Component traits influencing seed yield in recombinant inbred lines of lentil (Lens culinaris Medik.)

One hundred and thirty five RILs (Recombinant Inbred Lines) developed from a cross between an indigenous and exotic line of lentil (Lens culinarisMedik.)were evaluated for seed yield and component traits during rabi 2012-13 and 2013-14. Pooled analysis of variance revealed significant differences among the RILsfor all the traits studied. This suggested that there was ample scope for selection of promising RILs for yield improvement in lentil. Phenotypic and genotypic coefficients of variation were high for pods per plant(32.49% and 26.75%) followed by biological yield per plot(24.38% and 21.28%). Genetic advance was highest for 100-seed weight(47.75%)followed by pods per plant(45.39%). Estimation of phenotypic correlation coefficients indicated that seed yield per plot expressed highly significant and positive correlation with biological yield per plot(0.634), harvest index(0.300) and seeds per pod(0.156). Path coefficient analysis revealed that the traits; biological yield per plot , harvest index, number of pods per plant, days to 50% flowering, days to maturity , plant height and primary branches per plant had positive direct effect on seed yield per plot. The selection of these traits would be helpful for further yield improvement in lentil

Optimization of process variables for enhanced lactic acid production utilizing paneer whey as substrate in SMF

In the present study lactic acid production was enhanced by optimizing the three process variables viz; inoculum size, temperature and pH using three factor five level CCRD (central composite rotatable design) by Lactobacillus delbruckii under SMF (submerged fermentation process). Paneer (dairy by-product) whey was used as sole substrate for lactic acid production. Design Expert 8.0.2.0 software depicted that an optimum concentration of 8% (v/v) size of inoculum, 5.50 pH and 36.53C temperature gave lactic acid and biomass yield of 5.61 g/L and 4.27 g/L, respectively. Lactic acid production was scale up in 7.5 L bioreactor under optimized conditions and it gave lactic acid and biomass yield of 39.2±1.4 and 47.6±0.8 g/L, respectively. μg, YP/S, YP/X and productivity were found to be 0.14 h-1, 0.66 g/g, 0.7 g/g and 1.98 g/L. h, respectively. Leudking Piret equation deduced that lactic acid production was growth associated which varies from earlier reports. Lactic acid was characterized by FTIR (Fourier transform infrared spectroscopy) and HPLC (High performance liquid chromatography)

Assessment Of Purisha At Laboratory Level- A Review

Ahara is panchabhautika, each element of ahara is not used by thebody, thus it is eliminated in the form of waste(Mala). The importanceof by products, produced during the digestion process, is as importantas food consumption in terms of nutrition for every body segment andthese phenomena are unavoidable. The proper formation of these malasis a reflection of the normal metabolic function and regular dhatuproduction, with good characteristics. Mala have the samephysiological value as dosha or dhatu, since they are considered to bebody moola. The metabolism will be affected if malas are not removedfrom the body, eventually leading to a malformation of tissues anddisease

Comparative study between pre- and post- operative clinical and electrophysiological parameters in determining the recovery of carpal tunnel syndrome

Background: Carpal tunnel syndrome (CTS) is a neuropathy caused by entrapment of the median nerve at the wrist. CTS is the most well-known and frequent form of median nerve entrapment and accounts for 90% of all entrapment neuropathies. CTS, in the form of median nerve entrapment, remains a perplexing and debilitating disorder. We report a comparative study between the Boston questionnaire (BQ) and median nerve conduction study parameters in patients with CTS treated by division of the flexor retinaculum through a short incision at the palm.Methods: The prospective cross-sectional, hospital-based study was conducted in department of burns, plastic and reconstructive surgery at SCB Medical College, Cuttack.Results: The average age was 44.49 years (SD=10.51; range=24-70), and the average symptom duration was 19.9 months (range=6-36). There are 36 hands in the severe group, 22 with the moderate group, and 12 with the mild category. The mean symptom severity scale score for severe group A was 40.92 (SD=5.84) and for moderate group B was 31.67 (SD=5.72), and for the mild group, C was 26.0 and SD=3.82). We found significant correlation between symptomatic recovery and Boston severity scales.Conclusions: Clinical correlation of pre- and post-op symptoms is the only full proof way to predict recovery after surgery in CTS‑affected, patients

Redox signalling to nuclear regulatory proteins by reactive oxygen species contributes to oestrogen-induced growth of breast cancer cells

Background: 17β-Oestradiol (E2)-induced reactive oxygen species (ROS) have been implicated in regulating the growth of breast cancer cells. However, the underlying mechanism of this is not clear. Here we show how ROS through a novel redox signalling pathway involving nuclear respiratory factor-1 (NRF-1) and p27 contribute to E2-induced growth of MCF-7 breast cancer cells. Methods: Chromatin immunoprecipitation, qPCR, mass spectrometry, redox western blot, colony formation, cell proliferation, ROS assay, and immunofluorescence microscopy were used to study the role of NRF-1. Results: The major novel finding of this study is the demonstration of oxidative modification of phosphatases PTEN and CDC25A by E2-generated ROS along with the subsequent activation of AKT and ERK pathways that culminated in the activation of NRF-1 leading to the upregulation of cell cycle genes. 17β-Oestradiol-induced ROS by influencing nuclear proteins p27 and Jab1 also contributed to the growth of MCF-7 cells. Conclusions: Taken together, our results present evidence in the support of E2-induced ROS-mediated AKT signalling leading to the activation of NRF-1-regulated cell cycle genes as well as the impairment of p27 activity, which is presumably necessary for the growth of MCF-7 cells. These observations are important because they provide a new paradigm by which oestrogen may contribute to the growth of breast cancer

A triple action CDK4/6-PI3K-BET inhibitor with augmented cancer cell cytotoxicity

National Institutes of Health GM125195, GM135671, CA192656, FD00511

Efforts and strategies for alleviation of drought tolerance in chickpea in India

Among the various cool season pulses grown in India, chickpea (Cicer arietinum L.) occupies an important position in rainfed agriculture. Chickpea is largely grown (>67% area) on conserved/ residual moisture, contributing about 45% to the total pulses production in India. Being a rainfed crop, it often faces terminal drought stress. When chickpea sowing is delayed in fields vacated by paddy in the presence of terminal heat, soil moisture stress further limits its productivity. This necessitates the adoption of suitable strategies to alleviate per se drought tolerance in chickpea. Two main strategies, integrated breeding and transgenic technology, have been adopted for developing drought-tolerant varieties. The third potential strategy is utilization of nanotechnology, which has not been tried under Indian NARES. Systematic breeding efforts resulted in development of varieties having per se drought tolerance (RSG 44, Vijay, RSG 888, JG 74, Pusa 2024), and escape (JG 11, JG 16, JSC 56, IPC 2006-77 etc.) exploiting earliness. QTLs responsible for drought tolerance have been identified in chickpea genotype ICC 4958, which have been transferred, and drought-tolerant elite breeding lines have been developed. MAGIC and NAM populations combining drought and heat tolerance are now becoming available for identification of elite breeding lines having combined tolerance to both stresses for their possible release. Exploitation of transgenic technology using DREB gene has also shown promise. The use of nano-particles in enhancing root development and growth vigour in chickpea has opened the doors for exploitation of nanotechnology for alleviation of per se drought tolerance

Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma

<p>Abstract</p> <p>Background</p> <p>Oral malignancy is a major global health problem. Besides the main risk factors of tobacco, smoking and alcohol, infection by human papillomavirus (HPV) and genetic alterations are likely to play an important role in these lesions. The purpose of this study was to compare the efficacy of HC-II assay and PCR for the detection of specific HPV type (HPV 16 E6) in OSMF and OSCC cases as well as find out the prevalence of the high risk HPV (HR-HPV) in these lesions.</p> <p>Methods and materials</p> <p>Four hundred and thirty patients of the potentially malignant and malignant oral lesions were taken from the Department of Otorhinolaryngology, Moti Lal Nehru Medical College, Allahabad, India from Sept 2007-March 2010. Of which 208 cases were oral submucous fibrosis (OSMF) and 222 cases were oral squamous cell carcinoma (OSCC). The HC-II assay and PCR were used for the detection of HR-HPV DNA.</p> <p>Result</p> <p>The overall prevalence of HR-HPV 16 E6 DNA positivity was nearly 26% by PCR and 27.4% by the HC-II assay in case of potentially malignant disorder of the oral lesions such as OSMF. However, in case of malignant oral lesions such as OSCC, 32.4% HPV 16 E6 positive by PCR and 31.4% by the HC-II assay. In case of OSMF, the two test gave concordant result for 42 positive samples and 154 negative samples, with an overall level of agreement of 85.4% (Cohen's kappa = 66.83%, 95% CI 0.553-0.783). The sensitivity and specificity of the test were 73.7% and 92.05% (p < 0.00). In case of OSCC, the two test gave concordant result for 61 positive samples and 152 negative samples, with an overall level of agreement of 88.3% (Cohen's kappa = 79.29, 95% CI 0.769-0.939) and the sensitivity and specificity of the test were 87.14% and 92.76% (p < 0.00).</p> <p>Conclusion</p> <p>This study concluded that slight difference was found between the positivity rate of HR-HPV infection detected by the HC-II and PCR assay in OSMF and OSCC cases and the HC II assay seemed to have better sensitivity in case of OSCC.</p

Effect of participatory women's groups facilitated by Accredited Social Health Activists on birth outcomes in rural eastern India: A cluster-randomised controlled trial

Background: A quarter of the world's neonatal deaths and 15% of maternal deaths happen in India. Few community-based strategies to improve maternal and newborn health have been tested through the country's government-approved Accredited Social Health Activists (ASHAs). We aimed to test the effect of participatory women's groups facilitated by ASHAs on birth outcomes, including neonatal mortality. Methods: In this cluster-randomised controlled trial of a community interve