The Potential to Build Collective Capacity for Organisational Learning in the Context of Teachers’ Use of Digital Technology for School Improvement

This article considers how digital spaces focused on whole school improvement combined with supportive leadership may be mobilised towards building collective capacity for evidence-informed practice and organisational learning. This topic originated from a qualitative, multi-method design-based research (DBR) project that studied practitioners’ use of an online resource for primary school practitioners called Pathways for school improvement, designed by Oxford University Press (OUP). Semi-structured interviews, participant observations and a documentary analysis were conducted with teachers and senior leaders in five primary schools across England between 2014 and 2016. Connections were made with the dynamic approach to school improvement (DASI) that encourages practitioners to systematically engage with a variety of evidence in their reflections and efforts to design school and classroom improvement strategies. Pathways’ four-step system and series of systematised tasks under each step seemed to provide opportunities for practitioners to explore elements of theory and practice in conjunction with empirical and pupil performance data, and potentially guide them through how to collaborate with others in developing specific whole school approaches to improvement. Opportunities and challenges in developing collective capacity for improvement are also explored

Magnetic Properties of Gadolinium-Doped ZnO Films and Nanostructures

The magnetic properties of Gd-doped ZnO films and nanostructures are important to the development of next-generation spintronic devices. Here, we elucidate the significant role played by Gd-oxygen-deficiency defects in mediating/inducing ferromagnetic coupling in in situ Gd-doped ZnO thin films deposited at low oxygen pressure by pulsed laser deposition (PLD). Samples deposited at higher oxygen pressures exhibited diamagnetic responses. Vacuum annealing was used on these diamagnetic samples (grown at a relatively high oxygen pressures) to create oxygen-deficiency defects with the aim of demonstrating reproducibility of room-temperature ferromagnetism (RTFM). Samples annealed at oxygen environment exhibited superparamagnetism and blocking-temperature effects. The samples possessed secondary phases; Gd segregation led to superparamagnetism. Theoretical studies showed a shift of the 4f level of Gd to the conduction band minimum (CBM) in Gd-doped ZnO nanowires, which led to an overlap with the Fermi level, resulting in strong exchange coupling and consequently RTFM

Prospecting for scarabid specific Bacillus thuringiensis crystal toxin cry8 gene in sugarcane ecosystem of Tamil Nadu, India

In the present study, we report the occurrence of cry8 positive isolates of Bacillus thuringiensis (Bt) in selected white grub, Holotrichia serrata F. (Coleoptera: Scarabaeidae), endemic soils of sugarcane ecosystem and other places in Tamil Nadu. Out of the 66 soil samples collected and screened for white grub specific Bt, 74 isolates of the bacterium, all containing only spherical crystal toxin, were identified. PCR screening of these isolates with cry8 gene universal primer revealed six isolates to be positive. Further, the amplicon of a 370 bp band, amplified with another set of degenerate primer designed based on the conserved sequence of cry8 genes, was sequenced from four isolates. Multiple sequence alignment revealed the gene sequences to be the same for all the isolates. The present report of the availability of cry8 positive Bt isolates opens the avenue for controlling white grubs through transgenic research

Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe

<p>Abstract</p> <p>Background</p> <p>Hepatitis C virus (HCV) poses a growing threat to global health as it often leads to serious liver diseases and is one of the primary causes for liver transplantation. Currently, no vaccines are available to prevent HCV infection and clinical treatments have limited success. Since HCV has a small proteome, it relies on many host cell proteins to complete its life cycle. In this study, we used a non-directed phenyl sulfonate ester probe (PS4≡) to selectively target a broad range of enzyme families that show differential activity during HCV replication in Huh-7 cells.</p> <p>Results</p> <p>The PS4≡ probe successfully targeted 19 active proteins in nine distinct protein families, some that were predominantly labeled <it>in situ </it>compared to the <it>in vitro </it>labeled cell homogenate. Nine proteins revealed altered activity levels during HCV replication. Some candidates identified, such as heat shock 70 kDa protein 8 (or HSP70 cognate), have been shown to influence viral release and abundance of cellular lipid droplets. Other differentially active PS4≡ targets, such as electron transfer flavoprotein alpha, protein disulfide isomerase A5, and nuclear distribution gene C homolog, constitute novel proteins that potentially mediate HCV propagation.</p> <p>Conclusions</p> <p>These findings demonstrate the practicality and versatility of non-directed activity-based protein profiling (ABPP) to complement directed methods and accelerate the discovery of altered protein activities associated with pathological states such as HCV replication. Collectively, these results highlight the ability of <it>in situ </it>ABPP approaches to facilitate the identification of enzymes that are either predominantly or exclusively labeled in living cells. Several of these differentially active enzymes represent possible HCV-host interactions that could be targeted for diagnostic or therapeutic purposes.</p

Green synthesis of magnesium oxide nanoparticles and their antibacterial activity

1210-1215Nanotechnology has prospects of opening new avenues to fight and prevent diseases using atomic-scale tailoring of materials. As the nano revolution emerges, it is imperative to develop “nano‑naturo” links between nanotechnology and green domains of the nature. The present investigation describes the mangrove Rhizophora lamarckii’s property of synthesizing magnesium oxide nanoparticles . The newly synthesized magnesium oxide nanoparticle morphology is nanohexagonal and spherical. The particles range in dimensions between 20 and 50 nm and are crystalline in nature. The functional groups of the mangrove, amine, and alkane are found to act as reductants and stabilizers. The newly synthesized MgO nanoparticles are found to have potent antibacterial activity

EmLog:Tamper-Resistant System Logging for Constrained Devices with TEEs

Remote mobile and embedded devices are used to deliver increasingly impactful services, such as medical rehabilitation and assistive technologies. Secure system logging is beneficial in these scenarios to aid audit and forensic investigations particularly if devices bring harm to end-users. Logs should be tamper-resistant in storage, during execution, and when retrieved by a trusted remote verifier. In recent years, Trusted Execution Environments (TEEs) have emerged as the go-to root of trust on constrained devices for isolated execution of sensitive applications. Existing TEE-based logging systems, however, focus largely on protecting server-side logs and offer little protection to constrained source devices. In this paper, we introduce EmLog -- a tamper-resistant logging system for constrained devices using the GlobalPlatform TEE. EmLog provides protection against complex software adversaries and offers several additional security properties over past schemes. The system is evaluated across three log datasets using an off-the-shelf ARM development board running an open-source, GlobalPlatform-compliant TEE. On average, EmLog runs with low run-time memory overhead (1MB heap and stack), 430--625 logs/second throughput, and five-times persistent storage overhead versus unprotected logs.Comment: Accepted at the 11th IFIP International Conference on Information Security Theory and Practice (WISTP '17

Influence of photon beam energy on IMRT plan quality for radiotherapy of prostate cancer

BackgroundIntensity-modulated radiation therapy (IMRT) has been widely used for prostate cancer treatments. 6MV photon beams were found to be an effective energy choice for most IMRT cases. The use of high-energy photons raise concerns about increased leakage and secondary neutron dose for the patients.AimIn this work, the effect of beam energy on the quality of IMRT plans for prostate radiotherapy was systematically studied for competing IMRT plans optimized for delivery with either 6 or 10MV beams.Materials and MethodsA cohort of 20 prostate cases was selected for this study. All patients received full-course IMRT treatments to a dose of 79.2Gy to PTV in 44 fractions. For all of the cases we developed treatment plans using 6 MV and 10MV intensity-modulated beams with identical dose volume constraints.ResultsPercentage of doses received by the percentage volume of PTV was higher for 6MV photons compared to 10MV photons for 12 patients, less than or equal to 1% for 6 patients and 2.6%, 3.6% for the remaining 2 patients irrespective of the PTV volume. Percentage doses received by 15% of bladder volume were higher for 10 MV photons. Percentage doses received by 15% of rectum volume were also higher for 10 MV photons.ConclusionsSince there is no greater advantage from 10MV photons as compared with 6MV photons in large volume pelvic IMRT dosimetry and also 10MV photons lie on the threshold energy border for the induction of photo neutrons from the accelerator components, we recommend the use of 6MV photons for IMRT of prostate cancer to achieve better results in tumour control and acceptable probability of complication rate

Influence of recycled basalt-aramid fibres integration on the mechanical and thermal properties of brake friction composites

In the brake friction composites(BFCs), fibres take part in significant attention as reinforcement in governing mechanical and thermal-mechanical properties. The current investigation aims to develop hybrid brake friction composites using recycled basalt- aramid fibre integration and to characterise for its mechanical and thermal properties. The experiments related to thermal (heat swell, loss of ignition and thermal conductivity) and mechanical (tensile, compression, flexural and impact) properties were conducted as per industrial standards. From the experimental investigations, it was concluded that fibre inclusion in the BFCs enhanced the mechanical and thermal properties considerably. Further, with the aid of scanning electron microscope (SEM), fracture interfaces of the tested friction composites were analyzed for various characteristics like pullout, void, fibre-matrix bonding etc

Whole genome analysis and functional characterization of a novel Bacillus thuringiensis (Bt 62) isolate against sugarcane white grub Holotrichia serrata (F)

In this study, we report the whole genome assembly of Bt 62, a novel isolate harbouring cry8 holotype gene identified by us earlier. Sequencing was carried out using a combination of Illumina NextSeq 500 and Oxford Nanopore sequencing Technologies (ONT). The final assembled genome was 6.13 Mb comprising a circular chromosome and four plasmids. The bioassay studies against Holotrichia serrata (F.) (Coleoptera: Scarabaeidae), a polyphagous pest infesting sugarcane and other crops, indicated significant toxicity to first instar grubs over untreated larvae achieving a highest mean mortality of 91.11% for various doses tested. In vitro proteolytic assay and histopathological studies of the midgut of infected white grubs revealed proteolytic processing of the protoxin and extensive degeneration of larval midgut epithelial cells. The results demonstrate that this novel isolate could be used as a biopesticide or its crystal toxin genes could be expressed in sugarcane and other crops for resistance against H. serrata

Hyperoxic Treatment Induces Mesenchymal-to-Epithelial Transition in a Rat Adenocarcinoma Model

Tumor hypoxia is relevant for tumor growth, metabolism and epithelial-to-mesenchymal transition (EMT). We report that hyperbaric oxygen (HBO) treatment induced mesenchymal-to-epithelial transition (MET) in a dimetyl-α-benzantracene induced mammary rat adenocarcinoma model, and the MET was associated with extensive coordinated gene expression changes and less aggressive tumors. One group of tumor bearing rats was exposed to HBO (2 bar, pO2 = 2 bar, 4 exposures à 90 minutes), whereas the control group was housed under normal atmosphere (1 bar, pO2 = 0.2 bar). Treatment effects were determined by assessment of tumor growth, tumor vascularisation, tumor cell proliferation, cell death, collagen fibrils and gene expression profile. Tumor growth was significantly reduced (∼16%) after HBO treatment compared to day 1 levels, whereas control tumors increased almost 100% in volume. Significant decreases in tumor cell proliferation, tumor blood vessels and collagen fibrils, together with an increase in cell death, are consistent with tumor growth reduction and tumor stroma influence after hyperoxic treatment. Gene expression profiling showed that HBO induced MET. In conclusion, hyperoxia induced MET with coordinated expression of gene modules involved in cell junctions and attachments together with a shift towards non-tumorigenic metabolism. This leads to more differentiated and less aggressive tumors, and indicates that oxygen per se might be an important factor in the “switches” of EMT and MET in vivo. HBO treatment also attenuated tumor growth and changed tumor stroma, by targeting the vascular system, having anti-proliferative and pro-apoptotic effects