Bimatoprost Effects on Aqueous Humor Dynamics in Monkeys

The effects of bimatoprost on aqueous humor dynamics were quantified in monkey eyes. Uveoscleral outflow was measured by the anterior chamber perfusion method, using FITC-dextran. Total outflow facility was determined by the two-level constant pressure method. Aqueous flow was measured with a scanning ocular fluorophotometer. Uveoscleral outflow was 0.96 ± 0.19 μL min−1 in vehicle-treated eyes and 1.37 ± 0.27 μL min−1 (n = 6; P < .05) in eyes that received bimatoprost 0.01% b.i.d. × 5 days. Bimatoprost had no effect on total outflow facility, which was 0.42 ± 0.05 μL min−1 at baseline and 0.42 ± 0.04 μL min−1 after bimatoprost treatment. Bimatoprost had no significant effect on aqueous humor flow. This study demonstrates that bimatoprost increases uveoscleral outflow but not total outflow facility or aqueous humor flow, indicating that it lowers intraocular pressure in ocular normotensive monkeys by a mechanism that exclusively involves uveoscleral outflow

http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-73954 Characterization of Shear-Sensitive Genes in the Normal Rat Aorta Identifies Hand2 as a Major Flow-Responsive Transcription Factor

Objective: Shear forces play a key role in the maintenance of vessel wall integrity. Current understanding regarding sheardependent gene expression is mainly based on in vitro or in vivo observations with experimentally deranged shear, hence reflecting acute molecular events in relation to flow. Our objective was to combine computational fluid dynamic (CFD

Технологическое оборудование

Содержит практические рекомендации по написанию, а также основные требования, предъявляемые к оформлению и защите курсовой работы. Для студентов специальности 1-36 01 03 «Технологическое оборудование машиностроительного производства» дневной и заочной форм обучения

Increased expression of VACM and TNF in regions exposed to low WSS.

<p>Expression of <i>VCAM1</i> (A) and <i>TNF</i> (B) in high and low systolic WSS regions in the rat aortic arch. Gene expression was analysed by real-time PCR and normalized to <i>TBP</i> mRNA expression prior analysis (paired T-test) (n = 14 pairs). WSS: wall shear stress.</p

Top 32 mechanosensitive genes in the rat aortic arch, classified according to function(s)/properties.

*<p>Down/up-regulated in low wall shear stress regions.</p

A strong differential expression between low and high WSS regions.

<p>Principal component analysis of Affymetrix Rat Gene 1.1 ST Array probe set data containing a total of 13 968 genes expressed in aortic tissue from 28 rat samples. Closed circles indicate low systolic WSS samples; open circles indicate high systolic WSS samples. The expression data was mean centred and scaled to unit variance prior analysis. WSS: wall shear stress.</p