Styloid Process Elongation or Eagle’s Syndrome: Is There Any Role for Ectopic Calcification?

The styloid process (SP) is a cylindrical, long cartilaginous bone located on the temporal bone. The normal SP length is approximately 20–30 mm. The styloid process elongation (SPE) can be assumed if either the SP or the adjacent stylohyoid ligament ossification shows an overall length in excess of 30 mm. Elongated SP is known as Eagle’s syndrome when it causes clinical symptoms as neck and cervicofacial pain. It is supposed that this symptoms and signs are due to the compression of the SP on some neural and vascular structures. It may also cause stroke due to the compression of carotid arteries. This syndrome is diagnosed by both radiographical and physical examination. Instead of many hypotheses and studies, the exact etiology of elongated SP and the role of ectopic calcification are unknown. Ectopic calcification (EC) might have a role for the elongation of SP. Abnormal calcium (Ca), phosphorus (P) and vitamin D metabolism is very common in patients with end-stage renal disease (ESRD). Therefore, this calcification in nonosseous soft tissue due to abnormal serum Ca and P levels is commonly associated with this disorder. EC due to the abnormality in this metabolism which is related to the duration of dialysis is also very important for this calcification. Therefore, a study in patients with ESRD investigating the prevalence of SP and the correlation between dialysis period and the SP length may help us explaining the role of EC in the elongation of SP. Because, this disease might be a good model for the investigation of the EC in this elongation. However, further studies and large samples are also needed to clarify the etiology of this disorder

Detection and partial characterization of two distinct walnut isolates of cherry leaf roll virus (CLRV)

Two new cherry leaf roll virus (CLRV) isolates (Ah and Ad) were isolated and detected from traditionally-grown walnuts that showed severe apical necrosis and chlorotic spots in systemically infected Cheneopodium amaranticolor, in the eastern part of Turkey. The 404 and 405 bp long DNA fragments of the 3’-non-coding region of both isolates from PCR reactions were cloned and sequenced. A significant genetic variability (up to 14% divergence between sequences) was found within the 3’ terminal region of viral genome of CLRV Turkish isolates compared with the isolates in databases. The sequence of Ad isolate was found to share 84 to 98% and the Ah isolate was found to share 85 to 97% nucleotide identity with corresponding sequences of the selected world isolates. An RNA riboprobe generated for CLRV-Ad isolate reacted also with the CLRV-Ah isolate in dot blot molecular hybridization test. Positive reactions were still visible in hybridization test when the extracts of infected fresh and dried leaf tissues of C. amaranticolor diluted 1:20. Western blot analysis revealed that the molecular mass of the coat protein of about 52 kDa for both isolates.Keywords: Walnut, CLRV, 3’ non coding regio

Studies on Parameters Influencing the Performance of Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) in Detecting Prunus Necrotic Ringpot Virus (PNRSV)

In order to have a more detailed understanding of the various factors influencing a reverse transcriptase polymerase chain reaction (RT-PCR), a number of important parameters such as Mg+2, primer, enzyme concentration and others were optimized for the detection of Prunus necrotic ringspot virus (PNRSV). Using a PNRSV isolate with a pair of primers, complementary DNA of viral genome as template, and an appropriate enzyme together with magnesium chloride, the following optimal conditions were identified: primer concentration between 0.2 and 0.0002 pmol µl-1 and 0.06–2 units µl-1 for Taq DNA polymerase enzyme for a 50 µl reaction volume when other parameters were optimum; magnesium chloride concentration less than 2.5 mM; dNTP concentration between 1 and 10 mM. The optimum cDNA amount should be ~360 ng for a 50 µl reaction mixture. When these optimized concentrations and/or values of the main PCR parameters were brought together for a new RT-PCR, a clear and a reliable PNRSV detection having no background was performed from both growth-chamber and field-grown PNRSV-infected plants

The impact of technology in hotels: A case study of Istanbul and Ankara hotels

[No abstract available

Variaciones en los contenidos de aceite, proteínas, ácidos grasos y vitamina E de las semillas de calabaza con riego deficitario

In the present study, pumpkin (Cucurbita Pepo L.) was grown under water stress to determine its effects on the chemical composition of the seeds (i.e., oil, protein, fatty acids and vitamin E), in Kayseri, Turkey. Irrigation treatments were designed to supply different portions of depleted moisture within the efficient root zone of the plants (60 cm). The treatments were arranged as supplying 100% (I100), 80% (I80), 60% (I60), 40% (I40), 20% (I20) and 0% (I0) of depleted moisture through a drip irrigation system. The effects of irrigation levels on the oil content of pumpkin seeds were found to be significant (p < 0.01). The oil contents of irrigation treatments varied between 26% (I0, dry) and 64% (I100, full irrigation). However, the effects of deficit irrigation on protein, fatty acids and vitamin E contents were not found to be significant. The vitamin E contents varied from 41.6 – 55.3 mg/100 g; while the protein contents varied from 28.5–37.7%. Six different fatty acids (linolenic, linoleic, oleic, stearic, palmitic and myristic acid) were examined. The average concentration of palmitic, stearic, oleic and linoleic acids ranged from 10.7–12.6%, 6.4–10.4%, 39.6–48.9% and 32.4–35%, respectively. Myristic and linolenic acids were not detected in the pumpkin seeds.En este trabajo se cultivaron calabazas (Cucurbita Pepo L.) en Kayseri, Turquía, con el objetivo de determinar los efectos del estrés hídrico en la composición química de las semillas (aceite, proteínas, ácidos grasos y vitamina E). Los tratamientos de irrigación se realizaron mediante el suministro de diferentes porciones de humedad dentro de la zona de la raíz eficiente de las plantas (60 cm). Los tratamientos se organizaron para suministrar 100% (I100), 80% (I80), 60% (I60), 40% (I40), 20% (I20) y 0% (I0) de humedad controlada a través del sistema de riego por goteo. Los efectos de los niveles de irrigación sobre el contenido de aceite de las semillas de calabaza fueron significativos (p < 0.01). El contenido de aceite en función de los tratamientos de riego varió entre el 26% (I0, seco) y el 64% (I100, riego completo). Sin embargo, los efectos del déficit de irrigación sobre los contenidos de proteínas, ácidos grasos y vitamina E no fueron significativos. Los contenidos de vitamina E variaron entre 41,6 y 55,3 mg/100 g, mientras que los contenidos de proteína variaron entre 28,5 y 37,7%. Se determinaron seis ácidos grasos (linolénico, linoleico, oleico, esteárico, palmítico y mirístico). La concentración promedio de los ácidos palmítico, esteárico, oleico y linoleico osciló entre 10,7–12,6%, 6,4–10,4%, 39,6–48,9% y 32,4–35%, respectivamente. Los ácidos mirístico y linolénico no fueron detectaron en las semillas de calabaza

Variaciones en los contenidos de aceite, proteínas, ácidos grasos y vitamina E de las semillas de calabaza con riego deficitario

In the present study, pumpkin (Cucurbita Pepo L.) was grown under water stress to determine its effects on the chemical composition of the seeds (i.e., oil, protein, fatty acids and vitamin E), in Kayseri, Turkey. Irrigation treatments were designed to supply different portions of depleted moisture within the efficient root zone of the plants (60 cm). The treatments were arranged as supplying 100% (I100), 80% (I80), 60% (I60), 40% (I40), 20% (I20) and 0% (I0) of depleted moisture through a drip irrigation system. The effects of irrigation levels on the oil content of pumpkin seeds were found to be significant (p < 0.01). The oil contents of irrigation treatments varied between 26% (I0, dry) and 64% (I100, full irrigation). However, the effects of deficit irrigation on protein, fatty acids and vitamin E contents were not found to be significant. The vitamin E contents varied from 41.6 – 55.3 mg/100 g; while the protein contents varied from 28.5–37.7%. Six different fatty acids (linolenic, linoleic, oleic, stearic, palmitic and myristic acid) were examined. The average concentration of palmitic, stearic, oleic and linoleic acids ranged from 10.7–12.6%, 6.4–10.4%, 39.6–48.9% and 32.4–35%, respectively. Myristic and linolenic acids were not detected in the pumpkin seeds.En este trabajo se cultivaron calabazas (Cucurbita Pepo L.) en Kayseri, Turquía, con el objetivo de determinar los efectos del estrés hídrico en la composición química de las semillas (aceite, proteínas, ácidos grasos y vitamina E). Los tratamientos de irrigación se realizaron mediante el suministro de diferentes porciones de humedad dentro de la zona de la raíz eficiente de las plantas (60 cm). Los tratamientos se organizaron para suministrar 100% (I100), 80% (I80), 60% (I60), 40% (I40), 20% (I20) y 0% (I0) de humedad controlada a través del sistema de riego por goteo. Los efectos de los niveles de irrigación sobre el contenido de aceite de las semillas de calabaza fueron significativos (p < 0.01). El contenido de aceite en función de los tratamientos de riego varió entre el 26% (I0, seco) y el 64% (I100, riego completo). Sin embargo, los efectos del déficit de irrigación sobre los contenidos de proteínas, ácidos grasos y vitamina E no fueron significativos. Los contenidos de vitamina E variaron entre 41,6 y 55,3 mg/100 g, mientras que los contenidos de proteína variaron entre 28,5 y 37,7%. Se determinaron seis ácidos grasos (linolénico, linoleico, oleico, esteárico, palmítico y mirístico). La concentración promedio de los ácidos palmítico, esteárico, oleico y linoleico osciló entre 10,7–12,6%, 6,4–10,4%, 39,6–48,9% y 32,4–35%, respectivamente. Los ácidos mirístico y linolénico no fueron detectaron en las semillas de calabaza

Carpal Tunnel Release Surgery and Venous Hypertension in Early Hemodialysis Patients without Amyloid Deposits

Aim. Carpal tunnel syndrome (CTS) is one of the frequent problems of the patients who underwent hemodialysis (HD). The role of venous hypertension due to arteriovenous fistula (AVF) has not been clarified completely; therefore, we aimed to investigate the role of venous hypertension due to AVF in hemodialysis patients who had CTS. Patients and Methods. We included 12 patients who had been receiving HD treatment for less than 8 years and the newly diagnosed CTS patients with the same arm of AVF. All patients were diagnosed clinically and the results were confirmed by both nerve conduction studies and electromyography. Open carpal tunnel release surgery was performed on all of them. Venous pressure was measured in all patients before and after two weeks of surgery. Results. There were significant differences before and after the surgery with regard to pressures (). After the surgery, all carpal ligament specimens of the patients were not stained with Congo red for the presence of amyloid deposition. Conclusion. Increased venous pressure on the same arm with AVF could be responsible for CTS in hemodialysis patients. Carpal tunnel release surgery is the main treatment of this disease by reducing the compression on the nerve

Rabies Prophylaxis for Pregnant Women

Rabies Prophylaxis for Wome

Mediterranean-Black Sea gateway exchange: Scientific drilling workshop on the BlackGate project

The MagellanPlus workshop "BlackGate"addressed fundamental questions concerning the dynamic evolution of the Mediterranean-Black Sea (MBS) gateway and its palaeoenvironmental consequences. This gateway drives the Miocene-Quaternary circulation patterns in the Black Sea and governs its present status as the world's largest example of marine anoxia. The exchange history of the MBS gateway is poorly constrained because continuous Pliocene-Quaternary deposits are not exposed on land adjacent to the Black Sea or northern Aegean. Gateway exchange is controlled by climatic (glacio-eustatic-driven sea-level fluctuations) and tectonic processes in the catchment as well as tectonic propagation of the North Anatolian Fault Zone (NAFZ) in the gateway area itself. Changes in connectivity trigger dramatic palaeoenvironmental and biotic turnovers in both the Black Sea and Mediterranean domains. Drilling a Messinian to Holocene transect across the MBS gateway will recover high-amplitude records of continent-scale hydrological changes during glacial-interglacial cycles and allow us to reconstruct marine and freshwater fluxes, biological turnover events, deep biospheric processes, subsurface gradients in primary sedimentary properties, patterns and processes controlling anoxia, chemical perturbations and carbon cycling, growth and propagation of the NAFZ, the timing of land bridges for Africa and/or Asia-Europe mammal migration, and the presence or absence of water exchange during the Messinian salinity crisis. During thorough discussions at the workshop, three key sites were selected for potential drilling using a mission-specific platform (MSP): one on the Turkish margin of the Black Sea (Arkhangelsky Ridge, 400mb.s.f., metres below the seafloor), one on the southern margin of the Sea of Marmara (North Imrali Basin, 750mb.s.f.), and one in the Aegean (North Aegean Trough, 650mb.s.f.). All sites target Quaternary oxic-anoxic marl-sapropel cycles. Plans include recovery of Pliocene lacustrine sediments and mixed marine-brackish Miocene sediments from the Black Sea and the Aegean. MSP drilling is required because the JOIDES Resolution cannot pass under the Bosporus bridges. The wider goals are in line with the aims and scope of the International Ocean Discovery Program (IODP) "2050 Science Framework: Exploring Earth by Scientific Ocean Drilling"and relate specifically to the strategic objectives "Earth's climate system", "Tipping points in Earth's history", and "Natural hazards impacting society"