Novel Machine Vision Tools Applied in Biomechatronic Tasks

AbstractThe article describes a novel machine vision tools for moving objects detection. The methods are based on spatial or frequency analysis and consecutive segmentation process. The moving objects are captured by video camera and image analysis is used for evaluation. This fact is very important in medical or biomechanical applications, when the moving objects are needed to determine patient's diagnosis or patient's behavior. Machine vision tools are applied for determination of movement frequency or trajectory

СОЦИАЛЬНЫЙ СЕРФИНГ МОЛОДЕЖИ КАК ФАКТОР СТАНОВЛЕНИЯ ГРАЖДАНСКОЙ СУБЪЕКТНОСТИ: СОЦИАЛЬНО-ФИЛОСОФСКИЙ АНАЛИЗ РЕЗУЛЬТАТОВ ФОРСАЙТА

The importence of the work is due to the insufficient knowledge about the phenomenon of social surfing and its contextual influence on the development of civic subjectivity. In the same time surfing is the relevance strategy in the youth environment. The overall propose of this work is to analyze some results of the foresight of the formation of civic subjectivity of young people in conditions of social surfing. The goal is a socio-philosophical assessment of the risks and opportunities for social surfing, including for the development of civic subjectivity in Russia based on the results of foresight. The methodology of the foresight (which had conducted in 2018 in Yekaterinburg) is based on the Rapid Foresight, but does not fully meet it, it is adapted to the specifics of the subject. The results showed potential of social surfing for the development of citizenship in the areas of politics, education, IT-technologies and personal development, which can be realized in the case of working with young people in the logic of decentralization. Conclusions are consistent with modern socio-philosophical concepts (plurality, heterarchy, social intelligence, mobility). The identified risks are associated with attempts to centralize youth activities, which could lead to an increase in formalism and the alienation of part of the youth contingent.Актуальность работы обуславливается недостаточной изученностью феномена социального серфинга и его контекстуального влияния на становление гражданской субъектности на фоне релевантности данной стратегии в молодежной среде. Общая задача данной работы – анализ некоторых результатов форсайта становления гражданской субъектности молодежи в условиях социального серфинга.Цель – социально-философская оценка рисков и возможностей социального серфинга, в том числе для становления гражданской субъектности в России, на основании результатов форсайта. Методология форсайта, проведенного в 2018 г. в Екатеринбурге, представляет собой адаптацию «Быстрого форсайта» (Rapid Foresight). Результаты показали потенциал социального серфинга для становления гражданственности в сферах политики, образования, IT-технологий и личностного развития, который может быть реализован в случае работы с молодежью в логике децентрализации. Выводы соответствуют современным социально-философским концепциям (множественность, гетерархия, социальный интеллект, мобильность). Выявленные риски связаны с попытками централизовать молодежные активности, что приведет к росту формализма и отчуждению части молодежного контингента

Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP

An electrochemical genosensor for the detection and quantification of Karlodinium armiger is presented. The genosensor exploits tailed primers and ferrocene labelled dATP analogue to produce PCR products that can be directly hybridised on a gold electrode array and quantitatively measured using square wave voltammetry. Tailed primers consist of a sequence specific for the target, followed by a carbon spacer and a sequence specifically designed not to bind to genomic DNA, resulting in a duplex flanked by single stranded binding primers. The incorporation of the 7-(ferrocenylethynyl)-7-deaza-2′-deoxyadenosine triphosphate was optimised in terms of a compromise between maximum PCR efficiency and the limit of detection and sensitivity attainable using electrochemical detection via hybridisation of the tailed, ferrocene labelled PCR product. A limit of detection of 277aM with a linear range from 315aM to 10 fM starting DNA concentration and a sensitivity of 122 nA decade−1 was achieved. The system was successfully applied to the detection of genomic DNA in real seawater samples.info:eu-repo/semantics/acceptedVersio

Comparison of experimental results from three dual fluidized bed test facilities capturing CO2 with CaO

10th International Conference on Greenhouse Gas Control Technologies[EN] Postcombustion CO2 capture technologies using CaO as a regenerable solid sorbent have emerged as a promising route to reduce the electricity penalty and the cost of CO2 capture from flue gases of both new and existing fossil fuelled power plants. Rapid progress is taking place in the understanding of these processes at different levels. However, experimental information, validating the concept under continuous operating conditions similar to those expected for large-scale application, remain scarce. We present here a comparative analysis of the results obtained in three laboratory-scale dual fluidized bed (DFB) test facilities in Spain, Germany and Canada. The test facilities range from 10 to 75 kWth with riser heights between 4.5 and 12.4 m. They have been operated to capture CO2 with CaO from simulated flue gases in the bubbling, turbulent and fast fluidization fluid-dynamic regimes. The carbonator reactors are interconnected with regenerators, where the CaCO3 decomposition has been conducted continuously and semi-continuously, operated in both air-combustion and oxy-combustion modes. Many stationary and non-stationary states have been achieved at different combinations of the key operating parameters (e.g. calcium looping ratio). All DFB test facilities showed a carbon balance closure of high quality in most tests. The trends of CO2 capture efficiency with respect to operating conditions and sorbent characteristics are compared and a discussion is made on the most appropriate methodology to conduct future tests under a joint new FP7 project (CaOling) that aims at the rapid scaling up of the calcium looping technology.This work is being funded by the European Commision 7th Framework Programme under the CaOling Project.Peer reviewe

The System of Neutrophil Elastase and the Plasma Level of MMP-7 in Children with Pulmonary Arterial Hypertension and Chronic Cor Pulmonale

A significant increase in the activity of neutrophil elastase (NE) and anti-NE-protection in the plasma were detected in children having bronchopulmonary dysplasia (BPD) complicated by pulmonary arterial hypertension (PAH) and chronic cor pulmonale (CCP). The changes revealed were more pronounced in patients with CCP. The plasma concentration of the NE was slightly reduced, which was probably associated with the activation of anti-NE and an increase in the α1-antitrypsin level. A gradual increase was noted in the plasma level of the matrix metalloproteinase-7 (MMP-7) in patients with an increase in the severity of the condition. In patients with cystic fibrosis (with and without CCP), the pronounced increase in the MMP-7 level was observed. In patients with cystic fibrosis (CF), even without the additional complication with PAH and CCP, the MMP-7 level was significantly higher than in those with congenital broncho-pulmonary malformations (CBPM). The difference was increased in those patients with PAH and reached a maximum in those with CCP

Correlation between Antioxidant Enzymes Activity and Intraerythrocyte Concentration of Fe, Mg, Zn, Cu in Pulmonary Arterial Hypertension and Cor Pulmonale in Children with Congenital Lung Disease and Cystic Fibrosis

Significant changes in the levels of the potential prooxidant Cu (increase) and the antioxidant Zn (decrease) in plasma were revealed in children having bronchopulmonary dysplasia (BPD) complicated by pulmonary arterial hypertension (PAH) and chronic cor pulmonale (CCP) when compared with the control. The Zn / Cu ratio in the blood plasma of patients with BPD, especially in CCP, was found to be lower than in the control group (p<0.001). This could indicate the activation of the prooxidant processes; simultaneously, the total antioxidant status (AOS) decreased. No significant increase in the intracellular free (“ionized” (i)) form of magnesium (iMg) was found; in fact, the concentration of iFe in all the patient groups was higher than in the control. An increase in the iCu and iZn levels (nonprotein-bound) was observed in the blood cells of the affected children. A significant increase in the glutathione peroxidase activity in the CCP patients may indicate an accumulation of organic peroxides, and partially compensate for the lesser activity of superoxide dismutase (SOD) and other antioxidants. The Zn / Cu and iZn/ iCu ratios were reduced in patients with CCP when compared with patients with PD without CCP

Electrochemical Detection of Single-Nucleotide Polymorphism Associated with Rifampicin Resistance in Mycobacterium tuberculosis Using Solid-Phase Primer Elongation with Ferrocene-Linked Redox-Labeled Nucleotides.

Here, we report the electrochemical detection of single-point mutations using solid-phase isothermal primer elongation with redox-labeled oligonucleotides. A single-base mutation associated with resistance to rifampicin, an antibiotic commonly used for the treatment of Mycobacterium tuberculosis, was used as a model system to demonstrate a proof-of-concept of the approach. Four 5'-thiolated primers, designed to be complementary with the same fragment of the target sequence and differing only in the last base, addressing the polymorphic site, were self-assembled via chemisorption on individual gold electrodes of an array. Following hybridization with single-stranded DNA, Klenow (exo-) DNA polymerase-mediated primer extension with ferrocene-labeled 2'-deoxyribonucleoside triphosphates (dNFcTPs) was only observed to proceed at the electrode where there was full complementarity between the surface-tethered probe and the target DNA being interrogated. We tested all four ferrocenylethynyl-linked dNTPs and optimized the ratio of labeled/natural nucleotides to achieve maximum sensitivity. Following a 20 min hybridization step, Klenow (exo-) DNA polymerase-mediated primer elongation at 37 °C for 5 min was optimal for the enzymatic incorporation of a ferrocene-labeled nucleotide, achieving unequivocal electrochemical detection of a single-point mutation in 14 samples of genomic DNA extracted from Mycobacterium tuberculosis strains. The approach is rapid, cost-effective, facile, and can be extended to multiplexed electrochemical single-point mutation genotyping