23 research outputs found

    Étude ethnobotanique des plantes alimentaires utilisées en médecine traditionnelle dans la région Maritime du Togo

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    Les plantes alimentaires contiennent des principes actifs douĂ©s de diverses propriĂ©tĂ©s mĂ©dicinales pouvant intervenir dans le traitement de nombreuses maladies. Cette Ă©tude est consacrĂ©e au recensement des plantes ayant des potentiels nutritionnel et thĂ©rapeutique dans la rĂ©gion Maritime du Togo, dans le but de la valorisation de ces plantes. De juin Ă  septembre 2017, une enquĂŞte ethnobotanique, basĂ©e sur l’utilisation des interviews individuelles Ă  l'aide d'un questionnaire semi-structurĂ©, a Ă©tĂ© rĂ©alisĂ©e auprès de 101 Praticiens de la MĂ©decine Traditionnelle. Au total 86 espèces vĂ©gĂ©tales appartenant Ă  72 genres et 36 familles ont Ă©tĂ© identifiĂ©es. Les Fabaceae et les Solanaceae (7 espèces chacune) ont Ă©tĂ© les plus reprĂ©sentĂ©es. Les espèces les plus citĂ©es ont Ă©tĂ© Ocimum gratissimum L. (10,48%), Vernonia amygdalina Delile (6,71%), Lactuca taraxacifolia (Willd.) Schum. (6,08%) et Heliotropium indicum L. (5,66%). Les feuilles (77,85%), les fruits (5,63%) et les racines (4,26%) sont les organes les plus utilisĂ©es sur 799 recettes inventoriĂ©es. La principale forme galĂ©nique reste la sauce (51,19%) et le mode principal d’administration est la voie orale (90,74%). Concernant les maladies traitĂ©es, les affections du tube digestif sont au premier rang (43,80%) suivies par des affections cardiovasculaires (13,52%). Cette Ă©tude fournie une base de donnĂ©es sur des plantes ayant des potentiels nutritionnel et thĂ©rapeutique au Togo.Mots clĂ©s : Alicaments, potentiels nutritionnel et thĂ©rapeutique, sĂ©curitĂ© alimentaire, Togo.   English title: Ethnobotanical study of some food plants used in traditional medicine in the Maritime region of TogoFood plants contain active substances with various medicinal properties that can be used to treat many diseases. This study is devoted to the inventory of plants with nutritional and therapeutic potential in Maritime region of Togo, with the aim of promoting these plants. From June to September 2017, an ethnobotanical survey was conducted among 101 Traditional Medicine Practitioners through individual interviews using a semi-structured questionnaire. A total of 86 plants species belonging to 72 genera and 36 families were identified. Fabaceae and Solanaceae (7 species each) were the most represented. The most cited species were Ocimum gratissimum L. (10.48%), Vernonia amygdalina Delile (6.71%), Lactuca taraxacifolia (Willd.) Schum. (6.08%) and Heliotropium indicum L. (5.66%). The leaves (77.85%), fruits (5.63%) and roots (4.26%) were the most plant parts used out of 799 inventoried recipes. The main dosage form remains the sauce (51.19%) and the main mode of administration is the oral route (90.74%). Regarding the treated diseases, the digestive disorders are in first place (43.80%), followed by cardiovascular diseases (13.52%). This study provides a database of plants with nutritional and therapeutic potential in Togo.Keywords: Food plants, nutritional and therapeutic potentials, food security, Togo

    Evaluation In Vitro De L’activité Antimicrobienne Des Extraits De Cassia Alata Linn. (Fabaceae)

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    Cassia alata (Linn) is a Togolese flora plant traditionally used in the treatment of skin diseases and diarrhea. The objective of this work was to evaluate the in vitro antimicrobial activity and to highlight certain phytochemical total and fractionated extracts of this plant harvested in southern Togo. These extracts were obtained from polar solvents such as water, ethanol and ethanol / water mixture in equal volume. Microbial strains used consisted of bacteria, Staphylococcus aureus, Escherichia coli and Klebsiella oxytoca and yeasts, Candida albicans and Candida krusei. The antimicrobial activity was evaluated by the liquid medium dilution method coupled to spread on solid medium. Highlighting chemical groups was made by a brief qualitative phytochemical analysis from staining tests. The results show that the ethanol leaves crude extract (EBE) was the most active of all the tested microbial strains. This extract completely inhibited the growth of S. aureus (MIC = 1.25mg/ ml.); very strongly C. albicans (PI = 94.34 % ) and C. krusei (PI = 90.67% ) and strongly E. coli ( PI = 80%) and K. oxytoca (PI=79.14 %). The other extracts were active in some organisms with percentage inhibition (PI) of between 68 and 97 %. The phytochemical screening of some extracts revealed the presence of flavonoĂŻdes, tannins and saponins. C. alata seems to contain compounds that interact to inhibit the growth of yeasts and bacteria. These results in part to justify the use of this plant in the Togolese traditional medicines

    Therapeutic potentials of ethanolic extract of leaves of Holarrhena floribunda (G. Don) Dur. And schinz (apocynaceae)

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    Background: Holarrhena floribunda is a plant of wide usage in the Togolese folk medicine. A previous ethnobotanical survey on the latex plants of the Maritime region of the country revealed that this plant was included in several recipes curing malaria and microbial infections. Therefore, this study aimed to seek for the effectiveness of the ethanolic extract of the plant in the treatment of these diseases.Methods: The antimicrobial test was performed using the agar well-diffusion and the NCCLS broth microdilution methods, while the in vivo antimalarial activity was evaluated following the four-day suppressive test of Peters. The acute toxic effects of the extract were monitored after a single oral dose (5,000 mg/kg body weight) administration in NMRI mice.Results: The results indicated that the ethanolic extract of leaves of H. floribunda was active on Staphylococcus aureus ATCC 29213 and clinical strains of Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae with MICs ranging from 0.62 to 1.25 mg/mL. The extract also showed significant parasitaemia suppression in a dose-dependent manner. In the acute toxicity assay, the oral administration of the extract to the mice did not affect the relative weight of vital organs, and there were no signs of toxicity or death during the study period. The LD50 of the tested extract was found to be greater than 5,000 mg/kg, indicating its safety.Conclusion: This study demonstrates the antibacterial and antimalarial activities of leaves of H. floribunda and then, supports its medicinal use in the treatment of microbial infections.Keywords: Holarrhena floribunda, ethanolic extract, antibacterial, antimalarial, toxicit

    Ethnobotanical Study Of Medicinal Plants In The Fight Against Buruli Ulcer In The Maritime Region Of Togo

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    Background: In Togo, few data are available on the traditional treatment of Buruli ulcer, so this study aim to inventory and identify the plants and recipes included in the herbal medicines in the maritime region where this disease is more common. Method: The methodology was based on ethnobotanical semi-structural individual interviews of 16 traditional healers in the maritime region. The Data were collected and analyzed in Microsoft excel 2013. Results: 10 of the traditional healers were male and the average age of all the respondents was 42.31 ± 4.24 years. A total of 27 plant species belonging to 20 families were identified. The most represented families were: Caesalpinaceae with 3 species, Euphorbiaceae, Apocynaceae, Asteraceae, Sapindaceae and Annonaceae with 2 species each. In the preparation methods, the decoction is the most used (37%), followed by the black powdered incorporated in an ointment (24%), the maceration (15%), the infusion (12%), the spraying (6%) and poultices (6%). These modes of preparation, were administered mainly externally by massage, disinfection of the lesion and orally. The most used parts of the plants were leaves (48%), roots (21%), and bark (10%). The species with the highest frequency of use were Blighia sapida KD Koenig, Amaranthus spinosus L, Ocimum canum Sims, Piliostigma thonningii (Schum.) Milne-Redh, Aloe verra L, Anacardium occidentale L, Paullinia pinnata L and Xylopia aethiopica (Dunal) A.Rich. Conclusion: By highlighting these plant species of interest, this study has an applicability in therapeutic innovation. It can contribute to the chemical, pharmacological and clinical evaluations of their molecules leading to development of phytomedicines to produce new drugs to strengthen the already existing therapeutic arsenal

    Diagnostic moléculaire du complexe Mycobacterium tuberculosis résistant à l’isoniazide et à la rifampicine au Burkina Faso

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    Introduction: cette étude a eu pour objectifs de diagnostiquer la tuberculose pulmonaire par l'examen microscopique et par la PCR des crachats et de déterminer les bases moléculaires de la résistance à la rifampicine et à l'isoniazide. Méthodes: le diagnostic du Complexe Mycobacterium Tuberculosis (CMTB) a été effectué par microscopie après coloration au Ziehl Nielsen et par PCR en temps réel en utilisant le kit d'identification du complexe MTB (Sacace Biotechnologie, Italie). Les résistances à la Rifampicine et à l'Isoniazide ont été étudiées par la technique de la PCR en utilisant le kit MTB résistance 8 (Sacace, Biotechnologie). Résultats: sur les 59 patients diagnostiqués pour la tuberculose pulmonaire, 59,3% étaient positifs en microscopie optique et 44,1% étaient positifs par PCR en Temps réel. Les résistances à la rifampicine (rpoB) et à l'isoniazide (katG et inhA) ont été observées chez 9 patients. La résistance à la rifampicine était due aux mutations (Asp516Val, Ser531Trp, Leu533Pro) et celle à l'isoniazide par les substitutions Ser315Thr du gène katG et C209T du gène inhA. Les multi résistances à la rifampicine et à l'isoniazide ont été observées dans 55,5% des échantillons et concernaient les associations : ropBAsp513Val + inhAC209T et rpoBLeu533Pro + katGSer315Thr. Conclusion: la PCR en temps réel qui permet l'identification des allèles mutants rpoB, katG et inhA de M. tuberculosis est un outil de diagnostic épidémiologique de grande importance car elle permet de déterminer le niveau de résistance à la rifampicine et à l'isoniazide.Keywords: Mycobacterium tuberculosis, résistance, rifampicine, isoniazid

    Molecular characterization of high-risk humanpapillomavirus genotypes in women with or without cervical lesions at VIA/VILI in Kara, Togo

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    Background: Persistent infection with high-risk (HR) papillomavirus (HPV) genotypes plays a central role in the pathogenesis of invasive cervical cancer. Objectives: This study aimed to determine the prevalence and distribution of HR-HPV among women with or without cervical lesions at VIA/VILI in Togo. Methods: Cervical samples were collected from 238 women with or without cervical lesions at VIA / VILI and[c3] DNA [c4]was extracted and analyzed by real-time multiplex PCR. Logistic regression analysis was used to determined risk factors associated with HPV infection. inPietro Annigoni Biomolecular Research Center (CERBA / LABIOGENE) in Burkina Faso. Results: The age of the women ranged from 17 to 61 years old, and most were married (73.5%). The prevalence of HRHPV was 35.71% and this was higher in the age range 35-39 years. The six most common genotypes were HPV 31 (18.7%), HPV 52 (13.82%), HPV 68 (13.01%), HPV 66 (9.76%), HPV 58 (8.13%) and HPV 56 (8.13%). Genotypes HPV 18 (4.07%)and HPV 16 (0.81%) were less frequent.[c5] Married or living with a partner was associated with HPV infection ( OR=2,17, IC [1.20-3.91], p<0,009). Conclusion: This study allowed characterizing for the first time in Togo, HR-HPV genotypes. This will help mappingHR-HPV genotypes circulating in West Africa. Keywords: Human papillomavirus; High-risk; Genotyping; Kara; Togo

    Is a mass drug administration deworming programme for school-aged children enough to reduce the prevalence of soil-transmitted helminths and Schistosoma mansoni in adults: a cross-sectional study from Togo

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    BackgroundThe world health organization (WHO) introduced the mass drug administration (MDA) strategy in order to reduce the prevalence of soil-transmitted helminths (STHs) and Schistosoma mansoni in endemic areas. However, this strategy is not implemented in adult population in Togo. Thus, the question arose if the present MDA strategy contributes to the reduction of transmission rates. The present study aimed to monitor the prevalence of STHs and S. mansoni among adult’s, between 2017 and 2022, in the central region of Togo.MethodsTwo cross-sectional studies were conducted in six villages in the central region of Togo in 2017 and 2022. Stool samples were collected from adults over the age of 18 years. To assess STHs and S. mansoni infections, real-time multiplex qPCR and Kato-Katz techniques were performed. Data were analysed using SPSS software version 21 and GraphPad PRISM version 9.2.0. A p-value less than 0.05 was considered statistically significant.ResultsA total of 210 and 289 individuals were recruited in 2017 and 2022, respectively. We detected significant increase in the prevalence of STHs and S. mansoni from 7.61% to 24.56% (p=0.0008) and from 27.62% to 46.36% (p=0.0014) by Kato-Katz and RT-qPCR, respectively. The prevalence of Ancylostoma duodenale infection was the highest with an increase from 7.14% to 23.53% by Kato-Katz and 9.09% to 40.0% by RT-qPCR.ConclusionThe prevalence of STHs and S. mansoni increased in the adult population in the central region of Togo from 2017 to 2022, despite the implementation of MDA in school-aged children. Hence, there is an urgent need to include adult individuals and adapt the MDA programme in the central region of Togo

    Relationship between texture and cell-wall components of safou (Dacryodes edulis (G. Don) H.J. Lam) fruits at different storage conditions

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    Objective: The objective of this study was to determine the relationship between texture and cell wall component of safou (Dacryodes edulis (G Don) HJ Lam) at different storage conditions during the safou softening.Methodology and results: Using a General Linear Model (GLM), statgraphic software plus v. 5. 0 and XLstat 2007software, the relationship between texture and cell wall component of safou at different storage conditions was evaluated. At different stages of ripeness, safou were picked and stored in different conditions including: cultivar, picking mode, packaging mode and temperature. For each condition, texture, water loss, alcoholinsoluble CDTA- soluble pectins and neutral sugar level and methoxylation degree were measured.The results showed that the temperature and method of storage and theirs interaction influenced significantly the texture of the safou during storage. Softening and dehydration through transpiration are the two processes that changed the safou texture at high temperature (28°C and 36°C). The cultivar and the maturity stage also influenced significantly the texture. The correlation between texture and cell-wall component including alcohol insoluble solids, CDTA soluble pectin extracts, rhamnose and arabinose were very high. MIA (Materiel Insoluble in Alcohol) content increased and galacturonic acid content of CDTA Soluble Pectin extracts decreased, depending on the maturity level of safou: the degradation of homogalacturonans of pectins during the safou softening.Conclusion and application of results: the degradation of homogalacturonans of safou pectins had an impact on their texture. Therefore, for their postharvest shelf life, safou must be stored at low temperature (18°C) with or without packing.Keywords: Safou, texture, pectins, softening, Dacryodes eduli

    Évaluation du plan de maitrise sanitaire dans les unités de production d’eau en sachet au Togo: cas des villes de Kara et Sokodé

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    Pour faire un état des lieux sur les mesures de maîtrise sanitaire dans les unités de production des eaux conditionnées en sachets, une enquête a été réalisée à Kara et Sokodé. La méthode d’échantillonnage a été exhaustive. Le questionnaire administré a porté sur les caractéristiques des unités de production d’eau, sur les bonnes pratiques d’hygiène et de fabrication et sur les plans de surveillance et de contrôle. Pour compléter l’enquête, les critères du Règlement 178/2002/CE de l’Union Européenne relatifs aux eaux de consommation ont été utilisés pour l’analyse microbiologique de 13 échantillons d’eau prélevés dans ces unités. Les résultats ont montré que le plan de maîtrise sanitaire se résume seulement aux bonnes pratiques d’hygiène et de fabrication appliquées seulement dans 4 unités sur les 10. L’accent est mis sur le suivi médical des employés et le contrôle des qualités bactériologique et physico-chimique qui sont semestriels au lieu d’être mensuels. Les analyses microbiologiques ont révélé la présence de Streptocoques fécaux dans un échantillon d’eau et de coliformes totaux dans un autre. Ce qui indique un manquement aux règles d’hygiène. La mise en place du système HACCP dans les unités de production des eaux garantirait mieux leur sécurité sanitaire

    THERAPEUTIC POTENTIALS OF ETHANOLIC EXTRACT OF LEAVES OF HOLARRHENA FLORIBUNDA (G. DON) DUR. AND SCHINZ (APOCYNACEAE)

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    Background: Holarrhena floribunda is a plant of wide usage in the Togolese folk medicine. A previous ethnobotanical survey on the latex plants of the Maritime region of the country revealed that this plant was included in several recipes curing malaria and microbial infections. Therefore, this study aimed to seek for the effectiveness of the ethanolic extract of the plant in the treatment of these diseases. Methods: The antimicrobial test was performed using the agar well-diffusion and the NCCLS broth microdilution methods, while the in vivo antimalarial activity was evaluated following the four-day suppressive test of Peters. The acute toxic effects of the extract were monitored after a single oral dose (5,000 mg/kg body weight) administration in NMRI mice. Results: The results indicated that the ethanolic extract of leaves of H. floribunda was active on Staphylococcus aureus ATCC 29213 and clinical strains of Staphylococcus aureus, Salmonella typhi and Klebsiella pneumoniae with MICs ranging from 0.62 to 1.25 mg/mL. The extract also showed significant parasitaemia suppression in a dose-dependent manner. In the acute toxicity assay, the oral administration of the extract to the mice did not affect the relative weight of vital organs, and there were no signs of toxicity or death during the study period. The LD50 of the tested extract was found to be greater than 5,000 mg/kg, indicating its safety. Conclusion: This study demonstrates the antibacterial and antimalarial activities of leaves of H. floribunda and then, supports its medicinal use in the treatment of microbial infections
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