ANTIOXIDANT, CYTOTOXICITY, AND STABILITY EVALUATION OF GINKGO BILOBA EXTRACT-BASED MICROEMULSIONS FOR ENHANCED THERAPEUTIC ACTIVITY

Objective: This study is aimed to evaluate the antioxidant (AO) potential, cytotoxicity, and stability of preformulated Ginkgo biloba standard extractmicroemulsion (GBME), to investigate if, it retains the therapeutic potential of EGB761 and remains safe and stable for a longer period.Method and Results: GBME has shown enhanced AO (85.2±0.78%, IC50=31.3±0.45 μg/ml) in comparison to EGB761 (74.1±0.51%,IC50=49.4±0.05 μg/ml) using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay. Similarly, 2,2-diphenyl-1-picryl-hydrazyl-hydrate(DPPH) assay has also shown that AO for GBME (94.6±0.04%, IC50=11.4±1.03 μg/ml) was higher than EGB761 (78.6±1.20%, IC50=34.6±0.81 μg/ml).Further, IC50 value of antiradical unit of GBME was much lesser (ABTS=14.3±1.05 μg/ml and DPPH=17.03±1.8 μg/ml) in comparison to EGB761(ABTS=34.1±1.62 μg/ml and DPPH=37.5±0.08 μg/ml). Equivalently, both, hydrogen peroxide scavenging activity, and nitric oxide activity wereappreciably higher for GBME than the pure extract. The in vitro cytotoxicity assessment showed that GBME is quite safe (98.68±0.76% cell viability) incomparison to EGB761 (83.29±1.02%). Thereafter, these samples were tested for stability by evaluating their AO activity along with high-performanceliquid chromatography analysis, for the major phytocompounds, after 1 year, and results suggested that AO of GBME remained stable while comparingwith the freshly prepared GBME, whereas AO of EGB761 reduced significantly as compared to freshly taken EGB761 extract implying the degradationof phytocompounds supporting decrease in AO activity.Conclusion: Therefore, the observed results suggest that GBME maintained AO and scavenging activity along with enhanced shelf life with no observedtoxicity, which can be explored further for its potential therapeutic implications in various oxidative stress-induced central nervous system disorders

Evaluation of the ecological state of the major streams located in Doon valley using diatoms as indicators

Biological communities reflect overall ecological integrity (i.e. chemical, physical and biological integrity). Since the diatom species exhibit variation in tolerances to physical and chemical change in water, they are excellent indicators changes in their environment. Based on the dominant categories for each characteristic, the ecological state of the stream can be determined. This study evaluates the ecological state of the streams in Doon Valley. The diatom samples collected at regular monthly intervals from the twelve streams located in the eastern and western Doon were treated with acid-peroxide and mounted in Naphrax for species count at x1500. About 250-300 valves of diatom species were counted and Van Dam ecologic values were computed by OMNIDIA ver. 5.3. pH was alkaliphilous (4), salinity - fresh brackish (2) and oxygenation - continuously high (1) for most of the streams. Saprobity and trophic status was largely ?-mesosaprobous (2) and eutraphentic (5), respectively. The Leclercq index also shows non-existent (<10%) to low (10-20%) organic pollution, degradation (IDSE=3.5-3.0) and moderate anthropogenic eutrophication (20.1-45%) in most of the streams attributed to urbanization stress (62 MLD sewage). The water quality based on indices; IBD (Indice biologique diatomèes), IPS (Indice de polluo-sensibilite) and TDI (Trophic Diatom Index) showed the moderate water quality in the streams of the Valley. Like EU Water Framework Directive 2000/60/EC (WFD) India should also have the objective to ensure ecosystem integrity through bio-assessment and not just mere pollution control

Flow equations in commercial gas piping system

There are various types of flow equations in commercial gas piping system and this thesis will covered about the comparison of Cox and Pole‟s method. FORTRAN 90 software was used to validate the manual calculation of both equations and a comparative study was made. The analytical solutions of the resulting differential equations are obtained in the form of Cox and Pole‟s equations. The equations give functional relationship between flow rate, inlet pressure and outlet pressure, as well as pressure drop at any given length. The effect of pressure drop per segment on gas flow rate is presented. Both of the equation was calculated manually from the commercial area gas piping route and the syntax generated from the equations were compiled and executed in F90 with no errors. The Cox‟s method was found more economically with smaller pipe size but allowable pressure drop in each section, compared to Pole‟s method. The equations considerably enhance gas pipeline design in terms of both ease of usage and accuracy. A simple computer program in FORTRAN 90 is developed to handle these calculations

Managing dub with progesterone - locally or orally which is a better option?

Background: The levonorgestrel intrauterine system (LNG-IUS) is a safe and effective form of contraception used by millions of people worldwide. Other than this, it has many non-contraceptive benefits-treatments for dysfunctional uterine bleeding (DUB), fibroid uterus, endometriosis and endometrial hyperplasia.Methods: A prospective longitudinal comparative study was carried out at department of obstetrics and gynaecology S.N. Medical College, Agra, Uttar Pradesh, India including 100 women of 20-45 years of age group (comparable in all aspects), with DUB. All cases were subjected to detailed history, examination and baseline investigations- Hemoglobin (Hb), endometrial aspiration, histopathology and ultrasound pelvis (along with endometrial thickness) and PBAC scoring before starting the treatment. Then cases were randomly allocated in two equal groups. In group A LNG-IUS was inserted. In group B cases were prescribed oral medroxy progesterone acetate 10 mg bd from 5th and 25th day of menstrual cycle. Cases were followed at 1 month, 3 months and 6 months after starting treatment. At each follow-up visit primary outcome in terms of subjective assessment by patient, PBAC scores and secondary outcome as Hb levels and side-effects were recorded.Results: Reduction in PBAC Scores, improvement in Hb and reduction in endometrial thickness were seen with both treatment modalities but results were significantly (p <0.0001) better with LNG-IUS group as compared to MPA. LNG-IUS was found to be more effective in endometrial hyperplasia and proliferative type of endometrium.Conclusions: LNG-IUS is a good alternative to oral progesterone therapy for patients of DUB

Material Study of Co2CrAl Heusler Alloy Magnetic Thin Film and Co2CrAl/n-Si Schottky Junction Device

The structural, optical, magnetic, and electrical properties of Co2CrAl Heusler alloy magnetic thin films grown on n-type silicon (100) substrate (n-Si) and glass substrate were studied. The films were deposited using DC magnetron sputtering. X-ray diffraction (XRD) analysis confirmed the polycrystalline nature of the films. The effect of grain size on transmittance was investigated. Magnetic measurements revealed the presence of magnetic ordering in the films. Partial densities of states (PDOS) of the Co2CrAl were calculated by density functional theory (DFT) methods using the Vienna Ab initio Simulation Package (VASP). Co2CrAl thin film deposited over a silicon substrate was investigated for I-V characteristics. The electrical behaviour confirmed the existence of a Co2CrAl/n-Si Schottky contact, which suggests a spin injection phenomenon from Co2CrAl to n-Si by tunnelling through the lowered Schottky barrier

NICU Poster - 2019

NICU Poster - 2019https://scholarlycommons.libraryinfo.bhs.org/research_education/1006/thumbnail.jp

Oral lesions and immune status of HIV infected adults from eastern Nepal

Objective: To document the prevalence, age and gender distribution of oral lesions in HIV infected adults and the influence of highly active antiretroviral therapy and correlate them to the immune status of the patients. Materials and Methods: Oral lesions were diagnosed by a detailed physical examination by trained and calibrated examiners according to the case definitions established by the Oral HIV/AIDS research alliance. Demographic details, risk behavior patterns and oral symptoms and habits were collected by a questionnaire. Results: 81 patients; 54 men and 27 women aged between 20 ' 55 years participated in the study. A total of 49 patients; 60.5% had some oral lesion when examined. Oral candidiasis (21 %) and oral melanosis (21%) were the most common lesions, followed by linear gingival erythema, oral hairy leukoplakia, necrotizing ulcerative periodontitis/ gingivitis, herpes labialis, parotid gland enlargement and reccurent apthous ulcers. Oral hairy leukoplakia was exclusively seen in men (p=0.018). All six cases of herpes simplex lesion were seen in non - anti retro viral group (p=0.073) while oral candidiasis was commonly noted in the anti retro viral group (p=0.073). Lowering CD4 counts had the strongest association with the prevalence of oral candidasis (p=0.012), pseudomembranous candidiasis (p=0.014) and oral hairy leukoplakia (p= 0.065). Conclusion: This study shows a high prevalence of oral candidiasis, melanosis, linear gingival erythema and oral hairy leukoplakia in the patients