistat, Atazanavir sulfate, Forced degradation studies

Contribution of forest floor fractions to carbon storage and abundance patterns of arbuscular mycorrhizal fungal colonisation in a tropical montane forest

Forest floor carbon stocks, which include different components of litter, hemic and sapric materials, have not been empirically quantified in tropical montane forest, although they influence soil carbon (C) pools. To date, the contribution of arbuscular mycorrhizae in C sequestration potentials in tropical montane forests have not been clearly investigated. This study determined the amount of C stocks in the different decomposing layers of forest floor, mainly litter, hemic and sapric materials. The abundance of arbuscular mycorrhizal root colonisation differed among forest floor fractions. Forest floor was measured for depth, area density, dry mass and carbon fraction separately in Sungai Kial Forest Reserve, Pahang, Malaysia to calculate C stocks. Percentages of root colonisation in the hemic and sapric materials were investigated. The results showed that forest floor C stocks were significantly higher in hemic (5 Mg C ha−1) and sapric (7.7 Mg C ha−1) compared with the litter fragments (1.5 Mg C ha−1). Mycorrhizal root colonisation was significantly higher (75%) in the toeslope compared with the summit area in the hemic materials. Segregation of forest floor layers provided greater accuracy in forest floor C stocks reporting

Synthesis and Antimicrobial Activity of 3-[(4-Substituted) (2-oxo-1,3-oxazolidin-3-yl) phosphoryl]- 1,3-oxazolidin-2-ones

3-[(4-Substituted) (2-oxo-1,3-oxazolidin-3-yl)-phosphoryl]-1,3-oxazolidin-2-ones (4a–j) were synthesized through a two-step process. Bis-(2-oxo-1,3-oxazolidin-3-yl)-phosphonic chloride (2) prepared by the reaction of two moles of oxazolidin-2-one (1) with phosphorus oxychloride in dry tetrahydrofuran in the presence of triethylamine and treatment with various heterocyclic aromatic and aliphatic amines under the same experimental conditions afforded the title compounds (4a–j). They were characterized by elemental analysis, IR,NMR(1H, 13Cand 31P) and mass spectroscopy. Their antimicrobial activities were also evaluated.Keywords: 3-[(4-Substituted) (2-oxo-1,3-oxazolidin-3-yl)-phosphoryl]-1,3-oxazolidin-2-ones, oxazolidin-2-one, bis-(2-oxo-1,3-oxazolidin-3-yl)-phosphonic chloride, antimicrobial activity, spectral studie

Carica Papaya’s Anti-Diabetic and Anti-Cancer Properties – A Review

The Carica papaya originates from the Caricaceae family, and various members of this family have been used as treatments for a range of ailments. The perennial plant C. papaya, which is currently found over the whole tropical region, is thought to have originated in the southern region of Mexico. To assess the biological activity of distinct C. papaya sections, several scientific studies have been carried out. Since ancient times, the papaya plant's many components have been employed for medicinal purposes. In this article, the process of extracting Carica papaya leaves as well as the anti-cancer and anti-diabetic properties of papaya leaf activity were all things we wanted to assess. The information for this review paper, which focuses mostly on the therapeutic potential of papaya leaf extract was obtained via researching a collection of wider internet databases, including Google Scholar, PubMed, Science Direct, and Elsevier. The papaya plant, which has different parts such as fruit, leaves, seeds, bark, latex, and other substances, is very important in controlling the spread of illness. Alkaloids, glycosides, tannins, saponins, and flavonoids are just a few of the bioactive constituents in this, we focused on the papaya plant leaf's anti-cancer and anti-diabetic properties. The papaya has a wide range of therapeutic qualities. Papaya is a potent remedy, according to traditional beliefs. Biological activities have been the subject of much research. In the current review, all the pharmacological applications and activities of certain chemical components are discussed